BBa_K654054
type: ComponentDefinition
persistent identity: BBa K654054
display id: BBa_K654054
version: 1
was generated by: iGEM to SBOL conversion
description: Promoter Tester 6803 Consensus RBS psbA2 Reference Dual Luciferase Assay
created: 2011-09-25T11:00:00Z
modified: 2015-05-08T01:13:01Z
mutable provenance: existing parts
owned by: james
owned by: myers
mutable description: to be added
discontinued: false
dominant: false
experience: experience/None
group u list: _831_
m user id: 0
owner id: 10367
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: none
star: false
creator: Victor Carlson
type: DnaRegion
role: SO:0000804
role: partType/Measurement
component: BBa_K654037
component: BBa_K654044
sequence annotation: BBa_K654044
sequence annotation: BBa_K654037
sequence: https://synbiohub.org/public/igem/BBa_K654054_sequence/1

BBa_K654037
type: Component
persistent identity: component2142419
display id: component2142419
version: 1
top level: BBa_K654054
definition: BBa_K654037
access: public

BBa_K654044
type: Component
persistent identity: component2142401
display id: component2142401
version: 1
top level: BBa_K654054
definition: BBa_K654044
access: public

BBa_K654044
type: SequenceAnnotation
persistent identity: annotation2142401
display id: annotation2142401
version: 1
top level: BBa_K654054
location: https://synbiohub.org/public/igem/BBa_K654054/annotation2142401/range2142401/1
component: BBa_K654044

https://synbiohub.org/public/igem/BBa_K654054/annotation2142401/range2142401/1
type: Range
persistent identity: range2142401
display id: range2142401
version: 1
top level: BBa_K654054
direction: direction/reverse
start: 1
end: 1845
orientation: inline

BBa_K654037
type: SequenceAnnotation
persistent identity: annotation2142419
display id: annotation2142419
version: 1
top level: BBa_K654054
location: https://synbiohub.org/public/igem/BBa_K654054/annotation2142419/range2142419/1
component: BBa_K654037

https://synbiohub.org/public/igem/BBa_K654054/annotation2142419/range2142419/1
type: Range
persistent identity: range2142419
display id: range2142419
version: 1
top level: BBa_K654054
direction: direction/reverse
start: 1854
end: 3019
orientation: inline

BBa_K654037
type: ComponentDefinition
persistent identity: BBa K654037
display id: BBa_K654037
version: 1
was generated by: iGEM to SBOL conversion
description: Synechocystis psbA2 Promoter Controlled Renilla Luciferase
created: 2011-09-25T11:00:00Z
modified: 2015-05-08T01:13:01Z
mutable provenance: existing parts
owned by: james
owned by: myers
mutable description: to be added
discontinued: false
dominant: false
group u list: _831_
m user id: 0
owner id: 4660
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: none
star: false
creator: Cole Peterson
type: DnaRegion
role: partType/Generator
role: SO:0000804
component: BBa_K654034
component: BBa_K390017
sequence annotation: BBa_K390017
sequence annotation: BBa_K654034
sequence: https://synbiohub.org/public/igem/BBa_K654037_sequence/1

BBa_K654034
type: Component
persistent identity: component2142055
display id: component2142055
version: 1
top level: BBa_K654037
definition: BBa_K654034
access: public

BBa_K390017
type: Component
persistent identity: component2142044
display id: component2142044
version: 1
top level: BBa_K654037
definition: BBa_K390017
access: public

BBa_K390017
type: SequenceAnnotation
persistent identity: annotation2142044
display id: annotation2142044
version: 1
top level: BBa_K654037
location: https://synbiohub.org/public/igem/BBa_K654037/annotation2142044/range2142044/1
component: BBa_K390017

https://synbiohub.org/public/igem/BBa_K654037/annotation2142044/range2142044/1
type: Range
persistent identity: range2142044
display id: range2142044
version: 1
top level: BBa_K654037
direction: direction/reverse
start: 1
end: 87
orientation: inline

BBa_K654034
type: SequenceAnnotation
persistent identity: annotation2142055
display id: annotation2142055
version: 1
top level: BBa_K654037
location: https://synbiohub.org/public/igem/BBa_K654037/annotation2142055/range2142055/1
component: BBa_K654034

https://synbiohub.org/public/igem/BBa_K654037/annotation2142055/range2142055/1
type: Range
persistent identity: range2142055
display id: range2142055
version: 1
top level: BBa_K654037
direction: direction/reverse
start: 94
end: 1166
orientation: inline

BBa_K654044
type: ComponentDefinition
persistent identity: BBa K654044
display id: BBa_K654044
version: 1
was generated by: iGEM to SBOL conversion
description: Synechocystis psbA2 5'UTR Consensus RBS + Firefly Luciferase
created: 2011-09-25T11:00:00Z
modified: 2015-05-08T01:13:01Z
mutable provenance: existing parts
owned by: james
owned by: myers
mutable description: to be added
discontinued: false
dominant: false
group u list: _831_
m user id: 0
owner id: 10129
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: none
star: false
creator: Eric B Anderson
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_K654033
component: BBa_K390002
sequence annotation: BBa_K390002
sequence annotation: BBa_K654033
sequence: https://synbiohub.org/public/igem/BBa_K654044_sequence/1

