LOCUS BBa_K658002 5291 bp DNA linear 22-Nov-2024 DEFINITION a bacteria population-control device with RBS0.6 driven by lacl+pL ACCESSION BBa_K658002 VERSION BBa_K658002.1 FEATURES Location/Qualifiers misc_feature 3..19 /label=lac O1 promoter 20..25 /label=-35 misc_feature 26..42 /label=lac O1 promoter 43..48 /label=-10 misc_feature 1..54 /label=BBa_R0011 promoter 1..54 /label=BBa_R0011 misc_feature 68..71 /label=conserved RBS 64..75 /label=BBa_B0034 misc_feature 661..692 /label=LVA CDS 82..660 /label=luxI misc_feature 82..699 /label=BBa_C0061 misc_feature 700..724 /label=Help:Barcodes CDS 82..699 /label=BBa_C0061 stem_loop 744..787 /label=stem_loop misc_feature 733..812 /label=BBa_B0010 terminator 733..812 /label=BBa_B0010 stem_loop 828..847 /label=T7 TE misc_feature 854..854 /label=stop polyA_site 848..861 /label=polya misc_feature 821..861 /label=BBa_B0012 terminator 821..861 /label=BBa_B0012 misc_feature 64..861 /label=BBa_F1610 promoter 870..904 /label=J23110 RBS 912..923 /label=B0032 CDS 932..1498 /label=Heme oxygenase RBS 1499..1513 /label=rbs CDS 1519..2265 /label=pcyA misc_feature 870..2268 /label=BBa_K322123 RBS 2282..2286 /label=RBS CDS 2292..4526 /label=Cph8 misc_feature 2277..4529 /label=BBa_K322124 misc_feature 4550..4567 /label=C1 OmpR misc_feature 4571..4588 /label=C2 OmpR misc_feature 4591..4608 /label=C3 OmpR promoter 4612..4617 /label=-35 promoter 4635..4640 /label=-10 promoter 4538..4645 /label=BBa_R0082 misc_feature 870..4645 /label=BBa_K568000 RBS 4654..4668 /label=RBS-1\Strong RBS 4661..4665 /label=RBS misc_feature 4654..4668 /label=BBa_B0030 RBS 4654..4668 /label=BBa_B0030 misc_feature 4675..4839 /label=a part of lacZalpha (I732006) misc_feature 4675..4677 /label=Start misc_feature 4690..4690 /label=177bp and a in J32026 primer_bind 4695..4711 /label=M13 -20 primer primer_bind 4715..4738 /label=M13 -47 primer (K176059) primer_bind 4715..4731 /label=M13 -40 primer misc_feature 4839..4839 /label=g and 69bp in I732006 misc_feature 4846..4847 /label=at in K145151 misc_feature 4846..5151 /label=ccdB (K145151) misc_feature 4929..4929 /label=c in K145151 misc_feature 5149..5154 /label=Stop primer_bind 4862..4889 /label=VRccdB primer (K176058) CDS 4675..5148 /label=lacZalpha-ccdB CDS 4675..5154 /label=BBa_K176003 stem_loop 5174..5217 /label=stem_loop misc_feature 5163..5242 /label=BBa_B0010 terminator 5163..5242 /label=BBa_B0010 stem_loop 5258..5277 /label=T7 TE misc_feature 5284..5284 /label=stop polyA_site 5278..5291 /label=polya misc_feature 5251..5291 /label=BBa_B0012 terminator 5251..5291 /label=BBa_B0012 terminator 5163..5291 /label=BBa_B0015 ORIGIN 1 aattgtgagc ggataacaat tgacattgtg agcggataac aagatactga gcacatacta 61 gagaaagagg agaaatacta gatgactata atgataaaaa aatcggattt tttggcaatt 121 ccatcggagg agtataaagg tattctaagt cttcgttatc aagtgtttaa gcaaagactt 181 gagtgggact tagttgtaga aaataacctt gaatcagatg agtatgataa ctcaaatgca 241 gaatatattt atgcttgtga tgatactgaa aatgtaagtg gatgctggcg