LOCUS BBa_K654047 2993 bp DNA linear 23-Dec-2024 DEFINITION Promoter Control Anderson E.coli 1.0 Reference Dual Luciferase Assay ACCESSION BBa_K654047 VERSION BBa_K654047.1 FEATURES Location/Qualifiers misc_feature 1..6 /label=-35 box promoter 1..35 /label=Anderson 1.0 Promoter J23100 misc_feature 24..29 /label=-10 box promoter 1..35 /label=BBa_K654030 misc_feature 48..51 /label=conserved RBS 44..55 /label=BBa_B0034 CDS 62..1714 /label=BBa_I712019 stem_loop 1734..1777 /label=stem_loop misc_feature 1723..1802 /label=BBa_B0010 terminator 1723..1802 /label=BBa_B0010 stem_loop 1818..1837 /label=T7 TE misc_feature 1844..1844 /label=stop polyA_site 1838..1851 /label=polya misc_feature 1811..1851 /label=BBa_B0012 terminator 1811..1851 /label=BBa_B0012 terminator 1723..1851 /label=BBa_B0015 misc_feature 62..1851 /label=BBa_K654033 misc_feature 44..1851 /label=BBa_K654042 misc_feature 1..1851 /label=BBa_K654039 misc_feature 1860..1865 /label=-35 box promoter 1860..1894 /label=Anderson 1.0 Promoter J23100 misc_feature 1883..1888 /label=-10 box promoter 1860..1894 /label=BBa_K654030 misc_feature 1907..1910 /label=conserved RBS 1903..1914 /label=BBa_B0034 misc_feature 1921..2856 /label=Rluc CDS 1921..2856 /label=Rluc misc_feature 1921..2856 /label=BBa_J52008 stem_loop 2876..2919 /label=stem_loop misc_feature 2865..2944 /label=BBa_B0010 terminator 2865..2944 /label=BBa_B0010 stem_loop 2960..2979 /label=T7 TE misc_feature 2986..2986 /label=stop polyA_site 2980..2993 /label=polya misc_feature 2953..2993 /label=BBa_B0012 terminator 2953..2993 /label=BBa_B0012 terminator 2865..2993 /label=BBa_B0015 misc_feature 1921..2993 /label=BBa_K654034 misc_feature 1903..2993 /label=BBa_K654092 misc_feature 1860..2993 /label=BBa_K654036 ORIGIN 1 ttgacggcta gctcagtcct aggtacagtg ctagctacta gagaaagagg agaaatacta 61 gatggaagac gccaaaaaca taaagaaagg cccggcgcca ttctatccgc tggaagatgg 121 aaccgctgga gagcaactgc ataaggctat gaagagatac gccctggttc ctggaacaat 181 tgcttttaca gatgcacata tcgaggtgga catcacttac gctgagtact tcgaaatgtc 241 cgttcggttg gcagaagcta tgaaacgata tgggctgaat acaaatcaca gaatcgtcgt 301 atgcagtgaa aactctcttc aattctttat gccggtgttg ggcgcgttat ttatcggagt 361 tgcagttgcg cccgcgaacg acatttataa tgaacgtgaa ttgctcaaca gtatgggcat 421 ttcgcagcct accgtggtgt tcgtttccaa aaaggggttg caaaaaattt tgaacgtgca 481 aaaaaagctc ccaatcatcc aaaaaattat tatcatggat tctaaaacgg attaccaggg 541 atttcagtcg atgtacacgt tcgtcacatc tcatctacct cccggtttta atgaatacga 601 ttttgtgcca gagtccttcg atagggacaa gacaattgca ctgatcatga actcctctgg 661 atctactggt ctgcctaaag gtgtcgctct gcctcataga actgcctgcg tgagattctc 721 gcatgccaga gatcctattt ttggcaatca aatcattccg