iccdB1.0
type: ComponentDefinition
persistent identity: BBa K658001
display id: BBa_K658001
version: 1
was generated by: iGEM to SBOL conversion
description: a bacteria population-control device with RBS1.0 driven by lacl+pL
created: 2011-09-19T11:00:00Z
modified: 2015-07-08T03:16:04Z
mutable provenance: K176003
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: The device is designed to build a programmed bacterial death circuit, which is based on the quorum sensing system of Vibrio fischeri. The LuxI protein synthesizes a small, diffusible acyl-homoserinelactone (AHL) signaling molecule. The AHL accumulates as the cell density increases. At sufficiently high concentrations, it binds the LuxR, which induces the expression of the killer gene ccdB under the control of a promoter lux pR.Sufficiently high levels of CcdB which is a bacterial toxin that targets DNA gyrase cause cell death. Low cell density doesn???t have the ability to produce sufficient LuxR/AHL complex to activate the promoter lux pR. The programmed death circuit ends and the cell density increases. When the cells reach a certain concentration, the death circuit is restarted. Back and forth, the programmed death is achieved in the dynamic process of growth and death. In that way, the bacteria population is programmed to maintain one certain cell density.
discontinued: true
dominant: false
experience: experience/Works
group u list: _835_
m user id: 4206
owner id: 10207
owning group id: 9
sample status: In stock
status: status/Deleted
bookmark: false
mutable notes: The bacteria population-control system was once a two-plasmid system. In order to enhance its stability and efficiency, we integrated target sequences into one plasmid.
star: false
creator: Yidan Hu
type: DnaRegion
role: SO:0000804
role: partType/Device
component: BBa_R0011
component: BBa_B0015
component: BBa_K176003
component: BBa_B0034
component: BBa_K658000
component: BBa_F1610
sequence annotation: BBa_R0011
sequence annotation: BBa_F1610
sequence annotation: BBa_K658000
sequence annotation: BBa_B0034
sequence annotation: BBa_K176003
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_K658001_sequence/1

BBa_R0011
type: Component
persistent identity: component2311670
display id: component2311670
version: 1
top level: iccdB1.0
definition: lacI+pL
access: public

BBa_B0015
type: Component
persistent identity: component2311735
display id: component2311735
version: 1
top level: iccdB1.0
definition: BBa_B0015
access: public

BBa_K176003
type: Component
persistent identity: component2311728
display id: component2311728
version: 1
top level: iccdB1.0
definition: lacZa-ccdB
access: public

BBa_B0034
type: Component
persistent identity: component2311714
display id: component2311714
version: 1
top level: iccdB1.0
definition: BBa_B0034
access: public

BBa_K658000
type: Component
persistent identity: component2311712
display id: component2311712
version: 1
top level: iccdB1.0
definition: BBa_K658000
access: public

BBa_F1610
type: Component
persistent identity: component2311687
display id: component2311687
version: 1
top level: iccdB1.0
definition: BBa_F1610
access: public

BBa_R0011
type: SequenceAnnotation
persistent identity: annotation2311670
display id: annotation2311670
version: 1
top level: iccdB1.0
location: https://synbiohub.org/public/igem/BBa_K658001/annotation2311670/range2311670/1
component: BBa_R0011

https://synbiohub.org/public/igem/BBa_K658001/annotation2311670/range2311670/1
type: Range
persistent identity: range2311670
display id: range2311670
version: 1
top level: iccdB1.0
direction: direction/forward
start: 1
end: 54
orientation: inline

BBa_F1610
type: SequenceAnnotation
persistent identity: annotation2311687
display id: annotation2311687
version: 1
top level: iccdB1.0
location: https://synbiohub.org/public/igem/BBa_K658001/annotation2311687/range2311687/1
component: BBa_F1610

https://synbiohub.org/public/igem/BBa_K658001/annotation2311687/range2311687/1
type: Range
persistent identity: range2311687
display id: range2311687
version: 1
top level: iccdB1.0
direction: direction/reverse
start: 64
end: 861
orientation: inline

BBa_K658000
type: SequenceAnnotation
persistent identity: annotation2311712
display id: annotation2311712
version: 1
top level: iccdB1.0
location: https://synbiohub.org/public/igem/BBa_K658001/annotation2311712/range2311712/1
component: BBa_K658000

https://synbiohub.org/public/igem/BBa_K658001/annotation2311712/range2311712/1
type: Range
persistent identity: range2311712
display id: range2311712
version: 1
top level: iccdB1.0
direction: direction/reverse
start: 870
end: 1931
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2311714
display id: annotation2311714
version: 1
top level: iccdB1.0
location: https://synbiohub.org/public/igem/BBa_K658001/annotation2311714/range2311714/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K658001/annotation2311714/range2311714/1
type: Range
persistent identity: range2311714
display id: range2311714
version: 1
top level: iccdB1.0
direction: direction/reverse
start: 1940
end: 1951
orientation: inline

