BBa_K654087
type: ComponentDefinition
persistent identity: BBa K654087
display id: BBa_K654087
version: 1
was generated by: iGEM to SBOL conversion
description: sigA controlled Thioesterase + Acyl-CoA Synthetase
created: 2011-09-26T11:00:00Z
modified: 2015-05-08T01:13:02Z
mutable provenance: existing parts
owned by: james
owned by: myers
mutable description: to be added
discontinued: false
dominant: false
group u list: _831_
m user id: 0
owner id: 10131
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: none
star: false
creator: Charles Barentine
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K654076
component: BBa_K654077
sequence annotation: BBa_K654076
sequence annotation: BBa_K654077
sequence: https://synbiohub.org/public/igem/BBa_K654087_sequence/1

BBa_K654076
type: Component
persistent identity: component2143470
display id: component2143470
version: 1
top level: BBa_K654087
definition: BBa_K654076
access: public

BBa_K654077
type: Component
persistent identity: component2143490
display id: component2143490
version: 1
top level: BBa_K654087
definition: BBa_K654077
access: public

BBa_K654076
type: SequenceAnnotation
persistent identity: annotation2143470
display id: annotation2143470
version: 1
top level: BBa_K654087
location: https://synbiohub.org/public/igem/BBa_K654087/annotation2143470/range2143470/1
component: BBa_K654076

https://synbiohub.org/public/igem/BBa_K654087/annotation2143470/range2143470/1
type: Range
persistent identity: range2143470
display id: range2143470
version: 1
top level: BBa_K654087
direction: direction/reverse
start: 1
end: 855
orientation: inline

BBa_K654077
type: SequenceAnnotation
persistent identity: annotation2143490
display id: annotation2143490
version: 1
top level: BBa_K654087
location: https://synbiohub.org/public/igem/BBa_K654087/annotation2143490/range2143490/1
component: BBa_K654077

https://synbiohub.org/public/igem/BBa_K654087/annotation2143490/range2143490/1
type: Range
persistent identity: range2143490
display id: range2143490
version: 1
top level: BBa_K654087
direction: direction/reverse
start: 864
end: 2779
orientation: inline

BBa_K654076
type: ComponentDefinition
persistent identity: BBa K654076
display id: BBa_K654076
version: 1
was generated by: iGEM to SBOL conversion
description: sigA controlled Thioesterase
created: 2011-09-25T11:00:00Z
modified: 2015-05-08T01:13:01Z
mutable provenance: existing parts
owned by: james
owned by: myers
mutable description: to be added
discontinued: false
dominant: false
group u list: _831_
m user id: 0
owner id: 8952
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: none
star: false
creator: Joshua Ellis
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_K654064
component: BBa_K390018
sequence annotation: BBa_K390018
sequence annotation: BBa_K654064
sequence: https://synbiohub.org/public/igem/BBa_K654076_sequence/1

BBa_K654064
type: Component
persistent identity: component2143252
display id: component2143252
version: 1
top level: BBa_K654076
definition: BBa_K654064
access: public

BBa_K390018
type: Component
persistent identity: component2143239
display id: component2143239
version: 1
top level: BBa_K654076
definition: BBa_K390018
access: public

BBa_K390018
type: SequenceAnnotation
persistent identity: annotation2143239
display id: annotation2143239
version: 1
top level: BBa_K654076
location: https://synbiohub.org/public/igem/BBa_K654076/annotation2143239/range2143239/1
component: BBa_K390018

https://synbiohub.org/public/igem/BBa_K654076/annotation2143239/range2143239/1
type: Range
persistent identity: range2143239
display id: range2143239
version: 1
top level: BBa_K654076
direction: direction/reverse
start: 1
end: 61
orientation: inline

BBa_K654064
type: SequenceAnnotation
persistent identity: annotation2143252
display id: annotation2143252
version: 1
top level: BBa_K654076
location: https://synbiohub.org/public/igem/BBa_K654076/annotation2143252/range2143252/1
component: BBa_K654064

https://synbiohub.org/public/igem/BBa_K654076/annotation2143252/range2143252/1
type: Range
persistent identity: range2143252
display id: range2143252
version: 1
top level: BBa_K654076
direction: direction/reverse
start: 68
end: 855
orientation: inline

