LOCUS BBa_K658020 3523 bp DNA linear 23-May-2024 DEFINITION iccdB->GFP ACCESSION BBa_K658020 VERSION BBa_K658020.1 FEATURES Location/Qualifiers misc_feature 3..19 /label=lac O1 promoter 20..25 /label=-35 misc_feature 26..42 /label=lac O1 promoter 43..48 /label=-10 misc_feature 1..54 /label=BBa_R0011 promoter 1..54 /label=BBa_R0011 misc_feature 68..71 /label=conserved RBS 64..75 /label=BBa_B0034 misc_feature 661..692 /label=LVA CDS 82..660 /label=luxI misc_feature 82..699 /label=BBa_C0061 misc_feature 700..724 /label=Help:Barcodes CDS 82..699 /label=BBa_C0061 stem_loop 744..787 /label=stem_loop misc_feature 733..812 /label=BBa_B0010 terminator 733..812 /label=BBa_B0010 stem_loop 828..847 /label=T7 TE misc_feature 854..854 /label=stop polyA_site 848..861 /label=polya misc_feature 821..861 /label=BBa_B0012 terminator 821..861 /label=BBa_B0012 misc_feature 64..861 /label=BBa_F1610 misc_feature 872..888 /label=lac O1 promoter 889..894 /label=-35 misc_feature 895..911 /label=lac O1 promoter 912..917 /label=-10 misc_feature 870..923 /label=BBa_R0011 promoter 870..923 /label=BBa_R0011 misc_feature 937..940 /label=conserved RBS 933..944 /label=BBa_B0034 misc_feature 951..952 /label=prefix misc_feature 1124..1124 /label=T misc_feature 1442..1442 /label=A CDS 951..1700 /label=luxR misc_feature 951..1706 /label=BBa_C0062 misc_feature 1707..1731 /label=Help:Barcodes CDS 951..1706 /label=BBa_C0062 stem_loop 1751..1794 /label=stem_loop misc_feature 1740..1819 /label=BBa_B0010 terminator 1740..1819 /label=BBa_B0010 stem_loop 1835..1854 /label=T7 TE misc_feature 1861..1861 /label=stop polyA_site 1855..1868 /label=polya misc_feature 1828..1868 /label=BBa_B0012 terminator 1828..1868 /label=BBa_B0012 misc_feature 1877..1896 /label=LuxR/HSL promoter 1896..1901 /label=-35 promoter 1918..1923 /label=-10 misc_feature 1929..1929 /label=start misc_feature 1877..1931 /label=BBa_R0062 promoter 1877..1931 /label=BBa_R0062 misc_feature 870..1931 /label=BBa_K658000 RBS 1940..1954 /label=RBS-1\Strong RBS 1947..1951 /label=RBS misc_feature 1940..1954 /label=BBa_B0030 RBS 1940..1954 /label=BBa_B0030 misc_feature 1961..2125 /label=a part of lacZalpha (I732006) misc_feature 1961..1963 /label=Start misc_feature 1976..1976 /label=177bp and a in J32026 primer_bind 1981..1997 /label=M13 -20 primer primer_bind 2001..2024 /label=M13 -47 primer (K176059) primer_bind 2001..2017 /label=M13 -40 primer misc_feature 2125..2125 /label=g and 69bp in I732006 misc_feature 2132..2133 /label=at in K145151 misc_feature 2132..2437 /label=ccdB (K145151) misc_feature 2215..2215 /label=c in K145151 misc_feature 2435..2440 /label=Stop primer_bind 2148..2175 /label=VRccdB primer (K176058) CDS 1961..2434 /label=lacZalpha-ccdB CDS 1961..2440 /label=BBa_K176003 stem_loop 2460..2503 /label=stem_loop misc_feature 2449..2528 /label=BBa_B0010 terminator 2449..2528 /label=BBa_B0010 stem_loop 2544..2563 /label=T7 TE misc_feature 2570..2570 /label=stop polyA_site 2564..2577 /label=polya misc_feature 2537..2577 /label=BBa_B0012 terminator 2537..2577 /label=BBa_B0012 terminator 2449..2577 /label=BBa_B0015 misc_feature 1..2577 /label=BBa_K658003 misc_feature 2588..2604 /label=lac O1 promoter 2605..2610 /label=-35 misc_feature 2611..2627 /label=lac O1 promoter 2628..2633 /label=-10 misc_feature 2586..2639 /label=BBa_R0011 promoter 2586..2639 /label=BBa_R0011 misc_feature 2653..2656 /label=conserved RBS 2649..2660 /label=BBa_B0034 CDS 2667..3386 /label=GFP protein CDS 2667..3386 /label=BBa_E0040 stem_loop 3406..3449 /label=stem_loop misc_feature 3395..3474 /label=BBa_B0010 terminator 3395..3474 /label=BBa_B0010 stem_loop 3490..3509 /label=T7 TE misc_feature 3516..3516 /label=stop polyA_site 3510..3523 /label=polya misc_feature 3483..3523 /label=BBa_B0012 terminator 3483..3523 /label=BBa_B0012 misc_feature 2667..3523 /label=BBa_J04630 ORIGIN 1 aattgtgagc ggataacaat tgacattgtg agcggataac aagatactga gcacatacta 61 gagaaagagg agaaatacta gatgactata atgataaaaa aatcggattt tttggcaatt 121 