##gff-version 3
##sequence-region BBa_K658010 1 2577
BBa_K658010	.	promoter	1	54	.	+	0	ID=BBa_R0011;Name=BBa_R0011
BBa_K658010	.	promoter	20	25	.	+	0	ID=annotation2000;Name=-35;Parent=BBa_R0011
BBa_K658010	.	promoter	43	48	.	+	0	ID=annotation2002;Name=-10;Parent=BBa_R0011
BBa_K658010	.	operator	3	19	.	+	0	ID=annotation1999;Name=lac O1;Parent=BBa_R0011
BBa_K658010	.	operator	26	42	.	+	0	ID=annotation2001;Name=lac O1;Parent=BBa_R0011
BBa_K658010	.	engineered_region	1	54	.	+	0	ID=annotation7064;Name=BBa_R0011;Parent=BBa_R0011
BBa_K658010	.	CDS	1961	2440	.	+	0	ID=BBa_K176003;Name=BBa_K176003
BBa_K658010	.	point_mutation	2125	2125	.	+	0	ID=annotation2008006;Name=g and 69bp in I732006;Parent=BBa_K176003
BBa_K658010	.	stop_codon	2435	2440	.	+	0	ID=annotation2008010;Name=Stop;Parent=BBa_K176003
BBa_K658010	.	sequence_alteration	2132	2133	.	+	0	ID=annotation2008007;Name=at in K145151;Parent=BBa_K176003
BBa_K658010	.	primer_binding_site	1981	1997	.	+	0	ID=annotation2008003;Name=M13 -20 primer;Parent=BBa_K176003
BBa_K658010	.	engineered_region	1961	2125	.	+	0	ID=annotation2008000;Name=a part of lacZalpha (I732006);Parent=BBa_K176003
BBa_K658010	.	CDS	1961	2434	.	+	0	ID=annotation2020665;Name=lacZalpha-ccdB;Parent=BBa_K176003
BBa_K658010	.	start_codon	1961	1963	.	+	0	ID=annotation2008001;Name=Start;Parent=BBa_K176003
BBa_K658010	.	point_mutation	2215	2215	.	+	0	ID=annotation2008009;Name=c in K145151;Parent=BBa_K176003
BBa_K658010	.	primer_binding_site	2148	2175	.	+	0	ID=annotation2011971;Name=VRccdB primer (K176058);Parent=BBa_K176003
BBa_K658010	.	sequence_alteration	1976	1976	.	+	0	ID=annotation2008002;Name=177bp and a in J32026;Parent=BBa_K176003
BBa_K658010	.	engineered_region	2132	2437	.	+	0	ID=annotation2008008;Name=ccdB (K145151);Parent=BBa_K176003
BBa_K658010	.	primer_binding_site	2001	2024	.	+	0	ID=annotation2008004;Name=M13 -47 primer (K176059);Parent=BBa_K176003
BBa_K658010	.	primer_binding_site	2001	2017	.	+	0	ID=annotation2008005;Name=M13 -40 primer;Parent=BBa_K176003
BBa_K658010	.	terminator	1740	1819	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_K658010	.	stem_loop	1751	1794	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K658010	.	engineered_region	1740	1819	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K658010	.	engineered_region	64	861	.	+	0	ID=BBa_F1610;Name=BBa_F1610
BBa_K658010	.	terminator	821	861	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_F1610
BBa_K658010	.	engineered_region	821	861	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K658010	.	stop_codon	854	854	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K658010	.	stem_loop	828	847	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K658010	.	polyA_site	848	861	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K658010	.	CDS	82	699	.	+	0	ID=BBa_C0061;Name=BBa_C0061;Parent=BBa_F1610
BBa_K658010	.	engineered_region	82	699	.	+	0	ID=annotation7038;Name=BBa_C0061;Parent=BBa_C0061
BBa_K658010	.	tag	661	692	.	+	0	ID=annotation1760;Name=LVA;Parent=BBa_C0061
BBa_K658010	.	engineered_tag	700	724	.	+	0	ID=annotation2213985;Name=Help:Barcodes;Parent=BBa_C0061
BBa_K658010	.	CDS	82	660	.	+	0	ID=annotation1761;Name=luxI;Parent=BBa_C0061
BBa_K658010	.	ribosome_entry_site	64	75	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_F1610
BBa_K658010	.	conserved_region	68	71	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K658010	.	terminator	733	812	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_F1610
BBa_K658010	.	stem_loop	744	787	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K658010	.	engineered_region	733	812	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K658010	.	promoter	1877	1931	.	+	0	ID=BBa_K658007;Name=BBa_K658007
BBa_K658010	.	start_codon	1929	1929	.	+	0	ID=annotation2148167;Name=start;Parent=BBa_K658007
BBa_K658010	.	promoter	1918	1923	.	+	0	ID=annotation2148171;Name=-10;Parent=BBa_K658007
BBa_K658010	.	promoter	1896	1901	.	+	0	ID=annotation2148170;Name=-35;Parent=BBa_K658007
BBa_K658010	.	non_covalent_binding_site	1877	1896	.	+	0	ID=annotation2148169;Name=LuxR/HSL;Parent=BBa_K658007
BBa_K658010	.	engineered_region	1877	1931	.	+	0	ID=annotation2148168;Name=K658007;Parent=BBa_K658007
