BBa_J61101
1
BBa_J61101
Ribosome Binding Site Family Member
2007-01-28T12:00:00Z
2015-08-31T02:03:00Z
N/A
fix
false
false
_95_
0
483
95
In stock
false
N/A
true
John Anderson
BBa_K238013
1
BLp
Exact promoter of the blue light receptor
2009-08-01T11:00:00Z
2015-05-08T01:11:36Z
E.coli strain MC4100
ycgF/E regulated promoter region ycgf is a cytoplasmic receptor which responds to blue light. it contains a Blue light Using FAD-Domain (BLUF) and an EAL domain. when blue light strikes, the receptor changes conformation and dimerizes. This allows it to bind to ycgE repressor through its EAL-domain releasing the repressor from this promoter region. See reference for more info: Natalia Tschowri, Susan Busse and Regine Hengge, The BLUF-EAL protein YcgF acts as a direct anti-repressor in a blue-light response of Escherichia coli. Genes and development23: 522-534 (2009)
false
false
_332_
0
4771
9
It's complicated
true
none
false
Annelien Verfaillie
annotation2014613
1
inverted repeat part 2
range2014613
1
67
73
annotation2014609
1
-35 box
range2014609
1
28
33
annotation2014611
1
spacer
range2014611
1
34
51
annotation2014612
1
inverted repeat part 1
range2014612
1
53
59
annotation2014614
1
transcription start
range2014614
1
64
65
annotation2014610
1
-10 box
range2014610
1
52
58
BBa_K660608
1
BBa_K660608
Bluf + W.RBS
2011-09-20T11:00:00Z
2015-05-08T01:13:03Z
parts from the registry
pBAD + Strong RBS.
Part of the Glasgow University 2011 promoter/RBS library
false
false
_837_
0
9421
9
It's complicated
false
The small size of the ribosome binding site means that it can't be visualised on a gel. We used the AlwnI method detailed on the 2011 University of Glasgow wiki.
false
Glasgow Uni 2011
component2137328
1
BBa_K238013
component2137329
1
BBa_J61101
annotation2137328
1
BBa_K238013
range2137328
1
1
86
annotation2137329
1
BBa_J61101
range2137329
1
95
106
BBa_J61101_sequence
1
aaagacaggacc
BBa_K660608_sequence
1
attgcaaaaaattaatttatcattctgtacacatatttcgtacaagtttgctattgttacttcacttaacattgattaacattttttactagagaaagacaggacc
BBa_K238013_sequence
1
attgcaaaaaattaatttatcattctgtacacatatttcgtacaagtttgctattgttacttcacttaacattgattaacattttt
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z