BBa_K176003
type: SequenceAnnotation
persistent identity: annotation2311728
display id: annotation2311728
version: 1
top level: iccdB1.0
location: https://synbiohub.org/public/igem/BBa_K658001/annotation2311728/range2311728/1
component: BBa_K176003

https://synbiohub.org/public/igem/BBa_K658001/annotation2311728/range2311728/1
type: Range
persistent identity: range2311728
display id: range2311728
version: 1
top level: iccdB1.0
direction: direction/reverse
start: 1958
end: 2437
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2311735
display id: annotation2311735
version: 1
top level: iccdB1.0
location: https://synbiohub.org/public/igem/BBa_K658001/annotation2311735/range2311735/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K658001/annotation2311735/range2311735/1
type: Range
persistent identity: range2311735
display id: range2311735
version: 1
top level: iccdB1.0
direction: direction/reverse
start: 2446
end: 2574
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

BBa_B0015
type: ComponentDefinition
persistent identity: BBa B0015
display id: BBa_B0015
version: 1
was generated by: iGEM to SBOL conversion
description: double terminator (B0010-B0012)
created: 2003-07-16T11:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Double terminator consisting of BBa_B0010 and BBa_B0012
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Reshma Shetty
type: DnaRegion
role: partType/Terminator
role: SO:0000141
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_B0015_sequence/1

BBa_B0012
type: Component
persistent identity: component1916612
display id: component1916612
version: 1
top level: BBa_B0015
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1916610
display id: component1916610
version: 1
top level: BBa_B0015
definition: BBa_B0010
access: public

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1916610
display id: annotation1916610
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
type: Range
persistent identity: range1916610
display id: range1916610
version: 1
top level: BBa_B0015
direction: direction/forward
start: 1
end: 80
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1916612
display id: annotation1916612
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
type: Range
persistent identity: range1916612
display id: range1916612
version: 1
top level: BBa_B0015
direction: direction/forward
start: 89
end: 129
orientation: inline

lacI+pL
type: ComponentDefinition
persistent identity: BBa R0011
display id: BBa_R0011
version: 1
was generated by: iGEM to SBOL conversion
description: Promoter (lacI regulated, lambda pL hybrid)
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: represillator of Elowitz and Leibler (2000)
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) The PLlac 0-1 promoter is a hybrid regulatory region consisting of the promoter P(L) of phage lambda with the cI binding sites replaced with lacO1. The hybrid design allows for strong promotion that can nevertheless be tightly repressed by LacI, the Lac inhibitor (i.e. repressor) (<bb_part>BBa_C0010</bb_part>) ([LUTZ97]). The activity of the promoter can be regulated over a >600-fold range by IPTG in E.Coli DH5-alpha-Z1 (same paper reference).
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: hybrid promoter design to create strong promoter that is, at the same time, highly repressible. note that the upstream operator installed in this hybrid is slightly different than the one in the original source (Lutz and Bujard, 1997). the most upstream operator region is slightly truncated in the represillator version, so that both operators in the hybrid are the same sequence. see references for details. also, the sequence has been truncated after the transcriptional start site. LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and increase transcription. This part is incompatible with environments containing lactose or lactose analogs.
star: true
creator: Neelaksh Varshney, Grace Kenney, Daniel Shen, Samantha Sutton
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: BBa_R0011
sequence annotation: lac O1
sequence annotation: -35
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_R0011_sequence/1

BBa_R0011
type: SequenceAnnotation
persistent identity: annotation7064
display id: annotation7064
version: 1
top level: lacI+pL
location: https://synbiohub.org/public/igem/BBa_R0011/annotation7064/range7064/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_R0011/annotation7064/range7064/1
type: Range
persistent identity: range7064
display id: range7064
version: 1
top level: lacI+pL
direction: direction/forward
start: 1
end: 54
orientation: inline

lac O1
type: SequenceAnnotation
persistent identity: annotation1999
display id: annotation1999
version: 1
top level: lacI+pL
location: https://synbiohub.org/public/igem/BBa_R0011/annotation1999/range1999/1
role: SO:0000057
role: feature/operator

https://synbiohub.org/public/igem/BBa_R0011/annotation1999/range1999/1
type: Range
persistent identity: range1999
display id: range1999
version: 1
top level: lacI+pL
direction: direction/forward
start: 3
end: 19
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2000
display id: annotation2000
version: 1
top level: lacI+pL
location: https://synbiohub.org/public/igem/BBa_R0011/annotation2000/range2000/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0011/annotation2000/range2000/1
type: Range
persistent identity: range2000
display id: range2000
version: 1
top level: lacI+pL
direction: direction/forward
start: 20
end: 25
orientation: inline

lac O1
type: SequenceAnnotation
persistent identity: annotation2001
display id: annotation2001
version: 1
top level: lacI+pL
location: https://synbiohub.org/public/igem/BBa_R0011/annotation2001/range2001/1
role: SO:0000057
role: feature/operator

https://synbiohub.org/public/igem/BBa_R0011/annotation2001/range2001/1
type: Range
persistent identity: range2001
display id: range2001
version: 1
top level: lacI+pL
direction: direction/forward
start: 26
end: 42
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2002
display id: annotation2002
version: 1
top level: lacI+pL
location: https://synbiohub.org/public/igem/BBa_R0011/annotation2002/range2002/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0011/annotation2002/range2002/1
type: Range
persistent identity: range2002
display id: range2002
version: 1
top level: lacI+pL
direction: direction/forward
start: 43
end: 48
orientation: inline