BBa_K654033
type: Component
persistent identity: component2142193
display id: component2142193
version: 1
top level: BBa_K654044
definition: BBa_K654033
access: public

BBa_K390002
type: Component
persistent identity: component2142184
display id: component2142184
version: 1
top level: BBa_K654044
definition: BBa_K390002
access: public

BBa_K390002
type: SequenceAnnotation
persistent identity: annotation2142184
display id: annotation2142184
version: 1
top level: BBa_K654044
location: https://synbiohub.org/public/igem/BBa_K654044/annotation2142184/range2142184/1
component: BBa_K390002

https://synbiohub.org/public/igem/BBa_K654044/annotation2142184/range2142184/1
type: Range
persistent identity: range2142184
display id: range2142184
version: 1
top level: BBa_K654044
direction: direction/reverse
start: 1
end: 49
orientation: inline

BBa_K654033
type: SequenceAnnotation
persistent identity: annotation2142193
display id: annotation2142193
version: 1
top level: BBa_K654044
location: https://synbiohub.org/public/igem/BBa_K654044/annotation2142193/range2142193/1
component: BBa_K654033

https://synbiohub.org/public/igem/BBa_K654044/annotation2142193/range2142193/1
type: Range
persistent identity: range2142193
display id: range2142193
version: 1
top level: BBa_K654044
direction: direction/reverse
start: 56
end: 1845
orientation: inline

BBa_K654033
type: ComponentDefinition
persistent identity: BBa K654033
display id: BBa_K654033
version: 1
was generated by: iGEM to SBOL conversion
description: Firefly Luciferase + Terminator
created: 2011-09-25T11:00:00Z
modified: 2015-05-08T01:13:01Z
mutable provenance: existing parts
owned by: james
owned by: myers
mutable description: to be added
discontinued: false
dominant: false
experience: experience/None
group u list: _831_
m user id: 0
owner id: 4660
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: none
star: false
creator: Cole Peterson
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_B0015
component: BBa_I712019
sequence annotation: BBa_I712019
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_K654033_sequence/1

BBa_B0015
type: Component
persistent identity: component2141982
display id: component2141982
version: 1
top level: BBa_K654033
definition: BBa_B0015
access: public

BBa_I712019
type: Component
persistent identity: component2141975
display id: component2141975
version: 1
top level: BBa_K654033
definition: BBa_I712019
access: public

BBa_I712019
type: SequenceAnnotation
persistent identity: annotation2141975
display id: annotation2141975
version: 1
top level: BBa_K654033
location: https://synbiohub.org/public/igem/BBa_K654033/annotation2141975/range2141975/1
component: BBa_I712019

https://synbiohub.org/public/igem/BBa_K654033/annotation2141975/range2141975/1
type: Range
persistent identity: range2141975
display id: range2141975
version: 1
top level: BBa_K654033
direction: direction/reverse
start: 1
end: 1653
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2141982
display id: annotation2141982
version: 1
top level: BBa_K654033
location: https://synbiohub.org/public/igem/BBa_K654033/annotation2141982/range2141982/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K654033/annotation2141982/range2141982/1
type: Range
persistent identity: range2141982
display id: range2141982
version: 1
top level: BBa_K654033
direction: direction/reverse
start: 1662
end: 1790
orientation: inline

BBa_K654034
type: ComponentDefinition
persistent identity: BBa K654034
display id: BBa_K654034
version: 1
was generated by: iGEM to SBOL conversion
description: Renilla Luciferase + Terminator
created: 2011-09-25T11:00:00Z
modified: 2015-05-08T01:13:01Z
mutable provenance: existing parts
owned by: james
owned by: myers
mutable description: to be added
discontinued: false
dominant: false
group u list: _831_
m user id: 0
owner id: 4660
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: none
star: false
creator: Cole Peterson
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_B0015
component: BBa_J52008
sequence annotation: BBa_J52008
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_K654034_sequence/1

BBa_B0015
type: Component
persistent identity: component2141993
display id: component2141993
version: 1
top level: BBa_K654034
definition: BBa_B0015
access: public

BBa_J52008
type: Component
persistent identity: component2141986
display id: component2141986
version: 1
top level: BBa_K654034
definition: BBa_J52008
access: public

BBa_J52008
type: SequenceAnnotation
persistent identity: annotation2141986
display id: annotation2141986
version: 1
top level: BBa_K654034
location: https://synbiohub.org/public/igem/BBa_K654034/annotation2141986/range2141986/1
component: BBa_J52008

https://synbiohub.org/public/igem/BBa_K654034/annotation2141986/range2141986/1
type: Range
persistent identity: range2141986
display id: range2141986
version: 1
top level: BBa_K654034
direction: direction/reverse
start: 1
end: 936
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2141993
display id: annotation2141993
version: 1
top level: BBa_K654034
location: https://synbiohub.org/public/igem/BBa_K654034/annotation2141993/range2141993/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K654034/annotation2141993/range2141993/1
type: Range
persistent identity: range2141993
display id: range2141993
version: 1
top level: BBa_K654034
direction: direction/reverse
start: 945
end: 1073
orientation: inline

