LOCUS BBa_K654077 1916 bp DNA linear 22-Nov-2024 DEFINITION sigA controlled Fatty Acyl-CoA Synthetase (Ligase) ACCESSION BBa_K654077 VERSION BBa_K654077.1 FEATURES Location/Qualifiers promoter 4..45 /label=sigA promoter RBS 46..55 /label=putative rbs misc_feature 46..46 /label=+1 Transcription Start Point misc_feature 9..14 /label=-35 box misc_feature 30..35 /label=-10 box promoter 1..61 /label=BBa_K390018 misc_feature 70..72 /label=Start misc_feature 1777..1779 /label=Stop misc_feature 1753..1776 /label=8-His Tag CDS 70..1752 /label=fadD acyl-CoA synthetase CDS 70..1779 /label=BBa_K654059 stem_loop 1799..1842 /label=stem_loop misc_feature 1788..1867 /label=BBa_B0010 terminator 1788..1867 /label=BBa_B0010 stem_loop 1883..1902 /label=T7 TE misc_feature 1909..1909 /label=stop polyA_site 1903..1916 /label=polya misc_feature 1876..1916 /label=BBa_B0012 terminator 1876..1916 /label=BBa_B0012 terminator 1788..1916 /label=BBa_B0015 misc_feature 70..1916 /label=BBa_K654065 ORIGIN 1 cctcaacttt gactgaccag ctaattttgt acacgactta ggagttaacg aggaaaacgg 61 ctactagagt tgaagaaggt ttggcttaac cgttatcccg cggacgttcc gacggagatc 121 aaccctgacc gttatcaatc tctggtagat atgtttgagc agtcggtcgc gcgctacgcc 181 gatcaacctg cgtttgtgaa tatgggggag gtaatgacct tccgcaagct ggaagaacgc 241 agtcgcgcgt ttgccgctta tttgcaacaa gggttggggc tgaagaaagg cgatcgcgtt 301 gcgttgatga tgcctaattt attgcaatat ccggtggcgc tgtttggcat tttgcgtgcc 361 gggatgatcg tcgtaaacgt taacccgttg tataccccgc gtgagcttga gcatcagctt 421 aacgatagcg gcgcatcggc gattgttatc gtgtctaact ttgctcacac actggaaaaa 481 gtggttgata aaaccgccgt tcagcacgta attctgaccc gtatgggcga tcagctatct 541 acggcaaaag gcacggtagt caatttcgtt gttaaataca tcaagcgttt ggtgccgaaa 601 taccatctgc cagatgccat ttcatttcgt agcgcactgc ataacggcta ccggatgcag 661 tacgtcaaac ccgaactggt gccggaagat ttagcttttc tgcaatacac cggcggcacc 721 actggtgtgg cgaaaggcgc gatgctgact caccgcaata tgctggcgaa cctggaacag 781 gttaacgcga cctatggtcc gctgttgcat ccgggcaaag agctggtggt gacggcgctg 841 ccgctgtatc acatttttgc cctgaccatt aactgcctgc tgtttatcga actgggtggg 901 cagaacctgc ttatcactaa cccgcgcgat attccagggt tggtaaaaga gttagcgaaa 961 tatccgttta ccgctatcac gggcgttaac accttgttca atgcgttgct gaacaataaa 1021 gagttccagc agctggattt ctccagtctg catctttccg caggcggtgg gatgccagtg 1081 cagcaagtgg tggcagagcg ttgggtgaaa ctgaccggac agtatctgct ggaaggctat 1141 ggccttaccg agtgtgcgcc gctggtcagc gttaacccat atgatattga ttatcatagt 1201 ggtagcatcg gtttgccggt gccgtcgacg gaagccaaac tggtggatga tgatgataat 1261 gaagtaccac caggtcaacc gggtgagctt tgtgtcaaag gaccgcaggt gatgctgggt 1321 tactggcagc gtcccgatgc taccgatgaa atcatcaaaa atggctggtt acacaccggc 1381 gacatcgcgg taatggatga agaaggattc ctgcgcattg tcgatcgtaa aaaagacatg 1441 attctggttt ccggttttaa cgtctatccc aacgagattg aagatgtcgt catgcagcat 1501 cctggcgtac aggaagtcgc ggctgttggc gtaccttccg gctccagtgg tgaagcggtg 1561 aaaatcttcg tagtgaaaaa agatccatcg cttaccgaag agtcactggt gactttttgc 1621 cgccgtcagc tcacgggata caaagtaccg aagctggtgg agtttcgtga tgagttaccg 1681 aaatctaacg tcggaaaaat tttgcgacga gaattacgtg acgaagcgcg cggcaaagtg 1741 gacaataaag cccatcatca ccatcatcac catcactgat actagagcca ggcatcaaat 1801 aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa 1861 cgctctctac tagagtcaca ctggctcacc ttcgggtggg cctttctgcg tttata //