LOCUS BBa_K654082 2831 bp DNA linear 20-Nov-2024 DEFINITION psbA2 controlled Thioesterase + Acyl-CoA Synthetase ACCESSION BBa_K654082 VERSION BBa_K654082.1 FEATURES Location/Qualifiers promoter 1..38 /label=psbA2 promoter RBS 71..80 /label=putative rbs misc_feature 3..8 /label=-35 box misc_feature 27..32 /label=-10 box misc_feature 39..39 /label=+1 Transcription Start Point promoter 1..87 /label=BBa_K390017 misc_feature 94..96 /label=Start misc_feature 742..744 /label=Stop misc_feature 718..741 /label=8-His Tag CDS 94..717 /label=Thioesterase (TesA) CDS 94..744 /label=BBa_K654058 stem_loop 764..807 /label=stem_loop misc_feature 753..832 /label=BBa_B0010 terminator 753..832 /label=BBa_B0010 stem_loop 848..867 /label=T7 TE misc_feature 874..874 /label=stop polyA_site 868..881 /label=polya misc_feature 841..881 /label=BBa_B0012 terminator 841..881 /label=BBa_B0012 terminator 753..881 /label=BBa_B0015 misc_feature 94..881 /label=BBa_K654064 misc_feature 1..881 /label=BBa_K654070 promoter 890..927 /label=psbA2 promoter RBS 960..969 /label=putative rbs misc_feature 892..897 /label=-35 box misc_feature 916..921 /label=-10 box misc_feature 928..928 /label=+1 Transcription Start Point promoter 890..976 /label=BBa_K390017 misc_feature 985..987 /label=Start misc_feature 2692..2694 /label=Stop misc_feature 2668..2691 /label=8-His Tag CDS 985..2667 /label=fadD acyl-CoA synthetase CDS 985..2694 /label=BBa_K654059 stem_loop 2714..2757 /label=stem_loop misc_feature 2703..2782 /label=BBa_B0010 terminator 2703..2782 /label=BBa_B0010 stem_loop 2798..2817 /label=T7 TE misc_feature 2824..2824 /label=stop polyA_site 2818..2831 /label=polya misc_feature 2791..2831 /label=BBa_B0012 terminator 2791..2831 /label=BBa_B0012 terminator 2703..2831 /label=BBa_B0015 misc_feature 985..2831 /label=BBa_K654065 misc_feature 890..2831 /label=BBa_K654071 ORIGIN 1 gctttacaaa actctcatta atcctttaga ctaagtttag tcagttccaa tctgaacatc 61 gacaaataca taaggaatta taaccaatac tagatgatga acttcaacaa tgttttccgc 121 tggcatttgc ccttcctgtt cctggtcctg ttaaccttcc gtgccgccgc agcggacacg 181 ttattgattc tgggtgatag cctgagcgcc gggtatcgaa tgtctgccag cgcggcctgg 241 cctgccttgt tgaatgataa gtggcagagt aaaacgtcgg tagttaatgc cagcatcagc 301 ggcgacacct cgcaacaagg actggcgcgc cttccggctc tgctgaaaca gcatcagccg 361 cgttgggtgc tggttgaact gggcggcaat gacggtttgc gtggttttca gccacagcaa 421 accgagcaaa cgctgcgcca gattttgcag gatgtcaaag ccgccaacgc tgaaccattg 481 ttaatgcaaa tacgtctgcc tgcaaactat ggtcgccgtt ataatgaagc ctttagcgcc 541 atttacccca aactcgccaa agagtttgat gttccgctgc tgcccttttt tatggaagag 601 gtctacctca agccacaatg gatgcaggat gacggtattc atcccaaccg cgacgcccag 661 ccgtttattg ccgactggat ggcgaagcag ttgcagcctt tagtaaatca tgactcacat 721 catcaccatc atcaccatca ctaatactag agccaggcat caaataaaac gaaaggctca 781 gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tctactagag 841 tcacactggc tcaccttcgg gtgggccttt ctgcgtttat atactagagg ctttacaaaa 901 ctctcattaa tcctttagac taagtttagt cagttccaat ctgaacatcg acaaatacat 961 aaggaattat aaccaatact agagttgaag aaggtttggc ttaaccgtta tcccgcggac 1021 gttccgacgg agatcaaccc tgaccgttat caatctctgg tagatatgtt tgagcagtcg 1081 gtcgcgcgct acgccgatca acctgcgttt gtgaatatgg gggaggtaat gaccttccgc 1141 aagctggaag aacgcagtcg cgcgtttgcc gcttatttgc aacaagggtt ggggctgaag 1201 aaaggcgatc gcgttgcgtt gatgatgcct aatttattgc aatatccggt ggcgctgttt 1261 ggcattttgc gtgccgggat gatcgtcgta aacgttaacc cgttgtatac cccgcgtgag 1321 cttgagcatc agcttaacga tagcggcgca tcggcgattg ttatcgtgtc taactttgct 1381 cacacactgg aaaaagtggt tgataaaacc gccgttcagc acgtaattct gacccgtatg 1441 ggcgatcagc tatctacggc aaaaggcacg gtagtcaatt tcgttgttaa atacatcaag 1501 cgtttggtgc cgaaatacca tctgccagat gccatttcat ttcgtagcgc actgcataac 1561 ggctaccgga tgcagtacgt caaacccgaa ctggtgccgg aagatttagc ttttctgcaa 1621 tacaccggcg gcaccactgg tgtggcgaaa ggcgcgatgc tgactcaccg caatatgctg 1681 gcgaacctgg aacaggttaa cgcgacctat ggtccgctgt tgcatccggg caaagagctg 1741 gtggtgacgg cgctgccgct gtatcacatt tttgccctga ccattaactg cctgctgttt 1801 atcgaactgg gtgggcagaa cctgcttatc actaacccgc gcgatattcc agggttggta 1861 aaagagttag cgaaatatcc gtttaccgct atcacgggcg ttaacacctt gttcaatgcg 1921 ttgctgaaca ataaagagtt ccagcagctg gatttctcca gtctgcatct ttccgcaggc 1981 ggtgggatgc cagtgcagca agtggtggca gagcgttggg tgaaactgac cggacagtat 2041 ctgctggaag gctatggcct taccgagtgt gcgccgctgg tcagcgttaa cccatatgat 2101 attgattatc atagtggtag catcggtttg ccggtgccgt cgacggaagc caaactggtg 2161 gatgatgatg ataatgaagt accaccaggt caaccgggtg agctttgtgt caaaggaccg 2221 caggtgatgc tgggttactg gcagcgtccc gatgctaccg atgaaatcat caaaaatggc 2281 tggttacaca ccggcgacat cgcggtaatg gatgaagaag gattcctgcg cattgtcgat 2341 cgtaaaaaag acatgattct ggtttccggt tttaacgtct atcccaacga gattgaagat 2401 gtcgtcatgc agcatcctgg cgtacaggaa gtcgcggctg ttggcgtacc ttccggctcc 2461 agtggtgaag cggtgaaaat cttcgtagtg aaaaaagatc catcgcttac cgaagagtca 2521 ctggtgactt tttgccgccg tcagctcacg ggatacaaag taccgaagct ggtggagttt 2581 cgtgatgagt taccgaaatc taacgtcgga aaaattttgc gacgagaatt acgtgacgaa 2641 gcgcgcggca aagtggacaa taaagcccat catcaccatc atcaccatca ctgatactag 2701 agccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg 2761 ttgtttgtcg gtgaacgctc tctactagag tcacactggc tcaccttcgg gtgggccttt 2821 ctgcgtttat a //