IPTG Reporter Using Cohesin-Gly-Ser-Gly-Cohesin
http://partsregistry.org/Part:BBa_K656002, http://partsregistry.org/Part:BBa_K656004, http://partsregistry.org/Part:BBa_K243004, http://partsregistry.org/Part:BBa_K091110, http://partsregistry.org/Part:BBa_J61100, http://partsregistry.org/Part:BBa_B0015
Two different cohesin molecules linked by a Gly-Ser-Gly linker form a protein that will be expressed in the IPTG.
Double terminator consisting of BBa_B0010 and BBa_B0012
Promoter (lacI regulated, lambda pL hybrid)
represillator of Elowitz and Leibler (2000)
Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) The PLlac 0-1 promoter is a hybrid regulatory region consisting of the promoter P(L) of phage lambda with the cI binding sites replaced with lacO1. The hybrid design allows for strong promotion that can nevertheless be tightly repressed by LacI, the Lac inhibitor (i.e. repressor) (<bb_part>BBa_C0010</bb_part>) ([LUTZ97]). The activity of the promoter can be regulated over a >600-fold range by IPTG in E.Coli DH5-alpha-Z1 (same paper reference).
hybrid promoter design to create strong promoter that is, at the same time, highly repressible. note that the upstream operator installed in this hybrid is slightly different than the one in the original source (Lutz and Bujard, 1997). the most upstream operator region is slightly truncated in the represillator version, so that both operators in the hybrid are the same sequence. see references for details. also, the sequence has been truncated after the transcriptional start site. LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and increase transcription. This part is incompatible with environments containing lactose or lactose analogs.
Neelaksh Varshney, Grace Kenney, Daniel Shen, Samantha Sutton
Oligos synthesized by sigma. Hybridized by PCR.
This linker was used to connect two different parts. The sequence produced the aminoacids Gly-Gly-Ser-Gly.
Cohesin region of Scaffolding Protein A from Clostridium thermocellum
Cohesin region from Scaffolding Protein A from Clostridium thermocellum. This binds to any of the dockerin enzymes from Clostridium thermocellum.
In the assembly of the Clostridium cellulolyticum cellulosome, the multiple cohesin modules of the scaffolding protein CipC serve as receptors for cellulolytic enzymes which bear a dockerin module. This is a Type I cellulolyticum cohesin module (Cc-cohesin)
Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
Transcription terminator for the E.coli RNA polymerase.
Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
Transcriptional terminator consisting of a 64 bp stem-loop.
Conversion of the iGEM parts registry to SBOL2.1