LOCUS       BBa_K654092             1091 bp    DNA     linear       22-Dec-2024
DEFINITION  E. coli 1.0 RBS + Renilla Luciferase + Terminator
ACCESSION   BBa_K654092
VERSION     BBa_K654092.1  
FEATURES             Location/Qualifiers
     misc_feature    5..8
                     /label=conserved
     RBS             1..12
                     /label=BBa_B0034
     misc_feature    19..954
                     /label=Rluc
     CDS             19..954
                     /label=Rluc
     misc_feature    19..954
                     /label=BBa_J52008
     stem_loop       974..1017
                     /label=stem_loop
     misc_feature    963..1042
                     /label=BBa_B0010
     terminator      963..1042
                     /label=BBa_B0010
     stem_loop       1058..1077
                     /label=T7 TE
     misc_feature    1084..1084
                     /label=stop
     polyA_site      1078..1091
                     /label=polya
     misc_feature    1051..1091
                     /label=BBa_B0012
     terminator      1051..1091
                     /label=BBa_B0012
     terminator      963..1091
                     /label=BBa_B0015
     misc_feature    19..1091
                     /label=BBa_K654034
ORIGIN
        1 aaagaggaga aatactagat ggcttccaag gtgtacgacc ccgagcaacg caaacgcatg
       61 atcactgggc ctcagtggtg ggctcgctgc aagcaaatga acgtgctgga ctccttcatc
      121 aactactatg attccgagaa gcacgccgag aacgccgtga tttttctgca tggtaacgct
      181 gcctccagct acctgtggag gcacgtcgtg cctcacatcg agcccgtggc tagatgcatc
      241 atccctgatc tgatcggaat gggtaagtcc ggcaagagcg ggaatggctc atatcgcctc
      301 ctggatcact acaagtacct caccgcttgg ttcgagctgc tgaaccttcc aaagaaaatc
      361 atctttgtgg gccacgactg gggggcttgt ctggcctttc actactccta cgagcaccaa
      421 gacaagatca aggccatcgt ccatgctgag agtgtcgtgg acgtgatcga gtcctgggac
      481 gagtggcctg acatcgagga ggatatcgcc ctgatcaaga gcgaagaggg cgagaaaatg
      541 gtgcttgaga ataacttctt cgtcgagacc atgctcccaa gcaagatcat gcggaaactg
      601 gagcctgagg agttcgctgc ctacctggag ccattcaagg agaagggcga ggttagacgg
      661 cctaccctct cctggcctcg cgagatccct ctcgttaagg gaggcaagcc cgacgtcgtc
      721 cagattgtcc gcaactacaa cgcctacctt cgggccagcg acgatctgcc taagatgttc
      781 atcgagtccg accctgggtt cttttccaac gctattgtcg agggagctaa gaagttccct
      841 aacaccgagt tcgtgaaggt gaagggcctc cacttcagcc aggaggacgc tccagatgaa
      901 atgggtaagt acatcaagag cttcgtggag cgcgtgctga agaacgagca gtaatactag
      961 agccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg
     1021 ttgtttgtcg gtgaacgctc tctactagag tcacactggc tcaccttcgg gtgggccttt
     1081 ctgcgtttat a
//