BBa_K654077
type: ComponentDefinition
persistent identity: BBa K654077
display id: BBa_K654077
version: 1
was generated by: iGEM to SBOL conversion
description: sigA controlled Fatty Acyl-CoA Synthetase (Ligase)
created: 2011-09-25T11:00:00Z
modified: 2015-05-08T01:13:01Z
mutable provenance: existing parts
owned by: james
owned by: myers
mutable description: to be added
discontinued: false
dominant: false
group u list: _831_
m user id: 0
owner id: 10131
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: none
star: false
creator: Charles Barentine
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_K654065
component: BBa_K390018
sequence annotation: BBa_K390018
sequence annotation: BBa_K654065
sequence: https://synbiohub.org/public/igem/BBa_K654077_sequence/1

BBa_K654065
type: Component
persistent identity: component2143271
display id: component2143271
version: 1
top level: BBa_K654077
definition: BBa_K654065
access: public

BBa_K390018
type: Component
persistent identity: component2143258
display id: component2143258
version: 1
top level: BBa_K654077
definition: BBa_K390018
access: public

BBa_K390018
type: SequenceAnnotation
persistent identity: annotation2143258
display id: annotation2143258
version: 1
top level: BBa_K654077
location: https://synbiohub.org/public/igem/BBa_K654077/annotation2143258/range2143258/1
component: BBa_K390018

https://synbiohub.org/public/igem/BBa_K654077/annotation2143258/range2143258/1
type: Range
persistent identity: range2143258
display id: range2143258
version: 1
top level: BBa_K654077
direction: direction/reverse
start: 1
end: 61
orientation: inline

BBa_K654065
type: SequenceAnnotation
persistent identity: annotation2143271
display id: annotation2143271
version: 1
top level: BBa_K654077
location: https://synbiohub.org/public/igem/BBa_K654077/annotation2143271/range2143271/1
component: BBa_K654065

https://synbiohub.org/public/igem/BBa_K654077/annotation2143271/range2143271/1
type: Range
persistent identity: range2143271
display id: range2143271
version: 1
top level: BBa_K654077
direction: direction/reverse
start: 70
end: 1916
orientation: inline

BBa_K654064
type: ComponentDefinition
persistent identity: BBa K654064
display id: BBa_K654064
version: 1
was generated by: iGEM to SBOL conversion
description: Thioesterase + Terminator
created: 2011-09-25T11:00:00Z
modified: 2015-05-08T01:13:01Z
mutable provenance: To be added
owned by: james
owned by: myers
mutable description: To be added
discontinued: false
dominant: false
group u list: _831_
m user id: 0
owner id: 8952
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: To be added
star: false
creator: Joshua Ellis
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0015
component: BBa_K654058
sequence annotation: BBa_K654058
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_K654064_sequence/1

BBa_B0015
type: Component
persistent identity: component2143077
display id: component2143077
version: 1
top level: BBa_K654064
definition: BBa_B0015
access: public

BBa_K654058
type: Component
persistent identity: component2143070
display id: component2143070
version: 1
top level: BBa_K654064
definition: BBa_K654058
access: public

BBa_K654058
type: SequenceAnnotation
persistent identity: annotation2143070
display id: annotation2143070
version: 1
top level: BBa_K654064
location: https://synbiohub.org/public/igem/BBa_K654064/annotation2143070/range2143070/1
component: BBa_K654058

https://synbiohub.org/public/igem/BBa_K654064/annotation2143070/range2143070/1
type: Range
persistent identity: range2143070
display id: range2143070
version: 1
top level: BBa_K654064
direction: direction/reverse
start: 1
end: 651
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2143077
display id: annotation2143077
version: 1
top level: BBa_K654064
location: https://synbiohub.org/public/igem/BBa_K654064/annotation2143077/range2143077/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K654064/annotation2143077/range2143077/1
type: Range
persistent identity: range2143077
display id: range2143077
version: 1
top level: BBa_K654064
direction: direction/reverse
start: 660
end: 788
orientation: inline

BBa_K654065
type: ComponentDefinition
persistent identity: BBa K654065
display id: BBa_K654065
version: 1
was generated by: iGEM to SBOL conversion
description: Fatty Acyl-CoA Synthetase (Ligase) + Terminator
created: 2011-09-25T11:00:00Z
modified: 2015-05-08T01:13:01Z
mutable provenance: To be added
owned by: james
owned by: myers
mutable description: To be added
discontinued: false
dominant: false
group u list: _831_
m user id: 0
owner id: 8952
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: To be added
star: false
creator: Joshua Ellis
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_B0015
component: BBa_K654059
sequence annotation: BBa_K654059
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_K654065_sequence/1

BBa_B0015
type: Component
persistent identity: component2143089
display id: component2143089
version: 1
top level: BBa_K654065
definition: BBa_B0015
access: public

