BBa_K654999
type: ComponentDefinition
persistent identity: BBa K654999
display id: BBa_K654999
version: 1
was generated by: iGEM to SBOL conversion
description: Lac promoter driving Hyl BD genes and TolC
created: 2011-07-20T11:00:00Z
modified: 2015-05-08T01:13:02Z
mutable provenance: composite
owned by: james
owned by: myers
mutable description: sample
discontinued: false
dominant: false
group u list: _831_
m user id: 0
owner id: 2425
owning group id: 303
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: composite
star: false
creator: Charles Miller
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_M1027
component: BBa_R0011
sequence annotation: BBa_R0011
sequence annotation: BBa_M1027
sequence: https://synbiohub.org/public/igem/BBa_K654999_sequence/1

BBa_M1027
type: Component
persistent identity: component2123899
display id: component2123899
version: 1
top level: BBa_K654999
definition: BBa_M1027
access: public

BBa_R0011
type: Component
persistent identity: component2123874
display id: component2123874
version: 1
top level: BBa_K654999
definition: lacI+pL
access: public

BBa_R0011
type: SequenceAnnotation
persistent identity: annotation2123874
display id: annotation2123874
version: 1
top level: BBa_K654999
location: https://synbiohub.org/public/igem/BBa_K654999/annotation2123874/range2123874/1
component: BBa_R0011

https://synbiohub.org/public/igem/BBa_K654999/annotation2123874/range2123874/1
type: Range
persistent identity: range2123874
display id: range2123874
version: 1
top level: BBa_K654999
direction: direction/forward
start: 1
end: 54
orientation: inline

BBa_M1027
type: SequenceAnnotation
persistent identity: annotation2123899
display id: annotation2123899
version: 1
top level: BBa_K654999
location: https://synbiohub.org/public/igem/BBa_K654999/annotation2123899/range2123899/1
component: BBa_M1027

https://synbiohub.org/public/igem/BBa_K654999/annotation2123899/range2123899/1
type: Range
persistent identity: range2123899
display id: range2123899
version: 1
top level: BBa_K654999
direction: direction/reverse
start: 64
end: 5265
orientation: inline

lacI+pL
type: ComponentDefinition
persistent identity: BBa R0011
display id: BBa_R0011
version: 1
was generated by: iGEM to SBOL conversion
description: Promoter (lacI regulated, lambda pL hybrid)
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: represillator of Elowitz and Leibler (2000)
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) The PLlac 0-1 promoter is a hybrid regulatory region consisting of the promoter P(L) of phage lambda with the cI binding sites replaced with lacO1. The hybrid design allows for strong promotion that can nevertheless be tightly repressed by LacI, the Lac inhibitor (i.e. repressor) (<bb_part>BBa_C0010</bb_part>) ([LUTZ97]). The activity of the promoter can be regulated over a >600-fold range by IPTG in E.Coli DH5-alpha-Z1 (same paper reference).
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: hybrid promoter design to create strong promoter that is, at the same time, highly repressible. note that the upstream operator installed in this hybrid is slightly different than the one in the original source (Lutz and Bujard, 1997). the most upstream operator region is slightly truncated in the represillator version, so that both operators in the hybrid are the same sequence. see references for details. also, the sequence has been truncated after the transcriptional start site. LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and increase transcription. This part is incompatible with environments containing lactose or lactose analogs.
star: true
creator: Neelaksh Varshney, Grace Kenney, Daniel Shen, Samantha Sutton
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: BBa_R0011
sequence annotation: lac O1
sequence annotation: -35
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_R0011_sequence/1

BBa_R0011
type: SequenceAnnotation
persistent identity: annotation7064
display id: annotation7064
version: 1
top level: lacI+pL
location: https://synbiohub.org/public/igem/BBa_R0011/annotation7064/range7064/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_R0011/annotation7064/range7064/1
type: Range
persistent identity: range7064
display id: range7064
version: 1
top level: lacI+pL
direction: direction/forward
start: 1
end: 54
orientation: inline

lac O1
type: SequenceAnnotation
persistent identity: annotation1999
display id: annotation1999
version: 1
top level: lacI+pL
location: https://synbiohub.org/public/igem/BBa_R0011/annotation1999/range1999/1
role: SO:0000057
role: feature/operator

https://synbiohub.org/public/igem/BBa_R0011/annotation1999/range1999/1
type: Range
persistent identity: range1999
display id: range1999
version: 1
top level: lacI+pL
direction: direction/forward
start: 3
end: 19
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2000
display id: annotation2000
version: 1
top level: lacI+pL
location: https://synbiohub.org/public/igem/BBa_R0011/annotation2000/range2000/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0011/annotation2000/range2000/1
type: Range
persistent identity: range2000
display id: range2000
version: 1
top level: lacI+pL
direction: direction/forward
start: 20
end: 25
orientation: inline

