##gff-version 3
##sequence-region BBa_K658019 1 2814
BBa_K658019	.	engineered_region	1958	2814	.	+	0	ID=BBa_J04630;Name=BBa_J04630
BBa_K658019	.	terminator	2774	2814	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_J04630
BBa_K658019	.	stem_loop	2781	2800	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K658019	.	engineered_region	2774	2814	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K658019	.	stop_codon	2807	2807	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K658019	.	polyA_site	2801	2814	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K658019	.	CDS	1958	2677	.	+	0	ID=BBa_E0040;Name=BBa_E0040;Parent=BBa_J04630
BBa_K658019	.	CDS	1958	2677	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
BBa_K658019	.	terminator	2686	2765	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_J04630
BBa_K658019	.	engineered_region	2686	2765	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K658019	.	stem_loop	2697	2740	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K658019	.	engineered_region	1	1931	.	+	0	ID=BBa_K658015;Name=BBa_K658015
BBa_K658019	.	promoter	1877	1931	.	+	0	ID=BBa_K658008;Name=BBa_K658008;Parent=BBa_K658015
BBa_K658019	.	promoter	1896	1901	.	+	0	ID=annotation2148173;Name=-35;Parent=BBa_K658008
BBa_K658019	.	non_covalent_binding_site	1877	1896	.	+	0	ID=annotation2148176;Name=LuxR/HSL;Parent=BBa_K658008
BBa_K658019	.	engineered_region	1877	1931	.	+	0	ID=annotation2148175;Name=K658008;Parent=BBa_K658008
BBa_K658019	.	promoter	1918	1923	.	+	0	ID=annotation2148174;Name=-10;Parent=BBa_K658008
BBa_K658019	.	start_codon	1929	1929	.	+	0	ID=annotation2148172;Name=start;Parent=BBa_K658008
BBa_K658019	.	ribosome_entry_site	933	944	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K658015
BBa_K658019	.	conserved_region	937	940	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K658019	.	promoter	1	54	.	+	0	ID=BBa_R0011;Name=BBa_R0011;Parent=BBa_K658015
BBa_K658019	.	operator	26	42	.	+	0	ID=annotation2001;Name=lac O1;Parent=BBa_R0011
BBa_K658019	.	engineered_region	1	54	.	+	0	ID=annotation7064;Name=BBa_R0011;Parent=BBa_R0011
BBa_K658019	.	operator	3	19	.	+	0	ID=annotation1999;Name=lac O1;Parent=BBa_R0011
BBa_K658019	.	promoter	20	25	.	+	0	ID=annotation2000;Name=-35;Parent=BBa_R0011
BBa_K658019	.	promoter	43	48	.	+	0	ID=annotation2002;Name=-10;Parent=BBa_R0011
BBa_K658019	.	terminator	1740	1868	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K658015
BBa_K658019	.	terminator	1828	1868	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K658019	.	stem_loop	1835	1854	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K658019	.	engineered_region	1828	1868	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K658019	.	stop_codon	1861	1861	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K658019	.	polyA_site	1855	1868	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K658019	.	terminator	1740	1819	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K658019	.	engineered_region	1740	1819	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K658019	.	stem_loop	1751	1794	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K658019	.	promoter	870	923	.	+	0	ID=BBa_R0011;Name=BBa_R0011;Parent=BBa_K658015
BBa_K658019	.	operator	895	911	.	+	0	ID=annotation2001;Name=lac O1;Parent=BBa_R0011
BBa_K658019	.	engineered_region	870	923	.	+	0	ID=annotation7064;Name=BBa_R0011;Parent=BBa_R0011
BBa_K658019	.	operator	872	888	.	+	0	ID=annotation1999;Name=lac O1;Parent=BBa_R0011
BBa_K658019	.	promoter	889	894	.	+	0	ID=annotation2000;Name=-35;Parent=BBa_R0011
BBa_K658019	.	promoter	912	917	.	+	0	ID=annotation2002;Name=-10;Parent=BBa_R0011
BBa_K658019	.	engineered_region	64	861	.	+	0	ID=BBa_F1610;Name=BBa_F1610;Parent=BBa_K658015
BBa_K658019	.	CDS	82	699	.	+	0	ID=BBa_C0061;Name=BBa_C0061;Parent=BBa_F1610
BBa_K658019	.	tag	661	692	.	+	0	ID=annotation1760;Name=LVA;Parent=BBa_C0061
BBa_K658019	.	engineered_tag	700	724	.	+	0	ID=annotation2213985;Name=Help:Barcodes;Parent=BBa_C0061
BBa_K658019	.	engineered_region	82	699	.	+	0	ID=annotation7038;Name=BBa_C0061;Parent=BBa_C0061
BBa_K658019	.	CDS	82	660	.	+	0	ID=annotation1761;Name=luxI;Parent=BBa_C0061
BBa_K658019	.	terminator	733	812	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_F1610
BBa_K658019	.	engineered_region	733	812	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K658019	.	stem_loop	744	787	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K658019	.	ribosome_entry_site	64	75	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_F1610
BBa_K658019	.	conserved_region	68	71	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K658019	.	terminator	821	861	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_F1610
BBa_K658019	.	stem_loop	828	847	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K658019	.	engineered_region	821	861	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K658019	.	stop_codon	854	854	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K658019	.	polyA_site	848	861	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K658019	.	CDS	951	1706	.	+	0	ID=BBa_C0062;Name=BBa_C0062;Parent=BBa_K658015
BBa_K658019	.	engineered_tag	1707	1731	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_K658019	.	engineered_region	951	952	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_K658019	.	CDS	951	1700	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_K658019	.	point_mutation	1124	1124	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_K658019	.	engineered_region	951	1706	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_K658019	.	point_mutation	1442	1442	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_K658019	.	ribosome_entry_site	1940	1951	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K658019	.	conserved_region	1944	1947	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
>BBa_K658019
aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacatactagagaaagaggagaaatact
agatgactataatgataaaaaaatcggattttttggcaattccatcggaggagtataaaggtattctaagtcttcgtta
tcaagtgtttaagcaaagacttgagtgggacttagttgtagaaaataaccttgaatcagatgagtatgataactcaaat
gcagaatatatttatgcttgtgatgatactgaaaatgtaagtggatgctggcgtttattacctacaacaggtgattata
tgctgaaaagtgtttttcctgaattgcttggtcaacagagtgctcccaaagatcctaatatagtcgaattaagtcgttt
tgctgtaggtaaaaatagctcaaagataaataactctgctagtgaaattacaatgaaactatttgaagctatatataaa
cacgctgttagtcaaggtattacagaatatgtaacagtaacatcaacagcaatagagcgatttttaaagcgtattaaag
ttccttgtcatcgtattggagacaaagaaattcatgtattaggtgatactaaatcggttgtattgtctatgcctattaa
tgaacagtttaaaaaagcagtcttaaatgctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgc
tagtgtagatctctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctg
ttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagag
aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacatactagagaaagaggagaaatact
agatgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaa
tcaatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggtt
aaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatg
atcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaa
atctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaat
ggcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaaca
taccattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaac
caaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagt
gagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaag
caattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcactactaga
gccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctc
tctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagacttctaggatcgtacaggt
ttacgcaagaaaatggtttgttatagtcgaataaatactagagaaagaggagaaatactagatgcgtaaaggagaagaa
cttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagg
gtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaac
acttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaag
agtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaag
tcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttgg
acacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaac
ttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatg
gccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagacca
catggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagc
caggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
tactagagtcacactggctcaccttcgggtgggcctttctgcgtttata