BBa_K390002
type: ComponentDefinition
persistent identity: BBa K390002
display id: BBa_K390002
version: 1
was generated by: iGEM to SBOL conversion
description: 5'UTR and consensus RBS from psbA2 in Synechocystis sp. PCC 6803
created: 2010-07-31T11:00:00Z
modified: 2015-05-08T01:12:19Z
mutable provenance: Based on genomic sequence in Synechocystis sp. PCC 6803. Made from annealing of two oligionucleotides that contained the mutations to the consensus RBS.
owned by: james
owned by: myers
mutable description: This is the 5'UTR and RBS region from gene psbA2 in Synechocystis sp. PCC 6803. It covers the entire region from the end of the promoter (as reported in Imamura S and Asayama M. 2009. Sigma Factors for Cyanobacterial Transcription. Gene Regulation and Systems Biology 3: 65-87) to immediately before the start codon of the psbA2 gene. The RBS region has been mutated from the native RBS "taaggaatta" to the consensus RBS "aaaggaggtt" for Synechocystis sp. PCC 6803. This part can serve as a general RBS for Synechocystis sp. PCC 6803, until a library of RBS strengths in PCC 6803 is assembled. The consensus RBS was determined by aligning the 9 nucleotides on the 3' end sequence of the 16S rRNA to the 20 nucleotides preceding the start codon of every gene in PCC 6803. The most frequently represented nucleotide at each location along the alignment was considered to be the consensus. (Note: the alignment was done in relation to the 16S rRNA sequence, and not the start codon, as the spacing between RBS and the start codon varied considerably from gene to gene).
discontinued: false
dominant: false
experience: experience/None
group u list: _518_
m user id: 0
owner id: 6788
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: none
star: false
creator: Cody Tramp
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: 5' UTR with consensus RBS
sequence annotation: t > a
sequence annotation: consensus RBS
sequence annotation: SDS core consensus
sequence annotation: at > gg
sequence annotation: a > t
sequence: https://synbiohub.org/public/igem/BBa_K390002_sequence/1

5' UTR with consensus RBS
type: SequenceAnnotation
persistent identity: annotation2076211
display id: annotation2076211
version: 1
top level: BBa_K390002
location: https://synbiohub.org/public/igem/BBa_K390002/annotation2076211/range2076211/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_K390002/annotation2076211/range2076211/1
type: Range
persistent identity: range2076211
display id: range2076211
version: 1
top level: BBa_K390002
direction: direction/forward
start: 1
end: 49
orientation: inline

t > a
type: SequenceAnnotation
persistent identity: annotation2076289
display id: annotation2076289
version: 1
top level: BBa_K390002
location: https://synbiohub.org/public/igem/BBa_K390002/annotation2076289/range2076289/1
role: SO:0001059
role: feature/mutation

https://synbiohub.org/public/igem/BBa_K390002/annotation2076289/range2076289/1
type: Range
persistent identity: range2076289
display id: range2076289
version: 1
top level: BBa_K390002
direction: direction/forward
start: 33
end: 33
orientation: inline

consensus RBS
type: SequenceAnnotation
persistent identity: annotation2076212
display id: annotation2076212
version: 1
top level: BBa_K390002
location: https://synbiohub.org/public/igem/BBa_K390002/annotation2076212/range2076212/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_K390002/annotation2076212/range2076212/1
type: Range
persistent identity: range2076212
display id: range2076212
version: 1
top level: BBa_K390002
direction: direction/forward
start: 33
end: 42
orientation: inline

SDS core consensus
type: SequenceAnnotation
persistent identity: annotation2076269
display id: annotation2076269
version: 1
top level: BBa_K390002
location: https://synbiohub.org/public/igem/BBa_K390002/annotation2076269/range2076269/1
role: SO:0000139
role: feature/rbs

https://synbiohub.org/public/igem/BBa_K390002/annotation2076269/range2076269/1
type: Range
persistent identity: range2076269
display id: range2076269
version: 1
top level: BBa_K390002
direction: direction/forward
start: 35
end: 40
orientation: inline

at > gg
type: SequenceAnnotation
persistent identity: annotation2076290
display id: annotation2076290
version: 1
top level: BBa_K390002
location: https://synbiohub.org/public/igem/BBa_K390002/annotation2076290/range2076290/1
role: SO:0001059
role: feature/mutation

https://synbiohub.org/public/igem/BBa_K390002/annotation2076290/range2076290/1
type: Range
persistent identity: range2076290
display id: range2076290
version: 1
top level: BBa_K390002
direction: direction/forward
start: 39
end: 40
orientation: inline

a > t
type: SequenceAnnotation
persistent identity: annotation2076291
display id: annotation2076291
version: 1
top level: BBa_K390002
location: https://synbiohub.org/public/igem/BBa_K390002/annotation2076291/range2076291/1
role: feature/mutation
role: SO:0001059

https://synbiohub.org/public/igem/BBa_K390002/annotation2076291/range2076291/1
type: Range
persistent identity: range2076291
display id: range2076291
version: 1
top level: BBa_K390002
direction: direction/forward
start: 42
end: 42
orientation: inline

