LOCUS BBa_K658005 2576 bp DNA linear 22-Dec-2024 DEFINITION a bacteria population-control device with RBS0.07 driven by lacl+pL ACCESSION BBa_K658005 VERSION BBa_K658005.1 FEATURES Location/Qualifiers misc_feature 3..19 /label=lac O1 promoter 20..25 /label=-35 misc_feature 26..42 /label=lac O1 promoter 43..48 /label=-10 misc_feature 1..54 /label=BBa_R0011 promoter 1..54 /label=BBa_R0011 misc_feature 68..71 /label=conserved RBS 64..75 /label=BBa_B0034 misc_feature 661..692 /label=LVA CDS 82..660 /label=luxI misc_feature 82..699 /label=BBa_C0061 misc_feature 700..724 /label=Help:Barcodes CDS 82..699 /label=BBa_C0061 stem_loop 744..787 /label=stem_loop misc_feature 733..812 /label=BBa_B0010 terminator 733..812 /label=BBa_B0010 stem_loop 828..847 /label=T7 TE misc_feature 854..854 /label=stop polyA_site 848..861 /label=polya misc_feature 821..861 /label=BBa_B0012 terminator 821..861 /label=BBa_B0012 misc_feature 64..861 /label=BBa_F1610 misc_feature 872..888 /label=lac O1 promoter 889..894 /label=-35 misc_feature 895..911 /label=lac O1 promoter 912..917 /label=-10 misc_feature 870..923 /label=BBa_R0011 promoter 870..923 /label=BBa_R0011 misc_feature 937..940 /label=conserved RBS 933..944 /label=BBa_B0034 misc_feature 951..952 /label=prefix misc_feature 1124..1124 /label=T misc_feature 1442..1442 /label=A CDS 951..1700 /label=luxR misc_feature 951..1706 /label=BBa_C0062 misc_feature 1707..1731 /label=Help:Barcodes CDS 951..1706 /label=BBa_C0062 stem_loop 1751..1794 /label=stem_loop misc_feature 1740..1819 /label=BBa_B0010 terminator 1740..1819 /label=BBa_B0010 stem_loop 1835..1854 /label=T7 TE misc_feature 1861..1861 /label=stop polyA_site 1855..1868 /label=polya misc_feature 1828..1868 /label=BBa_B0012 terminator 1828..1868 /label=BBa_B0012 misc_feature 1877..1896 /label=LuxR/HSL promoter 1896..1901 /label=-35 promoter 1918..1923 /label=-10 misc_feature 1929..1929 /label=start misc_feature 1877..1931 /label=BBa_R0062 promoter 1877..1931 /label=BBa_R0062 misc_feature 870..1931 /label=BBa_K658000 misc_feature 1946..1949 /label=conserved RBS 1940..1953 /label=BBa_B0031 misc_feature 1960..2124 /label=a part of lacZalpha (I732006) misc_feature 1960..1962 /label=Start misc_feature 1975..1975 /label=177bp and a in J32026 primer_bind 1980..1996 /label=M13 -20 primer primer_bind 2000..2023 /label=M13 -47 primer (K176059) primer_bind 2000..2016 /label=M13 -40 primer misc_feature 2124..2124 /label=g and 69bp in I732006 misc_feature 2131..2132 /label=at in K145151 misc_feature 2131..2436 /label=ccdB (K145151) misc_feature 2214..2214 /label=c in K145151 misc_feature 2434..2439 /label=Stop primer_bind 2147..2174 /label=VRccdB primer (K176058) CDS 1960..2433 /label=lacZalpha-ccdB CDS 1960..2439 /label=BBa_K176003 stem_loop 2459..2502 /label=stem_loop misc_feature 2448..2527 /label=BBa_B0010 terminator 2448..2527 /label=BBa_B0010 stem_loop 2543..2562 /label=T7 TE misc_feature 2569..2569 /label=stop polyA_site 2563..2576 /label=polya misc_feature 2536..2576 /label=BBa_B0012 terminator 2536..2576 /label=BBa_B0012 