LOCUS       BBa_K658012             1931 bp    DNA     linear       22-Dec-2024
DEFINITION  3OC6HSL Sender and Receiver Device Driven by PLlac 0-1
ACCESSION   BBa_K658012
VERSION     BBa_K658012.1  
FEATURES             Location/Qualifiers
     misc_feature    3..19
                     /label=lac O1
     promoter        20..25
                     /label=-35
     misc_feature    26..42
                     /label=lac O1
     promoter        43..48
                     /label=-10
     misc_feature    1..54
                     /label=BBa_R0011
     promoter        1..54
                     /label=BBa_R0011
     misc_feature    68..71
                     /label=conserved
     RBS             64..75
                     /label=BBa_B0034
     misc_feature    661..692
                     /label=LVA
     CDS             82..660
                     /label=luxI
     misc_feature    82..699
                     /label=BBa_C0061
     misc_feature    700..724
                     /label=Help:Barcodes
     CDS             82..699
                     /label=BBa_C0061
     stem_loop       744..787
                     /label=stem_loop
     misc_feature    733..812
                     /label=BBa_B0010
     terminator      733..812
                     /label=BBa_B0010
     stem_loop       828..847
                     /label=T7 TE
     misc_feature    854..854
                     /label=stop
     polyA_site      848..861
                     /label=polya
     misc_feature    821..861
                     /label=BBa_B0012
     terminator      821..861
                     /label=BBa_B0012
     misc_feature    64..861
                     /label=BBa_F1610
     misc_feature    872..888
                     /label=lac O1
     promoter        889..894
                     /label=-35
     misc_feature    895..911
                     /label=lac O1
     promoter        912..917
                     /label=-10
     misc_feature    870..923
                     /label=BBa_R0011
     promoter        870..923
                     /label=BBa_R0011
     misc_feature    937..940
                     /label=conserved
     RBS             933..944
                     /label=BBa_B0034
     misc_feature    951..952
                     /label=prefix
     misc_feature    1124..1124
                     /label=T
     misc_feature    1442..1442
                     /label=A
     CDS             951..1700
                     /label=luxR
     misc_feature    951..1706
                     /label=BBa_C0062
     misc_feature    1707..1731
                     /label=Help:Barcodes
     CDS             951..1706
                     /label=BBa_C0062
     stem_loop       1751..1794
                     /label=stem_loop
     misc_feature    1740..1819
                     /label=BBa_B0010
     terminator      1740..1819
                     /label=BBa_B0010
     stem_loop       1835..1854
                     /label=T7 TE
     misc_feature    1861..1861
                     /label=stop
     polyA_site      1855..1868
                     /label=polya
     misc_feature    1828..1868
                     /label=BBa_B0012
     terminator      1828..1868
                     /label=BBa_B0012
     misc_feature    1877..1896
                     /label=LuxR/HSL
     promoter        1896..1901
                     /label=-35
     promoter        1918..1923
                     /label=-10
     misc_feature    1929..1929
                     /label=start
     misc_feature    1877..1931
                     /label=BBa_R0062
     promoter        1877..1931
                     /label=BBa_R0062
     misc_feature    870..1931
                     /label=BBa_K658000
ORIGIN
        1 aattgtgagc ggataacaat tgacattgtg agcggataac aagatactga gcacatacta
       61 gagaaagagg agaaatacta gatgactata atgataaaaa aatcggattt tttggcaatt
      121 ccatcggagg agtataaagg tattctaagt cttcgttatc aagtgtttaa gcaaagactt
      181 gagtgggact tagttgtaga aaataacctt gaatcagatg agtatgataa ctcaaatgca
      241 gaatatattt atgcttgtga tgatactgaa aatgtaagtg gatgctggcg tttattacct
      301 acaacaggtg attatatgct gaaaagtgtt tttcctgaat tgcttggtca acagagtgct
      361 cccaaagatc ctaatatagt cgaattaagt cgttttgctg taggtaaaaa tagctcaaag
      421 ataaataact ctgctagtga aattacaatg aaactatttg aagctatata taaacacgct
      481 gttagtcaag gtattacaga atatgtaaca gtaacatcaa cagcaataga gcgattttta
      541 aagcgtatta aagttccttg tcatcgtatt ggagacaaag aaattcatgt attaggtgat
      601 actaaatcgg ttgtattgtc tatgcctatt aatgaacagt ttaaaaaagc agtcttaaat
      661 gctgcaaacg acgaaaacta cgctttagta gcttaataac tctgatagtg ctagtgtaga
      721 tctctactag agccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc
      781 gttttatctg ttgtttgtcg gtgaacgctc tctactagag tcacactggc tcaccttcgg
      841 gtgggccttt ctgcgtttat atactagaga attgtgagcg gataacaatt gacattgtga
      901 gcggataaca agatactgag cacatactag agaaagagga gaaatactag atgaaaaaca
      961 taaatgccga cgacacatac agaataatta ataaaattaa agcttgtaga agcaataatg
     1021 atattaatca atgcttatct gatatgacta aaatggtaca ttgtgaatat tatttactcg
     1081 cgatcattta tcctcattct atggttaaat ctgatatttc aatcctagat aattacccta
     1141 aaaaatggag gcaatattat gatgacgcta atttaataaa atatgatcct atagtagatt
     1201 attctaactc caatcattca ccaattaatt ggaatatatt tgaaaacaat gctgtaaata
     1261 aaaaatctcc aaatgtaatt aaagaagcga aaacatcagg tcttatcact gggtttagtt
     1321 tccctattca tacggctaac aatggcttcg gaatgcttag ttttgcacat tcagaaaaag
     1381 acaactatat agatagttta tttttacatg cgtgtatgaa cataccatta attgttcctt
     1441 ctctagttga taattatcga aaaataaata tagcaaataa taaatcaaac aacgatttaa
     1501 ccaaaagaga aaaagaatgt ttagcgtggg catgcgaagg aaaaagctct tgggatattt
     1561 caaaaatatt aggttgcagt gagcgtactg tcactttcca tttaaccaat gcgcaaatga
     1621 aactcaatac aacaaaccgc tgccaaagta tttctaaagc aattttaaca ggagcaattg
     1681 attgcccata ctttaaaaat taataacact gatagtgcta gtgtagatca ctactagagc
     1741 caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg
     1801 tttgtcggtg aacgctctct actagagtca cactggctca ccttcgggtg ggcctttctg
     1861 cgtttatata ctagagacct gtaggatcgt acaggtttac gcaagaaaat ggtttgttat
     1921 agtcgaataa a
//