BBa_B0012
1
BBa_B0012
TE from coliphageT7
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
Released HQ 2013
Transcription terminator for the <i>E.coli</i> RNA polymerase.
false
false
_1_
0
24
7
In stock
false
<P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
false
Reshma Shetty
annotation1690
1
polya
range1690
1
28
41
annotation7020
1
BBa_B0012
range7020
1
1
41
annotation1686
1
T7 TE
range1686
1
8
27
annotation1687
1
stop
range1687
1
34
34
BBa_K654060
1
BBa_K654060
Fatty Acyl-CoA Reductase with 8-His Tag
2011-09-25T11:00:00Z
2015-05-08T01:13:01Z
Marinobacter aquaeolei genomic DNA
Maqu_2507 Fatty Acyl CoA Reductase (From Marinobacter aquaeolei) EC 1.2.1.50
false
false
_831_
0
6788
9
It's complicated
true
mutagenesis removal of PstI site, without altering amino acid sequence
false
Cody Tramp
annotation2141922
1
8-His Tag
range2141922
1
1986
2009
annotation2141920
1
Start
range2141920
1
1
3
annotation2141921
1
Stop
range2141921
1
2010
2012
annotation2141924
1
Fatty Acyl-CoA Reductase
range2141924
1
1
1985
annotation2141923
1
A>C
range2141923
1
1632
1632
BBa_K654066
1
BBa_K654066
Fatty Acyl-CoA Reductase + Terminator
2011-09-25T11:00:00Z
2015-05-08T01:13:01Z
to be added
To be added
false
false
_831_
0
8952
9
It's complicated
false
to be added
false
Joshua Ellis
component2143095
1
BBa_K654060
component2143102
1
BBa_B0015
annotation2143095
1
BBa_K654060
range2143095
1
1
2012
annotation2143102
1
BBa_B0015
range2143102
1
2021
2149
BBa_B0015
1
BBa_B0015
double terminator (B0010-B0012)
2003-07-16T11:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
Double terminator consisting of BBa_B0010 and BBa_B0012
false
true
_1_
0
24
7
In stock
false
true
Reshma Shetty
component1916610
1
BBa_B0010
component1916612
1
BBa_B0012
annotation1916612
1
BBa_B0012
range1916612
1
89
129
annotation1916610
1
BBa_B0010
range1916610
1
1
80
BBa_B0010
1
BBa_B0010
T1 from E. coli rrnB
2003-11-19T12:00:00Z
2015-08-31T04:07:20Z
Transcriptional terminator consisting of a 64 bp stem-loop.
false
false
_1_
0
24
7
In stock
false
true
Randy Rettberg
annotation7018
1
BBa_B0010
range7018
1
1
80
annotation4184
1
stem_loop
range4184
1
12
55
BBa_B0010_sequence
1
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
BBa_K654066_sequence
1
atgaattatttcctgacaggcggcaccggttttatcggtcgttttctggttgagaaactcttggcgcgcggcggcaccgtgtatgttctggttcgcgagcagtcccaggacaagctggagcggctccgggagcgctggggtgcagacgacaagcaagtgaaggctgtgatcggcgacctcaccagcaaaaaccttggtattgacgcgaaaacgctgaaatcactgaaaggaaatatcgaccacgtattccatcttgccgcggtctacgacatgggcgcagacgaagaagcccaggccgccaccaatatcgaaggcaccagggcggctgttcaggccgccgaagccatgggcgccaagcatttccatcatgtgtcatccatcgcggcagcgggtctgttcaagggtatcttccgggaggatatgttcgaagaagccgagaagcttgatcatccttacctgcgcaccaagcacgaatccgaaaaagttgtgcgtgaagaatgcaaggttccgttccgcatctaccgccctggtatggtcattggccattcggaaaccggcgaaatggacaaggttgacgggccctattacttcttcaagatgattcagaagatccgtcatgcgttgccccagtgggtacccaccatcggtattgaaggtggccggctgaacattgtgccggtggatttcgtggtcgatgcactggatcacattgcccatctggaaggcgaagatggcaactgtttccatctggtggactccgatccgtataaggtgggtgagatcctcaatattttctgcgaggccggccatgccccccgcatgggtatgcgcatcgattcccggatgttcggttttattccgccgtttattcgccagagcatcaagaatctgcctccggtcaagcgcattactggtgcgcttctggatgacatgggcattccgccctcggtgatgtccttcattaattacccgacccgttttgatacccgggagctggagcgggttctgaagggcacagacattgaggtgccgcgtctgccgtcctatgccccggttatctgggactactgggagcgcaatctggacccggacctgttcaaggaccgcaccctcaagggcacggttgaaggtaaggtttgcgtggtcaccggcgcgacctcgggtattggcctggcaacggcagagaagctggcagaggccggtgccattctggtcattggtgcgcgcaccaaggaaactctggatgaagtggcggccagtctggaggccaagggtggcaacgtgcatgcgtaccagtgcgacttttcggacatggacgactgcgaccgctttgtgaagacggtgctggataatcacggccacgtggatgtactggtgaataacgcgggtcgctccatccgccgctcgctggcgttgtcttttgaccggttccacgattttgagcggaccatgcagctgaactactttggctccgttcggctgatcatgggctttgcgccagccatgctggagcgtcgccgcgggcacgtggtgaatatttcttccatcggggtacttaccaacgctccgcgtttctcggcctatgtctcctcgaaatccgcactggacgcgttcagccgctgtgccgctgccgaatggtcggatcgcaacgtgaccttcaccaccatcaacatgccgttggtgaaaacgccgatgatcgcgcccaccaagatctacgattccgtgccgacgctgacgccggatgaagccgcccagatggtggcggatgcgattgtgtaccggcccaagcgcattgccacccgtcttggcgtgttcgcgcaggttctgcatgcgctggcaccgaagatgggtgagatcattatgaacactggctaccggatgttcccggattctccagcagccgctggcagcaagtccggcgaaaagccgaaagtctctaccgagcaggtggcctttgcggcgattatgcgggggatatactggagcatcatcaccatcatcaccatcactaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_K654060_sequence
1
atgaattatttcctgacaggcggcaccggttttatcggtcgttttctggttgagaaactcttggcgcgcggcggcaccgtgtatgttctggttcgcgagcagtcccaggacaagctggagcggctccgggagcgctggggtgcagacgacaagcaagtgaaggctgtgatcggcgacctcaccagcaaaaaccttggtattgacgcgaaaacgctgaaatcactgaaaggaaatatcgaccacgtattccatcttgccgcggtctacgacatgggcgcagacgaagaagcccaggccgccaccaatatcgaaggcaccagggcggctgttcaggccgccgaagccatgggcgccaagcatttccatcatgtgtcatccatcgcggcagcgggtctgttcaagggtatcttccgggaggatatgttcgaagaagccgagaagcttgatcatccttacctgcgcaccaagcacgaatccgaaaaagttgtgcgtgaagaatgcaaggttccgttccgcatctaccgccctggtatggtcattggccattcggaaaccggcgaaatggacaaggttgacgggccctattacttcttcaagatgattcagaagatccgtcatgcgttgccccagtgggtacccaccatcggtattgaaggtggccggctgaacattgtgccggtggatttcgtggtcgatgcactggatcacattgcccatctggaaggcgaagatggcaactgtttccatctggtggactccgatccgtataaggtgggtgagatcctcaatattttctgcgaggccggccatgccccccgcatgggtatgcgcatcgattcccggatgttcggttttattccgccgtttattcgccagagcatcaagaatctgcctccggtcaagcgcattactggtgcgcttctggatgacatgggcattccgccctcggtgatgtccttcattaattacccgacccgttttgatacccgggagctggagcgggttctgaagggcacagacattgaggtgccgcgtctgccgtcctatgccccggttatctgggactactgggagcgcaatctggacccggacctgttcaaggaccgcaccctcaagggcacggttgaaggtaaggtttgcgtggtcaccggcgcgacctcgggtattggcctggcaacggcagagaagctggcagaggccggtgccattctggtcattggtgcgcgcaccaaggaaactctggatgaagtggcggccagtctggaggccaagggtggcaacgtgcatgcgtaccagtgcgacttttcggacatggacgactgcgaccgctttgtgaagacggtgctggataatcacggccacgtggatgtactggtgaataacgcgggtcgctccatccgccgctcgctggcgttgtcttttgaccggttccacgattttgagcggaccatgcagctgaactactttggctccgttcggctgatcatgggctttgcgccagccatgctggagcgtcgccgcgggcacgtggtgaatatttcttccatcggggtacttaccaacgctccgcgtttctcggcctatgtctcctcgaaatccgcactggacgcgttcagccgctgtgccgctgccgaatggtcggatcgcaacgtgaccttcaccaccatcaacatgccgttggtgaaaacgccgatgatcgcgcccaccaagatctacgattccgtgccgacgctgacgccggatgaagccgcccagatggtggcggatgcgattgtgtaccggcccaagcgcattgccacccgtcttggcgtgttcgcgcaggttctgcatgcgctggcaccgaagatgggtgagatcattatgaacactggctaccggatgttcccggattctccagcagccgctggcagcaagtccggcgaaaagccgaaagtctctaccgagcaggtggcctttgcggcgattatgcgggggatatactggagcatcatcaccatcatcaccatcactaa
BBa_B0012_sequence
1
tcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_B0015_sequence
1
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z