LOCUS       BBa_K654070              881 bp    DNA     linear       26-Dec-2024
DEFINITION  psbA2 controlled Thioesterase
ACCESSION   BBa_K654070
VERSION     BBa_K654070.1  
FEATURES             Location/Qualifiers
     promoter        1..38
                     /label=psbA2 promoter
     RBS             71..80
                     /label=putative rbs
     misc_feature    3..8
                     /label=-35 box
     misc_feature    27..32
                     /label=-10 box
     misc_feature    39..39
                     /label=+1 Transcription Start Point
     promoter        1..87
                     /label=BBa_K390017
     misc_feature    94..96
                     /label=Start
     misc_feature    742..744
                     /label=Stop
     misc_feature    718..741
                     /label=8-His Tag
     CDS             94..717
                     /label=Thioesterase (TesA)
     CDS             94..744
                     /label=BBa_K654058
     stem_loop       764..807
                     /label=stem_loop
     misc_feature    753..832
                     /label=BBa_B0010
     terminator      753..832
                     /label=BBa_B0010
     stem_loop       848..867
                     /label=T7 TE
     misc_feature    874..874
                     /label=stop
     polyA_site      868..881
                     /label=polya
     misc_feature    841..881
                     /label=BBa_B0012
     terminator      841..881
                     /label=BBa_B0012
     terminator      753..881
                     /label=BBa_B0015
     misc_feature    94..881
                     /label=BBa_K654064
ORIGIN
        1 gctttacaaa actctcatta atcctttaga ctaagtttag tcagttccaa tctgaacatc
       61 gacaaataca taaggaatta taaccaatac tagatgatga acttcaacaa tgttttccgc
      121 tggcatttgc ccttcctgtt cctggtcctg ttaaccttcc gtgccgccgc agcggacacg
      181 ttattgattc tgggtgatag cctgagcgcc gggtatcgaa tgtctgccag cgcggcctgg
      241 cctgccttgt tgaatgataa gtggcagagt aaaacgtcgg tagttaatgc cagcatcagc
      301 ggcgacacct cgcaacaagg actggcgcgc cttccggctc tgctgaaaca gcatcagccg
      361 cgttgggtgc tggttgaact gggcggcaat gacggtttgc gtggttttca gccacagcaa
      421 accgagcaaa cgctgcgcca gattttgcag gatgtcaaag ccgccaacgc tgaaccattg
      481 ttaatgcaaa tacgtctgcc tgcaaactat ggtcgccgtt ataatgaagc ctttagcgcc
      541 atttacccca aactcgccaa agagtttgat gttccgctgc tgcccttttt tatggaagag
      601 gtctacctca agccacaatg gatgcaggat gacggtattc atcccaaccg cgacgcccag
      661 ccgtttattg ccgactggat ggcgaagcag ttgcagcctt tagtaaatca tgactcacat
      721 catcaccatc atcaccatca ctaatactag agccaggcat caaataaaac gaaaggctca
      781 gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tctactagag
      841 tcacactggc tcaccttcgg gtgggccttt ctgcgtttat a
//