terminator 2448..2576 /label=BBa_B0015 ORIGIN 1 aattgtgagc ggataacaat tgacattgtg agcggataac aagatactga gcacatacta 61 gagaaagagg agaaatacta gatgactata atgataaaaa aatcggattt tttggcaatt 121 ccatcggagg agtataaagg tattctaagt cttcgttatc aagtgtttaa gcaaagactt 181 gagtgggact tagttgtaga aaataacctt gaatcagatg agtatgataa ctcaaatgca 241 gaatatattt atgcttgtga tgatactgaa aatgtaagtg gatgctggcg tttattacct 301 acaacaggtg attatatgct gaaaagtgtt tttcctgaat tgcttggtca acagagtgct 361 cccaaagatc ctaatatagt cgaattaagt cgttttgctg taggtaaaaa tagctcaaag 421 ataaataact ctgctagtga aattacaatg aaactatttg aagctatata taaacacgct 481 gttagtcaag gtattacaga atatgtaaca gtaacatcaa cagcaataga gcgattttta 541 aagcgtatta aagttccttg tcatcgtatt ggagacaaag aaattcatgt attaggtgat 601 actaaatcgg ttgtattgtc tatgcctatt aatgaacagt ttaaaaaagc agtcttaaat 661 gctgcaaacg acgaaaacta cgctttagta gcttaataac tctgatagtg ctagtgtaga 721 tctctactag agccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc 781 gttttatctg ttgtttgtcg gtgaacgctc tctactagag tcacactggc tcaccttcgg 841 gtgggccttt ctgcgtttat atactagaga attgtgagcg gataacaatt gacattgtga 901 gcggataaca agatactgag cacatactag agaaagagga gaaatactag atgaaaaaca 961 taaatgccga cgacacatac agaataatta ataaaattaa agcttgtaga agcaataatg 1021 atattaatca atgcttatct gatatgacta aaatggtaca ttgtgaatat tatttactcg 1081 cgatcattta tcctcattct atggttaaat ctgatatttc aatcctagat aattacccta 1141 aaaaatggag gcaatattat gatgacgcta atttaataaa atatgatcct atagtagatt 1201 attctaactc caatcattca ccaattaatt ggaatatatt tgaaaacaat gctgtaaata 1261 aaaaatctcc aaatgtaatt aaagaagcga aaacatcagg tcttatcact gggtttagtt 1321 tccctattca tacggctaac aatggcttcg gaatgcttag ttttgcacat tcagaaaaag 1381 acaactatat agatagttta tttttacatg cgtgtatgaa cataccatta attgttcctt 1441 ctctagttga taattatcga aaaataaata tagcaaataa taaatcaaac aacgatttaa 1501 ccaaaagaga aaaagaatgt ttagcgtggg catgcgaagg aaaaagctct tgggatattt 1561 caaaaatatt aggttgcagt gagcgtactg tcactttcca tttaaccaat gcgcaaatga 1621 aactcaatac aacaaaccgc tgccaaagta tttctaaagc aattttaaca ggagcaattg 1681 attgcccata ctttaaaaat taataacact gatagtgcta gtgtagatca ctactagagc 1741 caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg 1801 tttgtcggtg aacgctctct actagagtca cactggctca ccttcgggtg ggcctttctg 1861 cgtttatata ctagagacct gtaggatcgt acaggtttac gcaagaaaat ggtttgttat 1921 agtcgaataa atactagagt cacacaggaa acctactaga tgaccatgat tacggattca 1981 ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc 2041 cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc 2101 ccttcccaac agttgcgcag cctatacgta cggcagttta aggtttacac ctataaaaga 2161 gagagccgtt atcgtctgtt tgtggatgta cagagtgata ttattgacac gccggggcga 2221 cggatggtga tccccctggc cagtgcacgt ctgctgtcag ataaagtctc ccgtgaactt 2281 tacccggtgg tgcatatcgg ggatgaaagc tggcgcatga tgaccaccga tatggccagt 2341 gtgccggtct ccgttatcgg ggaagaagtg gctgatctca gccaccgcga aaatgacatc 2401 aaaaacgcca ttaacctgat gttctgggga atataataat actagagcca ggcatcaaat 2461 aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa 2521 cgctctctac tagagtcaca ctggctcacc ttcgggtggg cctttctgcg tttata //