LOCUS       BBa_K660604             1044 bp    DNA     linear       22-Dec-2024
DEFINITION  OmpF + sYFP + TT
ACCESSION   BBa_K660604
VERSION     BBa_K660604.1  
FEATURES             Location/Qualifiers
     misc_feature    11..28
                     /label=F1 OmpR
     misc_feature    31..48
                     /label=F2 OmpR
     misc_feature    51..68
                     /label=F3 OmpR
     promoter        73..78
                     /label=-35
     promoter        88..93
                     /label=-10
     promoter        1..108
                     /label=BBa_R0084
     RBS             117..131
                     /label=RBS-1\Strong
     RBS             124..128
                     /label=RBS
     misc_feature    117..131
                     /label=BBa_B0030
     RBS             117..131
                     /label=BBa_B0030
     CDS             138..860
                     /label=BBa_E0030
     stem_loop       880..923
                     /label=stem_loop
     misc_feature    869..948
                     /label=BBa_B0010
     terminator      869..948
                     /label=BBa_B0010
     stem_loop       964..983
                     /label=T7 TE
     misc_feature    990..990
                     /label=stop
     polyA_site      984..997
                     /label=polya
     misc_feature    957..997
                     /label=BBa_B0012
     terminator      957..997
                     /label=BBa_B0012
     misc_feature    117..997
                     /label=BBa_I13001
     misc_feature    1006..1044
                     /label=B1006
     stem_loop       1015..1036
                     /label=modified thr terminator
     polyA_site      1037..1044
                     /label=PolyA
     polyA_site      1006..1014
                     /label=PolyA
     terminator      1006..1044
                     /label=BBa_B1006
ORIGIN
        1 cttaaatttt acttttggtt acatattttt tctttttgaa accaaatctt tatctttgta
       61 gcactttcac ggtagcgaaa cgttagtttg aatggaaaga tgcctgcata ctagagatta
      121 aagaggagaa atactagatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca
      181 tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg
      241 agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc
      301 ccgtgccctg gcccaccctc gtgaccacct tcggctacgg cctgcaatgc ttcgcccgct
      361 accccgacca catgaagctg cacgacttct tcaagtccgc catgcccgaa ggctacgtcc
      421 aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt
      481 tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg
      541 gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaacgtc tatatcatgg
      601 ccgacaagca gaagaacggc atcaaggtga acttcaagat ccgccacaac atcgaggacg
      661 gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc
      721 tgctgcccga caaccactac ctgagctacc agtccgccct gagcaaagac cccaacgaga
      781 agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg
      841 acgagctgta caagtaataa tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg
      901 aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcta ctagagtcac
      961 actggctcac cttcgggtgg gcctttctgc gtttatatac tagagaaaaa aaaaccccgc
     1021 ccctgacagg gcggggtttt tttt
//
LOCUS       BBa_K660605             1044 bp    DNA     linear       22-Dec-2024
DEFINITION  OmpF + wYFP + TT
ACCESSION   BBa_K660605
VERSION     BBa_K660605.1  
FEATURES             Location/Qualifiers
     misc_feature    11..28
                     /label=F1 OmpR
     misc_feature    31..48
                     /label=F2 OmpR
     misc_feature    51..68
                     /label=F3 OmpR
     promoter        73..78
                     /label=-35
     promoter        88..93
                     /label=-10
     promoter        1..108
                     /label=BBa_R0084
     RBS             117..131
                     /label=RBS-1\Strong
     RBS             124..128
                     /label=RBS
     misc_feature    117..131
                     /label=BBa_B0030
     RBS             117..131
                     /label=BBa_B0030
     CDS             138..860
                     /label=BBa_E0030
     stem_loop       880..923
                     /label=stem_loop
     misc_feature    869..948
                     /label=BBa_B0010
     terminator      869..948
                     /label=BBa_B0010
     stem_loop       964..983
                     /label=T7 TE
     misc_feature    990..990
                     /label=stop
     polyA_site      984..997
                     /label=polya
     misc_feature    957..997
                     /label=BBa_B0012
     terminator      957..997
                     /label=BBa_B0012
     misc_feature    117..997
                     /label=BBa_I13001
     misc_feature    1006..1044
                     /label=B1006
     stem_loop       1015..1036
                     /label=modified thr terminator
     polyA_site      1037..1044
                     /label=PolyA
     polyA_site      1006..1014
                     /label=PolyA
     terminator      1006..1044
                     /label=BBa_B1006
ORIGIN
        1 cttaaatttt acttttggtt acatattttt tctttttgaa accaaatctt tatctttgta
       61 gcactttcac ggtagcgaaa cgttagtttg aatggaaaga tgcctgcata ctagagatta
      121 aagaggagaa atactagatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca
      181 tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg
      241 agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc
      301 ccgtgccctg gcccaccctc gtgaccacct tcggctacgg cctgcaatgc ttcgcccgct
      361 accccgacca catgaagctg cacgacttct tcaagtccgc catgcccgaa ggctacgtcc
      421 aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt
      481 tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg
      541 gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaacgtc tatatcatgg
      601 ccgacaagca gaagaacggc atcaaggtga acttcaagat ccgccacaac atcgaggacg
      661 gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc
      721 tgctgcccga caaccactac ctgagctacc agtccgccct gagcaaagac cccaacgaga
      781 agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg
      841 acgagctgta caagtaataa tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg
      901 aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcta ctagagtcac
      961 actggctcac cttcgggtgg gcctttctgc gtttatatac tagagaaaaa aaaaccccgc
     1021 ccctgacagg gcggggtttt tttt
//