LOCUS BBa_K654054 3019 bp DNA linear 26-Nov-2024 DEFINITION Promoter Tester 6803 Consensus RBS psbA2 Reference Dual Luciferase Assay ACCESSION BBa_K654054 VERSION BBa_K654054.1 FEATURES Location/Qualifiers misc_feature 1..49 /label=5' UTR with consensus RBS RBS 33..42 /label=consensus RBS RBS 35..40 /label=SDS core consensus misc_feature 33..33 /label=t > a misc_feature 39..40 /label=at > gg misc_feature 42..42 /label=a > t RBS 1..49 /label=BBa_K390002 CDS 56..1708 /label=BBa_I712019 stem_loop 1728..1771 /label=stem_loop misc_feature 1717..1796 /label=BBa_B0010 terminator 1717..1796 /label=BBa_B0010 stem_loop 1812..1831 /label=T7 TE misc_feature 1838..1838 /label=stop polyA_site 1832..1845 /label=polya misc_feature 1805..1845 /label=BBa_B0012 terminator 1805..1845 /label=BBa_B0012 terminator 1717..1845 /label=BBa_B0015 misc_feature 56..1845 /label=BBa_K654033 misc_feature 1..1845 /label=BBa_K654044 promoter 1854..1891 /label=psbA2 promoter RBS 1924..1933 /label=putative rbs misc_feature 1856..1861 /label=-35 box misc_feature 1880..1885 /label=-10 box misc_feature 1892..1892 /label=+1 Transcription Start Point promoter 1854..1940 /label=BBa_K390017 misc_feature 1947..2882 /label=Rluc CDS 1947..2882 /label=Rluc misc_feature 1947..2882 /label=BBa_J52008 stem_loop 2902..2945 /label=stem_loop misc_feature 2891..2970 /label=BBa_B0010 terminator 2891..2970 /label=BBa_B0010 stem_loop 2986..3005 /label=T7 TE misc_feature 3012..3012 /label=stop polyA_site 3006..3019 /label=polya misc_feature 2979..3019 /label=BBa_B0012 terminator 2979..3019 /label=BBa_B0012 terminator 2891..3019 /label=BBa_B0015 misc_feature 1947..3019 /label=BBa_K654034 misc_feature 1854..3019 /label=BBa_K654037 ORIGIN 1 agtcagttcc aatctgaaca tcgacaaata caaaaggagg tttaaccaat actagatgga 61 agacgccaaa aacataaaga aaggcccggc gccattctat ccgctggaag atggaaccgc 121 tggagagcaa ctgcataagg ctatgaagag atacgccctg gttcctggaa caattgcttt 181 tacagatgca catatcgagg tggacatcac ttacgctgag tacttcgaaa tgtccgttcg 241 gttggcagaa gctatgaaac gatatgggct gaatacaaat cacagaatcg tcgtatgcag 301 tgaaaactct cttcaattct ttatgccggt gttgggcgcg ttatttatcg gagttgcagt 361 tgcgcccgcg aacgacattt ataatgaacg tgaattgctc aacagtatgg gcatttcgca 421 gcctaccgtg gtgttcgttt ccaaaaaggg gttgcaaaaa attttgaacg tgcaaaaaaa 481 gctcccaatc atccaaaaaa ttattatcat ggattctaaa acggattacc agggatttca 541 gtcgatgtac acgttcgtca catctcatct acctcccggt tttaatgaat acgattttgt 601 gccagagtcc ttcgataggg acaagacaat tgcactgatc atgaactcct ctggatctac 661 tggtctgcct aaaggtgtcg ctctgcctca tagaactgcc tgcgtgagat tctcgcatgc 721 cagagatcct atttttggca atcaaatcat tccggatact gcgattttaa gtgttgttcc 781 attccatcac ggttttggaa tgtttactac actcggatat ttgatatgtg gatttcgagt 841 cgtcttaatg tatagatttg aagaagagct gtttctgagg agccttcagg attacaagat 901 tcaaagtgcg ctgctggtgc caaccctatt ctccttcttc gccaaaagca ctctgattga 961 caaatacgat ttatctaatt tacacgaaat tgcttctggt ggcgctcccc tctctaagga 1021 agtcggggaa gcggttgcca agaggttcca tctgccaggt atcaggcaag gatatgggct 1081 cactgagact acatcagcta ttctgattac acccgagggg gatgataaac cgggcgcggt 1141 cggtaaagtt gttccatttt ttgaagcgaa ggttgtggat ctggataccg ggaaaacgct 1201 gggcgttaat caaagaggcg aactgtgtgt gagaggtcct atgattatgt ccggttatgt 1261 aaacaatccg gaagcgacca acgccttgat tgacaaggat ggatggctac attctggaga 1321 catagcttac tgggacgaag acgaacactt cttcatcgtt gaccgcctga agtctctgat 1381 taagtacaaa ggctatcagg tggctcccgc tgaattggaa tccatcttgc tccaacaccc 1441 caacatcttc gacgcaggtg tcgcaggtct tcccgacgat gacgccggtg aacttcccgc 1501 cgccgttgtt gttttggagc acggaaagac gatgacggaa aaagagatcg tggattacgt 1561 cgccagtcaa gtaacaaccg cgaaaaagtt gcgcggagga gttgtgtttg tggacgaagt 1621 accgaaaggt cttaccggaa aactcgacgc aagaaaaatc agagagatcc tcataaaggc 1681 caagaagggc ggaaagatcg ccgtgtaata ctagagccag gcatcaaata aaacgaaagg 1741 ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact 1801 agagtcacac tggctcacct tcgggtgggc ctttctgcgt ttatatacta gaggctttac 1861 aaaactctca ttaatccttt agactaagtt tagtcagttc caatctgaac atcgacaaat 1921 acataaggaa ttataaccaa tactagatgg cttccaaggt gtacgacccc gagcaacgca 1981 aacgcatgat cactgggcct cagtggtggg ctcgctgcaa gcaaatgaac gtgctggact 2041 ccttcatcaa ctactatgat tccgagaagc acgccgagaa cgccgtgatt tttctgcatg 2101 gtaacgctgc ctccagctac ctgtggaggc acgtcgtgcc tcacatcgag cccgtggcta 2161 gatgcatcat ccctgatctg atcggaatgg gtaagtccgg caagagcggg aatggctcat 2221 atcgcctcct ggatcactac aagtacctca ccgcttggtt cgagctgctg aaccttccaa 2281 agaaaatcat ctttgtgggc cacgactggg gggcttgtct ggcctttcac tactcctacg 2341 agcaccaaga caagatcaag gccatcgtcc atgctgagag tgtcgtggac gtgatcgagt 2401 cctgggacga gtggcctgac atcgaggagg atatcgccct gatcaagagc gaagagggcg 2461 agaaaatggt gcttgagaat aacttcttcg tcgagaccat gctcccaagc aagatcatgc 2521 ggaaactgga gcctgaggag ttcgctgcct acctggagcc attcaaggag aagggcgagg 2581 ttagacggcc taccctctcc tggcctcgcg agatccctct cgttaaggga ggcaagcccg 2641 acgtcgtcca gattgtccgc aactacaacg cctaccttcg ggccagcgac gatctgccta 2701 agatgttcat cgagtccgac cctgggttct tttccaacgc tattgtcgag ggagctaaga 2761 agttccctaa caccgagttc gtgaaggtga agggcctcca cttcagccag gaggacgctc 2821 cagatgaaat gggtaagtac atcaagagct tcgtggagcg cgtgctgaag aacgagcagt 2881 aatactagag ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt 2941 tttatctgtt gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt 3001 gggcctttct gcgtttata //