BBa_K390017
type: ComponentDefinition
persistent identity: BBa K390017
display id: BBa_K390017
version: 1
was generated by: iGEM to SBOL conversion
description: psbA2 promoter, 5'UTR, and RBS
created: 2010-08-01T11:00:00Z
modified: 2015-05-08T01:12:19Z
mutable provenance: (coming soon)
owned by: james
owned by: myers
mutable description: (coming soon)
discontinued: false
dominant: false
group u list: _518_
m user id: 0
owner id: 6788
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: (coming soon)
star: false
creator: Cody Tramp
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: psbA2 promoter
sequence annotation: -35 box
sequence annotation: -10 box
sequence annotation: +1 Transcription Start Point
sequence annotation: putative rbs
sequence: https://synbiohub.org/public/igem/BBa_K390017_sequence/1

psbA2 promoter
type: SequenceAnnotation
persistent identity: annotation2076603
display id: annotation2076603
version: 1
top level: BBa_K390017
location: https://synbiohub.org/public/igem/BBa_K390017/annotation2076603/range2076603/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_K390017/annotation2076603/range2076603/1
type: Range
persistent identity: range2076603
display id: range2076603
version: 1
top level: BBa_K390017
direction: direction/forward
start: 1
end: 38
orientation: inline

-35 box
type: SequenceAnnotation
persistent identity: annotation2076605
display id: annotation2076605
version: 1
top level: BBa_K390017
location: https://synbiohub.org/public/igem/BBa_K390017/annotation2076605/range2076605/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K390017/annotation2076605/range2076605/1
type: Range
persistent identity: range2076605
display id: range2076605
version: 1
top level: BBa_K390017
direction: direction/forward
start: 3
end: 8
orientation: inline

-10 box
type: SequenceAnnotation
persistent identity: annotation2076606
display id: annotation2076606
version: 1
top level: BBa_K390017
location: https://synbiohub.org/public/igem/BBa_K390017/annotation2076606/range2076606/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_K390017/annotation2076606/range2076606/1
type: Range
persistent identity: range2076606
display id: range2076606
version: 1
top level: BBa_K390017
direction: direction/forward
start: 27
end: 32
orientation: inline

+1 Transcription Start Point
type: SequenceAnnotation
persistent identity: annotation2076607
display id: annotation2076607
version: 1
top level: BBa_K390017
location: https://synbiohub.org/public/igem/BBa_K390017/annotation2076607/range2076607/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_K390017/annotation2076607/range2076607/1
type: Range
persistent identity: range2076607
display id: range2076607
version: 1
top level: BBa_K390017
direction: direction/forward
start: 39
end: 39
orientation: inline

putative rbs
type: SequenceAnnotation
persistent identity: annotation2076604
display id: annotation2076604
version: 1
top level: BBa_K390017
location: https://synbiohub.org/public/igem/BBa_K390017/annotation2076604/range2076604/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_K390017/annotation2076604/range2076604/1
type: Range
persistent identity: range2076604
display id: range2076604
version: 1
top level: BBa_K390017
direction: direction/forward
start: 71
end: 80
orientation: inline

BBa_B0015
type: ComponentDefinition
persistent identity: BBa B0015
display id: BBa_B0015
version: 1
was generated by: iGEM to SBOL conversion
description: double terminator (B0010-B0012)
created: 2003-07-16T11:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Double terminator consisting of BBa_B0010 and BBa_B0012
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Reshma Shetty
type: DnaRegion
role: partType/Terminator
role: SO:0000141
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_B0015_sequence/1

BBa_B0012
type: Component
persistent identity: component1916612
display id: component1916612
version: 1
top level: BBa_B0015
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1916610
display id: component1916610
version: 1
top level: BBa_B0015
definition: BBa_B0010
access: public

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1916610
display id: annotation1916610
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
type: Range
persistent identity: range1916610
display id: range1916610
version: 1
top level: BBa_B0015
direction: direction/forward
start: 1
end: 80
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1916612
display id: annotation1916612
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
type: Range
persistent identity: range1916612
display id: range1916612
version: 1
top level: BBa_B0015
direction: direction/forward
start: 89
end: 129
orientation: inline