BBa_K654059
type: Component
persistent identity: component2143082
display id: component2143082
version: 1
top level: BBa_K654065
definition: BBa_K654059
access: public

BBa_K654059
type: SequenceAnnotation
persistent identity: annotation2143082
display id: annotation2143082
version: 1
top level: BBa_K654065
location: https://synbiohub.org/public/igem/BBa_K654065/annotation2143082/range2143082/1
component: BBa_K654059

https://synbiohub.org/public/igem/BBa_K654065/annotation2143082/range2143082/1
type: Range
persistent identity: range2143082
display id: range2143082
version: 1
top level: BBa_K654065
direction: direction/reverse
start: 1
end: 1710
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2143089
display id: annotation2143089
version: 1
top level: BBa_K654065
location: https://synbiohub.org/public/igem/BBa_K654065/annotation2143089/range2143089/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K654065/annotation2143089/range2143089/1
type: Range
persistent identity: range2143089
display id: range2143089
version: 1
top level: BBa_K654065
direction: direction/reverse
start: 1719
end: 1847
orientation: inline

BBa_K390018
type: ComponentDefinition
persistent identity: BBa K390018
display id: BBa_K390018
version: 1
was generated by: iGEM to SBOL conversion
description: sigA promoter, 5'UTR, and RBS
created: 2010-08-01T11:00:00Z
modified: 2015-05-08T01:12:19Z
mutable provenance: (coming soon)
owned by: james
owned by: myers
mutable description: (coming soon)
discontinued: false
dominant: false
group u list: _518_
m user id: 0
owner id: 6788
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: (coming soon)
star: false
creator: Cody Tramp
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: sigA promoter
sequence annotation: -35 box
sequence annotation: -10 box
sequence annotation: putative rbs
sequence annotation: +1 Transcription Start Point
sequence: https://synbiohub.org/public/igem/BBa_K390018_sequence/1

sigA promoter
type: SequenceAnnotation
persistent identity: annotation2076608
display id: annotation2076608
version: 1
top level: BBa_K390018
location: https://synbiohub.org/public/igem/BBa_K390018/annotation2076608/range2076608/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_K390018/annotation2076608/range2076608/1
type: Range
persistent identity: range2076608
display id: range2076608
version: 1
top level: BBa_K390018
direction: direction/forward
start: 4
end: 45
orientation: inline

-35 box
type: SequenceAnnotation
persistent identity: annotation2076611
display id: annotation2076611
version: 1
top level: BBa_K390018
location: https://synbiohub.org/public/igem/BBa_K390018/annotation2076611/range2076611/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K390018/annotation2076611/range2076611/1
type: Range
persistent identity: range2076611
display id: range2076611
version: 1
top level: BBa_K390018
direction: direction/forward
start: 9
end: 14
orientation: inline

-10 box
type: SequenceAnnotation
persistent identity: annotation2076612
display id: annotation2076612
version: 1
top level: BBa_K390018
location: https://synbiohub.org/public/igem/BBa_K390018/annotation2076612/range2076612/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_K390018/annotation2076612/range2076612/1
type: Range
persistent identity: range2076612
display id: range2076612
version: 1
top level: BBa_K390018
direction: direction/forward
start: 30
end: 35
orientation: inline

putative rbs
type: SequenceAnnotation
persistent identity: annotation2076609
display id: annotation2076609
version: 1
top level: BBa_K390018
location: https://synbiohub.org/public/igem/BBa_K390018/annotation2076609/range2076609/1
role: SO:0000139
role: feature/rbs

https://synbiohub.org/public/igem/BBa_K390018/annotation2076609/range2076609/1
type: Range
persistent identity: range2076609
display id: range2076609
version: 1
top level: BBa_K390018
direction: direction/forward
start: 46
end: 55
orientation: inline

+1 Transcription Start Point
type: SequenceAnnotation
persistent identity: annotation2076610
display id: annotation2076610
version: 1
top level: BBa_K390018
location: https://synbiohub.org/public/igem/BBa_K390018/annotation2076610/range2076610/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K390018/annotation2076610/range2076610/1
type: Range
persistent identity: range2076610
display id: range2076610
version: 1
top level: BBa_K390018
direction: direction/forward
start: 46
end: 46
orientation: inline

BBa_B0015
type: ComponentDefinition
persistent identity: BBa B0015
display id: BBa_B0015
version: 1
was generated by: iGEM to SBOL conversion
description: double terminator (B0010-B0012)
created: 2003-07-16T11:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Double terminator consisting of BBa_B0010 and BBa_B0012
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Reshma Shetty
type: DnaRegion
role: partType/Terminator
role: SO:0000141
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_B0015_sequence/1

