BBa_K660000
1
BBa_K660000
LOV2 Domain
2011-09-15T11:00:00Z
2015-05-08T01:13:03Z
The LOV domain was originally isolated from Arabidopsis and has been codon optimised for expression in E.coli.
The LOV (Light-Oxygen-Voltage) domain is a photoreceptor that responds to blue light. In nature it was first found to be involved in the phototropism response in plants and has since been found to be present in fungi and bacteria also. It has been shown to be coupled to many domains, for example phosphodiesterase or kinases.
We are using it as a reporter due to its ability to function in anoxic conditions. This is particularly useful in biofilms and is a function that fluorescent proteins derived from GFP do not have.
false
false
_837_
0
9421
9
It's complicated
true
Illegal restriction sites were found so Site Directed Mutagenesis has to be performed.
false
Glasgow Uni 2011
annotation2135554
1
LOV2 domain
range2135554
1
24
359
BBa_K660000_sequence
1
tcgctgaaggatccaagggtaccatgatcgagaagagctttgtgattaccgacccgcgtctgccggattatccgatcatcttcgccagcgatggttttctggagctgaccgagtatagccgtgaagagattatgggccgtaatgcgcgtttcttgcagggtccagaaaccgaccaagcgacggtccagaaaatccgtgacgcaattcgcgatcaacgcgaaaccactgttcagttgatcaattacacgaagtctggcaaaaagttttggaacctgctgcatctgcaaccggtccgtgatcgcaaaggcggtctgcagtacttcattggtgtgcaactggttggttccgaccacgtttaa
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z