BBa_I712019
type: ComponentDefinition
persistent identity: BBa I712019
display id: BBa_I712019
version: 1
was generated by: iGEM to SBOL conversion
description: Firefly luciferase - luciferase from Photinus pyralis
created: 2007-10-20T11:00:00Z
modified: 2015-08-31T04:07:46Z
mutable provenance: This firefly luciferase comes from Promega PGL3 vector.
owned by: james
owned by: myers
mutable description: Firefly luciferase is luciferase from Photinus pyralis. By oxidation of substrate luciferin light is produced. Luciferase thus acts as a reporter protein when connected to other proteins or promoters.
discontinued: false
dominant: true
experience: experience/Works
group u list: _130_
m user id: 0
owner id: 1846
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable notes: Part is cloned into pSBAK3 vector with terminator.
star: true
creator: Anja Korenčič
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence: https://synbiohub.org/public/igem/BBa_I712019_sequence/1

BBa_J52008
type: ComponentDefinition
persistent identity: BBa J52008
display id: BBa_J52008
version: 1
was generated by: iGEM to SBOL conversion
description: luciferase: luciferin 2-monooxygenase from Renilla reniformis (EC 1.13.12.5; SwissProt:: P27652)
created: 2006-10-13T11:00:00Z
modified: 2015-08-31T03:54:04Z
mutable provenance: -
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Rluc is a protein called Renilla`s luciferase and it emits light when adding the right substrate. That is why it can bi used as a reported to track other proteins or to monitor the activity of a promotor.
discontinued: false
dominant: true
experience: experience/Works
group u list: _80_
m user id: 0
owner id: 800
owning group id: 80
sample status: In stock
status: status/Available
bookmark: true
mutable notes: Rluc is cloned in BioBrick vector pSB1AK3.
star: true
creator: Monika Ciglic
type: DnaRegion
role: SO:0000804
role: partType/Reporter
sequence annotation: Rluc
sequence: https://synbiohub.org/public/igem/BBa_J52008_sequence/1

Rluc
type: SequenceAnnotation
persistent identity: annotation1902845
display id: annotation1902845
version: 1
top level: BBa_J52008
location: https://synbiohub.org/public/igem/BBa_J52008/annotation1902845/range1902845/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_J52008/annotation1902845/range1902845/1
type: Range
persistent identity: range1902845
display id: range1902845
version: 1
top level: BBa_J52008
direction: direction/forward
start: 1
end: 936
orientation: inline

Rluc
type: SequenceAnnotation
persistent identity: annotation1902833
display id: annotation1902833
version: 1
top level: BBa_J52008
location: https://synbiohub.org/public/igem/BBa_J52008/annotation1902833/range1902833/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_J52008/annotation1902833/range1902833/1
type: Range
persistent identity: range1902833
display id: range1902833
version: 1
top level: BBa_J52008
direction: direction/forward
start: 1
end: 936
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: SO:0000553
role: feature/polya