BBa_F1610
type: ComponentDefinition
persistent identity: BBa F1610
display id: BBa_F1610
version: 1
was generated by: iGEM to SBOL conversion
description: 3OC6HSL Sender Device
created: 2003-12-04T12:00:00Z
modified: 2015-08-31T04:07:27Z
mutable provenance: Formerly I0461
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: This device accepts PoPS as input and produces the LuxI enzyme. This enzyme produces 3OC6HSL.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Caitlin Conboy and Jennifer Braff
type: DnaRegion
role: partType/Signalling
role: SO:0000804
component: BBa_B0034
component: BBa_B0010
component: BBa_C0061
component: BBa_B0012
sequence annotation: BBa_B0034
sequence annotation: BBa_C0061
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_F1610_sequence/1

BBa_B0034
type: Component
persistent identity: component943133
display id: component943133
version: 1
top level: BBa_F1610
definition: BBa_B0034
access: public

BBa_B0010
type: Component
persistent identity: component943150
display id: component943150
version: 1
top level: BBa_F1610
definition: BBa_B0010
access: public

BBa_C0061
type: Component
persistent identity: component943143
display id: component943143
version: 1
top level: BBa_F1610
definition: luxI
access: public

BBa_B0012
type: Component
persistent identity: component943160
display id: component943160
version: 1
top level: BBa_F1610
definition: BBa_B0012
access: public

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation943133
display id: annotation943133
version: 1
top level: BBa_F1610
location: https://synbiohub.org/public/igem/BBa_F1610/annotation943133/range943133/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_F1610/annotation943133/range943133/1
type: Range
persistent identity: range943133
display id: range943133
version: 1
top level: BBa_F1610
direction: direction/forward
start: 1
end: 12
orientation: inline

BBa_C0061
type: SequenceAnnotation
persistent identity: annotation943143
display id: annotation943143
version: 1
top level: BBa_F1610
location: https://synbiohub.org/public/igem/BBa_F1610/annotation943143/range943143/1
component: BBa_C0061

https://synbiohub.org/public/igem/BBa_F1610/annotation943143/range943143/1
type: Range
persistent identity: range943143
display id: range943143
version: 1
top level: BBa_F1610
direction: direction/forward
start: 19
end: 636
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation943150
display id: annotation943150
version: 1
top level: BBa_F1610
location: https://synbiohub.org/public/igem/BBa_F1610/annotation943150/range943150/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_F1610/annotation943150/range943150/1
type: Range
persistent identity: range943150
display id: range943150
version: 1
top level: BBa_F1610
direction: direction/forward
start: 670
end: 749
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation943160
display id: annotation943160
version: 1
top level: BBa_F1610
location: https://synbiohub.org/public/igem/BBa_F1610/annotation943160/range943160/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_F1610/annotation943160/range943160/1
type: Range
persistent identity: range943160
display id: range943160
version: 1
top level: BBa_F1610
direction: direction/forward
start: 758
end: 798
orientation: inline

BBa_K658000
type: ComponentDefinition
persistent identity: BBa K658000
display id: BBa_K658000
version: 1
was generated by: iGEM to SBOL conversion
description: 3OC6HSL Receiver Device controlled by PLlac 0-1
created: 2011-09-03T11:00:00Z
modified: 2015-05-08T01:13:02Z
mutable provenance: Part: BBa_F2621 Part: BBa_R0011
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: This device senses the 3OC6HSL concentration in the media and produces output PoPS according to the transfer function given below.LuxR production is controlled by Placo-1 (Part:BBA_R0011).
discontinued: false
dominant: false
experience: experience/Works
group u list: _835_
m user id: 0
owner id: 10207
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: This device can be activated by IPTG so that the expression of downstream sequence can be controlled by adding inducer IPTG.
star: false
creator: Xin Wu
type: DnaRegion
role: SO:0000804
role: partType/Device
component: BBa_R0062
component: BBa_R0011
component: BBa_B0012
component: BBa_B0010
component: BBa_C0062
component: BBa_B0034
sequence annotation: BBa_R0011
sequence annotation: BBa_B0034
sequence annotation: BBa_C0062
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_R0062
sequence: https://synbiohub.org/public/igem/BBa_K658000_sequence/1

BBa_R0062
type: Component
persistent identity: component2264031
display id: component2264031
version: 1
top level: BBa_K658000
definition: lux pR
access: public

BBa_R0011
type: Component
persistent identity: component2264011
display id: component2264011
version: 1
top level: BBa_K658000
definition: lacI+pL
access: public

BBa_B0012
type: Component
persistent identity: component2264026
display id: component2264026
version: 1
top level: BBa_K658000
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component2264024
display id: component2264024
version: 1
top level: BBa_K658000
definition: BBa_B0010
access: public

BBa_C0062
type: Component
persistent identity: component2264020
display id: component2264020
version: 1
top level: BBa_K658000
definition: luxr
access: public

BBa_B0034
type: Component
persistent identity: component2264017
display id: component2264017
version: 1
top level: BBa_K658000
definition: BBa_B0034
access: public

