LOCUS       BBa_K654078             2216 bp    DNA     linear       29-Nov-2024
DEFINITION  sigA controlled Fatty Acyl-CoA Reductase
ACCESSION   BBa_K654078
VERSION     BBa_K654078.1  
FEATURES             Location/Qualifiers
     promoter        4..45
                     /label=sigA promoter
     RBS             46..55
                     /label=putative rbs
     misc_feature    46..46
                     /label=+1 Transcription Start Point
     misc_feature    9..14
                     /label=-35 box
     misc_feature    30..35
                     /label=-10 box
     promoter        1..61
                     /label=BBa_K390018
     misc_feature    68..70
                     /label=Start
     misc_feature    2077..2079
                     /label=Stop
     misc_feature    2053..2076
                     /label=8-His Tag
     misc_feature    1699..1699
                     /label=A>C
     CDS             68..2052
                     /label=Fatty Acyl-CoA Reductase
     CDS             68..2079
                     /label=BBa_K654060
     stem_loop       2099..2142
                     /label=stem_loop
     misc_feature    2088..2167
                     /label=BBa_B0010
     terminator      2088..2167
                     /label=BBa_B0010
     stem_loop       2183..2202
                     /label=T7 TE
     misc_feature    2209..2209
                     /label=stop
     polyA_site      2203..2216
                     /label=polya
     misc_feature    2176..2216
                     /label=BBa_B0012
     terminator      2176..2216
                     /label=BBa_B0012
     terminator      2088..2216
                     /label=BBa_B0015
     misc_feature    68..2216
                     /label=BBa_K654066
ORIGIN
        1 cctcaacttt gactgaccag ctaattttgt acacgactta ggagttaacg aggaaaacgg
       61 ctactagatg aattatttcc tgacaggcgg caccggtttt atcggtcgtt ttctggttga
      121 gaaactcttg gcgcgcggcg gcaccgtgta tgttctggtt cgcgagcagt cccaggacaa
      181 gctggagcgg ctccgggagc gctggggtgc agacgacaag caagtgaagg ctgtgatcgg
      241 cgacctcacc agcaaaaacc ttggtattga cgcgaaaacg ctgaaatcac tgaaaggaaa
      301 tatcgaccac gtattccatc ttgccgcggt ctacgacatg ggcgcagacg aagaagccca
      361 ggccgccacc aatatcgaag gcaccagggc ggctgttcag gccgccgaag ccatgggcgc
      421 caagcatttc catcatgtgt catccatcgc ggcagcgggt ctgttcaagg gtatcttccg
      481 ggaggatatg ttcgaagaag ccgagaagct tgatcatcct tacctgcgca ccaagcacga
      541 atccgaaaaa gttgtgcgtg aagaatgcaa ggttccgttc cgcatctacc gccctggtat
      601 ggtcattggc cattcggaaa ccggcgaaat ggacaaggtt gacgggccct attacttctt
      661 caagatgatt cagaagatcc gtcatgcgtt gccccagtgg gtacccacca tcggtattga
      721 aggtggccgg ctgaacattg tgccggtgga tttcgtggtc gatgcactgg atcacattgc
      781 ccatctggaa ggcgaagatg gcaactgttt ccatctggtg gactccgatc cgtataaggt
      841 gggtgagatc ctcaatattt tctgcgaggc cggccatgcc ccccgcatgg gtatgcgcat
      901 cgattcccgg atgttcggtt ttattccgcc gtttattcgc cagagcatca agaatctgcc
      961 tccggtcaag cgcattactg gtgcgcttct ggatgacatg ggcattccgc cctcggtgat
     1021 gtccttcatt aattacccga cccgttttga tacccgggag ctggagcggg ttctgaaggg
     1081 cacagacatt gaggtgccgc gtctgccgtc ctatgccccg gttatctggg actactggga
     1141 gcgcaatctg gacccggacc tgttcaagga ccgcaccctc aagggcacgg ttgaaggtaa
     1201 ggtttgcgtg gtcaccggcg cgacctcggg tattggcctg gcaacggcag agaagctggc
     1261 agaggccggt gccattctgg tcattggtgc gcgcaccaag gaaactctgg atgaagtggc
     1321 ggccagtctg gaggccaagg gtggcaacgt gcatgcgtac cagtgcgact tttcggacat
     1381 ggacgactgc gaccgctttg tgaagacggt gctggataat cacggccacg tggatgtact
     1441 ggtgaataac gcgggtcgct ccatccgccg ctcgctggcg ttgtcttttg accggttcca
     1501 cgattttgag cggaccatgc agctgaacta ctttggctcc gttcggctga tcatgggctt
     1561 tgcgccagcc atgctggagc gtcgccgcgg gcacgtggtg aatatttctt ccatcggggt
     1621 acttaccaac gctccgcgtt tctcggccta tgtctcctcg aaatccgcac tggacgcgtt
     1681 cagccgctgt gccgctgccg aatggtcgga tcgcaacgtg accttcacca ccatcaacat
     1741 gccgttggtg aaaacgccga tgatcgcgcc caccaagatc tacgattccg tgccgacgct
     1801 gacgccggat gaagccgccc agatggtggc ggatgcgatt gtgtaccggc ccaagcgcat
     1861 tgccacccgt cttggcgtgt tcgcgcaggt tctgcatgcg ctggcaccga agatgggtga
     1921 gatcattatg aacactggct accggatgtt cccggattct ccagcagccg ctggcagcaa
     1981 gtccggcgaa aagccgaaag tctctaccga gcaggtggcc tttgcggcga ttatgcgggg
     2041 gatatactgg agcatcatca ccatcatcac catcactaat actagagcca ggcatcaaat
     2101 aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa
     2161 cgctctctac tagagtcaca ctggctcacc ttcgggtggg cctttctgcg tttata
//