lac O1
type: SequenceAnnotation
persistent identity: annotation2001
display id: annotation2001
version: 1
top level: lacI+pL
location: https://synbiohub.org/public/igem/BBa_R0011/annotation2001/range2001/1
role: SO:0000057
role: feature/operator

https://synbiohub.org/public/igem/BBa_R0011/annotation2001/range2001/1
type: Range
persistent identity: range2001
display id: range2001
version: 1
top level: lacI+pL
direction: direction/forward
start: 26
end: 42
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2002
display id: annotation2002
version: 1
top level: lacI+pL
location: https://synbiohub.org/public/igem/BBa_R0011/annotation2002/range2002/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0011/annotation2002/range2002/1
type: Range
persistent identity: range2002
display id: range2002
version: 1
top level: lacI+pL
direction: direction/forward
start: 43
end: 48
orientation: inline

BBa_M1027
type: ComponentDefinition
persistent identity: BBa M1027
display id: BBa_M1027
version: 1
was generated by: iGEM to SBOL conversion
description: RBS HlyB RBS HlyD RBS TolC Term
created: 2011-04-19T11:00:00Z
modified: 2015-05-08T01:13:52Z
mutable provenance: See Subparts
owned by: james
owned by: myers
mutable description: Codes for the HlyB and HlyD membrane proteins necessary for Type I secretion.
discontinued: false
dominant: false
experience: experience/None
group u list: _768_
m user id: 0
owner id: 8961
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: None
star: false
creator: Alex Hatch
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_B1006
component: BBa_M1028
component: BBa_B0035
component: BBa_M1004
component: BBa_M1003
sequence annotation: BBa_B0035
sequence annotation: BBa_M1003
sequence annotation: BBa_M1004
sequence annotation: BBa_M1028
sequence annotation: BBa_B1006
sequence: https://synbiohub.org/public/igem/BBa_M1027_sequence/1

BBa_B1006
type: Component
persistent identity: component2117590
display id: component2117590
version: 1
top level: BBa_M1027
definition: BBa_B1006
access: public

BBa_M1028
type: Component
persistent identity: component2117585
display id: component2117585
version: 1
top level: BBa_M1027
definition: BBa_M1028
access: public

BBa_B0035
type: Component
persistent identity: component2117581
display id: component2117581
version: 1
top level: BBa_M1027
definition: BBa_B0035
access: public

BBa_M1004
type: Component
persistent identity: component2117580
display id: component2117580
version: 1
top level: BBa_M1027
definition: BBa_M1004
access: public

BBa_B0035
type: Component
persistent identity: component2117576
display id: component2117576
version: 1
top level: BBa_M1027
definition: BBa_B0035
access: public

BBa_M1003
type: Component
persistent identity: component2117575
display id: component2117575
version: 1
top level: BBa_M1027
definition: BBa_M1003
access: public

BBa_B0035
type: Component
persistent identity: component2117571
display id: component2117571
version: 1
top level: BBa_M1027
definition: BBa_B0035
access: public

BBa_B0035
type: SequenceAnnotation
persistent identity: annotation2117571
display id: annotation2117571
version: 1
top level: BBa_M1027
location: https://synbiohub.org/public/igem/BBa_M1027/annotation2117571/range2117571/1
component: BBa_B0035

https://synbiohub.org/public/igem/BBa_M1027/annotation2117571/range2117571/1
type: Range
persistent identity: range2117571
display id: range2117571
version: 1
top level: BBa_M1027
direction: direction/reverse
start: 1
end: 14
orientation: inline

BBa_M1003
type: SequenceAnnotation
persistent identity: annotation2117575
display id: annotation2117575
version: 1
top level: BBa_M1027
location: https://synbiohub.org/public/igem/BBa_M1027/annotation2117575/range2117575/1
component: BBa_M1003

https://synbiohub.org/public/igem/BBa_M1027/annotation2117575/range2117575/1
type: Range
persistent identity: range2117575
display id: range2117575
version: 1
top level: BBa_M1027
direction: direction/reverse
start: 21
end: 2144
orientation: inline

BBa_B0035
type: SequenceAnnotation
persistent identity: annotation2117576
display id: annotation2117576
version: 1
top level: BBa_M1027
location: https://synbiohub.org/public/igem/BBa_M1027/annotation2117576/range2117576/1
component: BBa_B0035

https://synbiohub.org/public/igem/BBa_M1027/annotation2117576/range2117576/1
type: Range
persistent identity: range2117576
display id: range2117576
version: 1
top level: BBa_M1027
direction: direction/reverse
start: 2153
end: 2166
orientation: inline