gatactgcga ttttaagtgt 781 tgttccattc catcacggtt ttggaatgtt tactacactc ggatatttga tatgtggatt 841 tcgagtcgtc ttaatgtata gatttgaaga agagctgttt ctgaggagcc ttcaggatta 901 caagattcaa agtgcgctgc tggtgccaac cctattctcc ttcttcgcca aaagcactct 961 gattgacaaa tacgatttat ctaatttaca cgaaattgct tctggtggcg ctcccctctc 1021 taaggaagtc ggggaagcgg ttgccaagag gttccatctg ccaggtatca ggcaaggata 1081 tgggctcact gagactacat cagctattct gattacaccc gagggggatg ataaaccggg 1141 cgcggtcggt aaagttgttc cattttttga agcgaaggtt gtggatctgg ataccgggaa 1201 aacgctgggc gttaatcaaa gaggcgaact gtgtgtgaga ggtcctatga ttatgtccgg 1261 ttatgtaaac aatccggaag cgaccaacgc cttgattgac aaggatggat ggctacattc 1321 tggagacata gcttactggg acgaagacga acacttcttc atcgttgacc gcctgaagtc 1381 tctgattaag tacaaaggct atcaggtggc tcccgctgaa ttggaatcca tcttgctcca 1441 acaccccaac atcttcgacg caggtgtcgc aggtcttccc gacgatgacg ccggtgaact 1501 tcccgccgcc gttgttgttt tggagcacgg aaagacgatg acggaaaaag agatcgtgga 1561 ttacgtcgcc agtcaagtaa caaccgcgaa aaagttgcgc ggaggagttg tgtttgtgga 1621 cgaagtaccg aaaggtctta ccggaaaact cgacgcaaga aaaatcagag agatcctcat 1681 aaaggccaag aagggcggaa agatcgccgt gtaatactag agccaggcat caaataaaac 1741 gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc 1801 tctactagag tcacactggc tcaccttcgg gtgggccttt ctgcgtttat atactagagt 1861 tgacggctag ctcagtccta ggtacagtgc tagctactag agaaagagga gaaatactag 1921 atggcttcca aggtgtacga ccccgagcaa cgcaaacgca tgatcactgg gcctcagtgg 1981 tgggctcgct gcaagcaaat gaacgtgctg gactccttca tcaactacta tgattccgag 2041 aagcacgccg agaacgccgt gatttttctg catggtaacg ctgcctccag ctacctgtgg 2101 aggcacgtcg tgcctcacat cgagcccgtg gctagatgca tcatccctga tctgatcgga 2161 atgggtaagt ccggcaagag cgggaatggc tcatatcgcc tcctggatca ctacaagtac 2221 ctcaccgctt ggttcgagct gctgaacctt ccaaagaaaa tcatctttgt gggccacgac 2281 tggggggctt gtctggcctt tcactactcc tacgagcacc aagacaagat caaggccatc 2341 gtccatgctg agagtgtcgt ggacgtgatc gagtcctggg acgagtggcc tgacatcgag 2401 gaggatatcg ccctgatcaa gagcgaagag ggcgagaaaa tggtgcttga gaataacttc 2461 ttcgtcgaga ccatgctccc aagcaagatc atgcggaaac tggagcctga ggagttcgct 2521 gcctacctgg agccattcaa ggagaagggc gaggttagac ggcctaccct ctcctggcct 2581 cgcgagatcc ctctcgttaa gggaggcaag cccgacgtcg tccagattgt ccgcaactac 2641 aacgcctacc ttcgggccag cgacgatctg cctaagatgt tcatcgagtc cgaccctggg 2701 ttcttttcca acgctattgt cgagggagct aagaagttcc ctaacaccga gttcgtgaag 2761 gtgaagggcc tccacttcag ccaggaggac gctccagatg aaatgggtaa gtacatcaag 2821 agcttcgtgg agcgcgtgct gaagaacgag cagtaatact agagccaggc atcaaataaa 2881 acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc 2941 tctctactag agtcacactg gctcaccttc gggtgggcct ttctgcgttt ata //