LOCUS       BBa_K660601              975 bp    DNA     linear       22-Nov-2024
DEFINITION  Bluf + Strong RBS + YFP +TT
ACCESSION   BBa_K660601
VERSION     BBa_K660601.1  
FEATURES             Location/Qualifiers
     promoter        28..33
                     /label=-35 box
     promoter        52..58
                     /label=-10 box
     misc_feature    34..51
                     /label=spacer
     misc_feature    53..59
                     /label=inverted repeat part 1
     misc_feature    67..73
                     /label=inverted repeat part 2
     misc_feature    64..65
                     /label=transcription start
     promoter        1..86
                     /label=BBa_K238013
     RBS             95..109
                     /label=RBS-1\Strong
     RBS             102..106
                     /label=RBS
     misc_feature    95..109
                     /label=BBa_B0030
     RBS             95..109
                     /label=BBa_B0030
     CDS             116..838
                     /label=BBa_E0030
     stem_loop       858..901
                     /label=stem_loop
     misc_feature    847..926
                     /label=BBa_B0010
     terminator      847..926
                     /label=BBa_B0010
     stem_loop       942..961
                     /label=T7 TE
     misc_feature    968..968
                     /label=stop
     polyA_site      962..975
                     /label=polya
     misc_feature    935..975
                     /label=BBa_B0012
     terminator      935..975
                     /label=BBa_B0012
     misc_feature    95..975
                     /label=BBa_I13001
ORIGIN
        1 attgcaaaaa attaatttat cattctgtac acatatttcg tacaagtttg ctattgttac
       61 ttcacttaac attgattaac attttttact agagattaaa gaggagaaat actagatggt
      121 gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga
      181 cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa
      241 gctgaccctg aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt
      301 gaccaccttc ggctacggcc tgcaatgctt cgcccgctac cccgaccaca tgaagctgca
      361 cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa
      421 ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa
      481 ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct
      541 ggagtacaac tacaacagcc acaacgtcta tatcatggcc gacaagcaga agaacggcat
      601 caaggtgaac ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca
      661 ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct
      721 gagctaccag tccgccctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct
      781 ggagttcgtg accgccgccg ggatcactct cggcatggac gagctgtaca agtaataata
      841 ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta
      901 tctgttgttt gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc
      961 ctttctgcgt ttata
//