BBa_R0011
type: SequenceAnnotation
persistent identity: annotation2264011
display id: annotation2264011
version: 1
top level: BBa_K658000
location: https://synbiohub.org/public/igem/BBa_K658000/annotation2264011/range2264011/1
component: BBa_R0011

https://synbiohub.org/public/igem/BBa_K658000/annotation2264011/range2264011/1
type: Range
persistent identity: range2264011
display id: range2264011
version: 1
top level: BBa_K658000
direction: direction/forward
start: 1
end: 54
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2264017
display id: annotation2264017
version: 1
top level: BBa_K658000
location: https://synbiohub.org/public/igem/BBa_K658000/annotation2264017/range2264017/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K658000/annotation2264017/range2264017/1
type: Range
persistent identity: range2264017
display id: range2264017
version: 1
top level: BBa_K658000
direction: direction/reverse
start: 64
end: 75
orientation: inline

BBa_C0062
type: SequenceAnnotation
persistent identity: annotation2264020
display id: annotation2264020
version: 1
top level: BBa_K658000
location: https://synbiohub.org/public/igem/BBa_K658000/annotation2264020/range2264020/1
component: BBa_C0062

https://synbiohub.org/public/igem/BBa_K658000/annotation2264020/range2264020/1
type: Range
persistent identity: range2264020
display id: range2264020
version: 1
top level: BBa_K658000
direction: direction/forward
start: 82
end: 837
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2264024
display id: annotation2264024
version: 1
top level: BBa_K658000
location: https://synbiohub.org/public/igem/BBa_K658000/annotation2264024/range2264024/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K658000/annotation2264024/range2264024/1
type: Range
persistent identity: range2264024
display id: range2264024
version: 1
top level: BBa_K658000
direction: direction/forward
start: 871
end: 950
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2264026
display id: annotation2264026
version: 1
top level: BBa_K658000
location: https://synbiohub.org/public/igem/BBa_K658000/annotation2264026/range2264026/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K658000/annotation2264026/range2264026/1
type: Range
persistent identity: range2264026
display id: range2264026
version: 1
top level: BBa_K658000
direction: direction/forward
start: 959
end: 999
orientation: inline

BBa_R0062
type: SequenceAnnotation
persistent identity: annotation2264031
display id: annotation2264031
version: 1
top level: BBa_K658000
location: https://synbiohub.org/public/igem/BBa_K658000/annotation2264031/range2264031/1
component: BBa_R0062

https://synbiohub.org/public/igem/BBa_K658000/annotation2264031/range2264031/1
type: Range
persistent identity: range2264031
display id: range2264031
version: 1
top level: BBa_K658000
direction: direction/forward
start: 1008
end: 1062
orientation: inline

lacZa-ccdB
type: ComponentDefinition
persistent identity: BBa K176003
display id: BBa_K176003
version: 1
was generated by: iGEM to SBOL conversion
description: lacZalpha-ccdB coding sequence
created: 2009-07-02T11:00:00Z
modified: 2015-07-09T11:37:13Z
mutable provenance: PCR from the plasmid pluxCcdB3(a gift from Prof. Lingchong You). Forward Primer: GTTTCT TCTAG ATG accatgattacgg attcactggccgtcgttttac Reverse Primer: GTTTCTTC CTGCAG CGGCCGC T ACTAGT A TTATTA tattccccagaacatcaggtta Digest with XbaI and SpeI, ligate into pSB1A3.
owned by: james
owned by: myers
mutable description: A fusion protein of lacZalpha(I732006) and ccdB(K145151). It should have the function of ccdB to cause E. coli cells to die, but have less toxicity because of the lacZalpha part. It should also have the function of lacZalpha as a reporter for measurement. This part is similar to J32026 but the multiple cloning sites are eliminated. It is constructed by PCR from the plasmid pluxCcdB3(a gift from Prof. Lingchong You).
discontinued: true
dominant: false
experience: experience/Works
group u list: _282_
m user id: 4206
owner id: 3908
owning group id: 9
sample status: It's complicated
status: status/Deleted
bookmark: true
mutable notes: The part is similar to BBa_J32026 and the original coding sequence in pluxCcdB3, but the multiple cloning sites are eliminated in order to avoid site-specific mutagenesis. The multiple cloning sites are from lacZalpha in pZErO-2. After eliminate the MCS, the lacZalpha part is identical to the N-terminal of the wild type gene(BBa_I732006 & BBa_I732005), and fused with the ccdB part (BBa_K145151) with a 3aa linker.
star: false
creator: Zongxiao He, Hao Jiang
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Start
sequence annotation: a part of lacZalpha (I732006)
sequence annotation: lacZalpha-ccdB
sequence annotation: 177bp and a in J32026
sequence annotation: M13 -20 primer
sequence annotation: M13 -40 primer
sequence annotation: M13 -47 primer (K176059)
sequence annotation: g and 69bp in I732006
sequence annotation: ccdB (K145151)
sequence annotation: at in K145151
sequence annotation: VRccdB primer (K176058)
sequence annotation: c in K145151
sequence annotation: Stop
sequence: https://synbiohub.org/public/igem/BBa_K176003_sequence/1