BBa_M1004
type: SequenceAnnotation
persistent identity: annotation2117580
display id: annotation2117580
version: 1
top level: BBa_M1027
location: https://synbiohub.org/public/igem/BBa_M1027/annotation2117580/range2117580/1
component: BBa_M1004

https://synbiohub.org/public/igem/BBa_M1027/annotation2117580/range2117580/1
type: Range
persistent identity: range2117580
display id: range2117580
version: 1
top level: BBa_M1027
direction: direction/reverse
start: 2173
end: 3609
orientation: inline

BBa_B0035
type: SequenceAnnotation
persistent identity: annotation2117581
display id: annotation2117581
version: 1
top level: BBa_M1027
location: https://synbiohub.org/public/igem/BBa_M1027/annotation2117581/range2117581/1
component: BBa_B0035

https://synbiohub.org/public/igem/BBa_M1027/annotation2117581/range2117581/1
type: Range
persistent identity: range2117581
display id: range2117581
version: 1
top level: BBa_M1027
direction: direction/reverse
start: 3618
end: 3631
orientation: inline

BBa_M1028
type: SequenceAnnotation
persistent identity: annotation2117585
display id: annotation2117585
version: 1
top level: BBa_M1027
location: https://synbiohub.org/public/igem/BBa_M1027/annotation2117585/range2117585/1
component: BBa_M1028

https://synbiohub.org/public/igem/BBa_M1027/annotation2117585/range2117585/1
type: Range
persistent identity: range2117585
display id: range2117585
version: 1
top level: BBa_M1027
direction: direction/reverse
start: 3638
end: 5155
orientation: inline

BBa_B1006
type: SequenceAnnotation
persistent identity: annotation2117590
display id: annotation2117590
version: 1
top level: BBa_M1027
location: https://synbiohub.org/public/igem/BBa_M1027/annotation2117590/range2117590/1
component: BBa_B1006

https://synbiohub.org/public/igem/BBa_M1027/annotation2117590/range2117590/1
type: Range
persistent identity: range2117590
display id: range2117590
version: 1
top level: BBa_M1027
direction: direction/reverse
start: 5164
end: 5202
orientation: inline

BBa_B0035
type: ComponentDefinition
persistent identity: BBa B0035
display id: BBa_B0035
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (B0030 derivative)
created: 2004-01-27T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: -- No description --
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Jason Kelly
type: DnaRegion
role: partType/RBS
role: SO:0000139
sequence: https://synbiohub.org/public/igem/BBa_B0035_sequence/1

BBa_B1006
type: ComponentDefinition
persistent identity: BBa B1006
display id: BBa_B1006
version: 1
was generated by: iGEM to SBOL conversion
description: Terminator (artificial, large, %T~>90)
created: 2006-08-30T11:00:00Z
modified: 2015-08-31T04:07:21Z
mutable provenance: modified E. coli thr terminator, replaced all A-T pairs in stem with C-G pairs
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Artificial terminator, estimated %T~>90% *8bp stem, 6nt loop *Bidirectional, estimated reverse %T~>90%
discontinued: false
dominant: true
experience: experience/Works
group u list: _41_
m user id: 0
owner id: 745
owning group id: 41
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Bidirectional, with the reverse estimated to be less effective than the forward. Has a polyA tail of 9 residues.
star: true
creator: Haiyao Huang
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: B1006
sequence annotation: PolyA
sequence annotation: modified thr terminator
sequence: https://synbiohub.org/public/igem/BBa_B1006_sequence/1

B1006
type: SequenceAnnotation
persistent identity: annotation1898428
display id: annotation1898428
version: 1
top level: BBa_B1006
location: https://synbiohub.org/public/igem/BBa_B1006/annotation1898428/range1898428/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B1006/annotation1898428/range1898428/1
type: Range
persistent identity: range1898428
display id: range1898428
version: 1
top level: BBa_B1006
direction: direction/forward
start: 1
end: 39
orientation: inline

PolyA
type: SequenceAnnotation
persistent identity: annotation1898431
display id: annotation1898431
version: 1
top level: BBa_B1006
location: https://synbiohub.org/public/igem/BBa_B1006/annotation1898431/range1898431/1
role: SO:0000553
role: feature/polya

https://synbiohub.org/public/igem/BBa_B1006/annotation1898431/range1898431/1
type: Range
persistent identity: range1898431
display id: range1898431
version: 1
top level: BBa_B1006
direction: direction/reverse
start: 1
end: 9
orientation: inline

modified thr terminator
type: SequenceAnnotation
persistent identity: annotation1898429
display id: annotation1898429
version: 1
top level: BBa_B1006
location: https://synbiohub.org/public/igem/BBa_B1006/annotation1898429/range1898429/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B1006/annotation1898429/range1898429/1
type: Range
persistent identity: range1898429
display id: range1898429
version: 1
top level: BBa_B1006
direction: direction/forward
start: 10
end: 31
orientation: inline

