BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation4184 1 stem_loop range4184 1 12 55 annotation7018 1 BBa_B0010 range7018 1 1 80 BBa_K654067 1 BBa_K654067 Wax Ester Synthase + Terminator 2011-09-25T11:00:00Z 2015-05-08T01:13:01Z existing parts to be added false false _831_ 0 8952 9 It's complicated false none false Joshua Ellis component2143107 1 BBa_K654061 component2143114 1 BBa_B0015 annotation2143107 1 BBa_K654061 range2143107 1 1 1397 annotation2143114 1 BBa_B0015 range2143114 1 1406 1534 BBa_K654061 1 BBa_K654061 Wax Ester Synthase with 8-His Tag 2011-09-25T11:00:00Z 2015-05-08T01:13:01Z Marinobacter aquaeolei genomic DNA Maqu_0168 Wax Ester Synthase (from Marinobacter aquaeolei) EC 2.3.1.75 false false _831_ 0 6788 9 It's complicated true none false Cody Tramp annotation2141928 1 Wax Ester Synthase range2141928 1 1 1370 annotation2141927 1 8-His Tag range2141927 1 1371 1394 annotation2141926 1 Stop range2141926 1 1395 1397 annotation2141925 1 Start range2141925 1 1 3 BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation1690 1 polya range1690 1 28 41 annotation7020 1 BBa_B0012 range7020 1 1 41 annotation1687 1 stop range1687 1 34 34 annotation1686 1 T7 TE range1686 1 8 27 BBa_K654073 1 BBa_K654073 psbA2 controlled Wax Ester Synthase 2011-09-25T11:00:00Z 2015-05-08T01:13:01Z existing parts to be added false false _831_ 0 8952 9 It's complicated false none false Joshua Ellis component2143195 1 BBa_K654067 component2143182 1 BBa_K390017 annotation2143182 1 BBa_K390017 range2143182 1 1 87 annotation2143195 1 BBa_K654067 range2143195 1 94 1627 BBa_B0015 1 BBa_B0015 double terminator (B0010-B0012) 2003-07-16T11:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 Double terminator consisting of BBa_B0010 and BBa_B0012 false true _1_ 0 24 7 In stock false true Reshma Shetty component1916612 1 BBa_B0012 component1916610 1 BBa_B0010 annotation1916612 1 BBa_B0012 range1916612 1 89 129 annotation1916610 1 BBa_B0010 range1916610 1 1 80 BBa_K390017 1 BBa_K390017 psbA2 promoter, 5'UTR, and RBS 2010-08-01T11:00:00Z 2015-05-08T01:12:19Z (coming soon) (coming soon) false false _518_ 0 6788 9 It's complicated false (coming soon) false Cody Tramp annotation2076603 1 psbA2 promoter range2076603 1 1 38 annotation2076605 1 -35 box range2076605 1 3 8 annotation2076607 1 +1 Transcription Start Point range2076607 1 39 39 annotation2076606 1 -10 box range2076606 1 27 32 annotation2076604 1 putative rbs range2076604 1 71 80 BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_K654073_sequence 1 gctttacaaaactctcattaatcctttagactaagtttagtcagttccaatctgaacatcgacaaatacataaggaattataaccaatactagatgcaccatggaacgcccctgaatcccactgaccagctctttctctggctggaaaaacgccagcagcccatgcatgtgggcggcctccagctgttttccttccccgaaggcgcgccggacgactatgtcgcgcagctggcagaccagcttcggcagaagacggaggtgaccgccccctttaaccagcgcctgagctatcgcctgggccagccggtatgggtggaggatgagcacctggaccttgagcatcatttccgcttcgaggcgctgcccacacccgggcgtattcgggagctgctgtcgttcgtatcggcggagcattcgcacctgatggaccgggagcgccccatgtgggaggtgcacctgatcgagggcctgaaagaccggcagtttgcgctctacaccaaggttcaccattccctggtggacggtgtctcggccatgcgcatggccacccggatgctgagtgaaaacccggacgaacacggcatgccgccaatctgggatctgccttgcctgtcacgggataggggtgagtcggacggacactccctctggcgcagtgtcacccatttgctggggctttcgggccgccagctcggcaccattcccactgtggcaaaggagctactgaaaaccatcaatcaggcccggaaggatccggcctacgactccattttccatgccccgcgctgcatgctgaaccagaaaatcaccggttcccgtcgtttcgccgcccagtcctggtgcctgaaacggattcgcgccgtgtgcgaggcctatggcaccacggtcaacgatgtcgtaactgccatgtgcgcagcggctctgcgtacctatctgatgaatcaggatgccttgccggagaaaccactggtggcctttgtgccggtgtcactacgccgggacgacagctccgggggcaaccaggtaggcgtcatcctggcgagccttcacaccgatgtgcaggaggccggcgaacgactgttaaaaatccaccacggcatggaagaggccaagcagcgctaccggcatatgagcccggaggaaatcgtcaactacacggccctgaccctggcgccggccgccttccacctgctgaccgggctggcgcccaagtggcagacgttcaatgtggtgatttccaatgtccccgggccatccaggcccctgtactggaacggggcgaaactggaaggcatgtatccggtgtctatcgatatggacagactggccctgaacatgacactgaccagctataacgaccaggtggagttcggcctgattggctgtcgccggaccctgcccagcctgcaacggatgctggactacctggaacagggtctggcagagctggagctcaacgccggcatcatcaccatcatcaccatcactaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_K654067_sequence 