BBa_J61101 1 BBa_J61101 Ribosome Binding Site Family Member 2007-01-28T12:00:00Z 2015-08-31T02:03:00Z N/A fix false false _95_ 0 483 95 In stock false N/A true John Anderson BBa_K660603 1 BBa_K660603 OmpF + W.RBS 2011-09-20T11:00:00Z 2015-05-08T01:13:03Z both parts originally on registry A composite part consisting of OmpF and W.RBS false false _837_ 0 9421 9 It's complicated false The small size of the ribosome binding site means that it can't be visualised on a gel. We used the AlwnI method detailed on the 2011 University of Glasgow wiki. false Glasgow Uni 2011 component2137250 1 BBa_J61101 component2137249 1 BBa_R0084 annotation2137249 1 BBa_R0084 range2137249 1 1 108 annotation2137250 1 BBa_J61101 range2137250 1 117 128 BBa_R0084 1 OmpR Promoter (OmpR, positive) 2004-01-27T12:00:00Z 2015-05-08T01:14:15Z NC_000913 E. coli K12 Released HQ 2013 Positively regulated, OmpR-controlled promoter. This promoter is taken from the upstream region of ompF. Phosphorylated OmpR binds to the three operator sites and activates transcription. false false _1_ 0 24 7 In stock false The promoter includes three OmpR binding sites: F1, F2, and F3. The promoter starts at -107 and goes up to the transcriptional start site at +1. A distant fourth binding site, F4, has been deleted. It is involved in negative regulation of ompF (Head, Tardy and Kenney, 1998), so its deletion is intended to make the promoter solely activating. The efficacy of this promoter versus the ompC upstream region (BBa_R0082) or the truncated ompC upstream region (BBa_R0083) is currently unknown. true Stephen Lee, Roshan Kumar, Joe Levine, Ziyan Chu (Polkadorks, IAP 2004) annotation301309 1 F2 OmpR range301309 1 31 48 annotation301318 1 -10 range301318 1 88 93 annotation301313 1 F3 OmpR range301313 1 51 68 annotation301317 1 -35 range301317 1 73 78 annotation301308 1 F1 OmpR range301308 1 11 28 BBa_J61101_sequence 1 aaagacaggacc BBa_R0084_sequence 1 cttaaattttacttttggttacatattttttctttttgaaaccaaatctttatctttgtagcactttcacggtagcgaaacgttagtttgaatggaaagatgcctgca BBa_K660603_sequence 1 cttaaattttacttttggttacatattttttctttttgaaaccaaatctttatctttgtagcactttcacggtagcgaaacgttagtttgaatggaaagatgcctgcatactagagaaagacaggacc igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z