PolyA
type: SequenceAnnotation
persistent identity: annotation1898430
display id: annotation1898430
version: 1
top level: BBa_B1006
location: https://synbiohub.org/public/igem/BBa_B1006/annotation1898430/range1898430/1
role: SO:0000553
role: feature/polya

https://synbiohub.org/public/igem/BBa_B1006/annotation1898430/range1898430/1
type: Range
persistent identity: range1898430
display id: range1898430
version: 1
top level: BBa_B1006
direction: direction/forward
start: 32
end: 39
orientation: inline

BBa_M1003
type: ComponentDefinition
persistent identity: BBa M1003
display id: BBa_M1003
version: 1
was generated by: iGEM to SBOL conversion
description: HlyB membrane protein
created: 2011-04-12T11:00:00Z
modified: 2015-05-08T01:13:51Z
mutable provenance: Genomic. Escherichia coli UT189
owned by: myers
owned by: james
mutable description: Membrane protein involved in type I secretion.
discontinued: false
dominant: false
group u list: _768_
m user id: 0
owner id: 8961
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: Codon optimized for expression in E. coli while avoiding the BioBricks standard restriction sites.
star: false
creator: Alex Hatch
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: Start Codon
sequence annotation: HlyB
sequence annotation: Stop Codon
sequence: https://synbiohub.org/public/igem/BBa_M1003_sequence/1

Start Codon
type: SequenceAnnotation
persistent identity: annotation2115949
display id: annotation2115949
version: 1
top level: BBa_M1003
location: https://synbiohub.org/public/igem/BBa_M1003/annotation2115949/range2115949/1
role: SO:0000318
role: feature/start

https://synbiohub.org/public/igem/BBa_M1003/annotation2115949/range2115949/1
type: Range
persistent identity: range2115949
display id: range2115949
version: 1
top level: BBa_M1003
direction: direction/forward
start: 1
end: 3
orientation: inline

HlyB
type: SequenceAnnotation
persistent identity: annotation2115951
display id: annotation2115951
version: 1
top level: BBa_M1003
location: https://synbiohub.org/public/igem/BBa_M1003/annotation2115951/range2115951/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_M1003/annotation2115951/range2115951/1
type: Range
persistent identity: range2115951
display id: range2115951
version: 1
top level: BBa_M1003
direction: direction/forward
start: 1
end: 2121
orientation: inline

Stop Codon
type: SequenceAnnotation
persistent identity: annotation2115950
display id: annotation2115950
version: 1
top level: BBa_M1003
location: https://synbiohub.org/public/igem/BBa_M1003/annotation2115950/range2115950/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_M1003/annotation2115950/range2115950/1
type: Range
persistent identity: range2115950
display id: range2115950
version: 1
top level: BBa_M1003
direction: direction/forward
start: 2122
end: 2124
orientation: inline

BBa_M1004
type: ComponentDefinition
persistent identity: BBa M1004
display id: BBa_M1004
version: 1
was generated by: iGEM to SBOL conversion
description: HlyD Membrane Protein
created: 2011-04-12T11:00:00Z
modified: 2015-05-08T01:13:51Z
mutable provenance: Genomic. Escherichia coli UTI89
owned by: james
owned by: myers
mutable description: Membrane protein involved in type I secretion. When protein is fused to the HlyA signal peptide, this protein aids in protein secretion.
discontinued: false
dominant: false
group u list: _768_
m user id: 0
owner id: 8961
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: Codon optimized for expression in E. coli while avoiding BioBricks standard restriction sites.
star: false
creator: Alex Hatch
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: Start Codon
sequence annotation: HlyD
sequence annotation: Stop Codon
sequence: https://synbiohub.org/public/igem/BBa_M1004_sequence/1

Start Codon
type: SequenceAnnotation
persistent identity: annotation2115952
display id: annotation2115952
version: 1
top level: BBa_M1004
location: https://synbiohub.org/public/igem/BBa_M1004/annotation2115952/range2115952/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_M1004/annotation2115952/range2115952/1
type: Range
persistent identity: range2115952
display id: range2115952
version: 1
top level: BBa_M1004
direction: direction/forward
start: 1
end: 3
orientation: inline