BBa_B0012
type: Component
persistent identity: component1916612
display id: component1916612
version: 1
top level: BBa_B0015
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1916610
display id: component1916610
version: 1
top level: BBa_B0015
definition: BBa_B0010
access: public

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1916610
display id: annotation1916610
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
type: Range
persistent identity: range1916610
display id: range1916610
version: 1
top level: BBa_B0015
direction: direction/forward
start: 1
end: 80
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1916612
display id: annotation1916612
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
type: Range
persistent identity: range1916612
display id: range1916612
version: 1
top level: BBa_B0015
direction: direction/forward
start: 89
end: 129
orientation: inline

BBa_K654058
type: ComponentDefinition
persistent identity: BBa K654058
display id: BBa_K654058
version: 1
was generated by: iGEM to SBOL conversion
description: Thioesterase (TesA from E. coli) with 8-His Tag
created: 2011-08-11T11:00:00Z
modified: 2015-05-08T01:13:01Z
mutable provenance: E. coli genomic DNA
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: TesA thioesterase gene from E. coli. Contains a 8-Histidine tag immediately before the stop codon to allow gel identification and extraction.
discontinued: false
dominant: false
experience: experience/None
group u list: _831_
m user id: 0
owner id: 6788
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable notes: 8-His tag added just before the stop codon - C terminus end of the protein. May alter activity level.
star: false
creator: Cody Tramp
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Start
sequence annotation: Thioesterase (TesA)
sequence annotation: 8-His Tag
sequence annotation: Stop
sequence: https://synbiohub.org/public/igem/BBa_K654058_sequence/1

Start
type: SequenceAnnotation
persistent identity: annotation2124921
display id: annotation2124921
version: 1
top level: BBa_K654058
location: https://synbiohub.org/public/igem/BBa_K654058/annotation2124921/range2124921/1
role: SO:0000318
role: feature/start

https://synbiohub.org/public/igem/BBa_K654058/annotation2124921/range2124921/1
type: Range
persistent identity: range2124921
display id: range2124921
version: 1
top level: BBa_K654058
direction: direction/forward
start: 1
end: 3
orientation: inline

Thioesterase (TesA)
type: SequenceAnnotation
persistent identity: annotation2124924
display id: annotation2124924
version: 1
top level: BBa_K654058
location: https://synbiohub.org/public/igem/BBa_K654058/annotation2124924/range2124924/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_K654058/annotation2124924/range2124924/1
type: Range
persistent identity: range2124924
display id: range2124924
version: 1
top level: BBa_K654058
direction: direction/forward
start: 1
end: 624
orientation: inline

8-His Tag
type: SequenceAnnotation
persistent identity: annotation2124923
display id: annotation2124923
version: 1
top level: BBa_K654058
location: https://synbiohub.org/public/igem/BBa_K654058/annotation2124923/range2124923/1
role: SO:0000324
role: feature/tag

https://synbiohub.org/public/igem/BBa_K654058/annotation2124923/range2124923/1
type: Range
persistent identity: range2124923
display id: range2124923
version: 1
top level: BBa_K654058
direction: direction/forward
start: 625
end: 648
orientation: inline

Stop
type: SequenceAnnotation
persistent identity: annotation2124922
display id: annotation2124922
version: 1
top level: BBa_K654058
location: https://synbiohub.org/public/igem/BBa_K654058/annotation2124922/range2124922/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_K654058/annotation2124922/range2124922/1
type: Range
persistent identity: range2124922
display id: range2124922
version: 1
top level: BBa_K654058
direction: direction/forward
start: 649
end: 651
orientation: inline

BBa_K654059
type: ComponentDefinition
persistent identity: BBa K654059
display id: BBa_K654059
version: 1
was generated by: iGEM to SBOL conversion
description: Fatty Acyl-CoA Synthetase (Ligase) (fadD from E. coli) with 8-His Tag
created: 2011-09-25T11:00:00Z
modified: 2015-05-08T01:13:01Z
mutable provenance: E. coli genomic DNA
owned by: james
owned by: myers
mutable description: Fatty Acyl-CoA Synthetase (Ligase) (fadD from E. coli) with 8-His Tag
discontinued: false
dominant: false
group u list: _831_
m user id: 0
owner id: 6788
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: none
star: false
creator: Cody Tramp
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Start
sequence annotation: fadD acyl-CoA synthetase
sequence annotation: 8-His Tag
sequence annotation: Stop
sequence: https://synbiohub.org/public/igem/BBa_K654059_sequence/1