Start
type: SequenceAnnotation
persistent identity: annotation2008001
display id: annotation2008001
version: 1
top level: lacZa-ccdB
location: https://synbiohub.org/public/igem/BBa_K176003/annotation2008001/range2008001/1
role: SO:0000318
role: feature/start

https://synbiohub.org/public/igem/BBa_K176003/annotation2008001/range2008001/1
type: Range
persistent identity: range2008001
display id: range2008001
version: 1
top level: lacZa-ccdB
direction: direction/forward
start: 1
end: 3
orientation: inline

a part of lacZalpha (I732006)
type: SequenceAnnotation
persistent identity: annotation2008000
display id: annotation2008000
version: 1
top level: lacZa-ccdB
location: https://synbiohub.org/public/igem/BBa_K176003/annotation2008000/range2008000/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_K176003/annotation2008000/range2008000/1
type: Range
persistent identity: range2008000
display id: range2008000
version: 1
top level: lacZa-ccdB
direction: direction/forward
start: 1
end: 165
orientation: inline

lacZalpha-ccdB
type: SequenceAnnotation
persistent identity: annotation2020665
display id: annotation2020665
version: 1
top level: lacZa-ccdB
location: https://synbiohub.org/public/igem/BBa_K176003/annotation2020665/range2020665/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_K176003/annotation2020665/range2020665/1
type: Range
persistent identity: range2020665
display id: range2020665
version: 1
top level: lacZa-ccdB
direction: direction/forward
start: 1
end: 474
orientation: inline

177bp and a in J32026
type: SequenceAnnotation
persistent identity: annotation2008002
display id: annotation2008002
version: 1
top level: lacZa-ccdB
location: https://synbiohub.org/public/igem/BBa_K176003/annotation2008002/range2008002/1
role: feature/mutation
role: SO:0001059

https://synbiohub.org/public/igem/BBa_K176003/annotation2008002/range2008002/1
type: Range
persistent identity: range2008002
display id: range2008002
version: 1
top level: lacZa-ccdB
direction: direction/forward
start: 16
end: 16
orientation: inline

M13 -20 primer
type: SequenceAnnotation
persistent identity: annotation2008003
display id: annotation2008003
version: 1
top level: lacZa-ccdB
location: https://synbiohub.org/public/igem/BBa_K176003/annotation2008003/range2008003/1
role: feature/primer binding
role: SO:0005850

https://synbiohub.org/public/igem/BBa_K176003/annotation2008003/range2008003/1
type: Range
persistent identity: range2008003
display id: range2008003
version: 1
top level: lacZa-ccdB
direction: direction/reverse
start: 21
end: 37
orientation: inline

M13 -40 primer
type: SequenceAnnotation
persistent identity: annotation2008005
display id: annotation2008005
version: 1
top level: lacZa-ccdB
location: https://synbiohub.org/public/igem/BBa_K176003/annotation2008005/range2008005/1
role: feature/primer binding
role: SO:0005850

https://synbiohub.org/public/igem/BBa_K176003/annotation2008005/range2008005/1
type: Range
persistent identity: range2008005
display id: range2008005
version: 1
top level: lacZa-ccdB
direction: direction/reverse
start: 41
end: 57
orientation: inline

M13 -47 primer (K176059)
type: SequenceAnnotation
persistent identity: annotation2008004
display id: annotation2008004
version: 1
top level: lacZa-ccdB
location: https://synbiohub.org/public/igem/BBa_K176003/annotation2008004/range2008004/1
role: feature/primer binding
role: SO:0005850

https://synbiohub.org/public/igem/BBa_K176003/annotation2008004/range2008004/1
type: Range
persistent identity: range2008004
display id: range2008004
version: 1
top level: lacZa-ccdB
direction: direction/reverse
start: 41
end: 64
orientation: inline

g and 69bp in I732006
type: SequenceAnnotation
persistent identity: annotation2008006
display id: annotation2008006
version: 1
top level: lacZa-ccdB
location: https://synbiohub.org/public/igem/BBa_K176003/annotation2008006/range2008006/1
role: feature/s mutation
role: SO:1000008

https://synbiohub.org/public/igem/BBa_K176003/annotation2008006/range2008006/1
type: Range
persistent identity: range2008006
display id: range2008006
version: 1
top level: lacZa-ccdB
direction: direction/forward
start: 165
end: 165
orientation: inline

ccdB (K145151)
type: SequenceAnnotation
persistent identity: annotation2008008
display id: annotation2008008
version: 1
top level: lacZa-ccdB
location: https://synbiohub.org/public/igem/BBa_K176003/annotation2008008/range2008008/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_K176003/annotation2008008/range2008008/1
type: Range
persistent identity: range2008008
display id: range2008008
version: 1
top level: lacZa-ccdB
direction: direction/forward
start: 172
end: 477
orientation: inline

at in K145151
type: SequenceAnnotation
persistent identity: annotation2008007
display id: annotation2008007
version: 1
top level: lacZa-ccdB
location: https://synbiohub.org/public/igem/BBa_K176003/annotation2008007/range2008007/1
role: feature/mutation
role: SO:0001059

https://synbiohub.org/public/igem/BBa_K176003/annotation2008007/range2008007/1
type: Range
persistent identity: range2008007
display id: range2008007
version: 1
top level: lacZa-ccdB
direction: direction/forward
start: 172
end: 173
orientation: inline