HlyD
type: SequenceAnnotation
persistent identity: annotation2115953
display id: annotation2115953
version: 1
top level: BBa_M1004
location: https://synbiohub.org/public/igem/BBa_M1004/annotation2115953/range2115953/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_M1004/annotation2115953/range2115953/1
type: Range
persistent identity: range2115953
display id: range2115953
version: 1
top level: BBa_M1004
direction: direction/forward
start: 1
end: 1434
orientation: inline

Stop Codon
type: SequenceAnnotation
persistent identity: annotation2115954
display id: annotation2115954
version: 1
top level: BBa_M1004
location: https://synbiohub.org/public/igem/BBa_M1004/annotation2115954/range2115954/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_M1004/annotation2115954/range2115954/1
type: Range
persistent identity: range2115954
display id: range2115954
version: 1
top level: BBa_M1004
direction: direction/forward
start: 1435
end: 1437
orientation: inline

BBa_M1028
type: ComponentDefinition
persistent identity: BBa M1028
display id: BBa_M1028
version: 1
was generated by: iGEM to SBOL conversion
description: tolC-Outer membrane protein
created: 2011-04-25T11:00:00Z
modified: 2015-05-08T01:13:52Z
mutable provenance: E. coli genome, UTI89
owned by: james
owned by: myers
mutable description: TolC is an outer membrane protein that is essential in the type I secretion pathway together with membrane proteins HlyB and HlyD.
discontinued: false
dominant: false
group u list: _768_
m user id: 0
owner id: 8961
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: Sequence was modified to remove incompatible PstI sites as well as to avoid the presence of any of the Zn finger binding sequences for the Zif268-HIV, Zif23-GCN4, and Blues Zn fingers.
star: false
creator: Alex Hatch
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Start
sequence annotation: TolC
sequence annotation: Stop
sequence: https://synbiohub.org/public/igem/BBa_M1028_sequence/1

Start
type: SequenceAnnotation
persistent identity: annotation2117426
display id: annotation2117426
version: 1
top level: BBa_M1028
location: https://synbiohub.org/public/igem/BBa_M1028/annotation2117426/range2117426/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_M1028/annotation2117426/range2117426/1
type: Range
persistent identity: range2117426
display id: range2117426
version: 1
top level: BBa_M1028
direction: direction/forward
start: 1
end: 3
orientation: inline

TolC
type: SequenceAnnotation
persistent identity: annotation2117428
display id: annotation2117428
version: 1
top level: BBa_M1028
location: https://synbiohub.org/public/igem/BBa_M1028/annotation2117428/range2117428/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_M1028/annotation2117428/range2117428/1
type: Range
persistent identity: range2117428
display id: range2117428
version: 1
top level: BBa_M1028
direction: direction/forward
start: 1
end: 1515
orientation: inline

Stop
type: SequenceAnnotation
persistent identity: annotation2117427
display id: annotation2117427
version: 1
top level: BBa_M1028
location: https://synbiohub.org/public/igem/BBa_M1028/annotation2117427/range2117427/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_M1028/annotation2117427/range2117427/1
type: Range
persistent identity: range2117427
display id: range2117427
version: 1
top level: BBa_M1028
direction: direction/forward
start: 1516
end: 1518
orientation: inline

