LOCUS       BBa_K654076              855 bp    DNA     linear       23-Dec-2024
DEFINITION  sigA controlled Thioesterase
ACCESSION   BBa_K654076
VERSION     BBa_K654076.1  
FEATURES             Location/Qualifiers
     promoter        4..45
                     /label=sigA promoter
     RBS             46..55
                     /label=putative rbs
     misc_feature    46..46
                     /label=+1 Transcription Start Point
     misc_feature    9..14
                     /label=-35 box
     misc_feature    30..35
                     /label=-10 box
     promoter        1..61
                     /label=BBa_K390018
     misc_feature    68..70
                     /label=Start
     misc_feature    716..718
                     /label=Stop
     misc_feature    692..715
                     /label=8-His Tag
     CDS             68..691
                     /label=Thioesterase (TesA)
     CDS             68..718
                     /label=BBa_K654058
     stem_loop       738..781
                     /label=stem_loop
     misc_feature    727..806
                     /label=BBa_B0010
     terminator      727..806
                     /label=BBa_B0010
     stem_loop       822..841
                     /label=T7 TE
     misc_feature    848..848
                     /label=stop
     polyA_site      842..855
                     /label=polya
     misc_feature    815..855
                     /label=BBa_B0012
     terminator      815..855
                     /label=BBa_B0012
     terminator      727..855
                     /label=BBa_B0015
     misc_feature    68..855
                     /label=BBa_K654064
ORIGIN
        1 cctcaacttt gactgaccag ctaattttgt acacgactta ggagttaacg aggaaaacgg
       61 ctactagatg atgaacttca acaatgtttt ccgctggcat ttgcccttcc tgttcctggt
      121 cctgttaacc ttccgtgccg ccgcagcgga cacgttattg attctgggtg atagcctgag
      181 cgccgggtat cgaatgtctg ccagcgcggc ctggcctgcc ttgttgaatg ataagtggca
      241 gagtaaaacg tcggtagtta atgccagcat cagcggcgac acctcgcaac aaggactggc
      301 gcgccttccg gctctgctga aacagcatca gccgcgttgg gtgctggttg aactgggcgg
      361 caatgacggt ttgcgtggtt ttcagccaca gcaaaccgag caaacgctgc gccagatttt
      421 gcaggatgtc aaagccgcca acgctgaacc attgttaatg caaatacgtc tgcctgcaaa
      481 ctatggtcgc cgttataatg aagcctttag cgccatttac cccaaactcg ccaaagagtt
      541 tgatgttccg ctgctgccct tttttatgga agaggtctac ctcaagccac aatggatgca
      601 ggatgacggt attcatccca accgcgacgc ccagccgttt attgccgact ggatggcgaa
      661 gcagttgcag cctttagtaa atcatgactc acatcatcac catcatcacc atcactaata
      721 ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta
      781 tctgttgttt gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc
      841 ctttctgcgt ttata
//