BBa_K658010	.	terminator	1828	1868	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_K658010	.	engineered_region	1828	1868	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K658010	.	stop_codon	1861	1861	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K658010	.	stem_loop	1835	1854	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K658010	.	polyA_site	1855	1868	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K658010	.	terminator	2449	2577	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K658010	.	terminator	2449	2528	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K658010	.	stem_loop	2460	2503	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K658010	.	engineered_region	2449	2528	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K658010	.	terminator	2537	2577	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K658010	.	engineered_region	2537	2577	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K658010	.	stop_codon	2570	2570	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K658010	.	stem_loop	2544	2563	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K658010	.	polyA_site	2564	2577	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K658010	.	CDS	951	1706	.	+	0	ID=BBa_C0062;Name=BBa_C0062
BBa_K658010	.	CDS	951	1700	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_K658010	.	engineered_region	951	1706	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_K658010	.	engineered_tag	1707	1731	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_K658010	.	point_mutation	1124	1124	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_K658010	.	engineered_region	951	952	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_K658010	.	point_mutation	1442	1442	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_K658010	.	promoter	870	923	.	+	0	ID=BBa_R0011;Name=BBa_R0011
BBa_K658010	.	promoter	889	894	.	+	0	ID=annotation2000;Name=-35;Parent=BBa_R0011
BBa_K658010	.	promoter	912	917	.	+	0	ID=annotation2002;Name=-10;Parent=BBa_R0011
BBa_K658010	.	operator	872	888	.	+	0	ID=annotation1999;Name=lac O1;Parent=BBa_R0011
BBa_K658010	.	operator	895	911	.	+	0	ID=annotation2001;Name=lac O1;Parent=BBa_R0011
BBa_K658010	.	engineered_region	870	923	.	+	0	ID=annotation7064;Name=BBa_R0011;Parent=BBa_R0011
BBa_K658010	.	ribosome_entry_site	1940	1954	.	+	0	ID=BBa_B0030;Name=BBa_B0030
BBa_K658010	.	engineered_region	1940	1954	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_K658010	.	ribosome_entry_site	1947	1951	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_K658010	.	ribosome_entry_site	1940	1954	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_K658010	.	ribosome_entry_site	933	944	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K658010	.	conserved_region	937	940	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
>BBa_K658010
aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacatactagagaaagaggagaaatact
agatgactataatgataaaaaaatcggattttttggcaattccatcggaggagtataaaggtattctaagtcttcgtta
tcaagtgtttaagcaaagacttgagtgggacttagttgtagaaaataaccttgaatcagatgagtatgataactcaaat
gcagaatatatttatgcttgtgatgatactgaaaatgtaagtggatgctggcgtttattacctacaacaggtgattata
tgctgaaaagtgtttttcctgaattgcttggtcaacagagtgctcccaaagatcctaatatagtcgaattaagtcgttt
tgctgtaggtaaaaatagctcaaagataaataactctgctagtgaaattacaatgaaactatttgaagctatatataaa
cacgctgttagtcaaggtattacagaatatgtaacagtaacatcaacagcaatagagcgatttttaaagcgtattaaag
ttccttgtcatcgtattggagacaaagaaattcatgtattaggtgatactaaatcggttgtattgtctatgcctattaa
tgaacagtttaaaaaagcagtcttaaatgctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgc
tagtgtagatctctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctg
ttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagag
aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacatactagagaaagaggagaaatact
agatgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaa
tcaatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggtt
aaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatg
atcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaa
atctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaat
ggcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaaca
taccattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaac
caaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagt
gagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaag
caattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcactactaga
gccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctc
tctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagacctctaggatcgtacaggt
ttacgcaagaaaatggtttgttatagtcgaataaatactagagattaaagaggagaaatactagatgaccatgattacg
gattcactggccgtcgttttacaacgtcgtgactgggaaaaccctggcgttacccaacttaatcgccttgcagcacatc
cccctttcgccagctggcgtaatagcgaagaggcccgcaccgatcgcccttcccaacagttgcgcagcctatacgtacg
gcagtttaaggtttacacctataaaagagagagccgttatcgtctgtttgtggatgtacagagtgatattattgacacg
ccggggcgacggatggtgatccccctggccagtgcacgtctgctgtcagataaagtctcccgtgaactttacccggtgg
tgcatatcggggatgaaagctggcgcatgatgaccaccgatatggccagtgtgccggtctccgttatcggggaagaagt
ggctgatctcagccaccgcgaaaatgacatcaaaaacgccattaacctgatgttctggggaatataataatactagagc
caggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
tactagagtcacactggctcaccttcgggtgggcctttctgcgtttata