BBa_K660300 1 BBa_K660300 Phosphodiesterase (enzyme) 2011-09-15T11:00:00Z 2015-05-08T01:13:03Z Amplified from Pseudomonas aeruginosa PA01 Phosphodiesterases are a family of enzymes naturally present in microorganisms, which break phosphodiester bonds. The specific enzyme we are using breaks the phosphodiester bond in the second messenger nucleotide, cyclic digaunylate (cyclic-di-GMP). Cyclic-di-GMP is important in many bacterial processes, including biofillm formation and motility. The enzyme regulates signal transduction by controlling levels of the signalling molecule in cells. We are using the enzyme to control the levels of cyclic-di-GMP within the cell. We expect that the targeted expression of phosphodesiterase could be used to interfere with biofilm formation or to trigger dispersal. false false _837_ 0 9421 9 In stock false illegal restriction sites false Glasgow Uni 2011 annotation2135616 1 PDE range2135616 1 17 1128 BBa_K660300_sequence 1 cgaggtcgagcccatcgtgctgaaacgcattccggtaacccagcttcgcctcggtatgttcgtccagagcctttgcggctcgtggctggaccacccgttctggaaaagaggtggcttcctgctcgacagccaggccgacctgcagcgcctgcgcgaaagcgccgtgaaggaggtctggatcgatgccagcaagggcctcgacctcccggaggaagcggcggtctcggccgctgcggtcctgccggtcaccatgccggccggcccttcgccggcgcgggtcgccctggaggaggaaatccgccacgccgccctgctctgcagccgtgccaaggccgccgtggtcagcatgttccgcgacgcgcgcatgggccaggccatcgataccgcgcatgcatcggacctggtggacgaaatctccgcctcggtgctgcgccatcccaacgcgctgctcagcctggtacggctgaagacctcggatgaatacacctacatgcattcggtggcggtctgtgcgctgatgatcgccctcgcccgccagctcgaactgcccgacccgctggtacgcgaggcggggctggccggactgctgcacgacatcggcaagatggcggtgcccgatccgatcctgaacaagcccggcaagctcaccgacccggagttcggcctggttcgccggcatccgcagaacggcgcgcggatgctgctcgattgccgccaggtcagcgccctggtggtggatgtctgcctgcaccaccacgagcgcatcgacggcaccggctaccccttcggcctggcgcaggagcagatcagcctgctggcgcgaatgggcgcggtgtgcgacgtctacgacgcgatcacctccgaccgtccctacaagaagggctggaacgccgccgaggcgatccgacgcatggccgaatggaacgggcacttcgacccgcaggtgttccgcgccttcgtcaaggcggtgggcatctacccggtcggcgcgctggtcaggctggaaagcgggcgcctcggcgtggtcctcgaacaacacggccgctcgctgctgacgccacgggtcaaggtattcttctcggcccgctcgaaggtgccgatcccgcagcaggtggtagacctgggtagggccggacagaccgaccgaatcgtcggcttcgagccggcggaagcctggaacttccgcaatctcgacgagatgtggacgggactggccaagtccacgggctcttatttcgacgcgggaaccggaaatccgtag igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z