VRccdB primer (K176058)
type: SequenceAnnotation
persistent identity: annotation2011971
display id: annotation2011971
version: 1
top level: lacZa-ccdB
location: https://synbiohub.org/public/igem/BBa_K176003/annotation2011971/range2011971/1
role: feature/primer binding
role: SO:0005850

https://synbiohub.org/public/igem/BBa_K176003/annotation2011971/range2011971/1
type: Range
persistent identity: range2011971
display id: range2011971
version: 1
top level: lacZa-ccdB
direction: direction/reverse
start: 188
end: 215
orientation: inline

c in K145151
type: SequenceAnnotation
persistent identity: annotation2008009
display id: annotation2008009
version: 1
top level: lacZa-ccdB
location: https://synbiohub.org/public/igem/BBa_K176003/annotation2008009/range2008009/1
role: SO:1000008
role: feature/s mutation

https://synbiohub.org/public/igem/BBa_K176003/annotation2008009/range2008009/1
type: Range
persistent identity: range2008009
display id: range2008009
version: 1
top level: lacZa-ccdB
direction: direction/forward
start: 255
end: 255
orientation: inline

Stop
type: SequenceAnnotation
persistent identity: annotation2008010
display id: annotation2008010
version: 1
top level: lacZa-ccdB
location: https://synbiohub.org/public/igem/BBa_K176003/annotation2008010/range2008010/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_K176003/annotation2008010/range2008010/1
type: Range
persistent identity: range2008010
display id: range2008010
version: 1
top level: lacZa-ccdB
direction: direction/forward
start: 475
end: 480
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

luxI
type: ComponentDefinition
persistent identity: BBa C0061
display id: BBa_C0061
version: 1
was generated by: iGEM to SBOL conversion
description: autoinducer synthetase for AHL
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:23Z
mutable provenance: V. fischeri <genbank>AF170104</genbank>
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Synthesizes 3OC6HSL, which binds to LuxR. The lux cassette of V. fischeri contains a left and a right promoter. The right promoter gives weak constitutive expression of downstream genes.This expression is up-regulated by the action of the Lux repressor, LuxR. Two molecules of LuxR protein form a complex with two molecules the signalling compound HSL. This complex binds to a palindromic site on the promoter, increasing the rate of transcription.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: An LVA tail (sequence: AANDENYALVA) was added to increase protein degradation. .
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: BBa_C0061
sequence annotation: luxI
sequence annotation: LVA
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_C0061_sequence/1

BBa_C0061
type: SequenceAnnotation
persistent identity: annotation7038
display id: annotation7038
version: 1
top level: luxI
location: https://synbiohub.org/public/igem/BBa_C0061/annotation7038/range7038/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_C0061/annotation7038/range7038/1
type: Range
persistent identity: range7038
display id: range7038
version: 1
top level: luxI
direction: direction/forward
start: 1
end: 618
orientation: inline

luxI
type: SequenceAnnotation
persistent identity: annotation1761
display id: annotation1761
version: 1
top level: luxI
location: https://synbiohub.org/public/igem/BBa_C0061/annotation1761/range1761/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_C0061/annotation1761/range1761/1
type: Range
persistent identity: range1761
display id: range1761
version: 1
top level: luxI
direction: direction/forward
start: 1
end: 579
orientation: inline

LVA
type: SequenceAnnotation
persistent identity: annotation1760
display id: annotation1760
version: 1
top level: luxI
location: https://synbiohub.org/public/igem/BBa_C0061/annotation1760/range1760/1
role: SO:0000324
role: feature/tag

https://synbiohub.org/public/igem/BBa_C0061/annotation1760/range1760/1
type: Range
persistent identity: range1760
display id: range1760
version: 1
top level: luxI
direction: direction/forward
start: 580
end: 611
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2213985
display id: annotation2213985
version: 1
top level: luxI
location: https://synbiohub.org/public/igem/BBa_C0061/annotation2213985/range2213985/1
role: SO:0000807
role: feature/barcode

https://synbiohub.org/public/igem/BBa_C0061/annotation2213985/range2213985/1
type: Range
persistent identity: range2213985
display id: range2213985
version: 1
top level: luxI
direction: direction/forward
start: 619
end: 643
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

luxr
type: ComponentDefinition
persistent identity: BBa C0062
display id: BBa_C0062
version: 1
was generated by: iGEM to SBOL conversion
description: luxR repressor/activator, (no LVA?)
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:23Z
mutable provenance: V. fischeri <genbank>AF170104</genbank>
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: In complex with HSL, LuxR binds to the Lux promoter, activating transcription from Pr <bb_part>BBa_R0062</bb_part>, and repressing transcription from Pl <bb_part>BBa_R0063</bb_part>. The lux cassette of V. fischeri contains a left and a right promoter. The right promoter gives weak constitutive expression of downstream genes.This expression is up-regulated by the action of the Lux activator, LuxR complexed to HSL. Two molecules of LuxR protein form a complex with two molecules the signalling compound homoserine lactone (HSL). This complex binds to a palindromic site on the promoter, increasing the rate of transcription.
discontinued: false
dominant: true
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: 2 silent point mutants were introduced in the coding sequence to remove internal XbaI and PstI sites. Mutation sites were chosen to replace codons commonly used in E. coli with codons used at a similar frequency.
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: luxR
sequence annotation: prefix
sequence annotation: BBa_C0062
sequence annotation: T
sequence annotation: A
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_C0062_sequence/1

luxR
type: SequenceAnnotation
persistent identity: annotation1766
display id: annotation1766
version: 1
top level: luxr
location: https://synbiohub.org/public/igem/BBa_C0062/annotation1766/range1766/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_C0062/annotation1766/range1766/1
type: Range
persistent identity: range1766
display id: range1766
version: 1
top level: luxr
direction: direction/forward
start: 1
end: 750
orientation: inline

prefix
type: SequenceAnnotation
persistent identity: annotation1762
display id: annotation1762
version: 1
top level: luxr
location: https://synbiohub.org/public/igem/BBa_C0062/annotation1762/range1762/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_C0062/annotation1762/range1762/1
type: Range
persistent identity: range1762
display id: range1762
version: 1
top level: luxr
direction: direction/forward
start: 1
end: 2
orientation: inline

