LOCUS BBa_K654045 2950 bp DNA linear 22-Dec-2024 DEFINITION Promoter Tester Anderson E.coli 1.0 Reference Dual Luciferase Assay ACCESSION BBa_K654045 VERSION BBa_K654045.1 FEATURES Location/Qualifiers misc_feature 5..8 /label=conserved RBS 1..12 /label=BBa_B0034 CDS 19..1671 /label=BBa_I712019 stem_loop 1691..1734 /label=stem_loop misc_feature 1680..1759 /label=BBa_B0010 terminator 1680..1759 /label=BBa_B0010 stem_loop 1775..1794 /label=T7 TE misc_feature 1801..1801 /label=stop polyA_site 1795..1808 /label=polya misc_feature 1768..1808 /label=BBa_B0012 terminator 1768..1808 /label=BBa_B0012 terminator 1680..1808 /label=BBa_B0015 misc_feature 19..1808 /label=BBa_K654033 misc_feature 1..1808 /label=BBa_K654042 misc_feature 1817..1822 /label=-35 box promoter 1817..1851 /label=Anderson 1.0 Promoter J23100 misc_feature 1840..1845 /label=-10 box promoter 1817..1851 /label=BBa_K654030 misc_feature 1864..1867 /label=conserved RBS 1860..1871 /label=BBa_B0034 misc_feature 1878..2813 /label=Rluc CDS 1878..2813 /label=Rluc misc_feature 1878..2813 /label=BBa_J52008 stem_loop 2833..2876 /label=stem_loop misc_feature 2822..2901 /label=BBa_B0010 terminator 2822..2901 /label=BBa_B0010 stem_loop 2917..2936 /label=T7 TE misc_feature 2943..2943 /label=stop polyA_site 2937..2950 /label=polya misc_feature 2910..2950 /label=BBa_B0012 terminator 2910..2950 /label=BBa_B0012 terminator 2822..2950 /label=BBa_B0015 misc_feature 1878..2950 /label=BBa_K654034 misc_feature 1860..2950 /label=BBa_K654092 misc_feature 1817..2950 /label=BBa_K654036 ORIGIN 1 aaagaggaga aatactagat ggaagacgcc aaaaacataa agaaaggccc ggcgccattc 61 tatccgctgg aagatggaac cgctggagag caactgcata aggctatgaa gagatacgcc 121 ctggttcctg gaacaattgc ttttacagat gcacatatcg aggtggacat cacttacgct 181 gagtacttcg aaatgtccgt tcggttggca gaagctatga aacgatatgg gctgaataca 241 aatcacagaa tcgtcgtatg cagtgaaaac tctcttcaat tctttatgcc ggtgttgggc 301 gcgttattta tcggagttgc agttgcgccc gcgaacgaca tttataatga acgtgaattg 361 ctcaacagta tgggcatttc gcagcctacc gtggtgttcg tttccaaaaa ggggttgcaa 421 aaaattttga acgtgcaaaa aaagctccca atcatccaaa aaattattat catggattct 481 aaaacggatt accagggatt tcagtcgatg tacacgttcg tcacatctca tctacctccc 541 ggttttaatg aatacgattt tgtgccagag tccttcgata gggacaagac aattgcactg 601 atcatgaact cctctggatc tactggtctg cctaaaggtg tcgctctgcc tcatagaact 661 gcctgcgtga gattctcgca tgccagagat cctatttttg gcaatcaaat cattccggat 721 actgcgattt taagtgttgt tccattccat cacggttttg gaatgtttac tacactcgga 781 tatttgatat gtggatttcg agtcgtctta atgtatagat ttgaagaaga gctgtttctg 841 aggagccttc aggattacaa gattcaaagt gcgctgctgg tgccaaccct attctccttc 901 ttcgccaaaa gcactctgat tgacaaatac gatttatcta atttacacga aattgcttct 961 ggtggcgctc ccctctctaa ggaagtcggg gaagcggttg ccaagaggtt ccatctgcca 1021 ggtatcaggc aaggatatgg gctcactgag actacatcag ctattctgat tacacccgag 1081 ggggatgata aaccgggcgc ggtcggtaaa gttgttccat tttttgaagc gaaggttgtg 1141 gatctggata ccgggaaaac gctgggcgtt aatcaaagag gcgaactgtg tgtgagaggt 1201 cctatgatta tgtccggtta tgtaaacaat ccggaagcga ccaacgcctt gattgacaag 1261 gatggatggc tacattctgg agacatagct tactgggacg aagacgaaca cttcttcatc 1321 gttgaccgcc tgaagtctct gattaagtac aaaggctatc aggtggctcc cgctgaattg 1381 gaatccatct tgctccaaca ccccaacatc ttcgacgcag gtgtcgcagg tcttcccgac 1441 gatgacgccg gtgaacttcc cgccgccgtt gttgttttgg agcacggaaa gacgatgacg 1501 gaaaaagaga tcgtggatta cgtcgccagt caagtaacaa ccgcgaaaaa gttgcgcgga 1561 ggagttgtgt ttgtggacga agtaccgaaa ggtcttaccg gaaaactcga cgcaagaaaa 1621 atcagagaga tcctcataaa ggccaagaag ggcggaaaga tcgccgtgta atactagagc 1681 caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg 1741 tttgtcggtg aacgctctct actagagtca cactggctca ccttcgggtg ggcctttctg 1801 cgtttatata ctagagttga cggctagctc agtcctaggt acagtgctag ctactagaga 1861 aagaggagaa atactagatg gcttccaagg tgtacgaccc cgagcaacgc aaacgcatga 1921 tcactgggcc tcagtggtgg gctcgctgca agcaaatgaa cgtgctggac tccttcatca 1981 actactatga ttccgagaag cacgccgaga acgccgtgat ttttctgcat ggtaacgctg 2041 cctccagcta cctgtggagg cacgtcgtgc ctcacatcga gcccgtggct agatgcatca 2101 tccctgatct gatcggaatg ggtaagtccg gcaagagcgg gaatggctca tatcgcctcc 2161 tggatcacta caagtacctc accgcttggt tcgagctgct gaaccttcca aagaaaatca 2221 tctttgtggg ccacgactgg ggggcttgtc tggcctttca ctactcctac gagcaccaag 2281 acaagatcaa ggccatcgtc catgctgaga gtgtcgtgga cgtgatcgag tcctgggacg 2341 agtggcctga catcgaggag gatatcgccc tgatcaagag cgaagagggc gagaaaatgg 2401 tgcttgagaa taacttcttc gtcgagacca tgctcccaag caagatcatg cggaaactgg 2461 agcctgagga gttcgctgcc tacctggagc cattcaagga gaagggcgag gttagacggc 2521 ctaccctctc ctggcctcgc gagatccctc tcgttaaggg aggcaagccc gacgtcgtcc 2581 agattgtccg caactacaac gcctaccttc gggccagcga cgatctgcct aagatgttca 2641 tcgagtccga ccctgggttc ttttccaacg ctattgtcga gggagctaag aagttcccta 2701 acaccgagtt cgtgaaggtg aagggcctcc acttcagcca ggaggacgct ccagatgaaa 2761 tgggtaagta catcaagagc ttcgtggagc gcgtgctgaa gaacgagcag taatactaga 2821 gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt 2881 tgtttgtcgg tgaacgctct ctactagagt cacactggct caccttcggg tgggcctttc 2941 tgcgtttata //