LOCUS       BBa_K656012             2532 bp    DNA     linear       16-Jun-2024
DEFINITION  PowerCell
ACCESSION   BBa_K656012
VERSION     BBa_K656012.1  
FEATURES             Location/Qualifiers
     promoter        1..359
                     /label=BBa_K656010
     misc_feature    372..375
                     /label=conserved
     RBS             368..379
                     /label=BBa_B0034
     misc_feature    388..393
                     /label=spacer
     misc_feature    1642..1649
                     /label=spacer
     misc_feature    702..702
                     /label=mutation results in pstI cut site
     CDS             388..1649
                     /label=BBa_K656011
     misc_feature    1662..1665
                     /label=conserved
     RBS             1658..1669
                     /label=BBa_B0034
     CDS             1676..2395
                     /label=GFP protein
     CDS             1676..2395
                     /label=BBa_E0040
     stem_loop       2415..2458
                     /label=stem_loop
     misc_feature    2404..2483
                     /label=BBa_B0010
     terminator      2404..2483
                     /label=BBa_B0010
     stem_loop       2499..2518
                     /label=T7 TE
     misc_feature    2525..2525
                     /label=stop
     polyA_site      2519..2532
                     /label=polya
     misc_feature    2492..2532
                     /label=BBa_B0012
     terminator      2492..2532
                     /label=BBa_B0012
     misc_feature    1658..2532
                     /label=BBa_I13504
ORIGIN
        1 tcatcaataa atatttaaaa ttttgttatg aagttgtttt cttgggcaag ccatagcaat
       61 catcaaataa ctaccataga caactaactc aaatcgcttt tttgagtctg acttatgctt
      121 catatatctt tacaacaagt attgagcata gtttagtgaa tgaggtaaac acgctcctgt
      181 catgtagctg atttataagg aaagagagaa attccgcaaa tcgtagtttt taaaagtgtc
      241 tattacattg tttaaagtta tgtagccttt tacttacggt ggataagcga ctgcggtaaa
      301 ctatgccttg attatgattc tttcacagaa aagaacactg gaaaggagcc gttttttcat
      361 actagagaaa gaggagaaat actagagtac tagatggcac tgaatattcc attcagaaat
      421 gcgtactatc gttttgcatc cagttactca tttctctttt ttatttcctg gtcgctgtgg
      481 tggtcgttat acgctatttg gctgaaagga catctagggt tgacagggac ggaattaggt
      541 acactttatt cggtcaacca gtttaccagc attctattta tgatgttcta cggcatcgtt
      601 caggataaac tcggtctgaa gaaaccgctc atctggtgta tgagtttcat cctggtcttg
      661 accggaccgt ttatgattta cgtttatgaa ccgttactgc agagcaattt ttctgtaggt
      721 ctaattctgg gggcgctatt ttttggcttg gggtatctgg cgggatgcgg tttgcttgat
      781 agcttcaccg aaaaaatggc gcgaaatttt catttcgaat atggaacagc gcgcgcctgg
      841 ggatcttttg gctatgctat tggcgcgttc tttgccggca tattttttag tatcagtccc
      901 catatcaact tctggttggt ctcgctattt ggcgctgtat ttatgatgat caacatgcgt
      961 tttaaagata aggatcacca gtgcgtagcg gcagatgcgg gaggggtaaa aaaagaggat
     1021 tttatcgcag ttttcaagga tcgaaacttc tgggttttcg tcatatttat tgtggggacg
     1081 tggtctttct ataacatttt tgatcaacaa ctttttcctg tcttttattc aggtttattc
     1141 gaatcacacg atgtaggaac gcgcctgtat ggttatctca actcattcca ggtggtactc
     1201 gaagcgctgt gcatggcgat tattcctttc tttgtgaatc gggtagggcc aaaaaatgca
     1261 ttacttatcg gagttgtgat tatggcgttg cgtatccttt cctgcgcgct gttcgttaac
     1321 ccctggatta tttcattagt gaagttgtta catgccattg aggttccact ttgtgtcata
     1381 tccgtcttca aatacagcgt ggcaaacttt gataagcgcc tgtcgtcgac gatctttctg
     1441 attggttttc aaattgccag ttcgcttggg attgtgctgc tttcaacgcc gactgggata
     1501 ctctttgacc acgcaggcta ccagacagtt ttcttcgcaa tttcgggtat tgtctgcctg
     1561 atgttgctat ttggcatttt cttcttgagt aaaaaacgcg agcaaatagt tatggaaacg
     1621 cctgtacctt cagcaatata gtactagagt actagagaaa gaggagaaat actagatgcg
     1681 taaaggagaa gaacttttca ctggagttgt cccaattctt gttgaattag atggtgatgt
     1741 taatgggcac aaattttctg tcagtggaga gggtgaaggt gatgcaacat acggaaaact
     1801 tacccttaaa tttatttgca ctactggaaa actacctgtt ccatggccaa cacttgtcac
     1861 tactttcggt tatggtgttc aatgctttgc gagataccca gatcatatga aacagcatga
     1921 ctttttcaag agtgccatgc ccgaaggtta tgtacaggaa agaactatat ttttcaaaga
     1981 tgacgggaac tacaagacac gtgctgaagt caagtttgaa ggtgataccc ttgttaatag
     2041 aatcgagtta aaaggtattg attttaaaga agatggaaac attcttggac acaaattgga
     2101 atacaactat aactcacaca atgtatacat catggcagac aaacaaaaga atggaatcaa
     2161 agttaacttc aaaattagac acaacattga agatggaagc gttcaactag cagaccatta
     2221 tcaacaaaat actccaattg gcgatggccc tgtcctttta ccagacaacc attacctgtc
     2281 cacacaatct gccctttcga aagatcccaa cgaaaagaga gaccacatgg tccttcttga
     2341 gtttgtaaca gctgctggga ttacacatgg catggatgaa ctatacaaat aataatacta
     2401 gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct
     2461 gttgtttgtc ggtgaacgct ctctactaga gtcacactgg ctcaccttcg ggtgggcctt
     2521 tctgcgttta ta
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