LOCUS       BBa_K654042             1808 bp    DNA     linear       22-Dec-2024
DEFINITION  E. coli 1.0 RBS + Firefly Luciferase + Terminator
ACCESSION   BBa_K654042
VERSION     BBa_K654042.1  
FEATURES             Location/Qualifiers
     misc_feature    5..8
                     /label=conserved
     RBS             1..12
                     /label=BBa_B0034
     CDS             19..1671
                     /label=BBa_I712019
     stem_loop       1691..1734
                     /label=stem_loop
     misc_feature    1680..1759
                     /label=BBa_B0010
     terminator      1680..1759
                     /label=BBa_B0010
     stem_loop       1775..1794
                     /label=T7 TE
     misc_feature    1801..1801
                     /label=stop
     polyA_site      1795..1808
                     /label=polya
     misc_feature    1768..1808
                     /label=BBa_B0012
     terminator      1768..1808
                     /label=BBa_B0012
     terminator      1680..1808
                     /label=BBa_B0015
     misc_feature    19..1808
                     /label=BBa_K654033
ORIGIN
        1 aaagaggaga aatactagat ggaagacgcc aaaaacataa agaaaggccc ggcgccattc
       61 tatccgctgg aagatggaac cgctggagag caactgcata aggctatgaa gagatacgcc
      121 ctggttcctg gaacaattgc ttttacagat gcacatatcg aggtggacat cacttacgct
      181 gagtacttcg aaatgtccgt tcggttggca gaagctatga aacgatatgg gctgaataca
      241 aatcacagaa tcgtcgtatg cagtgaaaac tctcttcaat tctttatgcc ggtgttgggc
      301 gcgttattta tcggagttgc agttgcgccc gcgaacgaca tttataatga acgtgaattg
      361 ctcaacagta tgggcatttc gcagcctacc gtggtgttcg tttccaaaaa ggggttgcaa
      421 aaaattttga acgtgcaaaa aaagctccca atcatccaaa aaattattat catggattct
      481 aaaacggatt accagggatt tcagtcgatg tacacgttcg tcacatctca tctacctccc
      541 ggttttaatg aatacgattt tgtgccagag tccttcgata gggacaagac aattgcactg
      601 atcatgaact cctctggatc tactggtctg cctaaaggtg tcgctctgcc tcatagaact
      661 gcctgcgtga gattctcgca tgccagagat cctatttttg gcaatcaaat cattccggat
      721 actgcgattt taagtgttgt tccattccat cacggttttg gaatgtttac tacactcgga
      781 tatttgatat gtggatttcg agtcgtctta atgtatagat ttgaagaaga gctgtttctg
      841 aggagccttc aggattacaa gattcaaagt gcgctgctgg tgccaaccct attctccttc
      901 ttcgccaaaa gcactctgat tgacaaatac gatttatcta atttacacga aattgcttct
      961 ggtggcgctc ccctctctaa ggaagtcggg gaagcggttg ccaagaggtt ccatctgcca
     1021 ggtatcaggc aaggatatgg gctcactgag actacatcag ctattctgat tacacccgag
     1081 ggggatgata aaccgggcgc ggtcggtaaa gttgttccat tttttgaagc gaaggttgtg
     1141 gatctggata ccgggaaaac gctgggcgtt aatcaaagag gcgaactgtg tgtgagaggt
     1201 cctatgatta tgtccggtta tgtaaacaat ccggaagcga ccaacgcctt gattgacaag
     1261 gatggatggc tacattctgg agacatagct tactgggacg aagacgaaca cttcttcatc
     1321 gttgaccgcc tgaagtctct gattaagtac aaaggctatc aggtggctcc cgctgaattg
     1381 gaatccatct tgctccaaca ccccaacatc ttcgacgcag gtgtcgcagg tcttcccgac
     1441 gatgacgccg gtgaacttcc cgccgccgtt gttgttttgg agcacggaaa gacgatgacg
     1501 gaaaaagaga tcgtggatta cgtcgccagt caagtaacaa ccgcgaaaaa gttgcgcgga
     1561 ggagttgtgt ttgtggacga agtaccgaaa ggtcttaccg gaaaactcga cgcaagaaaa
     1621 atcagagaga tcctcataaa ggccaagaag ggcggaaaga tcgccgtgta atactagagc
     1681 caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg
     1741 tttgtcggtg aacgctctct actagagtca cactggctca ccttcgggtg ggcctttctg
     1801 cgtttata
//