##gff-version 3
##sequence-region BBa_K654053 1 3005
BBa_K654053	.	engineered_region	1866	3005	.	+	0	ID=BBa_K654038;Name=BBa_K654038
BBa_K654053	.	engineered_region	1933	3005	.	+	0	ID=BBa_K654034;Name=BBa_K654034;Parent=BBa_K654038
BBa_K654053	.	engineered_region	1933	2868	.	+	0	ID=BBa_J52008;Name=BBa_J52008;Parent=BBa_K654034
BBa_K654053	.	CDS	1933	2868	.	+	0	ID=annotation1902845;Name=Rluc;Parent=BBa_J52008
BBa_K654053	.	engineered_region	1933	2868	.	+	0	ID=annotation1902833;Name=Rluc;Parent=BBa_J52008
BBa_K654053	.	terminator	2877	3005	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K654034
BBa_K654053	.	terminator	2965	3005	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K654053	.	engineered_region	2965	3005	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K654053	.	stem_loop	2972	2991	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K654053	.	stop_codon	2998	2998	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K654053	.	polyA_site	2992	3005	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K654053	.	terminator	2877	2956	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K654053	.	engineered_region	2877	2956	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K654053	.	stem_loop	2888	2931	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K654053	.	promoter	1866	1926	.	+	0	ID=BBa_K390018;Name=BBa_K390018;Parent=BBa_K654038
BBa_K654053	.	sequence_feature	1874	1879	.	+	0	ID=annotation2076611;Name=-35 box;Parent=BBa_K390018
BBa_K654053	.	ribosome_entry_site	1911	1920	.	+	0	ID=annotation2076609;Name=putative rbs;Parent=BBa_K390018
BBa_K654053	.	sequence_feature	1895	1900	.	+	0	ID=annotation2076612;Name=-10 box;Parent=BBa_K390018
BBa_K654053	.	sequence_feature	1911	1911	.	+	0	ID=annotation2076610;Name=+1 Transcription Start Point;Parent=BBa_K390018
BBa_K654053	.	promoter	1869	1910	.	+	0	ID=annotation2076608;Name=sigA promoter;Parent=BBa_K390018
BBa_K654053	.	engineered_region	1	1857	.	+	0	ID=BBa_K654041;Name=BBa_K654041
BBa_K654053	.	promoter	1	61	.	+	0	ID=BBa_K390018;Name=BBa_K390018;Parent=BBa_K654041
BBa_K654053	.	sequence_feature	9	14	.	+	0	ID=annotation2076611;Name=-35 box;Parent=BBa_K390018
BBa_K654053	.	ribosome_entry_site	46	55	.	+	0	ID=annotation2076609;Name=putative rbs;Parent=BBa_K390018
BBa_K654053	.	sequence_feature	30	35	.	+	0	ID=annotation2076612;Name=-10 box;Parent=BBa_K390018
BBa_K654053	.	sequence_feature	46	46	.	+	0	ID=annotation2076610;Name=+1 Transcription Start Point;Parent=BBa_K390018
BBa_K654053	.	promoter	4	45	.	+	0	ID=annotation2076608;Name=sigA promoter;Parent=BBa_K390018
BBa_K654053	.	engineered_region	68	1857	.	+	0	ID=BBa_K654033;Name=BBa_K654033;Parent=BBa_K654041
BBa_K654053	.	terminator	1729	1857	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K654033
BBa_K654053	.	terminator	1817	1857	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K654053	.	engineered_region	1817	1857	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K654053	.	stem_loop	1824	1843	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K654053	.	stop_codon	1850	1850	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K654053	.	polyA_site	1844	1857	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K654053	.	terminator	1729	1808	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K654053	.	engineered_region	1729	1808	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K654053	.	stem_loop	1740	1783	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K654053	.	CDS	68	1720	.	+	0	ID=BBa_I712019;Name=BBa_I712019;Parent=BBa_K654033