Start
type: SequenceAnnotation
persistent identity: annotation2141916
display id: annotation2141916
version: 1
top level: BBa_K654059
location: https://synbiohub.org/public/igem/BBa_K654059/annotation2141916/range2141916/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_K654059/annotation2141916/range2141916/1
type: Range
persistent identity: range2141916
display id: range2141916
version: 1
top level: BBa_K654059
direction: direction/forward
start: 1
end: 3
orientation: inline

fadD acyl-CoA synthetase
type: SequenceAnnotation
persistent identity: annotation2141919
display id: annotation2141919
version: 1
top level: BBa_K654059
location: https://synbiohub.org/public/igem/BBa_K654059/annotation2141919/range2141919/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_K654059/annotation2141919/range2141919/1
type: Range
persistent identity: range2141919
display id: range2141919
version: 1
top level: BBa_K654059
direction: direction/forward
start: 1
end: 1683
orientation: inline

8-His Tag
type: SequenceAnnotation
persistent identity: annotation2141918
display id: annotation2141918
version: 1
top level: BBa_K654059
location: https://synbiohub.org/public/igem/BBa_K654059/annotation2141918/range2141918/1
role: feature/tag
role: SO:0000324

https://synbiohub.org/public/igem/BBa_K654059/annotation2141918/range2141918/1
type: Range
persistent identity: range2141918
display id: range2141918
version: 1
top level: BBa_K654059
direction: direction/forward
start: 1684
end: 1707
orientation: inline

Stop
type: SequenceAnnotation
persistent identity: annotation2141917
display id: annotation2141917
version: 1
top level: BBa_K654059
location: https://synbiohub.org/public/igem/BBa_K654059/annotation2141917/range2141917/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_K654059/annotation2141917/range2141917/1
type: Range
persistent identity: range2141917
display id: range2141917
version: 1
top level: BBa_K654059
direction: direction/forward
start: 1708
end: 1710
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: SO:0000553
role: feature/polya