1 atgcaccatggaacgcccctgaatcccactgaccagctctttctctggctggaaaaacgccagcagcccatgcatgtgggcggcctccagctgttttccttccccgaaggcgcgccggacgactatgtcgcgcagctggcagaccagcttcggcagaagacggaggtgaccgccccctttaaccagcgcctgagctatcgcctgggccagccggtatgggtggaggatgagcacctggaccttgagcatcatttccgcttcgaggcgctgcccacacccgggcgtattcgggagctgctgtcgttcgtatcggcggagcattcgcacctgatggaccgggagcgccccatgtgggaggtgcacctgatcgagggcctgaaagaccggcagtttgcgctctacaccaaggttcaccattccctggtggacggtgtctcggccatgcgcatggccacccggatgctgagtgaaaacccggacgaacacggcatgccgccaatctgggatctgccttgcctgtcacgggataggggtgagtcggacggacactccctctggcgcagtgtcacccatttgctggggctttcgggccgccagctcggcaccattcccactgtggcaaaggagctactgaaaaccatcaatcaggcccggaaggatccggcctacgactccattttccatgccccgcgctgcatgctgaaccagaaaatcaccggttcccgtcgtttcgccgcccagtcctggtgcctgaaacggattcgcgccgtgtgcgaggcctatggcaccacggtcaacgatgtcgtaactgccatgtgcgcagcggctctgcgtacctatctgatgaatcaggatgccttgccggagaaaccactggtggcctttgtgccggtgtcactacgccgggacgacagctccgggggcaaccaggtaggcgtcatcctggcgagccttcacaccgatgtgcaggaggccggcgaacgactgttaaaaatccaccacggcatggaagaggccaagcagcgctaccggcatatgagcccggaggaaatcgtcaactacacggccctgaccctggcgccggccgccttccacctgctgaccgggctggcgcccaagtggcagacgttcaatgtggtgatttccaatgtccccgggccatccaggcccctgtactggaacggggcgaaactggaaggcatgtatccggtgtctatcgatatggacagactggccctgaacatgacactgaccagctataacgaccaggtggagttcggcctgattggctgtcgccggaccctgcccagcctgcaacggatgctggactacctggaacagggtctggcagagctggagctcaacgccggcatcatcaccatcatcaccatcactaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_K390017_sequence 1 gctttacaaaactctcattaatcctttagactaagtttagtcagttccaatctgaacatcgacaaatacataaggaattataaccaa BBa_K654061_sequence 1 atgcaccatggaacgcccctgaatcccactgaccagctctttctctggctggaaaaacgccagcagcccatgcatgtgggcggcctccagctgttttccttccccgaaggcgcgccggacgactatgtcgcgcagctggcagaccagcttcggcagaagacggaggtgaccgccccctttaaccagcgcctgagctatcgcctgggccagccggtatgggtggaggatgagcacctggaccttgagcatcatttccgcttcgaggcgctgcccacacccgggcgtattcgggagctgctgtcgttcgtatcggcggagcattcgcacctgatggaccgggagcgccccatgtgggaggtgcacctgatcgagggcctgaaagaccggcagtttgcgctctacaccaaggttcaccattccctggtggacggtgtctcggccatgcgcatggccacccggatgctgagtgaaaacccggacgaacacggcatgccgccaatctgggatctgccttgcctgtcacgggataggggtgagtcggacggacactccctctggcgcagtgtcacccatttgctggggctttcgggccgccagctcggcaccattcccactgtggcaaaggagctactgaaaaccatcaatcaggcccggaaggatccggcctacgactccattttccatgccccgcgctgcatgctgaaccagaaaatcaccggttcccgtcgtttcgccgcccagtcctggtgcctgaaacggattcgcgccgtgtgcgaggcctatggcaccacggtcaacgatgtcgtaactgccatgtgcgcagcggctctgcgtacctatctgatgaatcaggatgccttgccggagaaaccactggtggcctttgtgccggtgtcactacgccgggacgacagctccgggggcaaccaggtaggcgtcatcctggcgagccttcacaccgatgtgcaggaggccggcgaacgactgttaaaaatccaccacggcatggaagaggccaagcagcgctaccggcatatgagcccggaggaaatcgtcaactacacggccctgaccctggcgccggccgccttccacctgctgaccgggctggcgcccaagtggcagacgttcaatgtggtgatttccaatgtccccgggccatccaggcccctgtactggaacggggcgaaactggaaggcatgtatccggtgtctatcgatatggacagactggccctgaacatgacactgaccagctataacgaccaggtggagttcggcctgattggctgtcgccggaccctgcccagcctgcaacggatgctggactacctggaacagggtctggcagagctggagctcaacgccggcatcatcaccatcatcaccatcactaa BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata BBa_B0015_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z