LOCUS BBa_K654999 5265 bp DNA linear 22-Nov-2024 DEFINITION Lac promoter driving Hyl BD genes and TolC ACCESSION BBa_K654999 VERSION BBa_K654999.1 FEATURES Location/Qualifiers misc_feature 3..19 /label=lac O1 promoter 20..25 /label=-35 misc_feature 26..42 /label=lac O1 promoter 43..48 /label=-10 misc_feature 1..54 /label=BBa_R0011 promoter 1..54 /label=BBa_R0011 RBS 64..77 /label=BBa_B0035 misc_feature 84..86 /label=Start Codon misc_feature 2205..2207 /label=Stop Codon CDS 84..2204 /label=HlyB CDS 84..2207 /label=BBa_M1003 RBS 2216..2229 /label=BBa_B0035 misc_feature 2236..2238 /label=Start Codon CDS 2236..3669 /label=HlyD misc_feature 3670..3672 /label=Stop Codon CDS 2236..3672 /label=BBa_M1004 RBS 3681..3694 /label=BBa_B0035 misc_feature 3701..3703 /label=Start misc_feature 5216..5218 /label=Stop CDS 3701..5215 /label=TolC CDS 3701..5218 /label=BBa_M1028 misc_feature 5227..5265 /label=B1006 stem_loop 5236..5257 /label=modified thr terminator polyA_site 5258..5265 /label=PolyA polyA_site 5227..5235 /label=PolyA terminator 5227..5265 /label=BBa_B1006 misc_feature 64..5265 /label=BBa_M1027 ORIGIN 1 aattgtgagc ggataacaat tgacattgtg agcggataac aagatactga gcacatacta 61 gagattaaag aggagaatac tagatggatt cctgtcacaa gattgattac ggcctgtatg 121 cactggaaat cctggcacaa tatcataacg tctccgtcaa cccggaggaa attaaacatc 181 gcttcgatac tgacggcact ggcctgggtc tgaccagctg gctgctggca gcgaagtccc 241 tggagctgaa ggtaaagcaa gtgaaaaaga cgattgatcg tctgaacttc atcagcctgc 301 cggcactggt ttggcgtgaa gatggccgtc acttcatcct gacgaaagtg tccaaagaag 361 cgaaccgcta cctgatcttt gatctggagc agcgcaaccc gcgcgtactg gaacagagcg 421 agttcgaggc tctgtatcag ggccacatca ttctgattgc atctcgttct tctgttgcag 481 gtaaactggc gaaattcgac ttcacctggt tcattccggc tattatcaag taccgtcgta 541 ttttcatcga aaccctggtt gtatctgttt tcctgcaact gttcgctctg attactccgc 601 tgttctttca ggttgtcatg gacaaagttc tggttcaccg tggcttttcc accctgaacg 661 tcattactgt tgcgctgagc gttgttgttg tgttcgaaat cattctgtcc ggtctgcgta 721 cctacatctt cgcgcactct acctcccgca tcgacgttga actgggtgcg aagctgttcc 781 gtcacctgct ggcactgcct atcagctact tcgaatctcg tcgtgtgggc gatactgtgg 841 cccgtgttcg tgaactggat cagatccgca actttctgac cggccaggcc ctgaccagcg 901 ttctggatct gctgttcagc tttatcttct tcgcagtaat gtggtactac agcccgaagc 961 tgactctggt gattctgttc tctctgccgt gttacgcagc ttggtccgtc ttcatctctc 1021 caatcctgcg tcgccgtctg gacgacaaat ttagccgtaa tgcggataat cagtccttcc 1081 tggtcgaatc tgtgactgct atcaacacta tcaaagccat ggcggtttct ccacagatga 