LOCUS BBa_K654053 3005 bp DNA linear 15-Nov-2024 DEFINITION Promoter Control sigA Reference Dual Luciferase Assay ACCESSION BBa_K654053 VERSION BBa_K654053.1 FEATURES Location/Qualifiers promoter 4..45 /label=sigA promoter RBS 46..55 /label=putative rbs misc_feature 46..46 /label=+1 Transcription Start Point misc_feature 9..14 /label=-35 box misc_feature 30..35 /label=-10 box promoter 1..61 /label=BBa_K390018 CDS 68..1720 /label=BBa_I712019 stem_loop 1740..1783 /label=stem_loop misc_feature 1729..1808 /label=BBa_B0010 terminator 1729..1808 /label=BBa_B0010 stem_loop 1824..1843 /label=T7 TE misc_feature 1850..1850 /label=stop polyA_site 1844..1857 /label=polya misc_feature 1817..1857 /label=BBa_B0012 terminator 1817..1857 /label=BBa_B0012 terminator 1729..1857 /label=BBa_B0015 misc_feature 68..1857 /label=BBa_K654033 misc_feature 1..1857 /label=BBa_K654041 promoter 1869..1910 /label=sigA promoter RBS 1911..1920 /label=putative rbs misc_feature 1911..1911 /label=+1 Transcription Start Point misc_feature 1874..1879 /label=-35 box misc_feature 1895..1900 /label=-10 box promoter 1866..1926 /label=BBa_K390018 misc_feature 1933..2868 /label=Rluc CDS 1933..2868 /label=Rluc misc_feature 1933..2868 /label=BBa_J52008 stem_loop 2888..2931 /label=stem_loop misc_feature 2877..2956 /label=BBa_B0010 terminator 2877..2956 /label=BBa_B0010 stem_loop 2972..2991 /label=T7 TE misc_feature 2998..2998 /label=stop polyA_site 2992..3005 /label=polya misc_feature 2965..3005 /label=BBa_B0012 terminator 2965..3005 /label=BBa_B0012 terminator 2877..3005 /label=BBa_B0015 misc_feature 1933..3005 /label=BBa_K654034 misc_feature 1866..3005 /label=BBa_K654038 ORIGIN 1 cctcaacttt gactgaccag ctaattttgt acacgactta ggagttaacg aggaaaacgg 61 ctactagatg gaagacgcca aaaacataaa gaaaggcccg gcgccattct atccgctgga 121 agatggaacc gctggagagc aactgcataa ggctatgaag agatacgccc tggttcctgg 181 aacaattgct tttacagatg cacatatcga ggtggacatc acttacgctg agtacttcga 241 aatgtccgtt cggttggcag aagctatgaa acgatatggg ctgaatacaa atcacagaat 301 cgtcgtatgc agtgaaaact ctcttcaatt ctttatgccg gtgttgggcg cgttatttat 361 cggagttgca gttgcgcccg cgaacgacat ttataatgaa cgtgaattgc tcaacagtat 421 gggcatttcg cagcctaccg tggtgttcgt ttccaaaaag gggttgcaaa aaattttgaa 481 cgtgcaaaaa aagctcccaa tcatccaaaa aattattatc atggattcta aaacggatta 541 ccagggattt cagtcgatgt acacgttcgt cacatctcat ctacctcccg gttttaatga 601 atacgatttt gtgccagagt ccttcgatag ggacaagaca attgcactga tcatgaactc 661 ctctggatct actggtctgc ctaaaggtgt cgctctgcct catagaactg cctgcgtgag 721 attctcgcat gccagagatc ctatttttgg caatcaaatc attccggata ctgcgatttt 781 aagtgttgtt ccattccatc acggttttgg aatgtttact acactcggat atttgatatg 841 tggatttcga gtcgtcttaa tgtatagatt tgaagaagag ctgtttctga ggagccttca 901 ggattacaag attcaaagtg cgctgctggt gccaacccta ttctccttct tcgccaaaag 961 cactctgatt gacaaatacg atttatctaa tttacacgaa attgcttctg gtggcgctcc 1021 cctctctaag gaagtcgggg aagcggttgc caagaggttc catctgccag gtatcaggca 1081 aggatatggg ctcactgaga ctacatcagc tattctgatt acacccgagg gggatgataa 1141 accgggcgcg gtcggtaaag ttgttccatt ttttgaagcg aaggttgtgg atctggatac 1201 cgggaaaacg ctgggcgtta atcaaagagg cgaactgtgt gtgagaggtc ctatgattat 1261 gtccggttat gtaaacaatc cggaagcgac caacgccttg attgacaagg atggatggct 1321 acattctgga gacatagctt actgggacga agacgaacac ttcttcatcg ttgaccgcct 1381 gaagtctctg attaagtaca aaggctatca ggtggctccc gctgaattgg aatccatctt 1441 gctccaacac cccaacatct tcgacgcagg tgtcgcaggt cttcccgacg atgacgccgg 1501 tgaacttccc gccgccgttg ttgttttgga gcacggaaag acgatgacgg aaaaagagat 1561 cgtggattac gtcgccagtc aagtaacaac cgcgaaaaag ttgcgcggag gagttgtgtt 1621 tgtggacgaa gtaccgaaag gtcttaccgg aaaactcgac gcaagaaaaa tcagagagat 1681 cctcataaag gccaagaagg gcggaaagat cgccgtgtaa tactagagcc aggcatcaaa 1741 taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga 1801 acgctctcta ctagagtcac actggctcac cttcgggtgg gcctttctgc gtttatatac 1861 tagagcctca actttgactg accagctaat tttgtacacg acttaggagt taacgaggaa 1921 aacggctact agatggcttc caaggtgtac gaccccgagc aacgcaaacg catgatcact 1981 gggcctcagt ggtgggctcg ctgcaagcaa atgaacgtgc tggactcctt catcaactac 2041 tatgattccg agaagcacgc cgagaacgcc gtgatttttc tgcatggtaa cgctgcctcc 2101 agctacctgt ggaggcacgt cgtgcctcac atcgagcccg tggctagatg catcatccct 2161 gatctgatcg gaatgggtaa gtccggcaag agcgggaatg gctcatatcg cctcctggat 2221 cactacaagt acctcaccgc ttggttcgag ctgctgaacc ttccaaagaa aatcatcttt 2281 gtgggccacg actggggggc ttgtctggcc tttcactact cctacgagca ccaagacaag 2341 atcaaggcca tcgtccatgc tgagagtgtc gtggacgtga tcgagtcctg ggacgagtgg 2401 cctgacatcg aggaggatat cgccctgatc aagagcgaag agggcgagaa aatggtgctt 2461 gagaataact tcttcgtcga gaccatgctc ccaagcaaga tcatgcggaa actggagcct 2521 gaggagttcg ctgcctacct ggagccattc aaggagaagg gcgaggttag acggcctacc 2581 ctctcctggc ctcgcgagat ccctctcgtt aagggaggca agcccgacgt cgtccagatt 2641 gtccgcaact acaacgccta ccttcgggcc agcgacgatc tgcctaagat gttcatcgag 2701 tccgaccctg ggttcttttc caacgctatt gtcgagggag ctaagaagtt ccctaacacc 2761 gagttcgtga aggtgaaggg cctccacttc agccaggagg acgctccaga tgaaatgggt 2821 aagtacatca agagcttcgt ggagcgcgtg ctgaagaacg agcagtaata ctagagccag 2881 gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt 2941 gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc ctttctgcgt 3001 ttata //