ccatcggagg agtataaagg tattctaagt cttcgttatc aagtgtttaa gcaaagactt 181 gagtgggact tagttgtaga aaataacctt gaatcagatg agtatgataa ctcaaatgca 241 gaatatattt atgcttgtga tgatactgaa aatgtaagtg gatgctggcg tttattacct 301 acaacaggtg attatatgct gaaaagtgtt tttcctgaat tgcttggtca acagagtgct 361 cccaaagatc ctaatatagt cgaattaagt cgttttgctg taggtaaaaa tagctcaaag 421 ataaataact ctgctagtga aattacaatg aaactatttg aagctatata taaacacgct 481 gttagtcaag gtattacaga atatgtaaca gtaacatcaa cagcaataga gcgattttta 541 aagcgtatta aagttccttg tcatcgtatt ggagacaaag aaattcatgt attaggtgat 601 actaaatcgg ttgtattgtc tatgcctatt aatgaacagt ttaaaaaagc agtcttaaat 661 gctgcaaacg acgaaaacta cgctttagta gcttaataac tctgatagtg ctagtgtaga 721 tctctactag agccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc 781 gttttatctg ttgtttgtcg gtgaacgctc tctactagag tcacactggc tcaccttcgg 841 gtgggccttt ctgcgtttat atactagaga attgtgagcg gataacaatt gacattgtga 901 gcggataaca agatactgag cacatactag agaaagagga gaaatactag atgaaaaaca 961 taaatgccga cgacacatac agaataatta ataaaattaa agcttgtaga agcaataatg 1021 atattaatca atgcttatct gatatgacta aaatggtaca ttgtgaatat tatttactcg 1081 cgatcattta tcctcattct atggttaaat ctgatatttc aatcctagat aattacccta 1141 aaaaatggag gcaatattat gatgacgcta atttaataaa atatgatcct atagtagatt 1201 attctaactc caatcattca ccaattaatt ggaatatatt tgaaaacaat gctgtaaata 1261 aaaaatctcc aaatgtaatt aaagaagcga aaacatcagg tcttatcact gggtttagtt 1321 tccctattca tacggctaac aatggcttcg gaatgcttag ttttgcacat tcagaaaaag 1381 acaactatat agatagttta tttttacatg cgtgtatgaa cataccatta attgttcctt 1441 ctctagttga taattatcga aaaataaata tagcaaataa taaatcaaac aacgatttaa 1501 ccaaaagaga aaaagaatgt ttagcgtggg catgcgaagg aaaaagctct tgggatattt 1561 caaaaatatt aggttgcagt gagcgtactg tcactttcca tttaaccaat gcgcaaatga 1621 aactcaatac aacaaaccgc tgccaaagta tttctaaagc aattttaaca ggagcaattg 1681 attgcccata ctttaaaaat taataacact gatagtgcta gtgtagatca ctactagagc 1741 caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg 1801 tttgtcggtg aacgctctct actagagtca cactggctca ccttcgggtg ggcctttctg 1861 cgtttatata ctagagacct gtaggatcgt acaggtttac gcaagaaaat ggtttgttat 1921 agtcgaataa atactagaga ttaaagagga gaaatactag atgaccatga ttacggattc 1981 actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg 2041 ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg 2101 cccttcccaa cagttgcgca gcctatacgt acggcagttt aaggtttaca cctataaaag 2161 agagagccgt tatcgtctgt ttgtggatgt acagagtgat attattgaca cgccggggcg 2221 acggatggtg atccccctgg ccagtgcacg tctgctgtca gataaagtct cccgtgaact 2281 ttacccggtg gtgcatatcg gggatgaaag ctggcgcatg atgaccaccg atatggccag 2341 tgtgccggtc tccgttatcg gggaagaagt ggctgatctc agccaccgcg aaaatgacat 2401 caaaaacgcc attaacctga tgttctgggg aatataataa tactagagcc aggcatcaaa 2461 taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga 2521 acgctctcta ctagagtcac actggctcac cttcgggtgg gcctttctgc gtttatatac 2581 tagagaattg tgagcggata acaattgaca ttgtgagcgg ataacaagat actgagcaca 2641 tactagagaa agaggagaaa tactagatgc gtaaaggaga agaacttttc actggagttg 2701 tcccaattct tgttgaatta gatggtgatg ttaatgggca caaattttct gtcagtggag 2761 agggtgaagg tgatgcaaca tacggaaaac ttacccttaa atttatttgc actactggaa 2821 aactacctgt tccatggcca acacttgtca ctactttcgg ttatggtgtt caatgctttg 2881 cgagataccc agatcatatg aaacagcatg actttttcaa gagtgccatg cccgaaggtt 2941 atgtacagga aagaactata tttttcaaag atgacgggaa ctacaagaca cgtgctgaag 3001 tcaagtttga aggtgatacc cttgttaata gaatcgagtt aaaaggtatt gattttaaag 3061 aagatggaaa cattcttgga cacaaattgg aatacaacta taactcacac aatgtataca 3121 tcatggcaga caaacaaaag aatggaatca aagttaactt caaaattaga cacaacattg 3181 aagatggaag cgttcaacta gcagaccatt atcaacaaaa tactccaatt ggcgatggcc 3241 ctgtcctttt accagacaac cattacctgt ccacacaatc tgccctttcg aaagatccca 3301 acgaaaagag agaccacatg gtccttcttg agtttgtaac agctgctggg attacacatg 3361 gcatggatga actatacaaa taataatact agagccaggc atcaaataaa acgaaaggct 3421 cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctctactag 3481 agtcacactg gctcaccttc gggtgggcct ttctgcgttt ata //