LOCUS       BBa_K654052             2938 bp    DNA     linear       22-Nov-2024
DEFINITION  Promoter/RBS Tester sigA Reference Dual Luciferase Assay
ACCESSION   BBa_K654052
VERSION     BBa_K654052.1  
FEATURES             Location/Qualifiers
     CDS             1..1653
                     /label=BBa_I712019
     stem_loop       1673..1716
                     /label=stem_loop
     misc_feature    1662..1741
                     /label=BBa_B0010
     terminator      1662..1741
                     /label=BBa_B0010
     stem_loop       1757..1776
                     /label=T7 TE
     misc_feature    1783..1783
                     /label=stop
     polyA_site      1777..1790
                     /label=polya
     misc_feature    1750..1790
                     /label=BBa_B0012
     terminator      1750..1790
                     /label=BBa_B0012
     terminator      1662..1790
                     /label=BBa_B0015
     misc_feature    1..1790
                     /label=BBa_K654033
     promoter        1802..1843
                     /label=sigA promoter
     RBS             1844..1853
                     /label=putative rbs
     misc_feature    1844..1844
                     /label=+1 Transcription Start Point
     misc_feature    1807..1812
                     /label=-35 box
     misc_feature    1828..1833
                     /label=-10 box
     promoter        1799..1859
                     /label=BBa_K390018
     misc_feature    1866..2801
                     /label=Rluc
     CDS             1866..2801
                     /label=Rluc
     misc_feature    1866..2801
                     /label=BBa_J52008
     stem_loop       2821..2864
                     /label=stem_loop
     misc_feature    2810..2889
                     /label=BBa_B0010
     terminator      2810..2889
                     /label=BBa_B0010
     stem_loop       2905..2924
                     /label=T7 TE
     misc_feature    2931..2931
                     /label=stop
     polyA_site      2925..2938
                     /label=polya
     misc_feature    2898..2938
                     /label=BBa_B0012
     terminator      2898..2938
                     /label=BBa_B0012
     terminator      2810..2938
                     /label=BBa_B0015
     misc_feature    1866..2938
                     /label=BBa_K654034
     misc_feature    1799..2938
                     /label=BBa_K654038
ORIGIN
        1 atggaagacg ccaaaaacat aaagaaaggc ccggcgccat tctatccgct ggaagatgga
       61 accgctggag agcaactgca taaggctatg aagagatacg ccctggttcc tggaacaatt
      121 gcttttacag atgcacatat cgaggtggac atcacttacg ctgagtactt cgaaatgtcc
      181 gttcggttgg cagaagctat gaaacgatat gggctgaata caaatcacag aatcgtcgta
      241 tgcagtgaaa actctcttca attctttatg ccggtgttgg gcgcgttatt tatcggagtt
      301 gcagttgcgc ccgcgaacga catttataat gaacgtgaat tgctcaacag tatgggcatt
      361 tcgcagccta ccgtggtgtt cgtttccaaa aaggggttgc aaaaaatttt gaacgtgcaa
      421 aaaaagctcc caatcatcca aaaaattatt atcatggatt ctaaaacgga ttaccaggga
      481 tttcagtcga tgtacacgtt cgtcacatct catctacctc ccggttttaa tgaatacgat
      541 tttgtgccag agtccttcga tagggacaag acaattgcac tgatcatgaa ctcctctgga
      601 tctactggtc tgcctaaagg tgtcgctctg cctcatagaa ctgcctgcgt gagattctcg
      661 catgccagag atcctatttt tggcaatcaa atcattccgg atactgcgat tttaagtgtt
      721 gttccattcc atcacggttt tggaatgttt actacactcg gatatttgat atgtggattt
      781 cgagtcgtct taatgtatag atttgaagaa gagctgtttc tgaggagcct tcaggattac
      841 aagattcaaa gtgcgctgct ggtgccaacc ctattctcct tcttcgccaa aagcactctg
      901 attgacaaat acgatttatc taatttacac gaaattgctt ctggtggcgc tcccctctct
      961 aaggaagtcg gggaagcggt tgccaagagg ttccatctgc caggtatcag gcaaggatat
     1021 gggctcactg agactacatc agctattctg attacacccg agggggatga taaaccgggc
     1081 gcggtcggta aagttgttcc attttttgaa gcgaaggttg tggatctgga taccgggaaa
     1141 acgctgggcg ttaatcaaag aggcgaactg tgtgtgagag gtcctatgat tatgtccggt
     1201 tatgtaaaca atccggaagc gaccaacgcc ttgattgaca aggatggatg gctacattct
     1261 ggagacatag cttactggga cgaagacgaa cacttcttca tcgttgaccg cctgaagtct
     1321 ctgattaagt acaaaggcta tcaggtggct cccgctgaat tggaatccat cttgctccaa
     1381 caccccaaca tcttcgacgc aggtgtcgca ggtcttcccg acgatgacgc cggtgaactt
     1441 cccgccgccg ttgttgtttt ggagcacgga aagacgatga cggaaaaaga gatcgtggat
     1501 tacgtcgcca gtcaagtaac aaccgcgaaa aagttgcgcg gaggagttgt gtttgtggac
     1561 gaagtaccga aaggtcttac cggaaaactc gacgcaagaa aaatcagaga gatcctcata
     1621 aaggccaaga agggcggaaa gatcgccgtg taatactaga gccaggcatc aaataaaacg
     1681 aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct
     1741 ctactagagt cacactggct caccttcggg tgggcctttc tgcgtttata tactagagcc
     1801 tcaactttga ctgaccagct aattttgtac acgacttagg agttaacgag gaaaacggct
     1861 actagatggc ttccaaggtg tacgaccccg agcaacgcaa acgcatgatc actgggcctc
     1921 agtggtgggc tcgctgcaag caaatgaacg tgctggactc cttcatcaac tactatgatt
     1981 ccgagaagca cgccgagaac gccgtgattt ttctgcatgg taacgctgcc tccagctacc
     2041 tgtggaggca cgtcgtgcct cacatcgagc ccgtggctag atgcatcatc cctgatctga
     2101 tcggaatggg taagtccggc aagagcggga atggctcata tcgcctcctg gatcactaca
     2161 agtacctcac cgcttggttc gagctgctga accttccaaa gaaaatcatc tttgtgggcc
     2221 acgactgggg ggcttgtctg gcctttcact actcctacga gcaccaagac aagatcaagg
     2281 ccatcgtcca tgctgagagt gtcgtggacg tgatcgagtc ctgggacgag tggcctgaca
     2341 tcgaggagga tatcgccctg atcaagagcg aagagggcga gaaaatggtg cttgagaata
     2401 acttcttcgt cgagaccatg ctcccaagca agatcatgcg gaaactggag cctgaggagt
     2461 tcgctgccta cctggagcca ttcaaggaga agggcgaggt tagacggcct accctctcct
     2521 ggcctcgcga gatccctctc gttaagggag gcaagcccga cgtcgtccag attgtccgca
     2581 actacaacgc ctaccttcgg gccagcgacg atctgcctaa gatgttcatc gagtccgacc
     2641 ctgggttctt ttccaacgct attgtcgagg gagctaagaa gttccctaac accgagttcg
     2701 tgaaggtgaa gggcctccac ttcagccagg aggacgctcc agatgaaatg ggtaagtaca
     2761 tcaagagctt cgtggagcgc gtgctgaaga acgagcagta atactagagc caggcatcaa
     2821 ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg
     2881 aacgctctct actagagtca cactggctca ccttcgggtg ggcctttctg cgtttata
//