LOCUS       BBa_K654077             1916 bp    DNA     linear       01-Nov-2024
DEFINITION  sigA controlled Fatty Acyl-CoA Synthetase (Ligase)
ACCESSION   BBa_K654077
VERSION     BBa_K654077.1  
FEATURES             Location/Qualifiers
     promoter        4..45
                     /label=sigA promoter
     RBS             46..55
                     /label=putative rbs
     misc_feature    46..46
                     /label=+1 Transcription Start Point
     misc_feature    9..14
                     /label=-35 box
     misc_feature    30..35
                     /label=-10 box
     promoter        1..61
                     /label=BBa_K390018
     misc_feature    70..72
                     /label=Start
     misc_feature    1777..1779
                     /label=Stop
     misc_feature    1753..1776
                     /label=8-His Tag
     CDS             70..1752
                     /label=fadD acyl-CoA synthetase
     CDS             70..1779
                     /label=BBa_K654059
     stem_loop       1799..1842
                     /label=stem_loop
     misc_feature    1788..1867
                     /label=BBa_B0010
     terminator      1788..1867
                     /label=BBa_B0010
     stem_loop       1883..1902
                     /label=T7 TE
     misc_feature    1909..1909
                     /label=stop
     polyA_site      1903..1916
                     /label=polya
     misc_feature    1876..1916
                     /label=BBa_B0012
     terminator      1876..1916
                     /label=BBa_B0012
     terminator      1788..1916
                     /label=BBa_B0015
     misc_feature    70..1916
                     /label=BBa_K654065
ORIGIN
        1 cctcaacttt gactgaccag ctaattttgt acacgactta ggagttaacg aggaaaacgg
       61 ctactagagt tgaagaaggt ttggcttaac cgttatcccg cggacgttcc gacggagatc
      121 aaccctgacc gttatcaatc tctggtagat atgtttgagc agtcggtcgc gcgctacgcc
      181 gatcaacctg cgtttgtgaa tatgggggag gtaatgacct tccgcaagct ggaagaacgc
      241 agtcgcgcgt ttgccgctta tttgcaacaa gggttggggc tgaagaaagg cgatcgcgtt
      301 gcgttgatga tgcctaattt attgcaatat ccggtggcgc tgtttggcat tttgcgtgcc
      361 gggatgatcg tcgtaaacgt taacccgttg tataccccgc gtgagcttga gcatcagctt
      421 aacgatagcg gcgcatcggc gattgttatc gtgtctaact ttgctcacac actggaaaaa
      481 gtggttgata aaaccgccgt tcagcacgta attctgaccc gtatgggcga tcagctatct
      541 acggcaaaag gcacggtagt caatttcgtt gttaaataca tcaagcgttt ggtgccgaaa
      601 taccatctgc cagatgccat ttcatttcgt agcgcactgc ataacggcta ccggatgcag
      661 tacgtcaaac ccgaactggt gccggaagat ttagcttttc tgcaatacac cggcggcacc
      721 actggtgtgg cgaaaggcgc gatgctgact caccgcaata tgctggcgaa cctggaacag
      781 gttaacgcga cctatggtcc gctgttgcat ccgggcaaag agctggtggt gacggcgctg
      841 ccgctgtatc acatttttgc cctgaccatt aactgcctgc tgtttatcga actgggtggg
      901 cagaacctgc ttatcactaa cccgcgcgat attccagggt tggtaaaaga gttagcgaaa
      961 tatccgttta ccgctatcac gggcgttaac accttgttca atgcgttgct gaacaataaa
     1021 gagttccagc agctggattt ctccagtctg catctttccg caggcggtgg gatgccagtg
     1081 cagcaagtgg tggcagagcg ttgggtgaaa ctgaccggac agtatctgct ggaaggctat
     1141 ggccttaccg agtgtgcgcc gctggtcagc gttaacccat atgatattga ttatcatagt
     1201 ggtagcatcg gtttgccggt gccgtcgacg gaagccaaac tggtggatga tgatgataat
     1261 gaagtaccac caggtcaacc gggtgagctt tgtgtcaaag gaccgcaggt gatgctgggt
     1321 tactggcagc gtcccgatgc taccgatgaa atcatcaaaa atggctggtt acacaccggc
     1381 gacatcgcgg taatggatga agaaggattc ctgcgcattg tcgatcgtaa aaaagacatg
     1441 attctggttt ccggttttaa cgtctatccc aacgagattg aagatgtcgt catgcagcat
     1501 cctggcgtac aggaagtcgc ggctgttggc gtaccttccg gctccagtgg tgaagcggtg
     1561 aaaatcttcg tagtgaaaaa agatccatcg cttaccgaag agtcactggt gactttttgc
     1621 cgccgtcagc tcacgggata caaagtaccg aagctggtgg agtttcgtga tgagttaccg
     1681 aaatctaacg tcggaaaaat tttgcgacga gaattacgtg acgaagcgcg cggcaaagtg
     1741 gacaataaag cccatcatca ccatcatcac catcactgat actagagcca ggcatcaaat
     1801 aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa
     1861 cgctctctac tagagtcaca ctggctcacc ttcgggtggg cctttctgcg tttata
//