##gff-version 3
##sequence-region BBa_K654998 1 5264
BBa_K654998	.	engineered_region	63	5264	.	+	0	ID=BBa_M1027;Name=BBa_M1027
BBa_K654998	.	CDS	83	2206	.	+	0	ID=BBa_M1003;Name=BBa_M1003;Parent=BBa_M1027
BBa_K654998	.	CDS	83	2203	.	+	0	ID=annotation2115951;Name=HlyB;Parent=BBa_M1003
BBa_K654998	.	stop_codon	2204	2206	.	+	0	ID=annotation2115950;Name=Stop Codon;Parent=BBa_M1003
BBa_K654998	.	start_codon	83	85	.	+	0	ID=annotation2115949;Name=Start Codon;Parent=BBa_M1003
BBa_K654998	.	ribosome_entry_site	3680	3693	.	+	0	ID=BBa_B0035;Name=BBa_B0035;Parent=BBa_M1027
BBa_K654998	.	ribosome_entry_site	63	76	.	+	0	ID=BBa_B0035;Name=BBa_B0035;Parent=BBa_M1027
BBa_K654998	.	ribosome_entry_site	2215	2228	.	+	0	ID=BBa_B0035;Name=BBa_B0035;Parent=BBa_M1027
BBa_K654998	.	terminator	5226	5264	.	+	0	ID=BBa_B1006;Name=BBa_B1006;Parent=BBa_M1027
BBa_K654998	.	polyA_site	5226	5234	.	+	0	ID=annotation1898431;Name=PolyA;Parent=BBa_B1006
BBa_K654998	.	polyA_site	5257	5264	.	+	0	ID=annotation1898430;Name=PolyA;Parent=BBa_B1006
BBa_K654998	.	engineered_region	5226	5264	.	+	0	ID=annotation1898428;Name=B1006;Parent=BBa_B1006
BBa_K654998	.	stem_loop	5235	5256	.	+	0	ID=annotation1898429;Name=modified thr terminator;Parent=BBa_B1006
BBa_K654998	.	CDS	2235	3671	.	+	0	ID=BBa_M1004;Name=BBa_M1004;Parent=BBa_M1027
BBa_K654998	.	CDS	2235	3668	.	+	0	ID=annotation2115953;Name=HlyD;Parent=BBa_M1004
BBa_K654998	.	start_codon	2235	2237	.	+	0	ID=annotation2115952;Name=Start Codon;Parent=BBa_M1004
BBa_K654998	.	stop_codon	3669	3671	.	+	0	ID=annotation2115954;Name=Stop Codon;Parent=BBa_M1004
BBa_K654998	.	CDS	3700	5217	.	+	0	ID=BBa_M1028;Name=BBa_M1028;Parent=BBa_M1027
BBa_K654998	.	CDS	3700	5214	.	+	0	ID=annotation2117428;Name=TolC;Parent=BBa_M1028
BBa_K654998	.	stop_codon	5215	5217	.	+	0	ID=annotation2117427;Name=Stop;Parent=BBa_M1028
BBa_K654998	.	start_codon	3700	3702	.	+	0	ID=annotation2117426;Name=Start;Parent=BBa_M1028
BBa_K654998	.	promoter	1	54	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_K654998	.	non_covalent_binding_site	26	44	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_K654998	.	promoter	20	25	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_K654998	.	non_covalent_binding_site	1	19	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_K654998	.	engineered_region	1	54	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_K654998	.	promoter	43	48	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
>BBa_K654998
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagattaaagaggagaatac
tagatggattcctgtcacaagattgattacggcctgtatgcactggaaatcctggcacaatatcataacgtctccgtca