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

https://synbiohub.org/public/igem/BBa_K654087_sequence/1
type: Sequence
persistent identity: BBa K654087 sequence
display id: BBa_K654087_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: cctcaactttgactgaccagctaattttgtacacgacttaggagttaacgaggaaaacggctactagatgatgaacttcaacaatgttttccgctggcatttgcccttcctgttcctggtcctgttaaccttccgtgccgccgcagcggacacgttattgattctgggtgatagcctgagcgccgggtatcgaatgtctgccagcgcggcctggcctgccttgttgaatgataagtggcagagtaaaacgtcggtagttaatgccagcatcagcggcgacacctcgcaacaaggactggcgcgccttccggctctgctgaaacagcatcagccgcgttgggtgctggttgaactgggcggcaatgacggtttgcgtggttttcagccacagcaaaccgagcaaacgctgcgccagattttgcaggatgtcaaagccgccaacgctgaaccattgttaatgcaaatacgtctgcctgcaaactatggtcgccgttataatgaagcctttagcgccatttaccccaaactcgccaaagagtttgatgttccgctgctgcccttttttatggaagaggtctacctcaagccacaatggatgcaggatgacggtattcatcccaaccgcgacgcccagccgtttattgccgactggatggcgaagcagttgcagcctttagtaaatcatgactcacatcatcaccatcatcaccatcactaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagcctcaactttgactgaccagctaattttgtacacgacttaggagttaacgaggaaaacggctactagagttgaagaaggtttggcttaaccgttatcccgcggacgttccgacggagatcaaccctgaccgttatcaatctctggtagatatgtttgagcagtcggtcgcgcgctacgccgatcaacctgcgtttgtgaatatgggggaggtaatgaccttccgcaagctggaagaacgcagtcgcgcgtttgccgcttatttgcaacaagggttggggctgaagaaaggcgatcgcgttgcgttgatgatgcctaatttattgcaatatccggtggcgctgtttggcattttgcgtgccgggatgatcgtcgtaaacgttaacccgttgtataccccgcgtgagcttgagcatcagcttaacgatagcggcgcatcggcgattgttatcgtgtctaactttgctcacacactggaaaaagtggttgataaaaccgccgttcagcacgtaattctgacccgtatgggcgatcagctatctacggcaaaaggcacggtagtcaatttcgttgttaaatacatcaagcgtttggtgccgaaataccatctgccagatgccatttcatttcgtagcgcactgcataacggctaccggatgcagtacgtcaaacccgaactggtgccggaagatttagcttttctgcaatacaccggcggcaccactggtgtggcgaaaggcgcgatgctgactcaccgcaatatgctggcgaacctggaacaggttaacgcgacctatggtccgctgttgcatccgggcaaagagctggtggtgacggcgctgccgctgtatcacatttttgccctgaccattaactgcctgctgtttatcgaactgggtgggcagaacctgcttatcactaacccgcgcgatattccagggttggtaaaagagttagcgaaatatccgtttaccgctatcacgggcgttaacaccttgttcaatgcgttgctgaacaataaagagttccagcagctggatttctccagtctgcatctttccgcaggcggtgggatgccagtgcagcaagtggtggcagagcgttgggtgaaactgaccggacagtatctgctggaaggc…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K654076_sequence/1
type: Sequence
persistent identity: BBa K654076 sequence
display id: BBa_K654076_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: cctcaactttgactgaccagctaattttgtacacgacttaggagttaacgaggaaaacggctactagatgatgaacttcaacaatgttttccgctggcatttgcccttcctgttcctggtcctgttaaccttccgtgccgccgcagcggacacgttattgattctgggtgatagcctgagcgccgggtatcgaatgtctgccagcgcggcctggcctgccttgttgaatgataagtggcagagtaaaacgtcggtagttaatgccagcatcagcggcgacacctcgcaacaaggactggcgcgccttccggctctgctgaaacagcatcagccgcgttgggtgctggttgaactgggcggcaatgacggtttgcgtggttttcagccacagcaaaccgagcaaacgctgcgccagattttgcaggatgtcaaagccgccaacgctgaaccattgttaatgcaaatacgtctgcctgcaaactatggtcgccgttataatgaagcctttagcgccatttaccccaaactcgccaaagagtttgatgttccgctgctgcccttttttatggaagaggtctacctcaagccacaatggatgcaggatgacggtattcatcccaaccgcgacgcccagccgtttattgccgactggatggcgaagcagttgcagcctttagtaaatcatgactcacatcatcaccatcatcaccatcactaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K654077_sequence/1
type: Sequence
persistent identity: BBa K654077 sequence
