##gff-version 3
##sequence-region BBa_K658022 1 1945
BBa_K658022	.	ribosome_entry_site	1071	1082	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K658022	.	conserved_region	1075	1078	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K658022	.	engineered_region	1	1062	.	+	0	ID=BBa_K658000;Name=BBa_K658000
BBa_K658022	.	promoter	1008	1062	.	+	0	ID=BBa_R0062;Name=BBa_R0062;Parent=BBa_K658000
BBa_K658022	.	engineered_region	1008	1062	.	+	0	ID=annotation7070;Name=BBa_R0062;Parent=BBa_R0062
BBa_K658022	.	promoter	1049	1054	.	+	0	ID=annotation2047;Name=-10;Parent=BBa_R0062
BBa_K658022	.	start_codon	1060	1060	.	+	0	ID=annotation2048;Name=start;Parent=BBa_R0062
BBa_K658022	.	non_covalent_binding_site	1008	1027	.	+	0	ID=annotation2045;Name=LuxR/HSL;Parent=BBa_R0062
BBa_K658022	.	promoter	1027	1032	.	+	0	ID=annotation2046;Name=-35;Parent=BBa_R0062
BBa_K658022	.	promoter	1	54	.	+	0	ID=BBa_R0011;Name=BBa_R0011;Parent=BBa_K658000
BBa_K658022	.	operator	3	19	.	+	0	ID=annotation1999;Name=lac O1;Parent=BBa_R0011
BBa_K658022	.	engineered_region	1	54	.	+	0	ID=annotation7064;Name=BBa_R0011;Parent=BBa_R0011
BBa_K658022	.	promoter	43	48	.	+	0	ID=annotation2002;Name=-10;Parent=BBa_R0011
BBa_K658022	.	operator	26	42	.	+	0	ID=annotation2001;Name=lac O1;Parent=BBa_R0011
BBa_K658022	.	promoter	20	25	.	+	0	ID=annotation2000;Name=-35;Parent=BBa_R0011
BBa_K658022	.	ribosome_entry_site	64	75	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K658000
BBa_K658022	.	conserved_region	68	71	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K658022	.	terminator	871	950	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_K658000
BBa_K658022	.	engineered_region	871	950	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K658022	.	stem_loop	882	925	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K658022	.	CDS	82	837	.	+	0	ID=BBa_C0062;Name=BBa_C0062;Parent=BBa_K658000
BBa_K658022	.	engineered_tag	838	862	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_K658022	.	engineered_region	82	83	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_K658022	.	point_mutation	573	573	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_K658022	.	engineered_region	82	837	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_K658022	.	point_mutation	255	255	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_K658022	.	CDS	82	831	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_K658022	.	terminator	959	999	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_K658000
BBa_K658022	.	engineered_region	959	999	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K658022	.	stem_loop	966	985	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K658022	.	polyA_site	986	999	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K658022	.	stop_codon	992	992	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K658022	.	engineered_region	1089	1945	.	+	0	ID=BBa_J04630;Name=BBa_J04630
BBa_K658022	.	terminator	1817	1896	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_J04630
BBa_K658022	.	engineered_region	1817	1896	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K658022	.	stem_loop	1828	1871	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K658022	.	CDS	1089	1808	.	+	0	ID=BBa_E0040;Name=BBa_E0040;Parent=BBa_J04630
BBa_K658022	.	CDS	1089	1808	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
BBa_K658022	.	terminator	1905	1945	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_J04630
BBa_K658022	.	engineered_region	1905	1945	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K658022	.	stem_loop	1912	1931	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K658022	.	polyA_site	1932	1945	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K658022	.	stop_codon	1938	1938	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
>BBa_K658022
aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacatactagagaaagaggagaaatact
agatgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaa
tcaatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggtt
aaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatg
atcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaa
atctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaat
ggcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaaca
taccattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaac
caaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagt
gagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaag
caattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcactactaga
gccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctc
tctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagacctgtaggatcgtacaggt
ttacgcaagaaaatggtttgttatagtcgaataaatactagagaaagaggagaaatactagatgcgtaaaggagaagaa
cttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagg
gtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaac
acttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaag
agtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaag
tcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttgg
acacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaac
ttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatg
gccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagacca
catggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagc
caggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
tactagagtcacactggctcaccttcgggtgggcctttctgcgtttata