LOCUS       BBa_K654043             1845 bp    DNA     linear       23-Dec-2024
DEFINITION  psbA2 5'UTR Native RBS + Firefly Luciferase + Terminator
ACCESSION   BBa_K654043
VERSION     BBa_K654043.1  
FEATURES             Location/Qualifiers
     misc_feature    1..49
                     /label=5' UTR with native RBS
     RBS             35..38
                     /label=SDS core consensus
     RBS             33..42
                     /label=native RBS
     RBS             1..49
                     /label=BBa_K390001
     CDS             56..1708
                     /label=BBa_I712019
     stem_loop       1728..1771
                     /label=stem_loop
     misc_feature    1717..1796
                     /label=BBa_B0010
     terminator      1717..1796
                     /label=BBa_B0010
     stem_loop       1812..1831
                     /label=T7 TE
     misc_feature    1838..1838
                     /label=stop
     polyA_site      1832..1845
                     /label=polya
     misc_feature    1805..1845
                     /label=BBa_B0012
     terminator      1805..1845
                     /label=BBa_B0012
     terminator      1717..1845
                     /label=BBa_B0015
     misc_feature    56..1845
                     /label=BBa_K654033
ORIGIN
        1 agtcagttcc aatctgaaca tcgacaaata cataaggaat tataaccaat actagatgga
       61 agacgccaaa aacataaaga aaggcccggc gccattctat ccgctggaag atggaaccgc
      121 tggagagcaa ctgcataagg ctatgaagag atacgccctg gttcctggaa caattgcttt
      181 tacagatgca catatcgagg tggacatcac ttacgctgag tacttcgaaa tgtccgttcg
      241 gttggcagaa gctatgaaac gatatgggct gaatacaaat cacagaatcg tcgtatgcag
      301 tgaaaactct cttcaattct ttatgccggt gttgggcgcg ttatttatcg gagttgcagt
      361 tgcgcccgcg aacgacattt ataatgaacg tgaattgctc aacagtatgg gcatttcgca
      421 gcctaccgtg gtgttcgttt ccaaaaaggg gttgcaaaaa attttgaacg tgcaaaaaaa
      481 gctcccaatc atccaaaaaa ttattatcat ggattctaaa acggattacc agggatttca
      541 gtcgatgtac acgttcgtca catctcatct acctcccggt tttaatgaat acgattttgt
      601 gccagagtcc ttcgataggg acaagacaat tgcactgatc atgaactcct ctggatctac
      661 tggtctgcct aaaggtgtcg ctctgcctca tagaactgcc tgcgtgagat tctcgcatgc
      721 cagagatcct atttttggca atcaaatcat tccggatact gcgattttaa gtgttgttcc
      781 attccatcac ggttttggaa tgtttactac actcggatat ttgatatgtg gatttcgagt
      841 cgtcttaatg tatagatttg aagaagagct gtttctgagg agccttcagg attacaagat
      901 tcaaagtgcg ctgctggtgc caaccctatt ctccttcttc gccaaaagca ctctgattga
      961 caaatacgat ttatctaatt tacacgaaat tgcttctggt ggcgctcccc tctctaagga
     1021 agtcggggaa gcggttgcca agaggttcca tctgccaggt atcaggcaag gatatgggct
     1081 cactgagact acatcagcta ttctgattac acccgagggg gatgataaac cgggcgcggt
     1141 cggtaaagtt gttccatttt ttgaagcgaa ggttgtggat ctggataccg ggaaaacgct
     1201 gggcgttaat caaagaggcg aactgtgtgt gagaggtcct atgattatgt ccggttatgt
     1261 aaacaatccg gaagcgacca acgccttgat tgacaaggat ggatggctac attctggaga
     1321 catagcttac tgggacgaag acgaacactt cttcatcgtt gaccgcctga agtctctgat
     1381 taagtacaaa ggctatcagg tggctcccgc tgaattggaa tccatcttgc tccaacaccc
     1441 caacatcttc gacgcaggtg tcgcaggtct tcccgacgat gacgccggtg aacttcccgc
     1501 cgccgttgtt gttttggagc acggaaagac gatgacggaa aaagagatcg tggattacgt
     1561 cgccagtcaa gtaacaaccg cgaaaaagtt gcgcggagga gttgtgtttg tggacgaagt
     1621 accgaaaggt cttaccggaa aactcgacgc aagaaaaatc agagagatcc tcataaaggc
     1681 caagaagggc ggaaagatcg ccgtgtaata ctagagccag gcatcaaata aaacgaaagg
     1741 ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact
     1801 agagtcacac tggctcacct tcgggtgggc ctttctgcgt ttata
//