1141 ctaacatctg ggataagcag ctggcaggtt acgttgctgc gggcttcaaa gtgaccgttc 1201 tggcaaccat tggtcaacag ggcattcagc tgattcagaa aaccgtgatg atcattaacc 1261 tgtggctggg tgcgcacctg gtcatcagcg gtgacctgtc cattggccag ctgatcgctt 1321 tcaacatgct ggcgggtcaa attgttgccc cagtaatccg tctggcccag atctggcagg 1381 acttccagca ggtcggtatt tctgtgaccc gcctgggtga cgtcctgaac tctccgaccg 1441 aaagctacca cggtaaactg gctctgccgg agatcaacgg cgatattact ttccgtaaca 1501 tccgttttcg ttacaaaccg gattccccgg taatcctgga taacatcaat ctgtctatca 1561 aacagggcga agtcatcggc atcgtgggcc gtagcggcag cggcaagtcc actctgacta 1621 aactgattca gcgcttttac attccagaaa acggtcaggt tctgattgac ggtcacgacc 1681 tggctctggc ggacccgaac tggctgcgtc gtcaggtggg cgtagtgctg caagataacg 1741 tgctgctgaa ccgtagcatc atcgataaca tcagcctggc taacccgggc atgtccgtgg 1801 aaaaagttat ctatgctgcg aaactggcag gcgcgcacga tttcatctct gaactgcgtg 1861 aaggctacaa caccatcgta ggcgaacagg gcgctggcct gtccggcggt caacgtcagc 1921 gtatcgctat tgcacgtgca ctggtgaaca acccgaaaat cctgatcttc gacgaggcca 1981 cttctgctct ggattacgaa tctgaacata ttatcatgcg taatatgcac aagatctgca 2041 aaggtcgcac cgtaatcatc atcgcccacc gtctgagcac cgtaaagaac gccgaccgca 2101 tcatcgtgat ggaaaaaggt aagatcgttg aacagggtaa acataaagag ctgctgtccg 2161 aaccggaatc tctgtattct tatctgtacc agctgcaatc tgattaatac tagagattaa 2221 agaggagaat actagatgaa aacttggctg atgggcttct ctgagttcct gctgcgttat 2281 aagctggtat ggagcgaaac ttggaaaatc cgtaagcaac tggatacccc ggtacgtgaa 2341 aaagacgaaa acgaatttct gccagcacat ctggaactga ttgagacgcc ggtttctcgc 2401 cgtccacgtc tggttgctta cttcattatg ggcttcctgg ttattgctgt aatcctgtct 2461 gttctgggtc aggtggaaat cgttgcgacc gcgaatggta aactgaccct gtccggtcgt 2521 tctaaagaaa tcaaaccgat cgaaaactcc atcgttaaag agattatcgt taaagaaggt 2581 gaatccgtac gcaaaggcga tgttctgctg aaactgaccg cactgggcgc agaagcggac 2641 actctgaaaa ctcagtcctc tctgctgcaa acccgtctgg aacagacccg ttaccaaatc 2701 ctgagccgtt ctattgaact gaacaaactg ccggaactga aactgccgga tgaaccatac 2761 ttccagaacg taagcgagga ggaagttctg cgcctgactt ccctgatcaa agagcagttc 2821 tctacttggc agaaccagaa ataccaaaag gaactgaacc tggacaaaaa gcgtgcggag 2881 cgcctgacta tcctggcccg catcaaccgt tacgagaacc tgtctcgtgt tgaaaaatct 2941 cgcctggacg acttccgtag cctgctgcac aaacaggcca tcgcaaaaca cgctgtgctg 3001 gaacaggaaa ataaatacgt ggaagctgcc aacgaactgc gtgtatataa aagccagctg 3061 gagcagatcg agagcgaaat cctgtctgcg aaggaggagt accagctggt gacgcagctg 3121 ttcaaaaatg aaatcctgga caaactgcgt caaaccacgg actctatcga actgctgacc 3181 ctggaactgg agaaaaacga