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

https://synbiohub.org/public/igem/BBa_K654054_sequence/1
type: Sequence
persistent identity: BBa K654054 sequence
display id: BBa_K654054_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: agtcagttccaatctgaacatcgacaaatacaaaaggaggtttaaccaatactagatggaagacgccaaaaacataaagaaaggcccggcgccattctatccgctggaagatggaaccgctggagagcaactgcataaggctatgaagagatacgccctggttcctggaacaattgcttttacagatgcacatatcgaggtggacatcacttacgctgagtacttcgaaatgtccgttcggttggcagaagctatgaaacgatatgggctgaatacaaatcacagaatcgtcgtatgcagtgaaaactctcttcaattctttatgccggtgttgggcgcgttatttatcggagttgcagttgcgcccgcgaacgacatttataatgaacgtgaattgctcaacagtatgggcatttcgcagcctaccgtggtgttcgtttccaaaaaggggttgcaaaaaattttgaacgtgcaaaaaaagctcccaatcatccaaaaaattattatcatggattctaaaacggattaccagggatttcagtcgatgtacacgttcgtcacatctcatctacctcccggttttaatgaatacgattttgtgccagagtccttcgatagggacaagacaattgcactgatcatgaactcctctggatctactggtctgcctaaaggtgtcgctctgcctcatagaactgcctgcgtgagattctcgcatgccagagatcctatttttggcaatcaaatcattccggatactgcgattttaagtgttgttccattccatcacggttttggaatgtttactacactcggatatttgatatgtggatttcgagtcgtcttaatgtatagatttgaagaagagctgtttctgaggagccttcaggattacaagattcaaagtgcgctgctggtgccaaccctattctccttcttcgccaaaagcactctgattgacaaatacgatttatctaatttacacgaaattgcttctggtggcgctcccctctctaaggaagtcggggaagcggttgccaagaggttccatctgccaggtatcaggcaaggatatgggctcactgagactacatcagctattctgattacacccgagggggatgataaaccgggcgcggtcggtaaagttgttccattttttgaagcgaaggttgtggatctggataccgggaaaacgctgggcgttaatcaaagaggcgaactgtgtgtgagaggtcctatgattatgtccggttatgtaaacaatccggaagcgaccaacgccttgattgacaaggatggatggctacattctggagacatagcttactgggacgaagacgaacacttcttcatcgttgaccgcctgaagtctctgattaagtacaaaggctatcaggtggctcccgctgaattggaatccatcttgctccaacaccccaacatcttcgacgcaggtgtcgcaggtcttcccgacgatgacgccggtgaacttcccgccgccgttgttgttttggagcacggaaagacgatgacggaaaaagagatcgtggattacgtcgccagtcaagtaacaaccgcgaaaaagttgcgcggaggagttgtgtttgtggacgaagtaccgaaaggtcttaccggaaaactcgacgcaagaaaaatcagagagatcctcataaaggccaagaagggcggaaagatcgccgtgtaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagaggctttacaaaactctcattaatcctttagactaagtttagtcagttccaatctgaacatcgacaaatacataaggaattataaccaatactagatggcttccaaggtgtacgaccccgagcaacgcaaacgcatgatcactgggcct…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K654037_sequence/1
type: Sequence
persistent identity: BBa K654037 sequence
display id: BBa_K654037_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gctttacaaaactctcattaatcctttagactaagtttagtcagttccaatctgaacatcgacaaatacataaggaattataaccaatactagatggcttccaaggtgtacgaccccgagcaacgcaaacgcatgatcactgggcctcagtggtgggctcgctgcaagcaaatgaacgtgctggactccttcatcaactactatgattccgagaagcacgccgagaacgccgtgatttttctgcatggtaacgctgcctccagctacctgtggaggcacgtcgtgcctcacatcgagcccgtggctagatgcatcatccctgatctgatcggaatgggtaagtccggcaagagcgggaatggctcatatcgcctcctggatcactacaagtacctcaccgcttggttcgagctgctgaaccttccaaagaaaatcatctttgtgggccacgactggggggcttgtctggcctttcactactcctacgagcaccaagacaagatcaaggccatcgtccatgctgagagtgtcgtggacgtgatcgagtcctgggacgagtggcctgacatcgaggaggatatcgccctgatcaagagcgaagagggcgagaaaatggtgcttgagaataacttcttcgtcgagaccatgctcccaagcaagatcatgcggaaactggagcctgaggagttcgctgcctacctggagccattcaaggagaagggcgaggttagacggcctaccctctcctggcctcgcgagatccctctcgttaagggaggcaagcccgacgtcgtccagattgtccgcaactacaacgcctaccttcgggccagcgacgatctgcctaagatgttcatcgagtccgaccctgggttcttttccaacgctattgtcgagggagctaagaagttccctaacaccgagttcgtgaaggtgaagggcctccacttcagccaggaggacgctccagatgaaatgggtaagtacatcaagagcttcgtggagcgcgtgctgaagaacgagcagtaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K654044_sequence/1
type: Sequence
persistent identity: BBa K654044 sequence
