LOCUS       BBa_K654049             2964 bp    DNA     linear       13-Jun-2024
DEFINITION  Promoter/RBS Tester psbA2 Reference Dual Luciferase Assay
ACCESSION   BBa_K654049
VERSION     BBa_K654049.1  
FEATURES             Location/Qualifiers
     CDS             1..1653
                     /label=BBa_I712019
     stem_loop       1673..1716
                     /label=stem_loop
     misc_feature    1662..1741
                     /label=BBa_B0010
     terminator      1662..1741
                     /label=BBa_B0010
     stem_loop       1757..1776
                     /label=T7 TE
     misc_feature    1783..1783
                     /label=stop
     polyA_site      1777..1790
                     /label=polya
     misc_feature    1750..1790
                     /label=BBa_B0012
     terminator      1750..1790
                     /label=BBa_B0012
     terminator      1662..1790
                     /label=BBa_B0015
     misc_feature    1..1790
                     /label=BBa_K654033
     promoter        1799..1836
                     /label=psbA2 promoter
     RBS             1869..1878
                     /label=putative rbs
     misc_feature    1801..1806
                     /label=-35 box
     misc_feature    1825..1830
                     /label=-10 box
     misc_feature    1837..1837
                     /label=+1 Transcription Start Point
     promoter        1799..1885
                     /label=BBa_K390017
     misc_feature    1892..2827
                     /label=Rluc
     CDS             1892..2827
                     /label=Rluc
     misc_feature    1892..2827
                     /label=BBa_J52008
     stem_loop       2847..2890
                     /label=stem_loop
     misc_feature    2836..2915
                     /label=BBa_B0010
     terminator      2836..2915
                     /label=BBa_B0010
     stem_loop       2931..2950
                     /label=T7 TE
     misc_feature    2957..2957
                     /label=stop
     polyA_site      2951..2964
                     /label=polya
     misc_feature    2924..2964
                     /label=BBa_B0012
     terminator      2924..2964
                     /label=BBa_B0012
     terminator      2836..2964
                     /label=BBa_B0015
     misc_feature    1892..2964
                     /label=BBa_K654034
     misc_feature    1799..2964
                     /label=BBa_K654037
ORIGIN
        1 atggaagacg ccaaaaacat aaagaaaggc ccggcgccat tctatccgct ggaagatgga
       61 accgctggag agcaactgca taaggctatg aagagatacg ccctggttcc tggaacaatt
      121 gcttttacag atgcacatat cgaggtggac atcacttacg ctgagtactt cgaaatgtcc
      181 gttcggttgg cagaagctat gaaacgatat gggctgaata caaatcacag aatcgtcgta
      241 tgcagtgaaa actctcttca attctttatg ccggtgttgg gcgcgttatt tatcggagtt
      301 gcagttgcgc ccgcgaacga catttataat gaacgtgaat tgctcaacag tatgggcatt
      361 tcgcagccta ccgtggtgtt cgtttccaaa aaggggttgc aaaaaatttt gaacgtgcaa
      421 aaaaagctcc caatcatcca aaaaattatt atcatggatt ctaaaacgga ttaccaggga
      481 tttcagtcga tgtacacgtt cgtcacatct catctacctc ccggttttaa tgaatacgat