ggagcgccag caggccagcg tcatccgcgc tccggtatct 3241 ggtaaagttc agcaactgaa agttcacacc gaaggtggcg ttgttaccac cgcagaaacc 3301 ctgatggtta ttgtgccgga agatgacacc ctggaagtta ccgctctggt gcagaacaaa 3361 gatatcggtt tcatcaacgt cggtcagaac gcgatcatca aagtagaggc tttcccgtac 3421 actcgttacg gctacctggt gggcaaggtc aaaaacatca acctggacgc tatcgaagat 3481 cagaaactgg gcctggtgtt caacgtgatc gttagcgtag aagagaacga tctgtctacc 3541 ggtaacaaac acattccact gtcctccggc atggcggtta ctgctgaaat caaaaccggt 3601 atgcgtagcg tgatctccta tctgctgtct ccgctggaag aaagcgtcac cgaatctctg 3661 cacgaacgct aatactagag attaaagagg agaatactag atgattgccc tgaacactgc 3721 ctcgccacag gggatgcaga tgaagaagct cctgccgatc ctgatcgggc taagtctttc 3781 cgggttcagc tcactttcac aagccgagaa tcttatgcaa gtatatcagc aggcacggct 3841 gtcgaatcca gagcttcgta aatcagcggc cgatcgagat gctgcgttcg agaaaattaa 3901 tgaggcccgg tcaccgctgt taccacagct gggtctcggc gcggattata catacagcaa 3961 cggctaccgt gatgcgaatg gaataaatag taacgcaacc agcgcaagct tacaactgac 4021 acagtcgatc ttcgatatgt cgaaatggcg cgcactaacg cttcaggaaa aagcagccgg 4081 gatccaagat gtaacttatc agaccgacca gcaaacattg attttgaata ctgcaaccgc 4141 ttactttaac gttctgaatg ccatcgacgt gctgagctac acccaggcac aaaaggaagc 4201 catttatcgt caacttgacc agactacgca gcggtttaac gttgggttag tagcaatcac 4261 cgatgttcaa aatgcgcgtg cgcagtatga taccgttctg gccaatgagg tcaccgcacg 4321 caacaatctt gataatgcgg ttgaacagtt acgtcagatt accgggaact attatcccga 4381 gctggcagcg ctgaatgtgg aaaattttaa aactgacaaa cctcaacctg tcaataccct 4441 gctcaaggaa gccgagaaac gcaacctgtc tctattgcaa gctcgcctgt ctcaagacct 4501 ggctcgggag caaatacgcc aggcacagga cggacatctg cccacactgg acctgaccgc 4561 aagtactggg atatccgaca ccagttactc tggatcaaaa actcgtggcg ccgccggaac 4621 acaatacgat gactcaaata tgggccagaa caaggttggc ctgtccttca gtttaccgat 4681 ctaccagggc gggatggtga atagtcaagt aaaacaggcg cagtataact ttgtcggtgc 4741 atcagagcaa ctggaatcag ctcaccgtag cgtcgtacag actgtccgga gtagctttaa 4801 caacattaac gcgagtatct catcaatcaa tgcttacaag caagcagtgg tttctgcgca 4861 gtcttctttg gatgccatgg aagcaggcta ttcagtgggt actcgcacga ttgttgatgt 4921 tctcgacgct accacaactc tctacaacgc gaaacaggaa ttagcgaatg cccgttataa 4981 ctacttaatt aaccaactga atatcaaatc tgccctaggt actctgaatg agcaagactt 5041 gcttgcgtta aataacgccc tgtccaaacc tgtctcaacc aatcctgaga acgtagcacc 5101 tcagacccct gaacagaacg ccattgcgga cggttacgcc cctgacagtc ctgcgcctgt 5161 ggtccagcag acgtcagcgc gcactaccac ttcaaacggg cataatccgt tccgcaatta 5221 ctagagaaaa aaaaaccccg cccctgacag ggcggggttt ttttt //