display id: BBa_K654044_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: agtcagttccaatctgaacatcgacaaatacaaaaggaggtttaaccaatactagatggaagacgccaaaaacataaagaaaggcccggcgccattctatccgctggaagatggaaccgctggagagcaactgcataaggctatgaagagatacgccctggttcctggaacaattgcttttacagatgcacatatcgaggtggacatcacttacgctgagtacttcgaaatgtccgttcggttggcagaagctatgaaacgatatgggctgaatacaaatcacagaatcgtcgtatgcagtgaaaactctcttcaattctttatgccggtgttgggcgcgttatttatcggagttgcagttgcgcccgcgaacgacatttataatgaacgtgaattgctcaacagtatgggcatttcgcagcctaccgtggtgttcgtttccaaaaaggggttgcaaaaaattttgaacgtgcaaaaaaagctcccaatcatccaaaaaattattatcatggattctaaaacggattaccagggatttcagtcgatgtacacgttcgtcacatctcatctacctcccggttttaatgaatacgattttgtgccagagtccttcgatagggacaagacaattgcactgatcatgaactcctctggatctactggtctgcctaaaggtgtcgctctgcctcatagaactgcctgcgtgagattctcgcatgccagagatcctatttttggcaatcaaatcattccggatactgcgattttaagtgttgttccattccatcacggttttggaatgtttactacactcggatatttgatatgtggatttcgagtcgtcttaatgtatagatttgaagaagagctgtttctgaggagccttcaggattacaagattcaaagtgcgctgctggtgccaaccctattctccttcttcgccaaaagcactctgattgacaaatacgatttatctaatttacacgaaattgcttctggtggcgctcccctctctaaggaagtcggggaagcggttgccaagaggttccatctgccaggtatcaggcaaggatatgggctcactgagactacatcagctattctgattacacccgagggggatgataaaccgggcgcggtcggtaaagttgttccattttttgaagcgaaggttgtggatctggataccgggaaaacgctgggcgttaatcaaagaggcgaactgtgtgtgagaggtcctatgattatgtccggttatgtaaacaatccggaagcgaccaacgccttgattgacaaggatggatggctacattctggagacatagcttactgggacgaagacgaacacttcttcatcgttgaccgcctgaagtctctgattaagtacaaaggctatcaggtggctcccgctgaattggaatccatcttgctccaacaccccaacatcttcgacgcaggtgtcgcaggtcttcccgacgatgacgccggtgaacttcccgccgccgttgttgttttggagcacggaaagacgatgacggaaaaagagatcgtggattacgtcgccagtcaagtaacaaccgcgaaaaagttgcgcggaggagttgtgtttgtggacgaagtaccgaaaggtcttaccggaaaactcgacgcaagaaaaatcagagagatcctcataaaggccaagaagggcggaaagatcgccgtgtaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K654033_sequence/1
type: Sequence
persistent identity: BBa K654033 sequence
display id: BBa_K654033_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggaagacgccaaaaacataaagaaaggcccggcgccattctatccgctggaagatggaaccgctggagagcaactgcataaggctatgaagagatacgccctggttcctggaacaattgcttttacagatgcacatatcgaggtggacatcacttacgctgagtacttcgaaatgtccgttcggttggcagaagctatgaaacgatatgggctgaatacaaatcacagaatcgtcgtatgcagtgaaaactctcttcaattctttatgccggtgttgggcgcgttatttatcggagttgcagttgcgcccgcgaacgacatttataatgaacgtgaattgctcaacagtatgggcatttcgcagcctaccgtggtgttcgtttccaaaaaggggttgcaaaaaattttgaacgtgcaaaaaaagctcccaatcatccaaaaaattattatcatggattctaaaacggattaccagggatttcagtcgatgtacacgttcgtcacatctcatctacctcccggttttaatgaatacgattttgtgccagagtccttcgatagggacaagacaattgcactgatcatgaactcctctggatctactggtctgcctaaaggtgtcgctctgcctcatagaactgcctgcgtgagattctcgcatgccagagatcctatttttggcaatcaaatcattccggatactgcgattttaagtgttgttccattccatcacggttttggaatgtttactacactcggatatttgatatgtggatttcgagtcgtcttaatgtatagatttgaagaagagctgtttctgaggagccttcaggattacaagattcaaagtgcgctgctggtgccaaccctattctccttcttcgccaaaagcactctgattgacaaatacgatttatctaatttacacgaaattgcttctggtggcgctcccctctctaaggaagtcggggaagcggttgccaagaggttccatctgccaggtatcaggcaaggatatgggctcactgagactacatcagctattctgattacacccgagggggatgataaaccgggcgcggtcggtaaagttgttccattttttgaagcgaaggttgtggatctggataccgggaaaacgctgggcgttaatcaaagaggcgaactgtgtgtgagaggtcctatgattatgtccggttatgtaaacaatccggaagcgaccaacgccttgattgacaaggatggatggctacattctggagacatagcttactgggacgaagacgaacacttcttcatcgttgaccgcctgaagtctctgattaagtacaaaggctatcaggtggctcccgctgaattggaatccatcttgctccaacaccccaacatcttcgacgcaggtgtcgcaggtcttcccgacgatgacgccggtgaacttcccgccgccgttgttgttttggagcacggaaagacgatgacggaaaaagagatcgtggattacgtcgccagtcaagtaacaaccgcgaaaaagttgcgcggaggagttgtgtttgtggacgaagtaccgaaaggtcttaccggaaaactcgacgcaagaaaaatcagagagatcctcataaaggccaagaagggcggaaagatcgccgtgtaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K654034_sequence/1
type: Sequence
persistent identity: BBa K654034 sequence
display id: BBa_K654034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggcttccaaggtgtacgaccccgagcaacgcaaacgcatgatcactgggcctcagtggtgggctcgctgcaagcaaatgaacgtgctggactccttcatcaactactatgattccgagaagcacgccgagaacgccgtgatttttctgcatggtaacgctgcctccagctacctgtggaggcacgtcgtgcctcacatcgagcccgtggctagatgcatcatccctgatctgatcggaatgggtaagtccggcaagagcgggaatggctcatatcgcctcctggatcactacaagtacctcaccgcttggttcgagctgctgaaccttccaaagaaaatcatctttgtgggccacgactggggggcttgtctggcctttcactactcctacgagcaccaagacaagatcaaggccatcgtccatgctgagagtgtcgtggacgtgatcgagtcctgggacgagtggcctgacatcgaggaggatatcgccctgatcaagagcgaagagggcgagaaaatggtgcttgagaataacttcttcgtcgagaccatgctcccaagcaagatcatgcggaaactggagcctgaggagttcgctgcctacctggagccattcaaggagaagggcgaggttagacggcctaccctctcctggcctcgcgagatccctctcgttaagggaggcaagcccgacgtcgtccagattgtccgcaactacaacgcctaccttcgggccagcgacgatctgcctaagatgttcatcgagtccgaccctgggttcttttccaacgctattgtcgagggagctaagaagttccctaacaccgagttcgtgaaggtgaagggcctccacttcagccaggaggacgctccagatgaaatgggtaagtacatcaagagcttcgtggagcgcgtgctgaagaacgagcagtaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K390002_sequence/1
