LOCUS BBa_K654998 5264 bp DNA linear 15-Jun-2024 DEFINITION tet HyBD and TolC ACCESSION BBa_K654998 VERSION BBa_K654998.1 FEATURES Location/Qualifiers misc_feature 1..19 /label=TetR 1 misc_feature 1..54 /label=BBa_R0040 promoter 20..25 /label=-35 misc_feature 26..44 /label=TetR 2 promoter 43..48 /label=-10 promoter 1..54 /label=BBa_R0040 RBS 63..76 /label=BBa_B0035 misc_feature 83..85 /label=Start Codon misc_feature 2204..2206 /label=Stop Codon CDS 83..2203 /label=HlyB CDS 83..2206 /label=BBa_M1003 RBS 2215..2228 /label=BBa_B0035 misc_feature 2235..2237 /label=Start Codon CDS 2235..3668 /label=HlyD misc_feature 3669..3671 /label=Stop Codon CDS 2235..3671 /label=BBa_M1004 RBS 3680..3693 /label=BBa_B0035 misc_feature 3700..3702 /label=Start misc_feature 5215..5217 /label=Stop CDS 3700..5214 /label=TolC CDS 3700..5217 /label=BBa_M1028 misc_feature 5226..5264 /label=B1006 stem_loop 5235..5256 /label=modified thr terminator polyA_site 5257..5264 /label=PolyA polyA_site 5226..5234 /label=PolyA terminator 5226..5264 /label=BBa_B1006 misc_feature 63..5264 /label=BBa_M1027 ORIGIN 1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag 61 agattaaaga ggagaatact agatggattc ctgtcacaag attgattacg gcctgtatgc 121 actggaaatc ctggcacaat atcataacgt ctccgtcaac ccggaggaaa ttaaacatcg 181 cttcgatact gacggcactg gcctgggtct gaccagctgg ctgctggcag cgaagtccct 241 ggagctgaag gtaaagcaag tgaaaaagac gattgatcgt ctgaacttca tcagcctgcc 301 ggcactggtt tggcgtgaag atggccgtca cttcatcctg acgaaagtgt ccaaagaagc 361 gaaccgctac ctgatctttg atctggagca gcgcaacccg cgcgtactgg aacagagcga 421 gttcgaggct ctgtatcagg gccacatcat tctgattgca tctcgttctt ctgttgcagg 481 taaactggcg aaattcgact tcacctggtt cattccggct attatcaagt accgtcgtat 541 tttcatcgaa accctggttg tatctgtttt cctgcaactg ttcgctctga ttactccgct 601 gttctttcag gttgtcatgg acaaagttct ggttcaccgt ggcttttcca ccctgaacgt 661 cattactgtt gcgctgagcg ttgttgttgt gttcgaaatc attctgtccg gtctgcgtac 721 ctacatcttc gcgcactcta cctcccgcat cgacgttgaa ctgggtgcga agctgttccg 781 tcacctgctg gcactgccta tcagctactt cgaatctcgt cgtgtgggcg atactgtggc 841 ccgtgttcgt gaactggatc agatccgcaa ctttctgacc ggccaggccc tgaccagcgt 901 tctggatctg ctgttcagct ttatcttctt cgcagtaatg tggtactaca gcccgaagct 961 gactctggtg attctgttct ctctgccgtg ttacgcagct tggtccgtct tcatctctcc 1021 aatcctgcgt cgccgtctgg acgacaaatt tagccgtaat gcggataatc agtccttcct 1081 ggtcgaatct gtgactgcta tcaacactat caaagccatg gcggtttctc cacagatgac 1141 taacatctgg gataagcagc tggcaggtta cgttgctgcg ggcttcaaag tgaccgttct 1201 ggcaaccatt ggtcaacagg gcattcagct gattcagaaa accgtgatga tcattaacct 1261 gtggctgggt gcgcacctgg tcatcagcgg tgacctgtcc attggccagc tgatcgcttt 1321 caacatgctg gcgggtcaaa ttgttgcccc agtaatccgt ctggcccaga tctggcagga 1381 cttccagcag gtcggtattt ctgtgacccg cctgggtgac gtcctgaact ctccgaccga 1441 aagctaccac ggtaaactgg ctctgccgga gatcaacggc gatattactt tccgtaacat 1501 ccgttttcgt tacaaaccgg attccccggt aatcctggat aacatcaatc tgtctatcaa 1561 acagggcgaa gtcatcggca tcgtgggccg tagcggcagc ggcaagtcca ctctgactaa 1621 actgattcag cgcttttaca ttccagaaaa cggtcaggtt ctgattgacg gtcacgacct 1681 ggctctggcg gacccgaact ggctgcgtcg tcaggtgggc gtagtgctgc aagataacgt 1741 gctgctgaac cgtagcatca tcgataacat cagcctggct aacccgggca tgtccgtgga 1801 aaaagttatc tatgctgcga aactggcagg cgcgcacgat ttcatctctg aactgcgtga 1861 aggctacaac accatcgtag gcgaacaggg cgctggcctg tccggcggtc aacgtcagcg 1921 tatcgctatt gcacgtgcac tggtgaacaa cccgaaaatc ctgatcttcg acgaggccac 1981 ttctgctctg gattacgaat ctgaacatat tatcatgcgt aatatgcaca agatctgcaa 2041 aggtcgcacc gtaatcatca tcgcccaccg tctgagcacc gtaaagaacg ccgaccgcat 2101 catcgtgatg gaaaaaggta agatcgttga acagggtaaa cataaagagc tgctgtccga 2161 accggaatct ctgtattctt