      541 tttgtgccag agtccttcga tagggacaag acaattgcac tgatcatgaa ctcctctgga
      601 tctactggtc tgcctaaagg tgtcgctctg cctcatagaa ctgcctgcgt gagattctcg
      661 catgccagag atcctatttt tggcaatcaa atcattccgg atactgcgat tttaagtgtt
      721 gttccattcc atcacggttt tggaatgttt actacactcg gatatttgat atgtggattt
      781 cgagtcgtct taatgtatag atttgaagaa gagctgtttc tgaggagcct tcaggattac
      841 aagattcaaa gtgcgctgct ggtgccaacc ctattctcct tcttcgccaa aagcactctg
      901 attgacaaat acgatttatc taatttacac gaaattgctt ctggtggcgc tcccctctct
      961 aaggaagtcg gggaagcggt tgccaagagg ttccatctgc caggtatcag gcaaggatat
     1021 gggctcactg agactacatc agctattctg attacacccg agggggatga taaaccgggc
     1081 gcggtcggta aagttgttcc attttttgaa gcgaaggttg tggatctgga taccgggaaa
     1141 acgctgggcg ttaatcaaag aggcgaactg tgtgtgagag gtcctatgat tatgtccggt
     1201 tatgtaaaca atccggaagc gaccaacgcc ttgattgaca aggatggatg gctacattct
     1261 ggagacatag cttactggga cgaagacgaa cacttcttca tcgttgaccg cctgaagtct
     1321 ctgattaagt acaaaggcta tcaggtggct cccgctgaat tggaatccat cttgctccaa
     1381 caccccaaca tcttcgacgc aggtgtcgca ggtcttcccg acgatgacgc cggtgaactt
     1441 cccgccgccg ttgttgtttt ggagcacgga aagacgatga cggaaaaaga gatcgtggat
     1501 tacgtcgcca gtcaagtaac aaccgcgaaa aagttgcgcg gaggagttgt gtttgtggac
     1561 gaagtaccga aaggtcttac cggaaaactc gacgcaagaa aaatcagaga gatcctcata
     1621 aaggccaaga agggcggaaa gatcgccgtg taatactaga gccaggcatc aaataaaacg
     1681 aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct
     1741 ctactagagt cacactggct caccttcggg tgggcctttc tgcgtttata tactagaggc
     1801 tttacaaaac tctcattaat cctttagact aagtttagtc agttccaatc tgaacatcga
     1861 caaatacata aggaattata accaatacta gatggcttcc aaggtgtacg accccgagca
     1921 acgcaaacgc atgatcactg ggcctcagtg gtgggctcgc tgcaagcaaa tgaacgtgct
     1981 ggactccttc atcaactact atgattccga gaagcacgcc gagaacgccg tgatttttct
     2041 gcatggtaac gctgcctcca gctacctgtg gaggcacgtc gtgcctcaca tcgagcccgt
     2101 ggctagatgc atcatccctg atctgatcgg aatgggtaag tccggcaaga gcgggaatgg
     2161 ctcatatcgc ctcctggatc actacaagta cctcaccgct tggttcgagc tgctgaacct
     2221 tccaaagaaa atcatctttg tgggccacga ctggggggct tgtctggcct ttcactactc
     2281 ctacgagcac caagacaaga tcaaggccat cgtccatgct gagagtgtcg tggacgtgat
     2341 cgagtcctgg gacgagtggc ctgacatcga ggaggatatc gccctgatca agagcgaaga
     2401 gggcgagaaa atggtgcttg agaataactt cttcgtcgag accatgctcc caagcaagat
     2461 catgcggaaa ctggagcctg aggagttcgc tgcctacctg gagccattca aggagaaggg
     2521 cgaggttaga cggcctaccc tctcctggcc tcgcgagatc cctctcgtta agggaggcaa
     2581 gcccgacgtc gtccagattg tccgcaacta caacgcctac cttcgggcca gcgacgatct
     2641 gcctaagatg ttcatcgagt ccgaccctgg gttcttttcc aacgctattg tcgagggagc
     2701 taagaagttc cctaacaccg agttcgtgaa ggtgaagggc ctccacttca gccaggagga
     2761 cgctccagat gaaatgggta agtacatcaa gagcttcgtg gagcgcgtgc tgaagaacga
     2821 gcagtaatac tagagccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct
     2881 ttcgttttat ctgttgtttg tcggtgaacg ctctctacta gagtcacact ggctcacctt
     2941 cgggtgggcc tttctgcgtt tata
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