BBa_C0062
type: SequenceAnnotation
persistent identity: annotation7039
display id: annotation7039
version: 1
top level: luxr
location: https://synbiohub.org/public/igem/BBa_C0062/annotation7039/range7039/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_C0062/annotation7039/range7039/1
type: Range
persistent identity: range7039
display id: range7039
version: 1
top level: luxr
direction: direction/forward
start: 1
end: 756
orientation: inline

T
type: SequenceAnnotation
persistent identity: annotation1764
display id: annotation1764
version: 1
top level: luxr
location: https://synbiohub.org/public/igem/BBa_C0062/annotation1764/range1764/1
role: feature/s mutation
role: SO:1000008

https://synbiohub.org/public/igem/BBa_C0062/annotation1764/range1764/1
type: Range
persistent identity: range1764
display id: range1764
version: 1
top level: luxr
direction: direction/forward
start: 174
end: 174
orientation: inline

A
type: SequenceAnnotation
persistent identity: annotation1765
display id: annotation1765
version: 1
top level: luxr
location: https://synbiohub.org/public/igem/BBa_C0062/annotation1765/range1765/1
role: feature/s mutation
role: SO:1000008

https://synbiohub.org/public/igem/BBa_C0062/annotation1765/range1765/1
type: Range
persistent identity: range1765
display id: range1765
version: 1
top level: luxr
direction: direction/forward
start: 492
end: 492
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2213986
display id: annotation2213986
version: 1
top level: luxr
location: https://synbiohub.org/public/igem/BBa_C0062/annotation2213986/range2213986/1
role: feature/barcode
role: SO:0000807

https://synbiohub.org/public/igem/BBa_C0062/annotation2213986/range2213986/1
type: Range
persistent identity: range2213986
display id: range2213986
version: 1
top level: luxr
direction: direction/forward
start: 757
end: 781
orientation: inline

lux pR
type: ComponentDefinition
persistent identity: BBa R0062
display id: BBa_R0062
version: 1
was generated by: iGEM to SBOL conversion
description: Promoter (luxR & HSL regulated -- lux pR)
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:15Z
mutable provenance: V. fischeri
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Promoter activated by LuxR in concert with HSL The lux cassette of V. fischeri contains a left and a right promoter. The right promoter gives weak constitutive expression of downstream genes.This expression is up-regulated by the action of the LuxR activator protein complexed with the autoinducer, 3-oxo-hexanoyl-HSL. Two molecules of LuxR protein form a complex with two molecules of the signalling compound homoserine lactone (HSL). This complex binds to a palindromic site on the promoter, increasing the rate of transcription.
discontinued: false
dominant: true
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: This promoter is based on the Vibrio fischeri quorum sensing gene promoters. Two genes LuxI and LuxR and transcribed in opposite directions as shown below. The original sequence from which the parts <bb_part>BBa_R0062</bb_part> and <bb_part>BBa_R0063</bb_part> were derived is shown in the picture below. <img src="<bb_file>Image1.gif</bb_file>" width="614" height="362">
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: BBa_R0062
sequence annotation: LuxR/HSL
sequence annotation: -35
sequence annotation: -10
sequence annotation: start
sequence: https://synbiohub.org/public/igem/BBa_R0062_sequence/1

BBa_R0062
type: SequenceAnnotation
persistent identity: annotation7070
display id: annotation7070
version: 1
top level: lux pR
location: https://synbiohub.org/public/igem/BBa_R0062/annotation7070/range7070/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_R0062/annotation7070/range7070/1
type: Range
persistent identity: range7070
display id: range7070
version: 1
top level: lux pR
direction: direction/forward
start: 1
end: 55
orientation: inline

LuxR/HSL
type: SequenceAnnotation
persistent identity: annotation2045
display id: annotation2045
version: 1
top level: lux pR
location: https://synbiohub.org/public/igem/BBa_R0062/annotation2045/range2045/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0062/annotation2045/range2045/1
type: Range
persistent identity: range2045
display id: range2045
version: 1
top level: lux pR
direction: direction/forward
start: 1
end: 20
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2046
display id: annotation2046
version: 1
top level: lux pR
location: https://synbiohub.org/public/igem/BBa_R0062/annotation2046/range2046/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0062/annotation2046/range2046/1
type: Range
persistent identity: range2046
display id: range2046
version: 1
top level: lux pR
direction: direction/forward
start: 20
end: 25
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2047
display id: annotation2047
version: 1
top level: lux pR
location: https://synbiohub.org/public/igem/BBa_R0062/annotation2047/range2047/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0062/annotation2047/range2047/1
type: Range
persistent identity: range2047
display id: range2047
version: 1
top level: lux pR
direction: direction/forward
start: 42
end: 47
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2048
display id: annotation2048
version: 1
top level: lux pR
location: https://synbiohub.org/public/igem/BBa_R0062/annotation2048/range2048/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_R0062/annotation2048/range2048/1
type: Range
persistent identity: range2048
display id: range2048
version: 1
top level: lux pR
direction: direction/forward
start: 53
end: 53
orientation: inline