type: Sequence
persistent identity: BBa K390002 sequence
display id: BBa_K390002_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: agtcagttccaatctgaacatcgacaaatacaaaaggaggtttaaccaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K390017_sequence/1
type: Sequence
persistent identity: BBa K390017 sequence
display id: BBa_K390017_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gctttacaaaactctcattaatcctttagactaagtttagtcagttccaatctgaacatcgacaaatacataaggaattataaccaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0015_sequence/1
type: Sequence
persistent identity: BBa B0015 sequence
display id: BBa_B0015_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I712019_sequence/1
type: Sequence
persistent identity: BBa I712019 sequence
display id: BBa_I712019_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggaagacgccaaaaacataaagaaaggcccggcgccattctatccgctggaagatggaaccgctggagagcaactgcataaggctatgaagagatacgccctggttcctggaacaattgcttttacagatgcacatatcgaggtggacatcacttacgctgagtacttcgaaatgtccgttcggttggcagaagctatgaaacgatatgggctgaatacaaatcacagaatcgtcgtatgcagtgaaaactctcttcaattctttatgccggtgttgggcgcgttatttatcggagttgcagttgcgcccgcgaacgacatttataatgaacgtgaattgctcaacagtatgggcatttcgcagcctaccgtggtgttcgtttccaaaaaggggttgcaaaaaattttgaacgtgcaaaaaaagctcccaatcatccaaaaaattattatcatggattctaaaacggattaccagggatttcagtcgatgtacacgttcgtcacatctcatctacctcccggttttaatgaatacgattttgtgccagagtccttcgatagggacaagacaattgcactgatcatgaactcctctggatctactggtctgcctaaaggtgtcgctctgcctcatagaactgcctgcgtgagattctcgcatgccagagatcctatttttggcaatcaaatcattccggatactgcgattttaagtgttgttccattccatcacggttttggaatgtttactacactcggatatttgatatgtggatttcgagtcgtcttaatgtatagatttgaagaagagctgtttctgaggagccttcaggattacaagattcaaagtgcgctgctggtgccaaccctattctccttcttcgccaaaagcactctgattgacaaatacgatttatctaatttacacgaaattgcttctggtggcgctcccctctctaaggaagtcggggaagcggttgccaagaggttccatctgccaggtatcaggcaaggatatgggctcactgagactacatcagctattctgattacacccgagggggatgataaaccgggcgcggtcggtaaagttgttccattttttgaagcgaaggttgtggatctggataccgggaaaacgctgggcgttaatcaaagaggcgaactgtgtgtgagaggtcctatgattatgtccggttatgtaaacaatccggaagcgaccaacgccttgattgacaaggatggatggctacattctggagacatagcttactgggacgaagacgaacacttcttcatcgttgaccgcctgaagtctctgattaagtacaaaggctatcaggtggctcccgctgaattggaatccatcttgctccaacaccccaacatcttcgacgcaggtgtcgcaggtcttcccgacgatgacgccggtgaacttcccgccgccgttgttgttttggagcacggaaagacgatgacggaaaaagagatcgtggattacgtcgccagtcaagtaacaaccgcgaaaaagttgcgcggaggagttgtgtttgtggacgaagtaccgaaaggtcttaccggaaaactcgacgcaagaaaaatcagagagatcctcataaaggccaagaagggcggaaagatcgccgtgtaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J52008_sequence/1
type: Sequence
persistent identity: BBa J52008 sequence
display id: BBa_J52008_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggcttccaaggtgtacgaccccgagcaacgcaaacgcatgatcactgggcctcagtggtgggctcgctgcaagcaaatgaacgtgctggactccttcatcaactactatgattccgagaagcacgccgagaacgccgtgatttttctgcatggtaacgctgcctccagctacctgtggaggcacgtcgtgcctcacatcgagcccgtggctagatgcatcatccctgatctgatcggaatgggtaagtccggcaagagcgggaatggctcatatcgcctcctggatcactacaagtacctcaccgcttggttcgagctgctgaaccttccaaagaaaatcatctttgtgggccacgactggggggcttgtctggcctttcactactcctacgagcaccaagacaagatcaaggccatcgtccatgctgagagtgtcgtggacgtgatcgagtcctgggacgagtggcctgacatcgaggaggatatcgccctgatcaagagcgaagagggcgagaaaatggtgcttgagaataacttcttcgtcgagaccatgctcccaagcaagatcatgcggaaactggagcctgaggagttcgctgcctacctggagccattcaaggagaagggcgaggttagacggcctaccctctcctggcctcgcgagatccctctcgttaagggaggcaagcccgacgtcgtccagattgtccgcaactacaacgcctaccttcgggccagcgacgatctgcctaagatgttcatcgagtccgaccctgggttcttttccaacgctattgtcgagggagctaagaagttccctaacaccgagttcgtgaaggtgaagggcctccacttcagccaggaggacgctccagatgaaatgggtaagtacatcaagagcttcgtggagcgcgtgctgaagaacgagcagtaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z