LOCUS       BBa_K654036             1134 bp    DNA     linear       08-Dec-2025
DEFINITION  Anderson 1.0 Promoter Controlled Renilla Luciferase
ACCESSION   BBa_K654036
VERSION     BBa_K654036.1  
FEATURES             Location/Qualifiers
     misc_feature    1..6
                     /label=-35 box
     promoter        1..35
                     /label=Anderson 1.0 Promoter J23100
     misc_feature    24..29
                     /label=-10 box
     promoter        1..35
                     /label=BBa_K654030
     misc_feature    48..51
                     /label=conserved
     RBS             44..55
                     /label=BBa_B0034
     misc_feature    62..997
                     /label=Rluc
     CDS             62..997
                     /label=Rluc
     misc_feature    62..997
                     /label=BBa_J52008
     stem_loop       1017..1060
                     /label=stem_loop
     misc_feature    1006..1085
                     /label=BBa_B0010
     terminator      1006..1085
                     /label=BBa_B0010
     stem_loop       1101..1120
                     /label=T7 TE
     misc_feature    1127..1127
                     /label=stop
     polyA_site      1121..1134
                     /label=polya
     misc_feature    1094..1134
                     /label=BBa_B0012
     terminator      1094..1134
                     /label=BBa_B0012
     terminator      1006..1134
                     /label=BBa_B0015
     misc_feature    62..1134
                     /label=BBa_K654034
     misc_feature    44..1134
                     /label=BBa_K654092
ORIGIN
        1 ttgacggcta gctcagtcct aggtacagtg ctagctacta gagaaagagg agaaatacta
       61 gatggcttcc aaggtgtacg accccgagca acgcaaacgc atgatcactg ggcctcagtg
      121 gtgggctcgc tgcaagcaaa tgaacgtgct ggactccttc atcaactact atgattccga
      181 gaagcacgcc gagaacgccg tgatttttct gcatggtaac gctgcctcca gctacctgtg
      241 gaggcacgtc gtgcctcaca tcgagcccgt ggctagatgc atcatccctg atctgatcgg
      301 aatgggtaag tccggcaaga gcgggaatgg ctcatatcgc ctcctggatc actacaagta
      361 cctcaccgct tggttcgagc tgctgaacct tccaaagaaa atcatctttg tgggccacga
      421 ctggggggct tgtctggcct ttcactactc ctacgagcac caagacaaga tcaaggccat
      481 cgtccatgct gagagtgtcg tggacgtgat cgagtcctgg gacgagtggc ctgacatcga
      541 ggaggatatc gccctgatca agagcgaaga gggcgagaaa atggtgcttg agaataactt
      601 cttcgtcgag accatgctcc caagcaagat catgcggaaa ctggagcctg aggagttcgc
      661 tgcctacctg gagccattca aggagaaggg cgaggttaga cggcctaccc tctcctggcc
      721 tcgcgagatc cctctcgtta agggaggcaa gcccgacgtc gtccagattg tccgcaacta
      781 caacgcctac cttcgggcca gcgacgatct gcctaagatg ttcatcgagt ccgaccctgg
      841 gttcttttcc aacgctattg tcgagggagc taagaagttc cctaacaccg agttcgtgaa
      901 ggtgaagggc ctccacttca gccaggagga cgctccagat gaaatgggta agtacatcaa
      961 gagcttcgtg gagcgcgtgc tgaagaacga gcagtaatac tagagccagg catcaaataa
     1021 aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg
     1081 ctctctacta gagtcacact ggctcacctt cgggtgggcc tttctgcgtt tata
//