##gff-version 3
##sequence-region BBa_K654999 1 5265
BBa_K654999	.	engineered_region	64	5265	.	+	0	ID=BBa_M1027;Name=BBa_M1027
BBa_K654999	.	CDS	84	2207	.	+	0	ID=BBa_M1003;Name=BBa_M1003;Parent=BBa_M1027
BBa_K654999	.	CDS	84	2204	.	+	0	ID=annotation2115951;Name=HlyB;Parent=BBa_M1003
BBa_K654999	.	stop_codon	2205	2207	.	+	0	ID=annotation2115950;Name=Stop Codon;Parent=BBa_M1003
BBa_K654999	.	start_codon	84	86	.	+	0	ID=annotation2115949;Name=Start Codon;Parent=BBa_M1003
BBa_K654999	.	ribosome_entry_site	3681	3694	.	+	0	ID=BBa_B0035;Name=BBa_B0035;Parent=BBa_M1027
BBa_K654999	.	ribosome_entry_site	64	77	.	+	0	ID=BBa_B0035;Name=BBa_B0035;Parent=BBa_M1027
BBa_K654999	.	ribosome_entry_site	2216	2229	.	+	0	ID=BBa_B0035;Name=BBa_B0035;Parent=BBa_M1027
BBa_K654999	.	terminator	5227	5265	.	+	0	ID=BBa_B1006;Name=BBa_B1006;Parent=BBa_M1027
BBa_K654999	.	polyA_site	5227	5235	.	+	0	ID=annotation1898431;Name=PolyA;Parent=BBa_B1006
BBa_K654999	.	polyA_site	5258	5265	.	+	0	ID=annotation1898430;Name=PolyA;Parent=BBa_B1006
BBa_K654999	.	engineered_region	5227	5265	.	+	0	ID=annotation1898428;Name=B1006;Parent=BBa_B1006
BBa_K654999	.	stem_loop	5236	5257	.	+	0	ID=annotation1898429;Name=modified thr terminator;Parent=BBa_B1006
BBa_K654999	.	CDS	2236	3672	.	+	0	ID=BBa_M1004;Name=BBa_M1004;Parent=BBa_M1027
BBa_K654999	.	CDS	2236	3669	.	+	0	ID=annotation2115953;Name=HlyD;Parent=BBa_M1004
BBa_K654999	.	start_codon	2236	2238	.	+	0	ID=annotation2115952;Name=Start Codon;Parent=BBa_M1004
BBa_K654999	.	stop_codon	3670	3672	.	+	0	ID=annotation2115954;Name=Stop Codon;Parent=BBa_M1004
BBa_K654999	.	CDS	3701	5218	.	+	0	ID=BBa_M1028;Name=BBa_M1028;Parent=BBa_M1027
BBa_K654999	.	CDS	3701	5215	.	+	0	ID=annotation2117428;Name=TolC;Parent=BBa_M1028
BBa_K654999	.	stop_codon	5216	5218	.	+	0	ID=annotation2117427;Name=Stop;Parent=BBa_M1028
BBa_K654999	.	start_codon	3701	3703	.	+	0	ID=annotation2117426;Name=Start;Parent=BBa_M1028
BBa_K654999	.	promoter	1	54	.	+	0	ID=BBa_R0011;Name=BBa_R0011
BBa_K654999	.	operator	3	19	.	+	0	ID=annotation1999;Name=lac O1;Parent=BBa_R0011
BBa_K654999	.	promoter	20	25	.	+	0	ID=annotation2000;Name=-35;Parent=BBa_R0011
BBa_K654999	.	promoter	43	48	.	+	0	ID=annotation2002;Name=-10;Parent=BBa_R0011
BBa_K654999	.	operator	26	42	.	+	0	ID=annotation2001;Name=lac O1;Parent=BBa_R0011
BBa_K654999	.	engineered_region	1	54	.	+	0	ID=annotation7064;Name=BBa_R0011;Parent=BBa_R0011
>BBa_K654999
aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacatactagagattaaagaggagaata
ctagatggattcctgtcacaagattgattacggcctgtatgcactggaaatcctggcacaatatcataacgtctccgtc
