LOCUS       BBa_K654065             1847 bp    DNA     linear       01-Nov-2024
DEFINITION  Fatty Acyl-CoA Synthetase (Ligase) + Terminator
ACCESSION   BBa_K654065
VERSION     BBa_K654065.1  
FEATURES             Location/Qualifiers
     misc_feature    1..3
                     /label=Start
     misc_feature    1708..1710
                     /label=Stop
     misc_feature    1684..1707
                     /label=8-His Tag
     CDS             1..1683
                     /label=fadD acyl-CoA synthetase
     CDS             1..1710
                     /label=BBa_K654059
     stem_loop       1730..1773
                     /label=stem_loop
     misc_feature    1719..1798
                     /label=BBa_B0010
     terminator      1719..1798
                     /label=BBa_B0010
     stem_loop       1814..1833
                     /label=T7 TE
     misc_feature    1840..1840
                     /label=stop
     polyA_site      1834..1847
                     /label=polya
     misc_feature    1807..1847
                     /label=BBa_B0012
     terminator      1807..1847
                     /label=BBa_B0012
     terminator      1719..1847
                     /label=BBa_B0015
ORIGIN
        1 ttgaagaagg tttggcttaa ccgttatccc gcggacgttc cgacggagat caaccctgac
       61 cgttatcaat ctctggtaga tatgtttgag cagtcggtcg cgcgctacgc cgatcaacct
      121 gcgtttgtga atatggggga ggtaatgacc ttccgcaagc tggaagaacg cagtcgcgcg
      181 tttgccgctt atttgcaaca agggttgggg ctgaagaaag gcgatcgcgt tgcgttgatg
      241 atgcctaatt tattgcaata tccggtggcg ctgtttggca ttttgcgtgc cgggatgatc
      301 gtcgtaaacg ttaacccgtt gtataccccg cgtgagcttg agcatcagct taacgatagc
      361 ggcgcatcgg cgattgttat cgtgtctaac tttgctcaca cactggaaaa agtggttgat
      421 aaaaccgccg ttcagcacgt aattctgacc cgtatgggcg atcagctatc tacggcaaaa
      481 ggcacggtag tcaatttcgt tgttaaatac atcaagcgtt tggtgccgaa ataccatctg
      541 ccagatgcca tttcatttcg tagcgcactg cataacggct accggatgca gtacgtcaaa
      601 cccgaactgg tgccggaaga tttagctttt ctgcaataca ccggcggcac cactggtgtg
      661 gcgaaaggcg cgatgctgac tcaccgcaat atgctggcga acctggaaca ggttaacgcg
      721 acctatggtc cgctgttgca tccgggcaaa gagctggtgg tgacggcgct gccgctgtat
      781 cacatttttg ccctgaccat taactgcctg ctgtttatcg aactgggtgg gcagaacctg
      841 cttatcacta acccgcgcga tattccaggg ttggtaaaag agttagcgaa atatccgttt
      901 accgctatca cgggcgttaa caccttgttc aatgcgttgc tgaacaataa agagttccag
      961 cagctggatt tctccagtct gcatctttcc gcaggcggtg ggatgccagt gcagcaagtg
     1021 gtggcagagc gttgggtgaa actgaccgga cagtatctgc tggaaggcta tggccttacc
     1081 gagtgtgcgc cgctggtcag cgttaaccca tatgatattg attatcatag tggtagcatc
     1141 ggtttgccgg tgccgtcgac ggaagccaaa ctggtggatg atgatgataa tgaagtacca
     1201 ccaggtcaac cgggtgagct ttgtgtcaaa ggaccgcagg tgatgctggg ttactggcag
     1261 cgtcccgatg ctaccgatga aatcatcaaa aatggctggt tacacaccgg cgacatcgcg
     1321 gtaatggatg aagaaggatt cctgcgcatt gtcgatcgta aaaaagacat gattctggtt
     1381 tccggtttta acgtctatcc caacgagatt gaagatgtcg tcatgcagca tcctggcgta
     1441 caggaagtcg cggctgttgg cgtaccttcc ggctccagtg gtgaagcggt gaaaatcttc
     1501 gtagtgaaaa aagatccatc gcttaccgaa gagtcactgg tgactttttg ccgccgtcag
     1561 ctcacgggat acaaagtacc gaagctggtg gagtttcgtg atgagttacc gaaatctaac
     1621 gtcggaaaaa ttttgcgacg agaattacgt gacgaagcgc gcggcaaagt ggacaataaa
     1681 gcccatcatc accatcatca ccatcactga tactagagcc aggcatcaaa taaaacgaaa
     1741 ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcta
     1801 ctagagtcac actggctcac cttcgggtgg gcctttctgc gtttata
//