tttattacct 301 acaacaggtg attatatgct gaaaagtgtt tttcctgaat tgcttggtca acagagtgct 361 cccaaagatc ctaatatagt cgaattaagt cgttttgctg taggtaaaaa tagctcaaag 421 ataaataact ctgctagtga aattacaatg aaactatttg aagctatata taaacacgct 481 gttagtcaag gtattacaga atatgtaaca gtaacatcaa cagcaataga gcgattttta 541 aagcgtatta aagttccttg tcatcgtatt ggagacaaag aaattcatgt attaggtgat 601 actaaatcgg ttgtattgtc tatgcctatt aatgaacagt ttaaaaaagc agtcttaaat 661 gctgcaaacg acgaaaacta cgctttagta gcttaataac tctgatagtg ctagtgtaga 721 tctctactag agccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc 781 gttttatctg ttgtttgtcg gtgaacgctc tctactagag tcacactggc tcaccttcgg 841 gtgggccttt ctgcgtttat atactagagt ttacggctag ctcagtccta ggtattatgc 901 tagctactag agtcacacag gaaaggtgca catggaagag gaaatggcaa aatttaagga 961 ccatcccatc ctcagccaca tttacttccc cgaactcaac cgcaaacaaa gcctagagca 1021 agacctgcaa ttctattacg gctccaactg gcggcaagaa gtgaaaattt ctgccgctgg 1081 ccaagcctat gtggaccgag tccggcaagt ggccgctacg gcccctgaat tgttggtggc 1141 ccattcctac acccgttacc tgggggatct ttccggcggt caaattctca agaaaattgc 1201 ccaaaatgcc atgaatctcc acgatggtgg cacagctttc tatgaatttg ccgacattga 1261 tgacgaaaag gcttttaaaa atacctaccg tcaagctatg aatgatctgc ccattgacca 1321 agccaccgcc gaacggattg tggatgaagc caatgacgcc tttgccatga acatgaaaat 1381 gttcaacgaa cttgaaggca acctgatcaa ggcgatcggc attatggtgt tcaacagcct 1441 cacccgtcgc cgcagtcaag gcagcaccga agttggcctc gccacctccg aaggctagtt 1501 aaagaggaga aaggatccat ggccgtcact gatttaagtt tgaccaattc ttccctgatg 1561 cctacgttga acccgatgat tcaacagttg gccctggcga tcgccgctag ttggcaaagt 1621 ttacccctca agccctatca attgccggag gatttgggct acgtagaagg ccgcctggaa 1681 ggggaaaagt tagtgattga aaatcggtgc taccaaacgc cccagtttcg caaaatgcat 1741 ttggagttgg ccaaggtggg caaagggttg gatattctcc actgtgtaat gtttcctgag 1801 cctttatacg gtctaccttt gtttggctgt gacattgtgg ccggccccgg tggagtaagt 1861 gcggctattg cggatctatc ccccacccaa agcgatcgcc aattgcccgc agcgtaccaa 1921 aaatcattgg cagagctagg ccagccagaa tttgagcaac aacgggaatt gcccccctgg 1981 ggagaaatat tttctgaata ttgtttattc atccgtccca gcaatgtcac tgaagaagaa 2041 agatttgtac aaagggtagt ggactttttg caaattcatt gtcaccaatc catcgttgcc 2101 gaacccttgt ctgaagctca aactttggag caccgtcagg ggcaaattca ttactgccaa 2161 caacaacaga aaaatgataa aacccgtcgg gtactggaaa aagcttttgg ggaagcttgg 2221 gcggaacggt atatgagcca agtcttattt gatgttatcc aataataata ctagagagga 2281 gaaaggtacc catggccacc accgtacaac tcagcgacca atccctccgt cagctagaaa 2341 ccctcgccat ccacaccgcc cacctgattc agccccacgg tttagtggtg gtcctgcaag 2401 aaccagacct caccatcagc caaattagcg ccaactgcac cggcatttta gggcgatcgc 2461 cagaggattt gttgggcaga accctagggg aagtgtttga tagctttcag attgatccca 2521 tccagagtcg cctaacggcc ggacaaatca gcagcctcaa ccccagtaaa ctttgggcgc 2581 gggtcatggg ggacgacttt gtcatttttg acggggtttt tcatcgcaac agtgacggtt 2641 tattggtatg tgaactcgag ccagcctaca cttccgataa tctgcccttc ctcggttttt 2701 atcacatggc caacgctgcc ctgaatcggt tgcgccaaca agctaatcta cgggatttct 2761 acgatgttat tgtcgaagaa gtccgccgta