https://synbiohub.org/public/igem/BBa_K654999_sequence/1
type: Sequence
persistent identity: BBa K654999 sequence
display id: BBa_K654999_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacatactagagattaaagaggagaatactagatggattcctgtcacaagattgattacggcctgtatgcactggaaatcctggcacaatatcataacgtctccgtcaacccggaggaaattaaacatcgcttcgatactgacggcactggcctgggtctgaccagctggctgctggcagcgaagtccctggagctgaaggtaaagcaagtgaaaaagacgattgatcgtctgaacttcatcagcctgccggcactggtttggcgtgaagatggccgtcacttcatcctgacgaaagtgtccaaagaagcgaaccgctacctgatctttgatctggagcagcgcaacccgcgcgtactggaacagagcgagttcgaggctctgtatcagggccacatcattctgattgcatctcgttcttctgttgcaggtaaactggcgaaattcgacttcacctggttcattccggctattatcaagtaccgtcgtattttcatcgaaaccctggttgtatctgttttcctgcaactgttcgctctgattactccgctgttctttcaggttgtcatggacaaagttctggttcaccgtggcttttccaccctgaacgtcattactgttgcgctgagcgttgttgttgtgttcgaaatcattctgtccggtctgcgtacctacatcttcgcgcactctacctcccgcatcgacgttgaactgggtgcgaagctgttccgtcacctgctggcactgcctatcagctacttcgaatctcgtcgtgtgggcgatactgtggcccgtgttcgtgaactggatcagatccgcaactttctgaccggccaggccctgaccagcgttctggatctgctgttcagctttatcttcttcgcagtaatgtggtactacagcccgaagctgactctggtgattctgttctctctgccgtgttacgcagcttggtccgtcttcatctctccaatcctgcgtcgccgtctggacgacaaatttagccgtaatgcggataatcagtccttcctggtcgaatctgtgactgctatcaacactatcaaagccatggcggtttctccacagatgactaacatctgggataagcagctggcaggttacgttgctgcgggcttcaaagtgaccgttctggcaaccattggtcaacagggcattcagctgattcagaaaaccgtgatgatcattaacctgtggctgggtgcgcacctggtcatcagcggtgacctgtccattggccagctgatcgctttcaacatgctggcgggtcaaattgttgccccagtaatccgtctggcccagatctggcaggacttccagcaggtcggtatttctgtgacccgcctgggtgacgtcctgaactctccgaccgaaagctaccacggtaaactggctctgccggagatcaacggcgatattactttccgtaacatccgttttcgttacaaaccggattccccggtaatcctggataacatcaatctgtctatcaaacagggcgaagtcatcggcatcgtgggccgtagcggcagcggcaagtccactctgactaaactgattcagcgcttttacattccagaaaacggtcaggttctgattgacggtcacgacctggctctggcggacccgaactggctgcgtcgtcaggtgggcgtagtgctgcaagataacgtgctgctgaaccgtagcatcatcgataacatcagcctggctaacccgggcatgtccgtggaaaaagttatctatgctgcgaaactggcaggcgcgcacgatttcatctctgaactgcgtgaaggctacaacaccatcgtaggcgaacagggcgctggcctgtccggcggtcaacgtcagcgtatcgctattgcacgtgcactggtgaacaacccgaaaatcctgatcttcgacgaggccacttctgctctggattacgaa…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0011_sequence/1
type: Sequence
persistent identity: BBa R0011 sequence
display id: BBa_R0011_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcaca
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M1027_sequence/1
type: Sequence
persistent identity: BBa M1027 sequence
display id: BBa_M1027_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: attaaagaggagaatactagatggattcctgtcacaagattgattacggcctgtatgcactggaaatcctggcacaatatcataacgtctccgtcaacccggaggaaattaaacatcgcttcgatactgacggcactggcctgggtctgaccagctggctgctggcagcgaagtccctggagctgaaggtaaagcaagtgaaaaagacgattgatcgtctgaacttcatcagcctgccggcactggtttggcgtgaagatggccgtcacttcatcctgacgaaagtgtccaaagaagcgaaccgctacctgatctttgatctggagcagcgcaacccgcgcgtactggaacagagcgagttcgaggctctgtatcagggccacatcattctgattgcatctcgttcttctgttgcaggtaaactggcgaaattcgacttcacctggttcattccggctattatcaagtaccgtcgtattttcatcgaaaccctggttgtatctgttttcctgcaactgttcgctctgattactccgctgttctttcaggttgtcatggacaaagttctggttcaccgtggcttttccaccctgaacgtcattactgttgcgctgagcgttgttgttgtgttcgaaatcattctgtccggtctgcgtacctacatcttcgcgcactctacctcccgcatcgacgttgaactgggtgcgaagctgttccgtcacctgctggcactgcctatcagctacttcgaatctcgtcgtgtgggcgatactgtggcccgtgttcgtgaactggatcagatccgcaactttctgaccggccaggccctgaccagcgttctggatctgctgttcagctttatcttcttcgcagtaatgtggtactacagcccgaagctgactctggtgattctgttctctctgccgtgttacgcagcttggtccgtcttcatctctccaatcctgcgtcgccgtctggacgacaaatttagccgtaatgcggataatcagtccttcctggtcgaatctgtgactgctatcaacactatcaaagccatggcggtttctccacagatgactaacatctgggataagcagctggcaggttacgttgctgcgggcttcaaagtgaccgttctggcaaccattggtcaacagggcattcagctgattcagaaaaccgtgatgatcattaacctgtggctgggtgcgcacctggtcatcagcggtgacctgtccattggccagctgatcgctttcaacatgctggcgggtcaaattgttgccccagtaatccgtctggcccagatctggcaggacttccagcaggtcggtatttctgtgacccgcctgggtgacgtcctgaactctccgaccgaaagctaccacggtaaactggctctgccggagatcaacggcgatattactttccgtaacatccgttttcgttacaaaccggattccccggtaatcctggataacatcaatctgtctatcaaacagggcgaagtcatcggcatcgtgggccgtagcggcagcggcaagtccactctgactaaactgattcagcgcttttacattccagaaaacggtcaggttctgattgacggtcacgacctggctctggcggacccgaactggctgcgtcgtcaggtgggcgtagtgctgcaagataacgtgctgctgaaccgtagcatcatcgataacatcagcctggctaacccgggcatgtccgtggaaaaagttatctatgctgcgaaactggcaggcgcgcacgatttcatctctgaactgcgtgaaggctacaacaccatcgtaggcgaacagggcgctggcctgtccggcggtcaacgtcagcgtatcgctattgcacgtgcactggtgaacaacccgaaaatcctgatcttcgacgaggccacttctgctctggattacgaatctgaacatattatcatgcgtaatatgcacaagatctgcaaaggtcgcaccgtaatcatcatc…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0035_sequence/1
type: Sequence
persistent identity: BBa B0035 sequence
display id: BBa_B0035_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: attaaagaggagaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B1006_sequence/1
type: Sequence
persistent identity: BBa B1006 sequence
display id: BBa_B1006_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaaaaaaaaccccgcccctgacagggcggggtttttttt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M1003_sequence/1
type: Sequence
persistent identity: BBa M1003 sequence
display id: BBa_M1003_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggattcctgtcacaagattgattacggcctgtatgcactggaaatcctggcacaatatcataacgtctccgtcaacccggaggaaattaaacatcgcttcgatactgacggcactggcctgggtctgaccagctggctgctggcagcgaagtccctggagctgaaggtaaagcaagtgaaaaagacgattgatcgtctgaacttcatcagcctgccggcactggtttggcgtgaagatggccgtcacttcatcctgacgaaagtgtccaaagaagcgaaccgctacctgatctttgatctggagcagcgcaacccgcgcgtactggaacagagcgagttcgaggctctgtatcagggccacatcattctgattgcatctcgttcttctgttgcaggtaaactggcgaaattcgacttcacctggttcattccggctattatcaagtaccgtcgtattttcatcgaaaccctggttgtatctgttttcctgcaactgttcgctctgattactccgctgttctttcaggttgtcatggacaaagttctggttcaccgtggcttttccaccctgaacgtcattactgttgcgctgagcgttgttgttgtgttcgaaatcattctgtccggtctgcgtacctacatcttcgcgcactctacctcccgcatcgacgttgaactgggtgcgaagctgttccgtcacctgctggcactgcctatcagctacttcgaatctcgtcgtgtgggcgatactgtggcccgtgttcgtgaactggatcagatccgcaactttctgaccggccaggccctgaccagcgttctggatctgctgttcagctttatcttcttcgcagtaatgtggtactacagcccgaagctgactctggtgattctgttctctctgccgtgttacgcagcttggtccgtcttcatctctccaatcctgcgtcgccgtctggacgacaaatttagccgtaatgcggataatcagtccttcctggtcgaatctgtgactgctatcaacactatcaaagccatggcggtttctccacagatgactaacatctgggataagcagctggcaggttacgttgctgcgggcttcaaagtgaccgttctggcaaccattggtcaacagggcattcagctgattcagaaaaccgtgatgatcattaacctgtggctgggtgcgcacctggtcatcagcggtgacctgtccattggccagctgatcgctttcaacatgctggcgggtcaaattgttgccccagtaatccgtctggcccagatctggcaggacttccagcaggtcggtatttctgtgacccgcctgggtgacgtcctgaactctccgaccgaaagctaccacggtaaactggctctgccggagatcaacggcgatattactttccgtaacatccgttttcgttacaaaccggattccccggtaatcctggataacatcaatctgtctatcaaacagggcgaagtcatcggcatcgtgggccgtagcggcagcggcaagtccactctgactaaactgattcagcgcttttacattccagaaaacggtcaggttctgattgacggtcacgacctggctctggcggacccgaactggctgcgtcgtcaggtgggcgtagtgctgcaagataacgtgctgctgaaccgtagcatcatcgataacatcagcctggctaacccgggcatgtccgtggaaaaagttatctatgctgcgaaactggcaggcgcgcacgatttcatctctgaactgcgtgaaggctacaacaccatcgtaggcgaacagggcgctggcctgtccggcggtcaacgtcagcgtatcgctattgcacgtgcactggtgaacaacccgaaaatcctgatcttcgacgaggccacttctgctctggattacgaatctgaacatattatcatgcgtaatatgcacaagatctgcaaaggtcgcaccgtaatcatcatcgcccaccgtctgagcaccgt…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M1004_sequence/1