acccggaggaaattaaacatcgcttcgatactgacggcactggcctgggtctgaccagctggctgctggcagcgaagtc
cctggagctgaaggtaaagcaagtgaaaaagacgattgatcgtctgaacttcatcagcctgccggcactggtttggcgt
gaagatggccgtcacttcatcctgacgaaagtgtccaaagaagcgaaccgctacctgatctttgatctggagcagcgca
acccgcgcgtactggaacagagcgagttcgaggctctgtatcagggccacatcattctgattgcatctcgttcttctgt
tgcaggtaaactggcgaaattcgacttcacctggttcattccggctattatcaagtaccgtcgtattttcatcgaaacc
ctggttgtatctgttttcctgcaactgttcgctctgattactccgctgttctttcaggttgtcatggacaaagttctgg
ttcaccgtggcttttccaccctgaacgtcattactgttgcgctgagcgttgttgttgtgttcgaaatcattctgtccgg
tctgcgtacctacatcttcgcgcactctacctcccgcatcgacgttgaactgggtgcgaagctgttccgtcacctgctg
gcactgcctatcagctacttcgaatctcgtcgtgtgggcgatactgtggcccgtgttcgtgaactggatcagatccgca
actttctgaccggccaggccctgaccagcgttctggatctgctgttcagctttatcttcttcgcagtaatgtggtacta
cagcccgaagctgactctggtgattctgttctctctgccgtgttacgcagcttggtccgtcttcatctctccaatcctg
cgtcgccgtctggacgacaaatttagccgtaatgcggataatcagtccttcctggtcgaatctgtgactgctatcaaca
ctatcaaagccatggcggtttctccacagatgactaacatctgggataagcagctggcaggttacgttgctgcgggctt
caaagtgaccgttctggcaaccattggtcaacagggcattcagctgattcagaaaaccgtgatgatcattaacctgtgg
ctgggtgcgcacctggtcatcagcggtgacctgtccattggccagctgatcgctttcaacatgctggcgggtcaaattg
ttgccccagtaatccgtctggcccagatctggcaggacttccagcaggtcggtatttctgtgacccgcctgggtgacgt
cctgaactctccgaccgaaagctaccacggtaaactggctctgccggagatcaacggcgatattactttccgtaacatc
cgttttcgttacaaaccggattccccggtaatcctggataacatcaatctgtctatcaaacagggcgaagtcatcggca
tcgtgggccgtagcggcagcggcaagtccactctgactaaactgattcagcgcttttacattccagaaaacggtcaggt
tctgattgacggtcacgacctggctctggcggacccgaactggctgcgtcgtcaggtgggcgtagtgctgcaagataac
gtgctgctgaaccgtagcatcatcgataacatcagcctggctaacccgggcatgtccgtggaaaaagttatctatgctg
cgaaactggcaggcgcgcacgatttcatctctgaactgcgtgaaggctacaacaccatcgtaggcgaacagggcgctgg
cctgtccggcggtcaacgtcagcgtatcgctattgcacgtgcactggtgaacaacccgaaaatcctgatcttcgacgag
gccacttctgctctggattacgaatctgaacatattatcatgcgtaatatgcacaagatctgcaaaggtcgcaccgtaa
tcatcatcgcccaccgtctgagcaccgtaaagaacgccgaccgcatcatcgtgatggaaaaaggtaagatcgttgaaca
gggtaaacataaagagctgctgtccgaaccggaatctctgtattcttatctgtaccagctgcaatctgattaatactag
agattaaagaggagaatactagatgaaaacttggctgatgggcttctctgagttcctgctgcgttataagctggtatgg
agcgaaacttggaaaatccgtaagcaactggataccccggtacgtgaaaaagacgaaaacgaatttctgccagcacatc
tggaactgattgagacgccggtttctcgccgtccacgtctggttgcttacttcattatgggcttcctggttattgctgt
aatcctgtctgttctgggtcaggtggaaatcgttgcgaccgcgaatggtaaactgaccctgtccggtcgttctaaagaa
atcaaaccgatcgaaaactccatcgttaaagagattatcgttaaagaaggtgaatccgtacgcaaaggcgatgttctgc
tgaaactgaccgcactgggcgcagaagcggacactctgaaaactcagtcctctctgctgcaaacccgtctggaacagac