tgactggctt tgaccgggtg atgctatacc 2821 gctttgatga aaataaccac ggtgatgtca ttgccgaaga taaacgggat gatatggaac 2881 cctatttggg cctgcactat cccgaatcgg atattcccca acccgcccgt cggctattta 2941 tccacaaccc cattcgagta attcccgatg tttatggtgt ggcggtgccc ctgaccccag 3001 cggttaaccc cagcaccaac cgagcggtgg atttaacaga atccattctg cgcagtgcgt 3061 accattgcca cttgacctat ctgaaaaata tgggggtagg agcgtcttta accatttccc 3121 taattaagga cggccatctc tgggggctca ttgcctgcca ccatcaaacc cccaaagtaa 3181 ttccctttga actgcgtaaa gcctgcgaat tttttggtcg ggtggtgttt agcaacattt 3241 ccgcccagga agatacggaa accttcgatt accgggtgca gctggcggag catgaagcgg 3301 ttttattgga caaaatgacc acggcggcgg attttgtcga aggattaact aatcatcccg 3361 atcgcctgtt gggattaacg ggctcccagg gggcggccat ttgctttggg gaaaaattga 3421 ttttagtagg ggaaaccccg gacgagaaag cagtgcaata tttactgcaa tggttggaga 3481 atcgggaagt gcaagacgtt ttcttcacct cttccctctc acaaatttat cctgatgcag 3541 tgaattttaa atccgtggcc agtggcttat tggccattcc cattgcccgt cacaactttt 3601 tgctctggtt tcgccctgaa gtgttgcaaa cggttaattg gggcggtgac ccaaatcatg 3661 cttacgaagc tacccaggaa gacggtaaaa tcgagctcca tccccgccaa tcctttgacc 3721 tctggaaaga aattgtccga ctccaatctt tgccctggca atcggtggaa atccaaagtg 3781 ccctggccct gaaaaaggcg atcgtcaacc tcattttgcg ccaggcagaa gaattgcata 3841 tggcggctgg tgttaagcaa ctggcggatg accgcacgct gctgatggcg ggggtaagtc 3901 acgacttgcg cacgccgctg acgcgtattc gcctggcgac tgagatgatg agcgagcagg 3961 atggctatct ggcagaatcg atcaataaag atatcgaaga gtgcaacgcc atcattgagc 4021 agtttatcga ctacctgcgc accgggcagg agatgccgat ggaaatggcg gatcttaatg 4081 cagtactcgg tgaggtgatt gctgccgaaa gtggctatga gcgggaaatt gaaaccgcgc 4141 tttaccccgg cagcattgaa gtgaaaatgc acccgctgtc gatcaaacgc gcggtggcga 4201 atatggtggt caacgccgcc cgttatggca atggctgggt caaagtcagc agcggaacgg 4261 agccgaatcg cgcctggttc caggtggaag atgacggtcc gggaattgcg ccggaacaac 4321 gtaagcacct gttccagccg tttgtccgcg gcgacagtgc gcgcaccatt agcggcacgg 4381 gattagggct ggcaattgtg cagcgtatcg tggataacca taacgggatg ctggagcttg 4441 gcaccagcga gcggggcggg ctttccattc gcgcctggct gccagtgccg gtaacgcggg 4501 cgcagggcat gacaaaagaa gggtaataat actagagtcc cttgcattta cattttgaaa 4561 catctatagc gataaatgaa acatcttaaa agttttagta tcatattcgt gttggattat 4621 tctgcatttt tggggagaat ggacttacta gagattaaag aggagaaata ctagatgacc 4681 atgattacgg attcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt 4741 acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag 4801 gcccgcaccg atcgcccttc ccaacagttg cgcagcctat acgtacggca gtttaaggtt 4861 tacacctata aaagagagag ccgttatcgt ctgtttgtgg atgtacagag tgatattatt 4921 gacacgccgg ggcgacggat ggtgatcccc ctggccagtg cacgtctgct gtcagataaa 4981 gtctcccgtg aactttaccc ggtggtgcat atcggggatg aaagctggcg catgatgacc 5041 accgatatgg ccagtgtgcc ggtctccgtt atcggggaag aagtggctga tctcagccac 5101 cgcgaaaatg acatcaaaaa cgccattaac ctgatgttct ggggaatata ataatactag 5161 agccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg 5221 ttgtttgtcg gtgaacgctc tctactagag tcacactggc tcaccttcgg gtgggccttt 5281 ctgcgtttat a //