LOCUS       BBa_K654046             2932 bp    DNA     linear       10-Dec-2025
DEFINITION  Promoter/RBS Tester Anderson 1.0 Reference Dual Luciferase Assay
ACCESSION   BBa_K654046
VERSION     BBa_K654046.1  
FEATURES             Location/Qualifiers
     CDS             1..1653
                     /label=BBa_I712019
     stem_loop       1673..1716
                     /label=stem_loop
     misc_feature    1662..1741
                     /label=BBa_B0010
     terminator      1662..1741
                     /label=BBa_B0010
     stem_loop       1757..1776
                     /label=T7 TE
     misc_feature    1783..1783
                     /label=stop
     polyA_site      1777..1790
                     /label=polya
     misc_feature    1750..1790
                     /label=BBa_B0012
     terminator      1750..1790
                     /label=BBa_B0012
     terminator      1662..1790
                     /label=BBa_B0015
     misc_feature    1..1790
                     /label=BBa_K654033
     misc_feature    1799..1804
                     /label=-35 box
     promoter        1799..1833
                     /label=Anderson 1.0 Promoter J23100
     misc_feature    1822..1827
                     /label=-10 box
     promoter        1799..1833
                     /label=BBa_K654030
     misc_feature    1846..1849
                     /label=conserved
     RBS             1842..1853
                     /label=BBa_B0034
     misc_feature    1860..2795
                     /label=Rluc
     CDS             1860..2795
                     /label=Rluc
     misc_feature    1860..2795
                     /label=BBa_J52008
     stem_loop       2815..2858
                     /label=stem_loop
     misc_feature    2804..2883
                     /label=BBa_B0010
     terminator      2804..2883
                     /label=BBa_B0010
     stem_loop       2899..2918
                     /label=T7 TE
     misc_feature    2925..2925
                     /label=stop
     polyA_site      2919..2932
                     /label=polya
     misc_feature    2892..2932
                     /label=BBa_B0012
     terminator      2892..2932
                     /label=BBa_B0012
     terminator      2804..2932
                     /label=BBa_B0015
     misc_feature    1860..2932
                     /label=BBa_K654034
     misc_feature    1842..2932
                     /label=BBa_K654092
     misc_feature    1799..2932
                     /label=BBa_K654036
ORIGIN
        1 atggaagacg ccaaaaacat aaagaaaggc ccggcgccat tctatccgct ggaagatgga
       61 accgctggag agcaactgca taaggctatg aagagatacg ccctggttcc tggaacaatt
      121 gcttttacag atgcacatat cgaggtggac atcacttacg ctgagtactt cgaaatgtcc
      181 gttcggttgg cagaagctat gaaacgatat gggctgaata caaatcacag aatcgtcgta
      241 tgcagtgaaa actctcttca attctttatg ccggtgttgg gcgcgttatt tatcggagtt
      301 gcagttgcgc ccgcgaacga catttataat gaacgtgaat tgctcaacag tatgggcatt
      361 tcgcagccta ccgtggtgtt cgtttccaaa aaggggttgc aaaaaatttt gaacgtgcaa
      421 aaaaagctcc caatcatcca aaaaattatt atcatggatt ctaaaacgga ttaccaggga
      481 tttcagtcga tgtacacgtt cgtcacatct catctacctc ccggttttaa tgaatacgat
      541 tttgtgccag agtccttcga tagggacaag acaattgcac tgatcatgaa ctcctctgga
      601 tctactggtc tgcctaaagg tgtcgctctg cctcatagaa ctgcctgcgt gagattctcg
      661 catgccagag atcctatttt tggcaatcaa atcattccgg atactgcgat tttaagtgtt
      721 gttccattcc atcacggttt tggaatgttt actacactcg gatatttgat atgtggattt
      781 cgagtcgtct taatgtatag atttgaagaa gagctgtttc tgaggagcct tcaggattac
      841 aagattcaaa gtgcgctgct ggtgccaacc ctattctcct tcttcgccaa aagcactctg
      901 attgacaaat acgatttatc taatttacac gaaattgctt ctggtggcgc tcccctctct
      961 aaggaagtcg gggaagcggt tgccaagagg ttccatctgc caggtatcag gcaaggatat
     1021 gggctcactg agactacatc agctattctg attacacccg agggggatga taaaccgggc
     1081 gcggtcggta aagttgttcc attttttgaa gcgaaggttg tggatctgga taccgggaaa
     1141 acgctgggcg ttaatcaaag aggcgaactg tgtgtgagag gtcctatgat tatgtccggt
     1201 tatgtaaaca atccggaagc gaccaacgcc ttgattgaca aggatggatg gctacattct
     1261 ggagacatag cttactggga cgaagacgaa cacttcttca tcgttgaccg cctgaagtct
     1321 ctgattaagt acaaaggcta tcaggtggct cccgctgaat tggaatccat cttgctccaa
     1381 caccccaaca tcttcgacgc aggtgtcgca ggtcttcccg acgatgacgc cggtgaactt
     1441 cccgccgccg ttgttgtttt ggagcacgga aagacgatga cggaaaaaga gatcgtggat
     1501 tacgtcgcca gtcaagtaac aaccgcgaaa aagttgcgcg gaggagttgt gtttgtggac
     1561 gaagtaccga aaggtcttac cggaaaactc gacgcaagaa aaatcagaga gatcctcata
     1621 aaggccaaga agggcggaaa gatcgccgtg taatactaga gccaggcatc aaataaaacg
     1681 aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct
     1741 ctactagagt cacactggct caccttcggg tgggcctttc tgcgtttata tactagagtt
     1801 gacggctagc tcagtcctag gtacagtgct agctactaga gaaagaggag aaatactaga
     1861 tggcttccaa ggtgtacgac cccgagcaac gcaaacgcat gatcactggg cctcagtggt
     1921 gggctcgctg caagcaaatg aacgtgctgg actccttcat caactactat gattccgaga
     1981 agcacgccga gaacgccgtg atttttctgc atggtaacgc tgcctccagc tacctgtgga
     2041 ggcacgtcgt gcctcacatc gagcccgtgg ctagatgcat catccctgat ctgatcggaa
     2101 tgggtaagtc cggcaagagc gggaatggct catatcgcct cctggatcac tacaagtacc
     2161 tcaccgcttg gttcgagctg ctgaaccttc caaagaaaat catctttgtg ggccacgact
     2221 ggggggcttg tctggccttt cactactcct acgagcacca agacaagatc aaggccatcg
     2281 tccatgctga gagtgtcgtg gacgtgatcg agtcctggga cgagtggcct gacatcgagg
     2341 aggatatcgc cctgatcaag agcgaagagg gcgagaaaat ggtgcttgag aataacttct
     2401 tcgtcgagac catgctccca agcaagatca tgcggaaact ggagcctgag gagttcgctg
     2461 cctacctgga gccattcaag gagaagggcg aggttagacg gcctaccctc tcctggcctc
     2521 gcgagatccc tctcgttaag ggaggcaagc ccgacgtcgt ccagattgtc cgcaactaca
     2581 acgcctacct tcgggccagc gacgatctgc ctaagatgtt catcgagtcc gaccctgggt
     2641 tcttttccaa cgctattgtc gagggagcta agaagttccc taacaccgag ttcgtgaagg
     2701 tgaagggcct ccacttcagc caggaggacg ctccagatga aatgggtaag tacatcaaga
     2761 gcttcgtgga gcgcgtgctg aagaacgagc agtaatacta gagccaggca tcaaataaaa
     2821 cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct
     2881 ctctactaga gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta ta
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