type: Sequence
persistent identity: BBa M1004 sequence
display id: BBa_M1004_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaaaacttggctgatgggcttctctgagttcctgctgcgttataagctggtatggagcgaaacttggaaaatccgtaagcaactggataccccggtacgtgaaaaagacgaaaacgaatttctgccagcacatctggaactgattgagacgccggtttctcgccgtccacgtctggttgcttacttcattatgggcttcctggttattgctgtaatcctgtctgttctgggtcaggtggaaatcgttgcgaccgcgaatggtaaactgaccctgtccggtcgttctaaagaaatcaaaccgatcgaaaactccatcgttaaagagattatcgttaaagaaggtgaatccgtacgcaaaggcgatgttctgctgaaactgaccgcactgggcgcagaagcggacactctgaaaactcagtcctctctgctgcaaacccgtctggaacagacccgttaccaaatcctgagccgttctattgaactgaacaaactgccggaactgaaactgccggatgaaccatacttccagaacgtaagcgaggaggaagttctgcgcctgacttccctgatcaaagagcagttctctacttggcagaaccagaaataccaaaaggaactgaacctggacaaaaagcgtgcggagcgcctgactatcctggcccgcatcaaccgttacgagaacctgtctcgtgttgaaaaatctcgcctggacgacttccgtagcctgctgcacaaacaggccatcgcaaaacacgctgtgctggaacaggaaaataaatacgtggaagctgccaacgaactgcgtgtatataaaagccagctggagcagatcgagagcgaaatcctgtctgcgaaggaggagtaccagctggtgacgcagctgttcaaaaatgaaatcctggacaaactgcgtcaaaccacggactctatcgaactgctgaccctggaactggagaaaaacgaggagcgccagcaggccagcgtcatccgcgctccggtatctggtaaagttcagcaactgaaagttcacaccgaaggtggcgttgttaccaccgcagaaaccctgatggttattgtgccggaagatgacaccctggaagttaccgctctggtgcagaacaaagatatcggtttcatcaacgtcggtcagaacgcgatcatcaaagtagaggctttcccgtacactcgttacggctacctggtgggcaaggtcaaaaacatcaacctggacgctatcgaagatcagaaactgggcctggtgttcaacgtgatcgttagcgtagaagagaacgatctgtctaccggtaacaaacacattccactgtcctccggcatggcggttactgctgaaatcaaaaccggtatgcgtagcgtgatctcctatctgctgtctccgctggaagaaagcgtcaccgaatctctgcacgaacgctaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M1028_sequence/1
type: Sequence
persistent identity: BBa M1028 sequence
display id: BBa_M1028_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgattgccctgaacactgcctcgccacaggggatgcagatgaagaagctcctgccgatcctgatcgggctaagtctttccgggttcagctcactttcacaagccgagaatcttatgcaagtatatcagcaggcacggctgtcgaatccagagcttcgtaaatcagcggccgatcgagatgctgcgttcgagaaaattaatgaggcccggtcaccgctgttaccacagctgggtctcggcgcggattatacatacagcaacggctaccgtgatgcgaatggaataaatagtaacgcaaccagcgcaagcttacaactgacacagtcgatcttcgatatgtcgaaatggcgcgcactaacgcttcaggaaaaagcagccgggatccaagatgtaacttatcagaccgaccagcaaacattgattttgaatactgcaaccgcttactttaacgttctgaatgccatcgacgtgctgagctacacccaggcacaaaaggaagccatttatcgtcaacttgaccagactacgcagcggtttaacgttgggttagtagcaatcaccgatgttcaaaatgcgcgtgcgcagtatgataccgttctggccaatgaggtcaccgcacgcaacaatcttgataatgcggttgaacagttacgtcagattaccgggaactattatcccgagctggcagcgctgaatgtggaaaattttaaaactgacaaacctcaacctgtcaataccctgctcaaggaagccgagaaacgcaacctgtctctattgcaagctcgcctgtctcaagacctggctcgggagcaaatacgccaggcacaggacggacatctgcccacactggacctgaccgcaagtactgggatatccgacaccagttactctggatcaaaaactcgtggcgccgccggaacacaatacgatgactcaaatatgggccagaacaaggttggcctgtccttcagtttaccgatctaccagggcgggatggtgaatagtcaagtaaaacaggcgcagtataactttgtcggtgcatcagagcaactggaatcagctcaccgtagcgtcgtacagactgtccggagtagctttaacaacattaacgcgagtatctcatcaatcaatgcttacaagcaagcagtggtttctgcgcagtcttctttggatgccatggaagcaggctattcagtgggtactcgcacgattgttgatgttctcgacgctaccacaactctctacaacgcgaaacaggaattagcgaatgcccgttataactacttaattaaccaactgaatatcaaatctgccctaggtactctgaatgagcaagacttgcttgcgttaaataacgccctgtccaaacctgtctcaaccaatcctgagaacgtagcacctcagacccctgaacagaacgccattgcggacggttacgcccctgacagtcctgcgcctgtggtccagcagacgtcagcgcgcactaccacttcaaacgggcataatccgttccgcaat
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z