display id: BBa_K654077_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: cctcaactttgactgaccagctaattttgtacacgacttaggagttaacgaggaaaacggctactagagttgaagaaggtttggcttaaccgttatcccgcggacgttccgacggagatcaaccctgaccgttatcaatctctggtagatatgtttgagcagtcggtcgcgcgctacgccgatcaacctgcgtttgtgaatatgggggaggtaatgaccttccgcaagctggaagaacgcagtcgcgcgtttgccgcttatttgcaacaagggttggggctgaagaaaggcgatcgcgttgcgttgatgatgcctaatttattgcaatatccggtggcgctgtttggcattttgcgtgccgggatgatcgtcgtaaacgttaacccgttgtataccccgcgtgagcttgagcatcagcttaacgatagcggcgcatcggcgattgttatcgtgtctaactttgctcacacactggaaaaagtggttgataaaaccgccgttcagcacgtaattctgacccgtatgggcgatcagctatctacggcaaaaggcacggtagtcaatttcgttgttaaatacatcaagcgtttggtgccgaaataccatctgccagatgccatttcatttcgtagcgcactgcataacggctaccggatgcagtacgtcaaacccgaactggtgccggaagatttagcttttctgcaatacaccggcggcaccactggtgtggcgaaaggcgcgatgctgactcaccgcaatatgctggcgaacctggaacaggttaacgcgacctatggtccgctgttgcatccgggcaaagagctggtggtgacggcgctgccgctgtatcacatttttgccctgaccattaactgcctgctgtttatcgaactgggtgggcagaacctgcttatcactaacccgcgcgatattccagggttggtaaaagagttagcgaaatatccgtttaccgctatcacgggcgttaacaccttgttcaatgcgttgctgaacaataaagagttccagcagctggatttctccagtctgcatctttccgcaggcggtgggatgccagtgcagcaagtggtggcagagcgttgggtgaaactgaccggacagtatctgctggaaggctatggccttaccgagtgtgcgccgctggtcagcgttaacccatatgatattgattatcatagtggtagcatcggtttgccggtgccgtcgacggaagccaaactggtggatgatgatgataatgaagtaccaccaggtcaaccgggtgagctttgtgtcaaaggaccgcaggtgatgctgggttactggcagcgtcccgatgctaccgatgaaatcatcaaaaatggctggttacacaccggcgacatcgcggtaatggatgaagaaggattcctgcgcattgtcgatcgtaaaaaagacatgattctggtttccggttttaacgtctatcccaacgagattgaagatgtcgtcatgcagcatcctggcgtacaggaagtcgcggctgttggcgtaccttccggctccagtggtgaagcggtgaaaatcttcgtagtgaaaaaagatccatcgcttaccgaagagtcactggtgactttttgccgccgtcagctcacgggatacaaagtaccgaagctggtggagtttcgtgatgagttaccgaaatctaacgtcggaaaaattttgcgacgagaattacgtgacgaagcgcgcggcaaagtggacaataaagcccatcatcaccatcatcaccatcactgatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K654064_sequence/1
type: Sequence
persistent identity: BBa K654064 sequence
display id: BBa_K654064_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgatgaacttcaacaatgttttccgctggcatttgcccttcctgttcctggtcctgttaaccttccgtgccgccgcagcggacacgttattgattctgggtgatagcctgagcgccgggtatcgaatgtctgccagcgcggcctggcctgccttgttgaatgataagtggcagagtaaaacgtcggtagttaatgccagcatcagcggcgacacctcgcaacaaggactggcgcgccttccggctctgctgaaacagcatcagccgcgttgggtgctggttgaactgggcggcaatgacggtttgcgtggttttcagccacagcaaaccgagcaaacgctgcgccagattttgcaggatgtcaaagccgccaacgctgaaccattgttaatgcaaatacgtctgcctgcaaactatggtcgccgttataatgaagcctttagcgccatttaccccaaactcgccaaagagtttgatgttccgctgctgcccttttttatggaagaggtctacctcaagccacaatggatgcaggatgacggtattcatcccaaccgcgacgcccagccgtttattgccgactggatggcgaagcagttgcagcctttagtaaatcatgactcacatcatcaccatcatcaccatcactaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K654065_sequence/1
type: Sequence
persistent identity: BBa K654065 sequence
display id: BBa_K654065_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttgaagaaggtttggcttaaccgttatcccgcggacgttccgacggagatcaaccctgaccgttatcaatctctggtagatatgtttgagcagtcggtcgcgcgctacgccgatcaacctgcgtttgtgaatatgggggaggtaatgaccttccgcaagctggaagaacgcagtcgcgcgtttgccgcttatttgcaacaagggttggggctgaagaaaggcgatcgcgttgcgttgatgatgcctaatttattgcaatatccggtggcgctgtttggcattttgcgtgccgggatgatcgtcgtaaacgttaacccgttgtataccccgcgtgagcttgagcatcagcttaacgatagcggcgcatcggcgattgttatcgtgtctaactttgctcacacactggaaaaagtggttgataaaaccgccgttcagcacgtaattctgacccgtatgggcgatcagctatctacggcaaaaggcacggtagtcaatttcgttgttaaatacatcaagcgtttggtgccgaaataccatctgccagatgccatttcatttcgtagcgcactgcataacggctaccggatgcagtacgtcaaacccgaactggtgccggaagatttagcttttctgcaatacaccggcggcaccactggtgtggcgaaaggcgcgatgctgactcaccgcaatatgctggcgaacctggaacaggttaacgcgacctatggtccgctgttgcatccgggcaaagagctggtggtgacggcgctgccgctgtatcacatttttgccctgaccattaactgcctgctgtttatcgaactgggtgggcagaacctgcttatcactaacccgcgcgatattccagggttggtaaaagagttagcgaaatatccgtttaccgctatcacgggcgttaacaccttgttcaatgcgttgctgaacaataaagagttccagcagctggatttctccagtctgcatctttccgcaggcggtgggatgccagtgcagcaagtggtggcagagcgttgggtgaaactgaccggacagtatctgctggaaggctatggccttaccgagtgtgcgccgctggtcagcgttaacccatatgatattgattatcatagtggtagcatcggtttgccggtgccgtcgacggaagccaaactggtggatgatgatgataatgaagtaccaccaggtcaaccgggtgagctttgtgtcaaaggaccgcaggtgatgctgggttactggcagcgtcccgatgctaccgatgaaatcatcaaaaatggctggttacacaccggcgacatcgcggtaatggatgaagaaggattcctgcgcattgtcgatcgtaaaaaagacatgattctggtttccggttttaacgtctatcccaacgagattgaagatgtcgtcatgcagcatcctggcgtacaggaagtcgcggctgttggcgtaccttccggctccagtggtgaagcggtgaaaatcttcgtagtgaaaaaagatccatcgcttaccgaagagtcactggtgactttttgccgccgtcagctcacgggatacaaagtaccgaagctggtggagtttcgtgatgagttaccgaaatctaacgtcggaaaaattttgcgacgagaattacgtgacgaagcgcgcggcaaagtggacaataaagcccatcatcaccatcatcaccatcactgatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K390018_sequence/1
