LOCUS       BBa_K654039             1851 bp    DNA     linear       15-Nov-2024
DEFINITION  Anderson 1.0 Promoter Controlled Firefly Luciferase
ACCESSION   BBa_K654039
VERSION     BBa_K654039.1  
FEATURES             Location/Qualifiers
     misc_feature    1..6
                     /label=-35 box
     promoter        1..35
                     /label=Anderson 1.0 Promoter J23100
     misc_feature    24..29
                     /label=-10 box
     promoter        1..35
                     /label=BBa_K654030
     misc_feature    48..51
                     /label=conserved
     RBS             44..55
                     /label=BBa_B0034
     CDS             62..1714
                     /label=BBa_I712019
     stem_loop       1734..1777
                     /label=stem_loop
     misc_feature    1723..1802
                     /label=BBa_B0010
     terminator      1723..1802
                     /label=BBa_B0010
     stem_loop       1818..1837
                     /label=T7 TE
     misc_feature    1844..1844
                     /label=stop
     polyA_site      1838..1851
                     /label=polya
     misc_feature    1811..1851
                     /label=BBa_B0012
     terminator      1811..1851
                     /label=BBa_B0012
     terminator      1723..1851
                     /label=BBa_B0015
     misc_feature    62..1851
                     /label=BBa_K654033
     misc_feature    44..1851
                     /label=BBa_K654042
ORIGIN
        1 ttgacggcta gctcagtcct aggtacagtg ctagctacta gagaaagagg agaaatacta
       61 gatggaagac gccaaaaaca taaagaaagg cccggcgcca ttctatccgc tggaagatgg
      121 aaccgctgga gagcaactgc ataaggctat gaagagatac gccctggttc ctggaacaat
      181 tgcttttaca gatgcacata tcgaggtgga catcacttac gctgagtact tcgaaatgtc
      241 cgttcggttg gcagaagcta tgaaacgata tgggctgaat acaaatcaca gaatcgtcgt
      301 atgcagtgaa aactctcttc aattctttat gccggtgttg ggcgcgttat ttatcggagt
      361 tgcagttgcg cccgcgaacg acatttataa tgaacgtgaa ttgctcaaca gtatgggcat
      421 ttcgcagcct accgtggtgt tcgtttccaa aaaggggttg caaaaaattt tgaacgtgca
      481 aaaaaagctc ccaatcatcc aaaaaattat tatcatggat tctaaaacgg attaccaggg
      541 atttcagtcg atgtacacgt tcgtcacatc tcatctacct cccggtttta atgaatacga
      601 ttttgtgcca gagtccttcg atagggacaa gacaattgca ctgatcatga actcctctgg
      661 atctactggt ctgcctaaag gtgtcgctct gcctcataga actgcctgcg tgagattctc
      721 gcatgccaga gatcctattt ttggcaatca aatcattccg gatactgcga ttttaagtgt
      781 tgttccattc catcacggtt ttggaatgtt tactacactc ggatatttga tatgtggatt
      841 tcgagtcgtc ttaatgtata gatttgaaga agagctgttt ctgaggagcc ttcaggatta
      901 caagattcaa agtgcgctgc tggtgccaac cctattctcc ttcttcgcca aaagcactct
      961 gattgacaaa tacgatttat ctaatttaca cgaaattgct tctggtggcg ctcccctctc
     1021 taaggaagtc ggggaagcgg ttgccaagag gttccatctg ccaggtatca ggcaaggata
     1081 tgggctcact gagactacat cagctattct gattacaccc gagggggatg ataaaccggg
     1141 cgcggtcggt aaagttgttc cattttttga agcgaaggtt gtggatctgg ataccgggaa
     1201 aacgctgggc gttaatcaaa gaggcgaact gtgtgtgaga ggtcctatga ttatgtccgg
     1261 ttatgtaaac aatccggaag cgaccaacgc cttgattgac aaggatggat ggctacattc
     1321 tggagacata gcttactggg acgaagacga acacttcttc atcgttgacc gcctgaagtc
     1381 tctgattaag tacaaaggct atcaggtggc tcccgctgaa ttggaatcca tcttgctcca
     1441 acaccccaac atcttcgacg caggtgtcgc aggtcttccc gacgatgacg ccggtgaact
     1501 tcccgccgcc gttgttgttt tggagcacgg aaagacgatg acggaaaaag agatcgtgga
     1561 ttacgtcgcc agtcaagtaa caaccgcgaa aaagttgcgc ggaggagttg tgtttgtgga
     1621 cgaagtaccg aaaggtctta ccggaaaact cgacgcaaga aaaatcagag agatcctcat
     1681 aaaggccaag aagggcggaa agatcgccgt gtaatactag agccaggcat caaataaaac
     1741 gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc
     1801 tctactagag tcacactggc tcaccttcgg gtgggccttt ctgcgtttat a
//