>BBa_K654053
cctcaactttgactgaccagctaattttgtacacgacttaggagttaacgaggaaaacggctactagatggaagacgcc
aaaaacataaagaaaggcccggcgccattctatccgctggaagatggaaccgctggagagcaactgcataaggctatga
agagatacgccctggttcctggaacaattgcttttacagatgcacatatcgaggtggacatcacttacgctgagtactt
cgaaatgtccgttcggttggcagaagctatgaaacgatatgggctgaatacaaatcacagaatcgtcgtatgcagtgaa
aactctcttcaattctttatgccggtgttgggcgcgttatttatcggagttgcagttgcgcccgcgaacgacatttata
atgaacgtgaattgctcaacagtatgggcatttcgcagcctaccgtggtgttcgtttccaaaaaggggttgcaaaaaat
tttgaacgtgcaaaaaaagctcccaatcatccaaaaaattattatcatggattctaaaacggattaccagggatttcag
tcgatgtacacgttcgtcacatctcatctacctcccggttttaatgaatacgattttgtgccagagtccttcgataggg
acaagacaattgcactgatcatgaactcctctggatctactggtctgcctaaaggtgtcgctctgcctcatagaactgc
ctgcgtgagattctcgcatgccagagatcctatttttggcaatcaaatcattccggatactgcgattttaagtgttgtt
ccattccatcacggttttggaatgtttactacactcggatatttgatatgtggatttcgagtcgtcttaatgtatagat
ttgaagaagagctgtttctgaggagccttcaggattacaagattcaaagtgcgctgctggtgccaaccctattctcctt
cttcgccaaaagcactctgattgacaaatacgatttatctaatttacacgaaattgcttctggtggcgctcccctctct
aaggaagtcggggaagcggttgccaagaggttccatctgccaggtatcaggcaaggatatgggctcactgagactacat
cagctattctgattacacccgagggggatgataaaccgggcgcggtcggtaaagttgttccattttttgaagcgaaggt
tgtggatctggataccgggaaaacgctgggcgttaatcaaagaggcgaactgtgtgtgagaggtcctatgattatgtcc
ggttatgtaaacaatccggaagcgaccaacgccttgattgacaaggatggatggctacattctggagacatagcttact
gggacgaagacgaacacttcttcatcgttgaccgcctgaagtctctgattaagtacaaaggctatcaggtggctcccgc
tgaattggaatccatcttgctccaacaccccaacatcttcgacgcaggtgtcgcaggtcttcccgacgatgacgccggt
gaacttcccgccgccgttgttgttttggagcacggaaagacgatgacggaaaaagagatcgtggattacgtcgccagtc
aagtaacaaccgcgaaaaagttgcgcggaggagttgtgtttgtggacgaagtaccgaaaggtcttaccggaaaactcga
cgcaagaaaaatcagagagatcctcataaaggccaagaagggcggaaagatcgccgtgtaatactagagccaggcatca
aataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagt
cacactggctcaccttcgggtgggcctttctgcgtttatatactagagcctcaactttgactgaccagctaattttgta
cacgacttaggagttaacgaggaaaacggctactagatggcttccaaggtgtacgaccccgagcaacgcaaacgcatga
tcactgggcctcagtggtgggctcgctgcaagcaaatgaacgtgctggactccttcatcaactactatgattccgagaa
gcacgccgagaacgccgtgatttttctgcatggtaacgctgcctccagctacctgtggaggcacgtcgtgcctcacatc
gagcccgtggctagatgcatcatccctgatctgatcggaatgggtaagtccggcaagagcgggaatggctcatatcgcc
tcctggatcactacaagtacctcaccgcttggttcgagctgctgaaccttccaaagaaaatcatctttgtgggccacga
ctggggggcttgtctggcctttcactactcctacgagcaccaagacaagatcaaggccatcgtccatgctgagagtgtc
gtggacgtgatcgagtcctgggacgagtggcctgacatcgaggaggatatcgccctgatcaagagcgaagagggcgaga
aaatggtgcttgagaataacttcttcgtcgagaccatgctcccaagcaagatcatgcggaaactggagcctgaggagtt
cgctgcctacctggagccattcaaggagaagggcgaggttagacggcctaccctctcctggcctcgcgagatccctctc
gttaagggaggcaagcccgacgtcgtccagattgtccgcaactacaacgcctaccttcgggccagcgacgatctgccta
agatgttcatcgagtccgaccctgggttcttttccaacgctattgtcgagggagctaagaagttccctaacaccgagtt
cgtgaaggtgaagggcctccacttcagccaggaggacgctccagatgaaatgggtaagtacatcaagagcttcgtggag
cgcgtgctgaagaacgagcagtaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggccttt
cgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgttt
ata