LOCUS       BBa_K654040             1883 bp    DNA     linear       01-Jun-2024
DEFINITION  Synechocystis psbA2 controlled Firefly Luciferase
ACCESSION   BBa_K654040
VERSION     BBa_K654040.1  
FEATURES             Location/Qualifiers
     promoter        1..38
                     /label=psbA2 promoter
     RBS             71..80
                     /label=putative rbs
     misc_feature    3..8
                     /label=-35 box
     misc_feature    27..32
                     /label=-10 box
     misc_feature    39..39
                     /label=+1 Transcription Start Point
     promoter        1..87
                     /label=BBa_K390017
     CDS             94..1746
                     /label=BBa_I712019
     stem_loop       1766..1809
                     /label=stem_loop
     misc_feature    1755..1834
                     /label=BBa_B0010
     terminator      1755..1834
                     /label=BBa_B0010
     stem_loop       1850..1869
                     /label=T7 TE
     misc_feature    1876..1876
                     /label=stop
     polyA_site      1870..1883
                     /label=polya
     misc_feature    1843..1883
                     /label=BBa_B0012
     terminator      1843..1883
                     /label=BBa_B0012
     terminator      1755..1883
                     /label=BBa_B0015
     misc_feature    94..1883
                     /label=BBa_K654033
ORIGIN
        1 gctttacaaa actctcatta atcctttaga ctaagtttag tcagttccaa tctgaacatc
       61 gacaaataca taaggaatta taaccaatac tagatggaag acgccaaaaa cataaagaaa
      121 ggcccggcgc cattctatcc gctggaagat ggaaccgctg gagagcaact gcataaggct
      181 atgaagagat acgccctggt tcctggaaca attgctttta cagatgcaca tatcgaggtg
      241 gacatcactt acgctgagta cttcgaaatg tccgttcggt tggcagaagc tatgaaacga
      301 tatgggctga atacaaatca cagaatcgtc gtatgcagtg aaaactctct tcaattcttt
      361 atgccggtgt tgggcgcgtt atttatcgga gttgcagttg cgcccgcgaa cgacatttat
      421 aatgaacgtg aattgctcaa cagtatgggc atttcgcagc ctaccgtggt gttcgtttcc
      481 aaaaaggggt tgcaaaaaat tttgaacgtg caaaaaaagc tcccaatcat ccaaaaaatt
      541 attatcatgg attctaaaac ggattaccag ggatttcagt cgatgtacac gttcgtcaca
      601 tctcatctac ctcccggttt taatgaatac gattttgtgc cagagtcctt cgatagggac
      661 aagacaattg cactgatcat gaactcctct ggatctactg gtctgcctaa aggtgtcgct
      721 ctgcctcata gaactgcctg cgtgagattc tcgcatgcca gagatcctat ttttggcaat
      781 caaatcattc cggatactgc gattttaagt gttgttccat tccatcacgg ttttggaatg
      841 tttactacac tcggatattt gatatgtgga tttcgagtcg tcttaatgta tagatttgaa
      901 gaagagctgt ttctgaggag ccttcaggat tacaagattc aaagtgcgct gctggtgcca
      961 accctattct ccttcttcgc caaaagcact ctgattgaca aatacgattt atctaattta
     1021 cacgaaattg cttctggtgg cgctcccctc tctaaggaag tcggggaagc ggttgccaag
     1081 aggttccatc tgccaggtat caggcaagga tatgggctca ctgagactac atcagctatt
     1141 ctgattacac ccgaggggga tgataaaccg ggcgcggtcg gtaaagttgt tccatttttt
     1201 gaagcgaagg ttgtggatct ggataccggg aaaacgctgg gcgttaatca aagaggcgaa
     1261 ctgtgtgtga gaggtcctat gattatgtcc ggttatgtaa acaatccgga agcgaccaac
     1321 gccttgattg acaaggatgg atggctacat tctggagaca tagcttactg ggacgaagac
     1381 gaacacttct tcatcgttga ccgcctgaag tctctgatta agtacaaagg ctatcaggtg
     1441 gctcccgctg aattggaatc catcttgctc caacacccca acatcttcga cgcaggtgtc
     1501 gcaggtcttc ccgacgatga cgccggtgaa cttcccgccg ccgttgttgt tttggagcac
     1561 ggaaagacga tgacggaaaa agagatcgtg gattacgtcg ccagtcaagt aacaaccgcg
     1621 aaaaagttgc gcggaggagt tgtgtttgtg gacgaagtac cgaaaggtct taccggaaaa
     1681 ctcgacgcaa gaaaaatcag agagatcctc ataaaggcca agaagggcgg aaagatcgcc
     1741 gtgtaatact agagccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt
     1801 tcgttttatc tgttgtttgt cggtgaacgc tctctactag agtcacactg gctcaccttc
     1861 gggtgggcct ttctgcgttt ata
//