BBa_B0012
1
BBa_B0012
TE from coliphageT7
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
Released HQ 2013
Transcription terminator for the <i>E.coli</i> RNA polymerase.
false
false
_1_
0
24
7
In stock
false
<P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
false
Reshma Shetty
annotation1687
1
stop
range1687
1
34
34
annotation1690
1
polya
range1690
1
28
41
annotation1686
1
T7 TE
range1686
1
8
27
annotation7020
1
BBa_B0012
range7020
1
1
41
BBa_B0015
1
BBa_B0015
double terminator (B0010-B0012)
2003-07-16T11:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
Double terminator consisting of BBa_B0010 and BBa_B0012
false
true
_1_
0
24
7
In stock
false
true
Reshma Shetty
component1916612
1
BBa_B0012
component1916610
1
BBa_B0010
annotation1916612
1
BBa_B0012
range1916612
1
89
129
annotation1916610
1
BBa_B0010
range1916610
1
1
80
BBa_K654067
1
BBa_K654067
Wax Ester Synthase + Terminator
2011-09-25T11:00:00Z
2015-05-08T01:13:01Z
existing parts
to be added
false
false
_831_
0
8952
9
It's complicated
false
none
false
Joshua Ellis
component2143107
1
BBa_K654061
component2143114
1
BBa_B0015
annotation2143114
1
BBa_B0015
range2143114
1
1406
1534
annotation2143107
1
BBa_K654061
range2143107
1
1
1397
BBa_K654061
1
BBa_K654061
Wax Ester Synthase with 8-His Tag
2011-09-25T11:00:00Z
2015-05-08T01:13:01Z
Marinobacter aquaeolei genomic DNA
Maqu_0168 Wax Ester Synthase (from Marinobacter aquaeolei) EC 2.3.1.75
false
false
_831_
0
6788
9
It's complicated
true
none
false
Cody Tramp
annotation2141927
1
8-His Tag
range2141927
1
1371
1394
annotation2141925
1
Start
range2141925
1
1
3
annotation2141926
1
Stop
range2141926
1
1395
1397
annotation2141928
1
Wax Ester Synthase
range2141928
1
1
1370
BBa_B0010
1
BBa_B0010
T1 from E. coli rrnB
2003-11-19T12:00:00Z
2015-08-31T04:07:20Z
Transcriptional terminator consisting of a 64 bp stem-loop.
false
false
_1_
0
24
7
In stock
false
true
Randy Rettberg
annotation4184
1
stem_loop
range4184
1
12
55
annotation7018
1
BBa_B0010
range7018
1
1
80
BBa_B0010_sequence
1
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
BBa_K654067_sequence
1
atgcaccatggaacgcccctgaatcccactgaccagctctttctctggctggaaaaacgccagcagcccatgcatgtgggcggcctccagctgttttccttccccgaaggcgcgccggacgactatgtcgcgcagctggcagaccagcttcggcagaagacggaggtgaccgccccctttaaccagcgcctgagctatcgcctgggccagccggtatgggtggaggatgagcacctggaccttgagcatcatttccgcttcgaggcgctgcccacacccgggcgtattcgggagctgctgtcgttcgtatcggcggagcattcgcacctgatggaccgggagcgccccatgtgggaggtgcacctgatcgagggcctgaaagaccggcagtttgcgctctacaccaaggttcaccattccctggtggacggtgtctcggccatgcgcatggccacccggatgctgagtgaaaacccggacgaacacggcatgccgccaatctgggatctgccttgcctgtcacgggataggggtgagtcggacggacactccctctggcgcagtgtcacccatttgctggggctttcgggccgccagctcggcaccattcccactgtggcaaaggagctactgaaaaccatcaatcaggcccggaaggatccggcctacgactccattttccatgccccgcgctgcatgctgaaccagaaaatcaccggttcccgtcgtttcgccgcccagtcctggtgcctgaaacggattcgcgccgtgtgcgaggcctatggcaccacggtcaacgatgtcgtaactgccatgtgcgcagcggctctgcgtacctatctgatgaatcaggatgccttgccggagaaaccactggtggcctttgtgccggtgtcactacgccgggacgacagctccgggggcaaccaggtaggcgtcatcctggcgagccttcacaccgatgtgcaggaggccggcgaacgactgttaaaaatccaccacggcatggaagaggccaagcagcgctaccggcatatgagcccggaggaaatcgtcaactacacggccctgaccctggcgccggccgccttccacctgctgaccgggctggcgcccaagtggcagacgttcaatgtggtgatttccaatgtccccgggccatccaggcccctgtactggaacggggcgaaactggaaggcatgtatccggtgtctatcgatatggacagactggccctgaacatgacactgaccagctataacgaccaggtggagttcggcctgattggctgtcgccggaccctgcccagcctgcaacggatgctggactacctggaacagggtctggcagagctggagctcaacgccggcatcatcaccatcatcaccatcactaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_B0012_sequence
1
tcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_K654061_sequence
1
atgcaccatggaacgcccctgaatcccactgaccagctctttctctggctggaaaaacgccagcagcccatgcatgtgggcggcctccagctgttttccttccccgaaggcgcgccggacgactatgtcgcgcagctggcagaccagcttcggcagaagacggaggtgaccgccccctttaaccagcgcctgagctatcgcctgggccagccggtatgggtggaggatgagcacctggaccttgagcatcatttccgcttcgaggcgctgcccacacccgggcgtattcgggagctgctgtcgttcgtatcggcggagcattcgcacctgatggaccgggagcgccccatgtgggaggtgcacctgatcgagggcctgaaagaccggcagtttgcgctctacaccaaggttcaccattccctggtggacggtgtctcggccatgcgcatggccacccggatgctgagtgaaaacccggacgaacacggcatgccgccaatctgggatctgccttgcctgtcacgggataggggtgagtcggacggacactccctctggcgcagtgtcacccatttgctggggctttcgggccgccagctcggcaccattcccactgtggcaaaggagctactgaaaaccatcaatcaggcccggaaggatccggcctacgactccattttccatgccccgcgctgcatgctgaaccagaaaatcaccggttcccgtcgtttcgccgcccagtcctggtgcctgaaacggattcgcgccgtgtgcgaggcctatggcaccacggtcaacgatgtcgtaactgccatgtgcgcagcggctctgcgtacctatctgatgaatcaggatgccttgccggagaaaccactggtggcctttgtgccggtgtcactacgccgggacgacagctccgggggcaaccaggtaggcgtcatcctggcgagccttcacaccgatgtgcaggaggccggcgaacgactgttaaaaatccaccacggcatggaagaggccaagcagcgctaccggcatatgagcccggaggaaatcgtcaactacacggccctgaccctggcgccggccgccttccacctgctgaccgggctggcgcccaagtggcagacgttcaatgtggtgatttccaatgtccccgggccatccaggcccctgtactggaacggggcgaaactggaaggcatgtatccggtgtctatcgatatggacagactggccctgaacatgacactgaccagctataacgaccaggtggagttcggcctgattggctgtcgccggaccctgcccagcctgcaacggatgctggactacctggaacagggtctggcagagctggagctcaacgccggcatcatcaccatcatcaccatcactaa
BBa_B0015_sequence
1
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z