Lac promoter driving Hyl BD genes and TolC
Promoter (lacI regulated, lambda pL hybrid)
represillator of Elowitz and Leibler (2000)
Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) The PLlac 0-1 promoter is a hybrid regulatory region consisting of the promoter P(L) of phage lambda with the cI binding sites replaced with lacO1. The hybrid design allows for strong promotion that can nevertheless be tightly repressed by LacI, the Lac inhibitor (i.e. repressor) (<bb_part>BBa_C0010</bb_part>) ([LUTZ97]). The activity of the promoter can be regulated over a >600-fold range by IPTG in E.Coli DH5-alpha-Z1 (same paper reference).
hybrid promoter design to create strong promoter that is, at the same time, highly repressible. note that the upstream operator installed in this hybrid is slightly different than the one in the original source (Lutz and Bujard, 1997). the most upstream operator region is slightly truncated in the represillator version, so that both operators in the hybrid are the same sequence. see references for details. also, the sequence has been truncated after the transcriptional start site. LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and increase transcription. This part is incompatible with environments containing lactose or lactose analogs.
Neelaksh Varshney, Grace Kenney, Daniel Shen, Samantha Sutton
Codes for the HlyB and HlyD membrane proteins necessary for Type I secretion.
modified E. coli thr terminator, replaced all A-T pairs in stem with C-G pairs
Artificial terminator, estimated %T~>90% *8bp stem, 6nt loop *Bidirectional, estimated reverse %T~>90%
Bidirectional, with the reverse estimated to be less effective than the forward. Has a polyA tail of 9 residues.
Membrane protein involved in type I secretion.
Codon optimized for expression in E. coli while avoiding the BioBricks standard restriction sites.
Membrane protein involved in type I secretion. When protein is fused to the HlyA signal peptide, this protein aids in protein secretion.
Codon optimized for expression in E. coli while avoiding BioBricks standard restriction sites.
TolC is an outer membrane protein that is essential in the type I secretion pathway together with membrane proteins HlyB and HlyD.
Sequence was modified to remove incompatible PstI sites as well as to avoid the presence of any of the Zn finger binding sequences for the Zif268-HIV, Zif23-GCN4, and Blues Zn fingers.
Conversion of the iGEM parts registry to SBOL2.1