type: Sequence
persistent identity: BBa K390018 sequence
display id: BBa_K390018_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: cctcaactttgactgaccagctaattttgtacacgacttaggagttaacgaggaaaacggc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0015_sequence/1
type: Sequence
persistent identity: BBa B0015 sequence
display id: BBa_B0015_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K654058_sequence/1
type: Sequence
persistent identity: BBa K654058 sequence
display id: BBa_K654058_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgatgaacttcaacaatgttttccgctggcatttgcccttcctgttcctggtcctgttaaccttccgtgccgccgcagcggacacgttattgattctgggtgatagcctgagcgccgggtatcgaatgtctgccagcgcggcctggcctgccttgttgaatgataagtggcagagtaaaacgtcggtagttaatgccagcatcagcggcgacacctcgcaacaaggactggcgcgccttccggctctgctgaaacagcatcagccgcgttgggtgctggttgaactgggcggcaatgacggtttgcgtggttttcagccacagcaaaccgagcaaacgctgcgccagattttgcaggatgtcaaagccgccaacgctgaaccattgttaatgcaaatacgtctgcctgcaaactatggtcgccgttataatgaagcctttagcgccatttaccccaaactcgccaaagagtttgatgttccgctgctgcccttttttatggaagaggtctacctcaagccacaatggatgcaggatgacggtattcatcccaaccgcgacgcccagccgtttattgccgactggatggcgaagcagttgcagcctttagtaaatcatgactcacatcatcaccatcatcaccatcactaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K654059_sequence/1
type: Sequence
persistent identity: BBa K654059 sequence
display id: BBa_K654059_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttgaagaaggtttggcttaaccgttatcccgcggacgttccgacggagatcaaccctgaccgttatcaatctctggtagatatgtttgagcagtcggtcgcgcgctacgccgatcaacctgcgtttgtgaatatgggggaggtaatgaccttccgcaagctggaagaacgcagtcgcgcgtttgccgcttatttgcaacaagggttggggctgaagaaaggcgatcgcgttgcgttgatgatgcctaatttattgcaatatccggtggcgctgtttggcattttgcgtgccgggatgatcgtcgtaaacgttaacccgttgtataccccgcgtgagcttgagcatcagcttaacgatagcggcgcatcggcgattgttatcgtgtctaactttgctcacacactggaaaaagtggttgataaaaccgccgttcagcacgtaattctgacccgtatgggcgatcagctatctacggcaaaaggcacggtagtcaatttcgttgttaaatacatcaagcgtttggtgccgaaataccatctgccagatgccatttcatttcgtagcgcactgcataacggctaccggatgcagtacgtcaaacccgaactggtgccggaagatttagcttttctgcaatacaccggcggcaccactggtgtggcgaaaggcgcgatgctgactcaccgcaatatgctggcgaacctggaacaggttaacgcgacctatggtccgctgttgcatccgggcaaagagctggtggtgacggcgctgccgctgtatcacatttttgccctgaccattaactgcctgctgtttatcgaactgggtgggcagaacctgcttatcactaacccgcgcgatattccagggttggtaaaagagttagcgaaatatccgtttaccgctatcacgggcgttaacaccttgttcaatgcgttgctgaacaataaagagttccagcagctggatttctccagtctgcatctttccgcaggcggtgggatgccagtgcagcaagtggtggcagagcgttgggtgaaactgaccggacagtatctgctggaaggctatggccttaccgagtgtgcgccgctggtcagcgttaacccatatgatattgattatcatagtggtagcatcggtttgccggtgccgtcgacggaagccaaactggtggatgatgatgataatgaagtaccaccaggtcaaccgggtgagctttgtgtcaaaggaccgcaggtgatgctgggttactggcagcgtcccgatgctaccgatgaaatcatcaaaaatggctggttacacaccggcgacatcgcggtaatggatgaagaaggattcctgcgcattgtcgatcgtaaaaaagacatgattctggtttccggttttaacgtctatcccaacgagattgaagatgtcgtcatgcagcatcctggcgtacaggaagtcgcggctgttggcgtaccttccggctccagtggtgaagcggtgaaaatcttcgtagtgaaaaaagatccatcgcttaccgaagagtcactggtgactttttgccgccgtcagctcacgggatacaaagtaccgaagctggtggagtttcgtgatgagttaccgaaatctaacgtcggaaaaattttgcgacgagaattacgtgacgaagcgcgcggcaaagtggacaataaagcccatcatcaccatcatcaccatcactga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z