ccgttaccaaatcctgagccgttctattgaactgaacaaactgccggaactgaaactgccggatgaaccatacttccag
aacgtaagcgaggaggaagttctgcgcctgacttccctgatcaaagagcagttctctacttggcagaaccagaaatacc
aaaaggaactgaacctggacaaaaagcgtgcggagcgcctgactatcctggcccgcatcaaccgttacgagaacctgtc
tcgtgttgaaaaatctcgcctggacgacttccgtagcctgctgcacaaacaggccatcgcaaaacacgctgtgctggaa
caggaaaataaatacgtggaagctgccaacgaactgcgtgtatataaaagccagctggagcagatcgagagcgaaatcc
tgtctgcgaaggaggagtaccagctggtgacgcagctgttcaaaaatgaaatcctggacaaactgcgtcaaaccacgga
ctctatcgaactgctgaccctggaactggagaaaaacgaggagcgccagcaggccagcgtcatccgcgctccggtatct
ggtaaagttcagcaactgaaagttcacaccgaaggtggcgttgttaccaccgcagaaaccctgatggttattgtgccgg
aagatgacaccctggaagttaccgctctggtgcagaacaaagatatcggtttcatcaacgtcggtcagaacgcgatcat
caaagtagaggctttcccgtacactcgttacggctacctggtgggcaaggtcaaaaacatcaacctggacgctatcgaa
gatcagaaactgggcctggtgttcaacgtgatcgttagcgtagaagagaacgatctgtctaccggtaacaaacacattc
cactgtcctccggcatggcggttactgctgaaatcaaaaccggtatgcgtagcgtgatctcctatctgctgtctccgct
ggaagaaagcgtcaccgaatctctgcacgaacgctaatactagagattaaagaggagaatactagatgattgccctgaa
cactgcctcgccacaggggatgcagatgaagaagctcctgccgatcctgatcgggctaagtctttccgggttcagctca
ctttcacaagccgagaatcttatgcaagtatatcagcaggcacggctgtcgaatccagagcttcgtaaatcagcggccg
atcgagatgctgcgttcgagaaaattaatgaggcccggtcaccgctgttaccacagctgggtctcggcgcggattatac
atacagcaacggctaccgtgatgcgaatggaataaatagtaacgcaaccagcgcaagcttacaactgacacagtcgatc
ttcgatatgtcgaaatggcgcgcactaacgcttcaggaaaaagcagccgggatccaagatgtaacttatcagaccgacc
agcaaacattgattttgaatactgcaaccgcttactttaacgttctgaatgccatcgacgtgctgagctacacccaggc
acaaaaggaagccatttatcgtcaacttgaccagactacgcagcggtttaacgttgggttagtagcaatcaccgatgtt
caaaatgcgcgtgcgcagtatgataccgttctggccaatgaggtcaccgcacgcaacaatcttgataatgcggttgaac
agttacgtcagattaccgggaactattatcccgagctggcagcgctgaatgtggaaaattttaaaactgacaaacctca
acctgtcaataccctgctcaaggaagccgagaaacgcaacctgtctctattgcaagctcgcctgtctcaagacctggct
cgggagcaaatacgccaggcacaggacggacatctgcccacactggacctgaccgcaagtactgggatatccgacacca
gttactctggatcaaaaactcgtggcgccgccggaacacaatacgatgactcaaatatgggccagaacaaggttggcct
gtccttcagtttaccgatctaccagggcgggatggtgaatagtcaagtaaaacaggcgcagtataactttgtcggtgca
tcagagcaactggaatcagctcaccgtagcgtcgtacagactgtccggagtagctttaacaacattaacgcgagtatct
catcaatcaatgcttacaagcaagcagtggtttctgcgcagtcttctttggatgccatggaagcaggctattcagtggg
tactcgcacgattgttgatgttctcgacgctaccacaactctctacaacgcgaaacaggaattagcgaatgcccgttat
aactacttaattaaccaactgaatatcaaatctgccctaggtactctgaatgagcaagacttgcttgcgttaaataacg
ccctgtccaaacctgtctcaaccaatcctgagaacgtagcacctcagacccctgaacagaacgccattgcggacggtta
cgcccctgacagtcctgcgcctgtggtccagcagacgtcagcgcgcactaccacttcaaacgggcataatccgttccgc
aattactagagaaaaaaaaaccccgcccctgacagggcggggtttttttt