atctgtacca gctgcaatct gattaatact agagattaaa 2221 gaggagaata ctagatgaaa acttggctga tgggcttctc tgagttcctg ctgcgttata 2281 agctggtatg gagcgaaact tggaaaatcc gtaagcaact ggataccccg gtacgtgaaa 2341 aagacgaaaa cgaatttctg ccagcacatc tggaactgat tgagacgccg gtttctcgcc 2401 gtccacgtct ggttgcttac ttcattatgg gcttcctggt tattgctgta atcctgtctg 2461 ttctgggtca ggtggaaatc gttgcgaccg cgaatggtaa actgaccctg tccggtcgtt 2521 ctaaagaaat caaaccgatc gaaaactcca tcgttaaaga gattatcgtt aaagaaggtg 2581 aatccgtacg caaaggcgat gttctgctga aactgaccgc actgggcgca gaagcggaca 2641 ctctgaaaac tcagtcctct ctgctgcaaa cccgtctgga acagacccgt taccaaatcc 2701 tgagccgttc tattgaactg aacaaactgc cggaactgaa actgccggat gaaccatact 2761 tccagaacgt aagcgaggag gaagttctgc gcctgacttc cctgatcaaa gagcagttct 2821 ctacttggca gaaccagaaa taccaaaagg aactgaacct ggacaaaaag cgtgcggagc 2881 gcctgactat cctggcccgc atcaaccgtt acgagaacct gtctcgtgtt gaaaaatctc 2941 gcctggacga cttccgtagc ctgctgcaca aacaggccat cgcaaaacac gctgtgctgg 3001 aacaggaaaa taaatacgtg gaagctgcca acgaactgcg tgtatataaa agccagctgg 3061 agcagatcga gagcgaaatc ctgtctgcga aggaggagta ccagctggtg acgcagctgt 3121 tcaaaaatga aatcctggac aaactgcgtc aaaccacgga ctctatcgaa ctgctgaccc 3181 tggaactgga gaaaaacgag gagcgccagc aggccagcgt catccgcgct ccggtatctg 3241 gtaaagttca gcaactgaaa gttcacaccg aaggtggcgt tgttaccacc gcagaaaccc 3301 tgatggttat tgtgccggaa gatgacaccc tggaagttac cgctctggtg cagaacaaag 3361 atatcggttt catcaacgtc ggtcagaacg cgatcatcaa agtagaggct ttcccgtaca 3421 ctcgttacgg ctacctggtg ggcaaggtca aaaacatcaa cctggacgct atcgaagatc 3481 agaaactggg cctggtgttc aacgtgatcg ttagcgtaga agagaacgat ctgtctaccg 3541 gtaacaaaca cattccactg tcctccggca tggcggttac tgctgaaatc aaaaccggta 3601 tgcgtagcgt gatctcctat ctgctgtctc cgctggaaga aagcgtcacc gaatctctgc 3661 acgaacgcta atactagaga ttaaagagga gaatactaga tgattgccct gaacactgcc 3721 tcgccacagg ggatgcagat gaagaagctc ctgccgatcc tgatcgggct aagtctttcc 3781 gggttcagct cactttcaca agccgagaat cttatgcaag tatatcagca ggcacggctg 3841 tcgaatccag agcttcgtaa atcagcggcc gatcgagatg ctgcgttcga gaaaattaat 3901 gaggcccggt caccgctgtt accacagctg ggtctcggcg cggattatac atacagcaac 3961 ggctaccgtg atgcgaatgg aataaatagt aacgcaacca gcgcaagctt acaactgaca 4021 cagtcgatct tcgatatgtc gaaatggcgc gcactaacgc ttcaggaaaa agcagccggg 4081 atccaagatg taacttatca gaccgaccag caaacattga ttttgaatac tgcaaccgct 4141 tactttaacg ttctgaatgc catcgacgtg ctgagctaca cccaggcaca aaaggaagcc 4201 atttatcgtc aacttgacca gactacgcag cggtttaacg ttgggttagt agcaatcacc 4261 gatgttcaaa atgcgcgtgc gcagtatgat accgttctgg ccaatgaggt caccgcacgc 4321 aacaatcttg ataatgcggt tgaacagtta cgtcagatta ccgggaacta ttatcccgag 4381 ctggcagcgc tgaatgtgga aaattttaaa actgacaaac ctcaacctgt caataccctg 4441 ctcaaggaag ccgagaaacg caacctgtct ctattgcaag ctcgcctgtc tcaagacctg 4501 gctcgggagc aaatacgcca ggcacaggac ggacatctgc ccacactgga cctgaccgca 4561 agtactggga tatccgacac cagttactct ggatcaaaaa ctcgtggcgc cgccggaaca 4621 caatacgatg actcaaatat gggccagaac aaggttggcc tgtccttcag tttaccgatc 4681 taccagggcg ggatggtgaa tagtcaagta aaacaggcgc agtataactt tgtcggtgca 4741 tcagagcaac tggaatcagc tcaccgtagc gtcgtacaga ctgtccggag tagctttaac 4801 aacattaacg cgagtatctc atcaatcaat gcttacaagc aagcagtggt ttctgcgcag 4861 tcttctttgg atgccatgga agcaggctat tcagtgggta ctcgcacgat tgttgatgtt 4921 ctcgacgcta ccacaactct ctacaacgcg aaacaggaat tagcgaatgc ccgttataac 4981 tacttaatta accaactgaa tatcaaatct gccctaggta ctctgaatga gcaagacttg 5041 cttgcgttaa ataacgccct gtccaaacct gtctcaacca atcctgagaa cgtagcacct 5101 cagacccctg aacagaacgc cattgcggac ggttacgccc ctgacagtcc tgcgcctgtg 5161 gtccagcaga cgtcagcgcg cactaccact tcaaacgggc ataatccgtt ccgcaattac 5221 tagagaaaaa aaaaccccgc ccctgacagg gcggggtttt tttt //