LOCUS BBa_K654051 2993 bp DNA linear 27-Dec-2024 DEFINITION Promoter Tester 6803 Native RBS sigA Reference Dual Luciferase Assay ACCESSION BBa_K654051 VERSION BBa_K654051.1 FEATURES Location/Qualifiers misc_feature 1..49 /label=5' UTR with native RBS RBS 35..38 /label=SDS core consensus RBS 33..42 /label=native RBS RBS 1..49 /label=BBa_K390001 CDS 56..1708 /label=BBa_I712019 stem_loop 1728..1771 /label=stem_loop misc_feature 1717..1796 /label=BBa_B0010 terminator 1717..1796 /label=BBa_B0010 stem_loop 1812..1831 /label=T7 TE misc_feature 1838..1838 /label=stop polyA_site 1832..1845 /label=polya misc_feature 1805..1845 /label=BBa_B0012 terminator 1805..1845 /label=BBa_B0012 terminator 1717..1845 /label=BBa_B0015 misc_feature 56..1845 /label=BBa_K654033 misc_feature 1..1845 /label=BBa_K654043 promoter 1857..1898 /label=sigA promoter RBS 1899..1908 /label=putative rbs misc_feature 1899..1899 /label=+1 Transcription Start Point misc_feature 1862..1867 /label=-35 box misc_feature 1883..1888 /label=-10 box promoter 1854..1914 /label=BBa_K390018 misc_feature 1921..2856 /label=Rluc CDS 1921..2856 /label=Rluc misc_feature 1921..2856 /label=BBa_J52008 stem_loop 2876..2919 /label=stem_loop misc_feature 2865..2944 /label=BBa_B0010 terminator 2865..2944 /label=BBa_B0010 stem_loop 2960..2979 /label=T7 TE misc_feature 2986..2986 /label=stop polyA_site 2980..2993 /label=polya misc_feature 2953..2993 /label=BBa_B0012 terminator 2953..2993 /label=BBa_B0012 terminator 2865..2993 /label=BBa_B0015 misc_feature 1921..2993 /label=BBa_K654034 misc_feature 1854..2993 /label=BBa_K654038 ORIGIN 1 agtcagttcc aatctgaaca tcgacaaata cataaggaat tataaccaat actagatgga 61 agacgccaaa aacataaaga aaggcccggc gccattctat ccgctggaag atggaaccgc 121 tggagagcaa ctgcataagg ctatgaagag atacgccctg gttcctggaa caattgcttt 181 tacagatgca catatcgagg tggacatcac ttacgctgag tacttcgaaa tgtccgttcg 241 gttggcagaa gctatgaaac gatatgggct gaatacaaat cacagaatcg tcgtatgcag 301 tgaaaactct cttcaattct ttatgccggt gttgggcgcg ttatttatcg gagttgcagt 361 tgcgcccgcg aacgacattt ataatgaacg tgaattgctc aacagtatgg gcatttcgca 421 gcctaccgtg gtgttcgttt ccaaaaaggg gttgcaaaaa attttgaacg tgcaaaaaaa 481 gctcccaatc atccaaaaaa ttattatcat ggattctaaa acggattacc agggatttca 541 gtcgatgtac acgttcgtca catctcatct acctcccggt tttaatgaat acgattttgt 601 gccagagtcc ttcgataggg acaagacaat tgcactgatc atgaactcct ctggatctac 661 tggtctgcct aaaggtgtcg ctctgcctca tagaactgcc tgcgtgagat tctcgcatgc 721 cagagatcct atttttggca atcaaatcat tccggatact gcgattttaa gtgttgttcc 781 attccatcac ggttttggaa tgtttactac actcggatat ttgatatgtg gatttcgagt 841 cgtcttaatg tatagatttg aagaagagct gtttctgagg agccttcagg attacaagat 901 tcaaagtgcg ctgctggtgc caaccctatt ctccttcttc gccaaaagca ctctgattga 961 caaatacgat ttatctaatt tacacgaaat tgcttctggt ggcgctcccc tctctaagga 1021 agtcggggaa gcggttgcca agaggttcca tctgccaggt atcaggcaag gatatgggct 1081 cactgagact acatcagcta ttctgattac acccgagggg gatgataaac cgggcgcggt 1141 cggtaaagtt gttccatttt ttgaagcgaa ggttgtggat ctggataccg ggaaaacgct 1201 gggcgttaat caaagaggcg aactgtgtgt gagaggtcct atgattatgt ccggttatgt 1261 aaacaatccg gaagcgacca acgccttgat tgacaaggat ggatggctac attctggaga 1321 catagcttac tgggacgaag acgaacactt cttcatcgtt gaccgcctga agtctctgat 1381 taagtacaaa ggctatcagg tggctcccgc tgaattggaa tccatcttgc tccaacaccc 1441 caacatcttc gacgcaggtg tcgcaggtct tcccgacgat gacgccggtg aacttcccgc 1501 cgccgttgtt gttttggagc acggaaagac gatgacggaa aaagagatcg tggattacgt 1561 cgccagtcaa gtaacaaccg cgaaaaagtt gcgcggagga gttgtgtttg tggacgaagt 1621 accgaaaggt cttaccggaa aactcgacgc aagaaaaatc agagagatcc tcataaaggc 1681 caagaagggc ggaaagatcg ccgtgtaata ctagagccag gcatcaaata aaacgaaagg 1741 ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact 1801 agagtcacac tggctcacct tcgggtgggc ctttctgcgt ttatatacta gagcctcaac 1861 tttgactgac cagctaattt tgtacacgac ttaggagtta acgaggaaaa cggctactag 1921 atggcttcca aggtgtacga ccccgagcaa cgcaaacgca tgatcactgg gcctcagtgg 1981 tgggctcgct gcaagcaaat gaacgtgctg gactccttca tcaactacta tgattccgag 2041 aagcacgccg agaacgccgt gatttttctg catggtaacg ctgcctccag ctacctgtgg 2101 aggcacgtcg tgcctcacat cgagcccgtg gctagatgca tcatccctga tctgatcgga 2161 atgggtaagt ccggcaagag cgggaatggc tcatatcgcc tcctggatca ctacaagtac 2221 ctcaccgctt ggttcgagct gctgaacctt ccaaagaaaa tcatctttgt gggccacgac 2281 tggggggctt gtctggcctt tcactactcc tacgagcacc aagacaagat caaggccatc 2341 gtccatgctg agagtgtcgt ggacgtgatc gagtcctggg acgagtggcc tgacatcgag 2401 gaggatatcg ccctgatcaa gagcgaagag ggcgagaaaa tggtgcttga gaataacttc 2461 ttcgtcgaga ccatgctccc aagcaagatc atgcggaaac tggagcctga ggagttcgct 2521 gcctacctgg agccattcaa ggagaagggc gaggttagac ggcctaccct ctcctggcct 2581 cgcgagatcc ctctcgttaa gggaggcaag cccgacgtcg tccagattgt ccgcaactac 2641 aacgcctacc ttcgggccag cgacgatctg cctaagatgt tcatcgagtc cgaccctggg 2701 ttcttttcca acgctattgt cgagggagct aagaagttcc ctaacaccga gttcgtgaag 2761 gtgaagggcc tccacttcag ccaggaggac gctccagatg aaatgggtaa gtacatcaag 2821 agcttcgtgg agcgcgtgct gaagaacgag cagtaatact agagccaggc atcaaataaa 2881 acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc 2941 tctctactag agtcacactg gctcaccttc gggtgggcct ttctgcgttt ata //