BBa_K658007
1
lux pR-5
position 5 mutated promoter lux pR-5 (luxR & HSL regulated)
2011-09-30T11:00:00Z
2015-05-08T01:13:02Z
The promoter lux pR (BBa_R0062).
The position 5 mutated promoter lux pR-5 is a product of site-directed mutagenesis of promoter lux pR (BBa_R0062). The promoter lux pR is based on the Vibrio fischeri quorum sensing gene promoters. The position 5 mutated promoter lux pR-5 gives a higher expression of downstream genes in an autoinduction system. In another word, the lux pR-5 has a higher efficiency than the lux pR. Similar to promoter lux pR, the expression is up-regulated by the action of LuxR protein combined with the signal molecule HSL.
false
false
_835_
0
10207
9
Not in stock
false
By PCR method the G residue of position 5 was changed to a C based on the sequence of the promoter lux pR(BBa_R0062).
false
Yidan Hu
annotation2148167
1
start
range2148167
1
53
53
annotation2148170
1
-35
range2148170
1
20
25
annotation2148171
1
-10
range2148171
1
42
47
annotation2148168
1
K658007
range2148168
1
1
55
annotation2148169
1
LuxR/HSL
range2148169
1
1
20
BBa_K658007_sequence
1
acctctaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataaa
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z