LOCUS       BBa_K654084             2235 bp    DNA     linear       25-Nov-2024
DEFINITION  psbA2 Controlled Fatty ACP Reductase + Fatty Aldehyde Decarbonylase
ACCESSION   BBa_K654084
VERSION     BBa_K654084.1  
FEATURES             Location/Qualifiers
     promoter        1..38
                     /label=psbA2 promoter
     RBS             71..80
                     /label=putative rbs
     misc_feature    3..8
                     /label=-35 box
     misc_feature    27..32
                     /label=-10 box
     misc_feature    39..39
                     /label=+1 Transcription Start Point
     promoter        1..87
                     /label=BBa_K390017
     misc_feature    94..96
                     /label=Start
     misc_feature    1138..1140
                     /label=Stop
     CDS             94..1113
                     /label=Fatty Acyl ACP Reductase
     misc_feature    1114..1137
                     /label=8-His Tag
     CDS             94..1140
                     /label=BBa_K654062
     stem_loop       1160..1203
                     /label=stem_loop
     misc_feature    1149..1228
                     /label=BBa_B0010
     terminator      1149..1228
                     /label=BBa_B0010
     stem_loop       1244..1263
                     /label=T7 TE
     misc_feature    1270..1270
                     /label=stop
     polyA_site      1264..1277
                     /label=polya
     misc_feature    1237..1277
                     /label=BBa_B0012
     terminator      1237..1277
                     /label=BBa_B0012
     terminator      1149..1277
                     /label=BBa_B0015
     misc_feature    94..1277
                     /label=BBa_K654068
     misc_feature    1..1277
                     /label=BBa_K654074
     promoter        1286..1323
                     /label=psbA2 promoter
     RBS             1356..1365
                     /label=putative rbs
     misc_feature    1288..1293
                     /label=-35 box
     misc_feature    1312..1317
                     /label=-10 box
     misc_feature    1324..1324
                     /label=+1 Transcription Start Point
     promoter        1286..1372
                     /label=BBa_K390017
     misc_feature    1379..1381
                     /label=Start
     misc_feature    2096..2098
                     /label=Stop
     misc_feature    2072..2095
                     /label=8-His Tag
     CDS             1379..2071
                     /label=Fatty Aldehyde Decarbonylase 
     CDS             1379..2098
                     /label=BBa_K654063
     stem_loop       2118..2161
                     /label=stem_loop
     misc_feature    2107..2186
                     /label=BBa_B0010
     terminator      2107..2186
                     /label=BBa_B0010
     stem_loop       2202..2221
                     /label=T7 TE
     misc_feature    2228..2228
                     /label=stop
     polyA_site      2222..2235
                     /label=polya
     misc_feature    2195..2235
                     /label=BBa_B0012
     terminator      2195..2235
                     /label=BBa_B0012
     terminator      2107..2235
                     /label=BBa_B0015
     misc_feature    1379..2235
                     /label=BBa_K654069
     misc_feature    1286..2235
                     /label=BBa_K654075
ORIGIN
        1 gctttacaaa actctcatta atcctttaga ctaagtttag tcagttccaa tctgaacatc
       61 gacaaataca taaggaatta taaccaatac tagatgtttg gtcttattgg tcatctcacg
      121 agtttagaac acgcccaagc ggttgctgaa gatttaggct atcctgagta cgccaaccaa
      181 ggcctggatt tttggtgttc ggctcctccc caagtggttg ataattttca ggtgaaaagt
      241 gtgacggggc aggtgattga aggcaaatat gtggagtctt gctttttgcc ggaaatgtta
      301 acccaacggc ggatcaaagc ggccattcgt aaaatcctca atgctatggc cctggcccaa
      361 aaggtgggct tggatattac ggccctggga ggcttttctt caatcgtatt tgaagaattt
      421 aacctcaagc aaaataatca agtccgcaat gtggaactag attttcagcg gttcaccact
      481 ggtaataccc acaccgctta tgtgatctgc cgtcaggtcg agtctggagc taaacagttg
      541 ggtattgatc taagtcaggc aacggtagcg gtttgtggcg ccacgggaga tattggtagc
      601 gccgtatgtc gttggttaga tagcaaacat caagttaagg aattattgct aattgcccgt
      661 aaccgccaaa gattggaaaa tctccaagag gaattgggtc ggggcaaaat tatggatttg
      721 gaaacagccc tgccccaggc agatattatt gtttgggtgg ctagtatgcc caagggggta
      781 gaaattgcgg gggaaatgct gaaaaagccc tgtttgattg tggatggggg ctatcccaag
      841 aatttagaca ccagggtgaa agcggatggg gtgcatattc tcaagggggg gattgtagaa
      901 cattcccttg atattacctg ggaaattatg aagattgtgg agatggatat tccctcccgg
      961 caaatgttcg cctgttttgc ggaggccatt ttgctagagt ttgagggctg gcgcactaat
     1021 ttttcctggg gccgcaacca aatttccgtt aataaaatgg aggcgattgg tgaagcttct
     1081 gtcaagcatg gcttttgccc tttagtagct cttcatcatc accatcatca ccatcactag
     1141 tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt
     1201 tatctgttgt ttgtcggtga acgctctcta ctagagtcac actggctcac cttcgggtgg
     1261 gcctttctgc gtttatatac tagaggcttt acaaaactct cattaatcct ttagactaag
     1321 tttagtcagt tccaatctga acatcgacaa atacataagg aattataacc aatactagat
     1381 gcccgagctt gctgtccgca ccgaatttga ctattccagc gaaatttaca aagacgccta
     1441 tagccgcatc aacgccattg tcattgaagg cgaacaggaa gcctacagca actacctcca
     1501 gatggcggaa ctcttgccgg aagacaaaga agagttgacc cgcttggcca aaatggaaaa
     1561 ccgccataaa aaaggtttcc aagcctgtgg caacaacctc caagtgaacc ctgatatgcc
     1621 ctatgcccag gaatttttcg ccggtctcca tggcaatttc cagcacgctt ttagcgaagg
     1681 gaaagttgtt acctgtttat tgatccaggc tttgattatc gaagcttttg cgatcgccgc
     1741 ctataacata tatatccctg tggcggacga ctttgctcgg aaaatcactg agggcgtagt
     1801 caaggacgaa tacacccacc tcaactacgg ggaagaatgg ctaaaggcca actttgccac
     1861 cgctaaggaa gaactggagc aggccaacaa agaaaaccta cccttagtgt ggaaaatgct
     1921 caaccaagtg cagggggacg ccaaggtatt gggcatggaa aaagaagccc tagtggaaga
     1981 ttttatgatc agctacggcg aagccctcag taacatcggc ttcagcacca gggaaattat
     2041 gcgtatgtct tcctacggtt tggccggagt ccatcatcac catcatcacc atcactagta
     2101 ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta
     2161 tctgttgttt gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc
     2221 ctttctgcgt ttata
//