aacccggaggaaattaaacatcgcttcgatactgacggcactggcctgggtctgaccagctggctgctggcagcgaagt
ccctggagctgaaggtaaagcaagtgaaaaagacgattgatcgtctgaacttcatcagcctgccggcactggtttggcg
tgaagatggccgtcacttcatcctgacgaaagtgtccaaagaagcgaaccgctacctgatctttgatctggagcagcgc
aacccgcgcgtactggaacagagcgagttcgaggctctgtatcagggccacatcattctgattgcatctcgttcttctg
ttgcaggtaaactggcgaaattcgacttcacctggttcattccggctattatcaagtaccgtcgtattttcatcgaaac
cctggttgtatctgttttcctgcaactgttcgctctgattactccgctgttctttcaggttgtcatggacaaagttctg
gttcaccgtggcttttccaccctgaacgtcattactgttgcgctgagcgttgttgttgtgttcgaaatcattctgtccg
gtctgcgtacctacatcttcgcgcactctacctcccgcatcgacgttgaactgggtgcgaagctgttccgtcacctgct
ggcactgcctatcagctacttcgaatctcgtcgtgtgggcgatactgtggcccgtgttcgtgaactggatcagatccgc
aactttctgaccggccaggccctgaccagcgttctggatctgctgttcagctttatcttcttcgcagtaatgtggtact
acagcccgaagctgactctggtgattctgttctctctgccgtgttacgcagcttggtccgtcttcatctctccaatcct
gcgtcgccgtctggacgacaaatttagccgtaatgcggataatcagtccttcctggtcgaatctgtgactgctatcaac
actatcaaagccatggcggtttctccacagatgactaacatctgggataagcagctggcaggttacgttgctgcgggct
tcaaagtgaccgttctggcaaccattggtcaacagggcattcagctgattcagaaaaccgtgatgatcattaacctgtg
gctgggtgcgcacctggtcatcagcggtgacctgtccattggccagctgatcgctttcaacatgctggcgggtcaaatt
gttgccccagtaatccgtctggcccagatctggcaggacttccagcaggtcggtatttctgtgacccgcctgggtgacg
tcctgaactctccgaccgaaagctaccacggtaaactggctctgccggagatcaacggcgatattactttccgtaacat
ccgttttcgttacaaaccggattccccggtaatcctggataacatcaatctgtctatcaaacagggcgaagtcatcggc
atcgtgggccgtagcggcagcggcaagtccactctgactaaactgattcagcgcttttacattccagaaaacggtcagg
ttctgattgacggtcacgacctggctctggcggacccgaactggctgcgtcgtcaggtgggcgtagtgctgcaagataa
cgtgctgctgaaccgtagcatcatcgataacatcagcctggctaacccgggcatgtccgtggaaaaagttatctatgct
gcgaaactggcaggcgcgcacgatttcatctctgaactgcgtgaaggctacaacaccatcgtaggcgaacagggcgctg
gcctgtccggcggtcaacgtcagcgtatcgctattgcacgtgcactggtgaacaacccgaaaatcctgatcttcgacga
ggccacttctgctctggattacgaatctgaacatattatcatgcgtaatatgcacaagatctgcaaaggtcgcaccgta
atcatcatcgcccaccgtctgagcaccgtaaagaacgccgaccgcatcatcgtgatggaaaaaggtaagatcgttgaac
agggtaaacataaagagctgctgtccgaaccggaatctctgtattcttatctgtaccagctgcaatctgattaatacta
gagattaaagaggagaatactagatgaaaacttggctgatgggcttctctgagttcctgctgcgttataagctggtatg
gagcgaaacttggaaaatccgtaagcaactggataccccggtacgtgaaaaagacgaaaacgaatttctgccagcacat
ctggaactgattgagacgccggtttctcgccgtccacgtctggttgcttacttcattatgggcttcctggttattgctg
taatcctgtctgttctgggtcaggtggaaatcgttgcgaccgcgaatggtaaactgaccctgtccggtcgttctaaaga
aatcaaaccgatcgaaaactccatcgttaaagagattatcgttaaagaaggtgaatccgtacgcaaaggcgatgttctg
ctgaaactgaccgcactgggcgcagaagcggacactctgaaaactcagtcctctctgctgcaaacccgtctggaacaga
