LOCUS       BBa_K654071             1942 bp    DNA     linear       26-Dec-2024
DEFINITION  psbA2 controlled Fatty Acyl-CoA Synthetase (Ligase)
ACCESSION   BBa_K654071
VERSION     BBa_K654071.1  
FEATURES             Location/Qualifiers
     promoter        1..38
                     /label=psbA2 promoter
     RBS             71..80
                     /label=putative rbs
     misc_feature    3..8
                     /label=-35 box
     misc_feature    27..32
                     /label=-10 box
     misc_feature    39..39
                     /label=+1 Transcription Start Point
     promoter        1..87
                     /label=BBa_K390017
     misc_feature    96..98
                     /label=Start
     misc_feature    1803..1805
                     /label=Stop
     misc_feature    1779..1802
                     /label=8-His Tag
     CDS             96..1778
                     /label=fadD acyl-CoA synthetase
     CDS             96..1805
                     /label=BBa_K654059
     stem_loop       1825..1868
                     /label=stem_loop
     misc_feature    1814..1893
                     /label=BBa_B0010
     terminator      1814..1893
                     /label=BBa_B0010
     stem_loop       1909..1928
                     /label=T7 TE
     misc_feature    1935..1935
                     /label=stop
     polyA_site      1929..1942
                     /label=polya
     misc_feature    1902..1942
                     /label=BBa_B0012
     terminator      1902..1942
                     /label=BBa_B0012
     terminator      1814..1942
                     /label=BBa_B0015
     misc_feature    96..1942
                     /label=BBa_K654065
ORIGIN
        1 gctttacaaa actctcatta atcctttaga ctaagtttag tcagttccaa tctgaacatc
       61 gacaaataca taaggaatta taaccaatac tagagttgaa gaaggtttgg cttaaccgtt
      121 atcccgcgga cgttccgacg gagatcaacc ctgaccgtta tcaatctctg gtagatatgt
      181 ttgagcagtc ggtcgcgcgc tacgccgatc aacctgcgtt tgtgaatatg ggggaggtaa
      241 tgaccttccg caagctggaa gaacgcagtc gcgcgtttgc cgcttatttg caacaagggt
      301 tggggctgaa gaaaggcgat cgcgttgcgt tgatgatgcc taatttattg caatatccgg
      361 tggcgctgtt tggcattttg cgtgccggga tgatcgtcgt aaacgttaac ccgttgtata
      421 ccccgcgtga gcttgagcat cagcttaacg atagcggcgc atcggcgatt gttatcgtgt
      481 ctaactttgc tcacacactg gaaaaagtgg ttgataaaac cgccgttcag cacgtaattc
      541 tgacccgtat gggcgatcag ctatctacgg caaaaggcac ggtagtcaat ttcgttgtta
      601 aatacatcaa gcgtttggtg ccgaaatacc atctgccaga tgccatttca tttcgtagcg
      661 cactgcataa cggctaccgg atgcagtacg tcaaacccga actggtgccg gaagatttag
      721 cttttctgca atacaccggc ggcaccactg gtgtggcgaa aggcgcgatg ctgactcacc
      781 gcaatatgct ggcgaacctg gaacaggtta acgcgaccta tggtccgctg ttgcatccgg
      841 gcaaagagct ggtggtgacg gcgctgccgc tgtatcacat ttttgccctg accattaact
      901 gcctgctgtt tatcgaactg ggtgggcaga acctgcttat cactaacccg cgcgatattc
      961 cagggttggt aaaagagtta gcgaaatatc cgtttaccgc tatcacgggc gttaacacct
     1021 tgttcaatgc gttgctgaac aataaagagt tccagcagct ggatttctcc agtctgcatc
     1081 tttccgcagg cggtgggatg ccagtgcagc aagtggtggc agagcgttgg gtgaaactga
     1141 ccggacagta tctgctggaa ggctatggcc ttaccgagtg tgcgccgctg gtcagcgtta
     1201 acccatatga tattgattat catagtggta gcatcggttt gccggtgccg tcgacggaag
     1261 ccaaactggt ggatgatgat gataatgaag taccaccagg tcaaccgggt gagctttgtg
     1321 tcaaaggacc gcaggtgatg ctgggttact ggcagcgtcc cgatgctacc gatgaaatca
     1381 tcaaaaatgg ctggttacac accggcgaca tcgcggtaat ggatgaagaa ggattcctgc
     1441 gcattgtcga tcgtaaaaaa gacatgattc tggtttccgg ttttaacgtc tatcccaacg
     1501 agattgaaga tgtcgtcatg cagcatcctg gcgtacagga agtcgcggct gttggcgtac
     1561 cttccggctc cagtggtgaa gcggtgaaaa tcttcgtagt gaaaaaagat ccatcgctta
     1621 ccgaagagtc actggtgact ttttgccgcc gtcagctcac gggatacaaa gtaccgaagc
     1681 tggtggagtt tcgtgatgag ttaccgaaat ctaacgtcgg aaaaattttg cgacgagaat
     1741 tacgtgacga agcgcgcggc aaagtggaca ataaagccca tcatcaccat catcaccatc
     1801 actgatacta gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt
     1861 cgttttatct gttgtttgtc ggtgaacgct ctctactaga gtcacactgg ctcaccttcg
     1921 ggtgggcctt tctgcgttta ta
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