https://synbiohub.org/public/igem/BBa_K658001_sequence/1
type: Sequence
persistent identity: BBa K658001 sequence
display id: BBa_K658001_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacatactagagaaagaggagaaatactagatgactataatgataaaaaaatcggattttttggcaattccatcggaggagtataaaggtattctaagtcttcgttatcaagtgtttaagcaaagacttgagtgggacttagttgtagaaaataaccttgaatcagatgagtatgataactcaaatgcagaatatatttatgcttgtgatgatactgaaaatgtaagtggatgctggcgtttattacctacaacaggtgattatatgctgaaaagtgtttttcctgaattgcttggtcaacagagtgctcccaaagatcctaatatagtcgaattaagtcgttttgctgtaggtaaaaatagctcaaagataaataactctgctagtgaaattacaatgaaactatttgaagctatatataaacacgctgttagtcaaggtattacagaatatgtaacagtaacatcaacagcaatagagcgatttttaaagcgtattaaagttccttgtcatcgtattggagacaaagaaattcatgtattaggtgatactaaatcggttgtattgtctatgcctattaatgaacagtttaaaaaagcagtcttaaatgctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagaattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacatactagagaaagaggagaaatactagatgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatcaatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacataccattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagacctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataaatactagagaaagaggagaaatactagatgaccatgattacggattcactggccgtcgttttacaacgtc…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0015_sequence/1
type: Sequence
persistent identity: BBa B0015 sequence
display id: BBa_B0015_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0011_sequence/1
type: Sequence
persistent identity: BBa R0011 sequence
display id: BBa_R0011_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcaca
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_F1610_sequence/1
type: Sequence
persistent identity: BBa F1610 sequence
display id: BBa_F1610_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatgactataatgataaaaaaatcggattttttggcaattccatcggaggagtataaaggtattctaagtcttcgttatcaagtgtttaagcaaagacttgagtgggacttagttgtagaaaataaccttgaatcagatgagtatgataactcaaatgcagaatatatttatgcttgtgatgatactgaaaatgtaagtggatgctggcgtttattacctacaacaggtgattatatgctgaaaagtgtttttcctgaattgcttggtcaacagagtgctcccaaagatcctaatatagtcgaattaagtcgttttgctgtaggtaaaaatagctcaaagataaataactctgctagtgaaattacaatgaaactatttgaagctatatataaacacgctgttagtcaaggtattacagaatatgtaacagtaacatcaacagcaatagagcgatttttaaagcgtattaaagttccttgtcatcgtattggagacaaagaaattcatgtattaggtgatactaaatcggttgtattgtctatgcctattaatgaacagtttaaaaaagcagtcttaaatgctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K658000_sequence/1
type: Sequence
persistent identity: BBa K658000 sequence
display id: BBa_K658000_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacatactagagaaagaggagaaatactagatgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatcaatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacataccattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagacctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K176003_sequence/1
type: Sequence
persistent identity: BBa K176003 sequence
display id: BBa_K176003_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaccatgattacggattcactggccgtcgttttacaacgtcgtgactgggaaaaccctggcgttacccaacttaatcgccttgcagcacatccccctttcgccagctggcgtaatagcgaagaggcccgcaccgatcgcccttcccaacagttgcgcagcctatacgtacggcagtttaaggtttacacctataaaagagagagccgttatcgtctgtttgtggatgtacagagtgatattattgacacgccggggcgacggatggtgatccccctggccagtgcacgtctgctgtcagataaagtctcccgtgaactttacccggtggtgcatatcggggatgaaagctggcgcatgatgaccaccgatatggccagtgtgccggtctccgttatcggggaagaagtggctgatctcagccaccgcgaaaatgacatcaaaaacgccattaacctgatgttctggggaatataataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_C0061_sequence/1
type: Sequence
persistent identity: BBa C0061 sequence
display id: BBa_C0061_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgactataatgataaaaaaatcggattttttggcaattccatcggaggagtataaaggtattctaagtcttcgttatcaagtgtttaagcaaagacttgagtgggacttagttgtagaaaataaccttgaatcagatgagtatgataactcaaatgcagaatatatttatgcttgtgatgatactgaaaatgtaagtggatgctggcgtttattacctacaacaggtgattatatgctgaaaagtgtttttcctgaattgcttggtcaacagagtgctcccaaagatcctaatatagtcgaattaagtcgttttgctgtaggtaaaaatagctcaaagataaataactctgctagtgaaattacaatgaaactatttgaagctatatataaacacgctgttagtcaaggtattacagaatatgtaacagtaacatcaacagcaatagagcgatttttaaagcgtattaaagttccttgtcatcgtattggagacaaagaaattcatgtattaggtgatactaaatcggttgtattgtctatgcctattaatgaacagtttaaaaaagcagtcttaaatgctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_C0062_sequence/1
type: Sequence
persistent identity: BBa C0062 sequence
display id: BBa_C0062_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatcaatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacataccattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0062_sequence/1
type: Sequence
persistent identity: BBa R0062 sequence
display id: BBa_R0062_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: acctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataaa
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z