cccgttaccaaatcctgagccgttctattgaactgaacaaactgccggaactgaaactgccggatgaaccatacttcca
gaacgtaagcgaggaggaagttctgcgcctgacttccctgatcaaagagcagttctctacttggcagaaccagaaatac
caaaaggaactgaacctggacaaaaagcgtgcggagcgcctgactatcctggcccgcatcaaccgttacgagaacctgt
ctcgtgttgaaaaatctcgcctggacgacttccgtagcctgctgcacaaacaggccatcgcaaaacacgctgtgctgga
acaggaaaataaatacgtggaagctgccaacgaactgcgtgtatataaaagccagctggagcagatcgagagcgaaatc
ctgtctgcgaaggaggagtaccagctggtgacgcagctgttcaaaaatgaaatcctggacaaactgcgtcaaaccacgg
actctatcgaactgctgaccctggaactggagaaaaacgaggagcgccagcaggccagcgtcatccgcgctccggtatc
tggtaaagttcagcaactgaaagttcacaccgaaggtggcgttgttaccaccgcagaaaccctgatggttattgtgccg
gaagatgacaccctggaagttaccgctctggtgcagaacaaagatatcggtttcatcaacgtcggtcagaacgcgatca
tcaaagtagaggctttcccgtacactcgttacggctacctggtgggcaaggtcaaaaacatcaacctggacgctatcga
agatcagaaactgggcctggtgttcaacgtgatcgttagcgtagaagagaacgatctgtctaccggtaacaaacacatt
ccactgtcctccggcatggcggttactgctgaaatcaaaaccggtatgcgtagcgtgatctcctatctgctgtctccgc
tggaagaaagcgtcaccgaatctctgcacgaacgctaatactagagattaaagaggagaatactagatgattgccctga
acactgcctcgccacaggggatgcagatgaagaagctcctgccgatcctgatcgggctaagtctttccgggttcagctc
actttcacaagccgagaatcttatgcaagtatatcagcaggcacggctgtcgaatccagagcttcgtaaatcagcggcc
gatcgagatgctgcgttcgagaaaattaatgaggcccggtcaccgctgttaccacagctgggtctcggcgcggattata
catacagcaacggctaccgtgatgcgaatggaataaatagtaacgcaaccagcgcaagcttacaactgacacagtcgat
cttcgatatgtcgaaatggcgcgcactaacgcttcaggaaaaagcagccgggatccaagatgtaacttatcagaccgac
cagcaaacattgattttgaatactgcaaccgcttactttaacgttctgaatgccatcgacgtgctgagctacacccagg
cacaaaaggaagccatttatcgtcaacttgaccagactacgcagcggtttaacgttgggttagtagcaatcaccgatgt
tcaaaatgcgcgtgcgcagtatgataccgttctggccaatgaggtcaccgcacgcaacaatcttgataatgcggttgaa
cagttacgtcagattaccgggaactattatcccgagctggcagcgctgaatgtggaaaattttaaaactgacaaacctc
aacctgtcaataccctgctcaaggaagccgagaaacgcaacctgtctctattgcaagctcgcctgtctcaagacctggc
tcgggagcaaatacgccaggcacaggacggacatctgcccacactggacctgaccgcaagtactgggatatccgacacc
agttactctggatcaaaaactcgtggcgccgccggaacacaatacgatgactcaaatatgggccagaacaaggttggcc
tgtccttcagtttaccgatctaccagggcgggatggtgaatagtcaagtaaaacaggcgcagtataactttgtcggtgc
atcagagcaactggaatcagctcaccgtagcgtcgtacagactgtccggagtagctttaacaacattaacgcgagtatc
tcatcaatcaatgcttacaagcaagcagtggtttctgcgcagtcttctttggatgccatggaagcaggctattcagtgg
gtactcgcacgattgttgatgttctcgacgctaccacaactctctacaacgcgaaacaggaattagcgaatgcccgtta
taactacttaattaaccaactgaatatcaaatctgccctaggtactctgaatgagcaagacttgcttgcgttaaataac
gccctgtccaaacctgtctcaaccaatcctgagaacgtagcacctcagacccctgaacagaacgccattgcggacggtt
acgcccctgacagtcctgcgcctgtggtccagcagacgtcagcgcgcactaccacttcaaacgggcataatccgttccg
caattactagagaaaaaaaaaccccgcccctgacagggcggggtttttttt